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TaKaRa escherichia coli jm109
Escherichia Coli Jm109, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Clone Assay:

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China
Article Snippet: The stx 1 subtypes of STEC isolates were determined by a PCR-based subtyping method devised by . .. The complete stx 2 gene was amplified as described by , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: A Molecular Survey of Babesia Species and Detection of a New Babesia Species by DNA Related to B. venatorum from White Yaks in Tianzhu, China
Article Snippet: A final extension was performed at 72°C for 5 min. Each PCR product was electrophoresed on a 1.5% agarose gel containing 10 μL of gold view dye (SolarBio, Tianjin, China) in Tris-acetate-EDTA (TAE) buffer at 120 V for 30 min and visualized under UV light. .. The positive PCR products were cloned into the pGEM-T Easy vectors (Promega, USA) and transformed into Escherichia coli JM109 (TaKaRa, China). .. At least five positive clones were sequenced using an ABI Prism Terminator Cycle Sequencing kit and carried out on an Applied Biosystem 3730 DNA Analyzer (Sangon Biotech, Shanghai, China) to obtain consensus sequences.

Amplification:

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China
Article Snippet: The stx 1 subtypes of STEC isolates were determined by a PCR-based subtyping method devised by . .. The complete stx 2 gene was amplified as described by , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: A Molecular Survey of Babesia Species and Detection of a New Babesia Species by DNA Related to B. venatorum from White Yaks in Tianzhu, China
Article Snippet: Paragraph title: PCR Amplification of the V4 Region of the 18S rRNA Gene ... The positive PCR products were cloned into the pGEM-T Easy vectors (Promega, USA) and transformed into Escherichia coli JM109 (TaKaRa, China).

Agarose Gel Electrophoresis:

Article Title: A Molecular Survey of Babesia Species and Detection of a New Babesia Species by DNA Related to B. venatorum from White Yaks in Tianzhu, China
Article Snippet: A final extension was performed at 72°C for 5 min. Each PCR product was electrophoresed on a 1.5% agarose gel containing 10 μL of gold view dye (SolarBio, Tianjin, China) in Tris-acetate-EDTA (TAE) buffer at 120 V for 30 min and visualized under UV light. .. The positive PCR products were cloned into the pGEM-T Easy vectors (Promega, USA) and transformed into Escherichia coli JM109 (TaKaRa, China).

Ligation:

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: The PCR product was ligated into the pGEM-T easy vector (Promega, USA). .. The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight. .. White colonies selected were inoculated in LB broth supplemented with 50 µg/mL of ampicillin and grown under 150 rpm at 37°C for 8 h. Plasmids from recombinant colonies were prepared by a miniprep kit (Qiagen, USA) and sequenced with a SP6 and T7 primer pair (Macrogen, Korea).

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: The PCR product was ligated into the pGEM-T easy vector (Promega, USA). .. The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight. .. White colonies selected were inoculated in LB broth supplemented with 50 µg/mL of ampicillin and grown under 150 rpm at 37°C for 8 h. Plasmids from recombinant colonies were prepared by a miniprep kit (Qiagen, USA) and sequenced with a SP6 and T7 primer pair (Macrogen, Korea).

Mutagenesis:

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: Furthermore, these mutants also possessed the capacity to produce SCL-MCL PHA copolymer when grown in the presence of dodecanoate ( ). .. It was predicted that coexpression of highly active mutant forms of PHA synthases with the fabH (F87T) and fabG genes would further enhance SCL-MCL PHA production in E. coli JM109 from glucose. .. To test this, plasmids harboring the genetically engineered phaC1 genes alone (pBBRSTQK and pBBRSCQM), plasmids harboring the genetically engineered phaC1 genes with the EcfabG genes (pBBRSTQKGEC and pBBRSCQMGEC), and plasmids harboring the genetically engineered phaC1 genes with the PsfabG genes (pBBRSTQKGPS and pBBRSCQMGPS) were transformed with or without thepTrcFabH(F87T) plasmid into E. coli JM109.

Isolation:

Article Title: Characterization of the Bacillus subtilis ywsC Gene, Involved in ?-Polyglutamic Acid Production
Article Snippet: Bacillus subtilis IFO16449, a strain isolated from commercial fermented soybeans , was used in this study. .. E. coli JM109 and plasmids pUC19 and pHY300PLK were purchased from Takara Shuzo Co. (Kyoto, Japan).

Polymerase Chain Reaction:

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China).

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China).

Article Title: Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China
Article Snippet: The stx 1 subtypes of STEC isolates were determined by a PCR-based subtyping method devised by . .. The complete stx 2 gene was amplified as described by , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China).

Article Title: A Molecular Survey of Babesia Species and Detection of a New Babesia Species by DNA Related to B. venatorum from White Yaks in Tianzhu, China
Article Snippet: A final extension was performed at 72°C for 5 min. Each PCR product was electrophoresed on a 1.5% agarose gel containing 10 μL of gold view dye (SolarBio, Tianjin, China) in Tris-acetate-EDTA (TAE) buffer at 120 V for 30 min and visualized under UV light. .. The positive PCR products were cloned into the pGEM-T Easy vectors (Promega, USA) and transformed into Escherichia coli JM109 (TaKaRa, China). .. At least five positive clones were sequenced using an ABI Prism Terminator Cycle Sequencing kit and carried out on an Applied Biosystem 3730 DNA Analyzer (Sangon Biotech, Shanghai, China) to obtain consensus sequences.

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: The PCR product was ligated into the pGEM-T easy vector (Promega, USA). .. The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight.

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: The PCR product was ligated into the pGEM-T easy vector (Promega, USA). .. The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight.

Construct:

Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli
Article Snippet: E. coli DH5α cells (TaKaRa Bio, Inc.) were used to construct various recombinant plasmids. .. E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

Article Title: Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China
Article Snippet: The complete stx 2 gene was amplified as described by , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Concentration Assay:

Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli
Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis. .. Recombinant E. coli strains harboring various recombinant plasmids were grown in Luria-Bertani (LB) medium containing 100 μg/ml of ampicillin (Amp) at 37 ºC.

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: All transformations and DNA manipulations and PHA production were carried out using E. coli JM109 (Takara, Tokyo, Japan). .. All transformations and DNA manipulations and PHA production were carried out using E. coli JM109 (Takara, Tokyo, Japan).

Selection:

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: All transformations and DNA manipulations and PHA production were carried out using E. coli JM109 (Takara, Tokyo, Japan). .. For PHA production from nonrelated carbon sources, the strains were grown at 30°C in either Luria-Bertani (LB) medium supplemented with glucose to a final concentration of 2 mg ml−1 or M9 medium supplemented with 0.001% thiamine and glucose to a final concentration of 2 mg ml−1 .

Article Title: Biosynthetic Pathway and Genes of Chitin/Chitosan-Like Bioflocculant in the Genus Citrobacter
Article Snippet: Escherichia coli JM109 (Takara Bio, Kyoto, Japan) was also used as a host for the construction of recombinant DNA molecules. .. Escherichia coli JM109 (Takara Bio, Kyoto, Japan) was also used as a host for the construction of recombinant DNA molecules.

Activity Assay:

Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli
Article Snippet: E. coli DH5α cells (TaKaRa Bio, Inc.) were used to construct various recombinant plasmids. .. E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis. .. The wild-type P. syringae subsp. syringae MB03 (Microbial Genetic Stock Center, Wuhan, China) was used as the parent strain for the inaQ gene cloning.

Cell Culture:

Article Title: Type II restriction modification system in Ureaplasma parvum OMC-P162 strain
Article Snippet: All of the U . parvum clinical strains used in this study and ATCC700970 were cultured at 37°C in Ureaplasma medium [ ], consisting of 2.4% Mycoplasma broth base, 2.5% yeast extract, 5% horse serum, 0.04% urea, 0.01% L-cysteine hydrochloride, 0.001% phenol red, and 1,000 U/ml penicillin. .. The Escherichia coli JM109 (Takara Bio, Shiga, Japan) and C43 (DE3) (Lucigen, WI, USA) strains were used for DNA manipulation and recombinant protein expression, respectively.

Expressing:

Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli
Article Snippet: E. coli DH5α cells (TaKaRa Bio, Inc.) were used to construct various recombinant plasmids. .. E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis. .. The wild-type P. syringae subsp. syringae MB03 (Microbial Genetic Stock Center, Wuhan, China) was used as the parent strain for the inaQ gene cloning.

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: The ability of the transformed E. coli JM109 strains to accumulate SCL-MCL PHA copolymer from glucose was assessed by GC as described in Materials and Methods, and the results are shown in Table . .. Control strains harboring plasmids expressing either phaC1 (STQK) or phaC1 (SCQM) alone were unable to accumulate detectable levels of PHA in E. coli JM109. .. Strains harboring either EcfabG or PsfabG and phaC1 (STQK) or phaC1 (SCQM) were able to accumulate a small amount of P(3HB) homopolymer.

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: Because LB is an undefined medium, it was difficult to discern whether or not all of the PHA produced by the recombinant E. coli strains was a result of the glucose addition to the medium or from the incorporation of an undefined carbon source. .. Therefore, a defined medium (M9) with a known amount of glucose was used to examine whether recombinant E. coli strains harboring plasmids expressing the fabG , fabH (F87T), and either the phaC1 (STQK) or phaC1 (SCQM) genes were capable of producing SCL-MCL PHA copolymer in E. coli JM109 directly when glucose was the sole carbon source. .. The wild-type phaC1 expression plasmid was not used in this experiment because the amount of PHA accumulation in strains harboring plasmids expressing this gene is lower than it is in those strains expressing the highly active genetically engineered PHA synthase genes (Tables and ).

Article Title: Type II restriction modification system in Ureaplasma parvum OMC-P162 strain
Article Snippet: All of the U . parvum clinical strains used in this study and ATCC700970 were cultured at 37°C in Ureaplasma medium [ ], consisting of 2.4% Mycoplasma broth base, 2.5% yeast extract, 5% horse serum, 0.04% urea, 0.01% L-cysteine hydrochloride, 0.001% phenol red, and 1,000 U/ml penicillin. .. The Escherichia coli JM109 (Takara Bio, Shiga, Japan) and C43 (DE3) (Lucigen, WI, USA) strains were used for DNA manipulation and recombinant protein expression, respectively. .. These two strains were grown at 37°C in Luria–Bertani (LB) medium.

Sequencing:

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: Paragraph title: F6PPK encoding gene sequencing ... The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight.

Transformation Assay:

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: It was predicted that coexpression of highly active mutant forms of PHA synthases with the fabH (F87T) and fabG genes would further enhance SCL-MCL PHA production in E. coli JM109 from glucose. .. To test this, plasmids harboring the genetically engineered phaC1 genes alone (pBBRSTQK and pBBRSCQM), plasmids harboring the genetically engineered phaC1 genes with the EcfabG genes (pBBRSTQKGEC and pBBRSCQMGEC), and plasmids harboring the genetically engineered phaC1 genes with the PsfabG genes (pBBRSTQKGPS and pBBRSCQMGPS) were transformed with or without thepTrcFabH(F87T) plasmid into E. coli JM109. .. The relevant genotypes and phenotypes of the strains are described in Table .

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: It was predicted that the additional overexpression of fabG would further enhance the SCL-MCL monomer supply from fatty acid biosynthesis. .. In order to test this, E. coli JM109 was transformed with the pTrcFabH(F87T) plasmid and either pBBR1C1GEC harboring the Pseudomonas sp. 61-3 phaC1 gene and the EcfabG gene or pBBRC1GPS harboring the Pseudomonas sp. 61-3 phaC1 gene and the PsfabG gene. .. The fabH (F87T) gene was used because when it was coexpressed with the wild-type Pseudomonas sp. 61-3 phaC1 gene, it supplied the broadest substrate range of MCL monomers and produced the highest percentage of cellular dry weight PHA and the highest cell yield compared to other strains used in the previous study ( ).

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China
Article Snippet: The stx 1 subtypes of STEC isolates were determined by a PCR-based subtyping method devised by . .. The complete stx 2 gene was amplified as described by , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: A Molecular Survey of Babesia Species and Detection of a New Babesia Species by DNA Related to B. venatorum from White Yaks in Tianzhu, China
Article Snippet: A final extension was performed at 72°C for 5 min. Each PCR product was electrophoresed on a 1.5% agarose gel containing 10 μL of gold view dye (SolarBio, Tianjin, China) in Tris-acetate-EDTA (TAE) buffer at 120 V for 30 min and visualized under UV light. .. The positive PCR products were cloned into the pGEM-T Easy vectors (Promega, USA) and transformed into Escherichia coli JM109 (TaKaRa, China). .. At least five positive clones were sequenced using an ABI Prism Terminator Cycle Sequencing kit and carried out on an Applied Biosystem 3730 DNA Analyzer (Sangon Biotech, Shanghai, China) to obtain consensus sequences.

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: The PCR product was ligated into the pGEM-T easy vector (Promega, USA). .. The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight. .. White colonies selected were inoculated in LB broth supplemented with 50 µg/mL of ampicillin and grown under 150 rpm at 37°C for 8 h. Plasmids from recombinant colonies were prepared by a miniprep kit (Qiagen, USA) and sequenced with a SP6 and T7 primer pair (Macrogen, Korea).

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: The PCR product was ligated into the pGEM-T easy vector (Promega, USA). .. The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight. .. White colonies selected were inoculated in LB broth supplemented with 50 µg/mL of ampicillin and grown under 150 rpm at 37°C for 8 h. Plasmids from recombinant colonies were prepared by a miniprep kit (Qiagen, USA) and sequenced with a SP6 and T7 primer pair (Macrogen, Korea).

Recombinant:

Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli
Article Snippet: E. coli DH5α cells (TaKaRa Bio, Inc.) were used to construct various recombinant plasmids. .. E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: All transformations and DNA manipulations and PHA production were carried out using E. coli JM109 (Takara, Tokyo, Japan). .. For PHA production from nonrelated carbon sources, the strains were grown at 30°C in either Luria-Bertani (LB) medium supplemented with glucose to a final concentration of 2 mg ml−1 or M9 medium supplemented with 0.001% thiamine and glucose to a final concentration of 2 mg ml−1 .

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: Because LB is an undefined medium, it was difficult to discern whether or not all of the PHA produced by the recombinant E. coli strains was a result of the glucose addition to the medium or from the incorporation of an undefined carbon source. .. Therefore, a defined medium (M9) with a known amount of glucose was used to examine whether recombinant E. coli strains harboring plasmids expressing the fabG , fabH (F87T), and either the phaC1 (STQK) or phaC1 (SCQM) genes were capable of producing SCL-MCL PHA copolymer in E. coli JM109 directly when glucose was the sole carbon source. .. The wild-type phaC1 expression plasmid was not used in this experiment because the amount of PHA accumulation in strains harboring plasmids expressing this gene is lower than it is in those strains expressing the highly active genetically engineered PHA synthase genes (Tables and ).

Article Title: Type II restriction modification system in Ureaplasma parvum OMC-P162 strain
Article Snippet: All of the U . parvum clinical strains used in this study and ATCC700970 were cultured at 37°C in Ureaplasma medium [ ], consisting of 2.4% Mycoplasma broth base, 2.5% yeast extract, 5% horse serum, 0.04% urea, 0.01% L-cysteine hydrochloride, 0.001% phenol red, and 1,000 U/ml penicillin. .. The Escherichia coli JM109 (Takara Bio, Shiga, Japan) and C43 (DE3) (Lucigen, WI, USA) strains were used for DNA manipulation and recombinant protein expression, respectively. .. These two strains were grown at 37°C in Luria–Bertani (LB) medium.

Article Title: Biosynthetic Pathway and Genes of Chitin/Chitosan-Like Bioflocculant in the Genus Citrobacter
Article Snippet: Two BF-producing strains, C. freundii IFO 13545 ( ) and C. freundii GTC 09479 [ ], were used in this study. .. Escherichia coli JM109 (Takara Bio, Kyoto, Japan) was also used as a host for the construction of recombinant DNA molecules. .. Acetate medium (AM) (10 g CH3 COONa, 0.1 g yeast extract, 1.0 g (NH4 )2 SO4 , 1.0 g K2 HPO4 , 0.05 g NaCl, 0.2 g MgSO4 ·7H2 O, 0.05 g CaCl2 , and 0.01 g FeCl3 in 1 L, pH 7.2) [ ] and glucose medium (GM, to which glucose was added at 7.32 g·L−1 instead of acetate in AM to yield the same carbon content in both media) were used for the growth and BF production by Citrobacter strains, whereas LB medium [ ] was used for the cell growth of pre-cultures and for plasmid preparation.

other:

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: Overall, these results indicate that the coexpression of fabG genes with the fabH (F87T) and PHA synthase genes results in the enhanced production of SCL-MCL PHA copolymer in E. coli JM109 grown in the presence of excess glucose.

Article Title: Development of a strictly regulated xylose-induced expression system in Streptomyces
Article Snippet: Escherichia coli JM109 (Takara, Shiga, Japan) was used as the host for DNA manipulation (Table ).

Plasmid Preparation:

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: All transformations and DNA manipulations and PHA production were carried out using E. coli JM109 (Takara, Tokyo, Japan). .. For PHA production from nonrelated carbon sources, the strains were grown at 30°C in either Luria-Bertani (LB) medium supplemented with glucose to a final concentration of 2 mg ml−1 or M9 medium supplemented with 0.001% thiamine and glucose to a final concentration of 2 mg ml−1 .

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: It was predicted that coexpression of highly active mutant forms of PHA synthases with the fabH (F87T) and fabG genes would further enhance SCL-MCL PHA production in E. coli JM109 from glucose. .. To test this, plasmids harboring the genetically engineered phaC1 genes alone (pBBRSTQK and pBBRSCQM), plasmids harboring the genetically engineered phaC1 genes with the EcfabG genes (pBBRSTQKGEC and pBBRSCQMGEC), and plasmids harboring the genetically engineered phaC1 genes with the PsfabG genes (pBBRSTQKGPS and pBBRSCQMGPS) were transformed with or without thepTrcFabH(F87T) plasmid into E. coli JM109. .. The relevant genotypes and phenotypes of the strains are described in Table .

Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109
Article Snippet: It was predicted that the additional overexpression of fabG would further enhance the SCL-MCL monomer supply from fatty acid biosynthesis. .. In order to test this, E. coli JM109 was transformed with the pTrcFabH(F87T) plasmid and either pBBR1C1GEC harboring the Pseudomonas sp. 61-3 phaC1 gene and the EcfabG gene or pBBRC1GPS harboring the Pseudomonas sp. 61-3 phaC1 gene and the PsfabG gene. .. The fabH (F87T) gene was used because when it was coexpressed with the wild-type Pseudomonas sp. 61-3 phaC1 gene, it supplied the broadest substrate range of MCL monomers and produced the highest percentage of cellular dry weight PHA and the highest cell yield compared to other strains used in the previous study ( ).

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Identification and pathogenomic analysis of an Escherichia coli strain producing a novel Shiga toxin 2 subtype
Article Snippet: stx subtypes of STEC isolates were determined by the PCR-based subtyping method . .. For strains that failed to be detected by the stx 2 subtype-specific primers, the completed stx 2 gene was amplified as described previously , , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Shiga Toxin-Producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China
Article Snippet: The stx 1 subtypes of STEC isolates were determined by a PCR-based subtyping method devised by . .. The complete stx 2 gene was amplified as described by , then cloned into vector pMD18-T and transformed into E. coli JM109 (Takara, Dalian, China). .. About 10 transformants were selected for sequencing to discern multiple stx 2 subtypes in a PCR product.

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: The PCR product was ligated into the pGEM-T easy vector (Promega, USA). .. The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight.

Article Title: Development of a Rapid Method for the Screening of Conjugated Linoleic Acid (CLA)-Producing Strains of Bifidobacteriumbreve
Article Snippet: The PCR product was ligated into the pGEM-T easy vector (Promega, USA). .. The ligation product was transformed E. coli JM109 (Takara, Japan) and grown in Luria-Bertani (LB) agar supplemented with 50 µg/mL of ampicillin and X-gal (LAX agar) plate at 37°C overnight.

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    TaKaRa e coli jm109
    Flow cytometric analysis of recombinant E. coli <t>JM109/pMB117</t> and JM109/pMB102 cells expressing InaQ-GFP and InaQ-N/GFP, respectively. Cells were labeled with primary monoclonal anti-GFP antibodies, followed with secondary Cy5-conjugated IgG. The value of each histogram indicates the percentage of total Cy5-labeled fluorescent cells.
    E Coli Jm109, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Flow cytometric analysis of recombinant E. coli JM109/pMB117 and JM109/pMB102 cells expressing InaQ-GFP and InaQ-N/GFP, respectively. Cells were labeled with primary monoclonal anti-GFP antibodies, followed with secondary Cy5-conjugated IgG. The value of each histogram indicates the percentage of total Cy5-labeled fluorescent cells.

    Journal: International Journal of Biological Sciences

    Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli

    doi: 10.7150/ijbs.4524

    Figure Lengend Snippet: Flow cytometric analysis of recombinant E. coli JM109/pMB117 and JM109/pMB102 cells expressing InaQ-GFP and InaQ-N/GFP, respectively. Cells were labeled with primary monoclonal anti-GFP antibodies, followed with secondary Cy5-conjugated IgG. The value of each histogram indicates the percentage of total Cy5-labeled fluorescent cells.

    Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

    Techniques: Flow Cytometry, Recombinant, Expressing, Labeling

    Effect of IPTG concentrations and culture temperatures on the transport and surface-binding activities of InaQ-N/GFP. (A) Correlation between IPTG concentration and GFP fluorescence intensity of E. coli JM109/pMB102 expressing the InaQ-N/GFP fusion protein. (B) Residual GFP fluorescence intensity of intact cells after pronase proteolysis. (C) Whole-cell and residual GFP fluorescence intensity of the outer membrane fractions of JM109/pMB102 cells cultured at different temperatures and without/with pronase proteolysis.

    Journal: International Journal of Biological Sciences

    Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli

    doi: 10.7150/ijbs.4524

    Figure Lengend Snippet: Effect of IPTG concentrations and culture temperatures on the transport and surface-binding activities of InaQ-N/GFP. (A) Correlation between IPTG concentration and GFP fluorescence intensity of E. coli JM109/pMB102 expressing the InaQ-N/GFP fusion protein. (B) Residual GFP fluorescence intensity of intact cells after pronase proteolysis. (C) Whole-cell and residual GFP fluorescence intensity of the outer membrane fractions of JM109/pMB102 cells cultured at different temperatures and without/with pronase proteolysis.

    Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

    Techniques: Binding Assay, Concentration Assay, Fluorescence, Expressing, Cell Culture

    Expression profiles of E. coli JM109/pMB107 cells expressing InaQ-N′/GFP. (A) GFP fluorescence intensity measurements of subcellular fractions of JM109/pMB107 cells and the control strain JM109/pGFPuv expressing cellular GFP. Sol, soluble cytoplasmic fraction; OM, outer membrane fraction; IM, inner membrane fraction. Each value and error bar represents the mean of three independent experiments and its standard deviation, respectively. (B) Immunofluorescence micrographs of intact JM109/pMB107 cells. Prior to microscopic observation, the cells were treated with monoclonal anti-GFP antibodies, followed with Cy3-conjugated antibodies. (C) Flow cytometric assay of (i) JM109/pMB107 and (ii) JM109/pGFPuv (negative control). Cells were labeled with monoclonal anti-GFP primary antibodies followed with secondary Cy5-conjugated IgG. The value in each histogram indicates the percentage of total Cy5-labeled fluorescent cells.

    Journal: International Journal of Biological Sciences

    Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli

    doi: 10.7150/ijbs.4524

    Figure Lengend Snippet: Expression profiles of E. coli JM109/pMB107 cells expressing InaQ-N′/GFP. (A) GFP fluorescence intensity measurements of subcellular fractions of JM109/pMB107 cells and the control strain JM109/pGFPuv expressing cellular GFP. Sol, soluble cytoplasmic fraction; OM, outer membrane fraction; IM, inner membrane fraction. Each value and error bar represents the mean of three independent experiments and its standard deviation, respectively. (B) Immunofluorescence micrographs of intact JM109/pMB107 cells. Prior to microscopic observation, the cells were treated with monoclonal anti-GFP antibodies, followed with Cy3-conjugated antibodies. (C) Flow cytometric assay of (i) JM109/pMB107 and (ii) JM109/pGFPuv (negative control). Cells were labeled with monoclonal anti-GFP primary antibodies followed with secondary Cy5-conjugated IgG. The value in each histogram indicates the percentage of total Cy5-labeled fluorescent cells.

    Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

    Techniques: Expressing, Fluorescence, Standard Deviation, Immunofluorescence, Flow Cytometry, Negative Control, Labeling

    Pronase accessibility, SDS and EDTA sensitivity assays of (A) E. coli JM109/pMB117 cells expressing InaQ-GFP, and (B) the control strain E. coli JM109/pGFPuv expressing only cellular GFP. Relative values were based on the GFP fluorescence intensity at the initial incubation time.

    Journal: International Journal of Biological Sciences

    Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli

    doi: 10.7150/ijbs.4524

    Figure Lengend Snippet: Pronase accessibility, SDS and EDTA sensitivity assays of (A) E. coli JM109/pMB117 cells expressing InaQ-GFP, and (B) the control strain E. coli JM109/pGFPuv expressing only cellular GFP. Relative values were based on the GFP fluorescence intensity at the initial incubation time.

    Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

    Techniques: Expressing, Fluorescence, Incubation

    Flow cytometric analysis of E. coli JM109/pMB102, JM109/pMB109, and JM109/pMB110 cells. The cells were labeled with primary monoclonal anti-GFP antibodies, followed with secondary Cy5-conjugated antibodies. The value in each histogram indicates the percentage of total GFP or Cy5-labeled fluorescent cells. E. coli JM109 cells were used as the negative control.

    Journal: International Journal of Biological Sciences

    Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli

    doi: 10.7150/ijbs.4524

    Figure Lengend Snippet: Flow cytometric analysis of E. coli JM109/pMB102, JM109/pMB109, and JM109/pMB110 cells. The cells were labeled with primary monoclonal anti-GFP antibodies, followed with secondary Cy5-conjugated antibodies. The value in each histogram indicates the percentage of total GFP or Cy5-labeled fluorescent cells. E. coli JM109 cells were used as the negative control.

    Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

    Techniques: Flow Cytometry, Labeling, Negative Control

    Micrographs of E. coli JM109/pMB102, JM109/pMB109, and JM109/pMB110 expressing InaQ-N/GFP, (InaQ-N) 2 /GFP and (InaQ-N) 3 /GFP, respectively. The panels show the phase-contrast microscopy and fluorescence microscopy images using green and red emission filters. For immunofluorescence microscopy, the cells were treated with anti-GFP monoclonal antibodies, followed with Cy3-conjugated secondary antibodies. E. coli JM109 cells were used as the negative control.

    Journal: International Journal of Biological Sciences

    Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli

    doi: 10.7150/ijbs.4524

    Figure Lengend Snippet: Micrographs of E. coli JM109/pMB102, JM109/pMB109, and JM109/pMB110 expressing InaQ-N/GFP, (InaQ-N) 2 /GFP and (InaQ-N) 3 /GFP, respectively. The panels show the phase-contrast microscopy and fluorescence microscopy images using green and red emission filters. For immunofluorescence microscopy, the cells were treated with anti-GFP monoclonal antibodies, followed with Cy3-conjugated secondary antibodies. E. coli JM109 cells were used as the negative control.

    Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

    Techniques: Expressing, Microscopy, Fluorescence, Immunofluorescence, Negative Control

    Flow cytometry assay of E. coli JM109/pMB118 cells expressing InaQ-C/GFP. Prior to the assay, the cells were treated with monoclonal anti-GFP primary antibodies, followed with Cy5-conjugated secondary IgG. The value in each histogram indicates the percentage of total Cy5-labeled fluorescent cells.

    Journal: International Journal of Biological Sciences

    Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli

    doi: 10.7150/ijbs.4524

    Figure Lengend Snippet: Flow cytometry assay of E. coli JM109/pMB118 cells expressing InaQ-C/GFP. Prior to the assay, the cells were treated with monoclonal anti-GFP primary antibodies, followed with Cy5-conjugated secondary IgG. The value in each histogram indicates the percentage of total Cy5-labeled fluorescent cells.

    Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

    Techniques: Flow Cytometry, Cytometry, Expressing, Labeling

    Expression profiles of E. coli JM109/pMB102, JM109/pMB109, and JM109/pMB110 cells expressing InaQ-N/GFP, (InaQ-N) 2 /GFP and (InaQ-N) 3 /GFP, respectively. (A) Western blot analysis of the cell fraction samples. Lanes 1 and 4, JM109/pMB102; lanes 2 and 5, JM109/pMB109; lanes 3 and 6, JM109/pMB110. (B) Percentage of specific GFP fluorescence intensities of subcellular fractions. E. coli JM109/pGFPuv expressing cellular GFP was used as the negative control of protein surface-immobilization. In (A) and (B), Sol, soluble cytoplasmic fraction; OM, outer membrane fraction; IM, inner membrane fraction.

    Journal: International Journal of Biological Sciences

    Article Title: Molecular Characterization of an Ice Nucleation Protein Variant (InaQ) from Pseudomonas syringae and the Analysis of Its Transmembrane Transport Activity in Escherichia coli

    doi: 10.7150/ijbs.4524

    Figure Lengend Snippet: Expression profiles of E. coli JM109/pMB102, JM109/pMB109, and JM109/pMB110 cells expressing InaQ-N/GFP, (InaQ-N) 2 /GFP and (InaQ-N) 3 /GFP, respectively. (A) Western blot analysis of the cell fraction samples. Lanes 1 and 4, JM109/pMB102; lanes 2 and 5, JM109/pMB109; lanes 3 and 6, JM109/pMB110. (B) Percentage of specific GFP fluorescence intensities of subcellular fractions. E. coli JM109/pGFPuv expressing cellular GFP was used as the negative control of protein surface-immobilization. In (A) and (B), Sol, soluble cytoplasmic fraction; OM, outer membrane fraction; IM, inner membrane fraction.

    Article Snippet: E. coli JM109 [F′ traD 36, proAB+ lacI q . lacZ ΔM15/ Δ(lac-proAB) glnV 44 e 14- gyrA 96 recA1 relA 1 endA 1 thi hsdR 17] (TaKaRa Bio, Inc.) was used as the host strain for the expression of the target proteins and for surface-immobilization activity analysis.

    Techniques: Expressing, Western Blot, Fluorescence, Negative Control

    Effect of medium composition on PHA production in E. coli JM109.

    Journal:

    Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109

    doi: 10.1128/AEM.71.8.4297-4306.2005

    Figure Lengend Snippet: Effect of medium composition on PHA production in E. coli JM109.

    Article Snippet: All transformations and DNA manipulations and PHA production were carried out using E. coli JM109 (Takara, Tokyo, Japan).

    Techniques:

    PHA production from glucose is enhanced by the coexpression of fabG genes with the Pseudomonas sp. 61-3 phaC1 gene and fabH (F87T) in E. coli JM109.

    Journal:

    Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109

    doi: 10.1128/AEM.71.8.4297-4306.2005

    Figure Lengend Snippet: PHA production from glucose is enhanced by the coexpression of fabG genes with the Pseudomonas sp. 61-3 phaC1 gene and fabH (F87T) in E. coli JM109.

    Article Snippet: All transformations and DNA manipulations and PHA production were carried out using E. coli JM109 (Takara, Tokyo, Japan).

    Techniques:

    PHA production from glucose is further enhanced by the coexpression of fabG genes with the genetically engineered PHA synthase genes and fabH (F87T) in E. coli JM109.

    Journal:

    Article Title: Expression of 3-Ketoacyl-Acyl Carrier Protein Reductase (fabG) Genes Enhances Production of Polyhydroxyalkanoate Copolymer from Glucose in Recombinant Escherichia coli JM109

    doi: 10.1128/AEM.71.8.4297-4306.2005

    Figure Lengend Snippet: PHA production from glucose is further enhanced by the coexpression of fabG genes with the genetically engineered PHA synthase genes and fabH (F87T) in E. coli JM109.

    Article Snippet: All transformations and DNA manipulations and PHA production were carried out using E. coli JM109 (Takara, Tokyo, Japan).

    Techniques: