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TaKaRa escherichia coli jm109
Escherichia Coli Jm109, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/escherichia coli jm109/product/TaKaRa
Average 95 stars, based on 7 article reviews
Price from $9.99 to $1999.99
escherichia coli jm109 - by Bioz Stars, 2020-05
95/100 stars

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Ligation:

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: .. Since the base deletion background of the ligation products generated by E. coli DNA ligase could also be significantly reduced by the purification of the ligation products with a PCR product purification kit, we speculated that the ligation products generated by E. coli DNA ligase might also contain the ligation products of the phosphorylated linkers, or in other words, perhaps the linkers with 5′-OH ends could be phosphorylated by E. coli DNA ligase, too. ..

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: .. Ligation Products of the Complementary Linkers To explore if the linkers with 5′-OH ends could be joined by commercial T4 and E. coli DNA ligase, linkers A–B, C–D, and E–F were joined by using commercial T4 or E. coli DNA ligase. ..

Purification:

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: .. Since the base deletion background of the ligation products generated by E. coli DNA ligase could also be significantly reduced by the purification of the ligation products with a PCR product purification kit, we speculated that the ligation products generated by E. coli DNA ligase might also contain the ligation products of the phosphorylated linkers, or in other words, perhaps the linkers with 5′-OH ends could be phosphorylated by E. coli DNA ligase, too. ..

Generated:

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: .. The phosphorylation products by T4 DNA ligase were more when CIAP was inactivated at 85°C for 15 min than at 85°C for 30–60 min. We speculated that perhaps the linkers with 5′-OH could be joined by the commercial T4 or E. coli DNA ligase in 2 different manners: (i) the commercial T4 DNA ligase could phosphorylate the 5′-OH of linkers at a low efficiency, and then join them to the 3′-OH ends of other linkers; and (ii) the 5′-ends of the linkers could delete one or more nucleotide(s) spontaneously or by the possibly contaminated nucleases, and thereby generated some 5′-phosphate ends, and then these 5′-phosphate ends could be joined to the 3′-OH ends of other linkers at a low efficiency. .. Our findings may perhaps indicate that some DNA nicks with 5′-OH ends could be joined by T4 or E. coli DNA ligase even in the absence of PNK.

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: .. Since the base deletion background of the ligation products generated by E. coli DNA ligase could also be significantly reduced by the purification of the ligation products with a PCR product purification kit, we speculated that the ligation products generated by E. coli DNA ligase might also contain the ligation products of the phosphorylated linkers, or in other words, perhaps the linkers with 5′-OH ends could be phosphorylated by E. coli DNA ligase, too. ..

other:

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: But this hypothesis seems not to be supported by (i) T4 and E. coli DNA ligase are polypeptides with molecular weights of 68 and 75 kDa, respectively.

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: Our findings may perhaps indicate that some DNA nicks with 5′-OH ends could be joined by T4 or E. coli DNA ligase even in the absence of PNK.

DNA Sequencing:

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: .. To see if the linkers with 5′-OH ends could be correctly joined by T4 and E. coli DNA ligase, the third round PCR products were analyzed by DNA sequencing. .. The sequencing results revealed that linkers A–B and C–D had been joined by T4 or E. coli DNA ligase ( ).

Polymerase Chain Reaction:

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: .. Since the base deletion background of the ligation products generated by E. coli DNA ligase could also be significantly reduced by the purification of the ligation products with a PCR product purification kit, we speculated that the ligation products generated by E. coli DNA ligase might also contain the ligation products of the phosphorylated linkers, or in other words, perhaps the linkers with 5′-OH ends could be phosphorylated by E. coli DNA ligase, too. ..

Article Title: Detection of Ligation Products of DNA Linkers with 5?-OH Ends by Denaturing PAGE Silver Stain
Article Snippet: .. To see if the linkers with 5′-OH ends could be correctly joined by T4 and E. coli DNA ligase, the third round PCR products were analyzed by DNA sequencing. .. The sequencing results revealed that linkers A–B and C–D had been joined by T4 or E. coli DNA ligase ( ).

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    TaKaRa e coli jm109
    Effect of medium composition on PHA production in E. coli <t>JM109.</t>
    E Coli Jm109, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli jm109/product/TaKaRa
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    e coli jm109 - by Bioz Stars, 2020-05
    93/100 stars
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    Effect of medium composition on PHA production in E. coli JM109.

    Journal:

    Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109

    doi: 10.1128/AEM.71.8.4297-4306.2005

    Figure Lengend Snippet: Effect of medium composition on PHA production in E. coli JM109.

    Article Snippet: In order to test this, E. coli JM109 was transformed with the pTrcFabH(F87T) plasmid and either pBBR1C1GEC harboring the Pseudomonas sp. 61-3 phaC1 gene and the EcfabG gene or pBBRC1GPS harboring the Pseudomonas sp. 61-3 phaC1 gene and the PsfabG gene.

    Techniques:

    PHA production from glucose is enhanced by the coexpression of fabG genes with the Pseudomonas sp. 61-3 phaC1 gene and fabH (F87T) in E. coli JM109.

    Journal:

    Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109

    doi: 10.1128/AEM.71.8.4297-4306.2005

    Figure Lengend Snippet: PHA production from glucose is enhanced by the coexpression of fabG genes with the Pseudomonas sp. 61-3 phaC1 gene and fabH (F87T) in E. coli JM109.

    Article Snippet: In order to test this, E. coli JM109 was transformed with the pTrcFabH(F87T) plasmid and either pBBR1C1GEC harboring the Pseudomonas sp. 61-3 phaC1 gene and the EcfabG gene or pBBRC1GPS harboring the Pseudomonas sp. 61-3 phaC1 gene and the PsfabG gene.

    Techniques:

    PHA production from glucose is further enhanced by the coexpression of fabG genes with the genetically engineered PHA synthase genes and fabH (F87T) in E. coli JM109.

    Journal:

    Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109

    doi: 10.1128/AEM.71.8.4297-4306.2005

    Figure Lengend Snippet: PHA production from glucose is further enhanced by the coexpression of fabG genes with the genetically engineered PHA synthase genes and fabH (F87T) in E. coli JM109.

    Article Snippet: In order to test this, E. coli JM109 was transformed with the pTrcFabH(F87T) plasmid and either pBBR1C1GEC harboring the Pseudomonas sp. 61-3 phaC1 gene and the EcfabG gene or pBBRC1GPS harboring the Pseudomonas sp. 61-3 phaC1 gene and the PsfabG gene.

    Techniques: