escherichia coli dna polymerase i  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    DNA Polymerase I E coli
    Description:
    DNA Polymerase I E coli 2 500 units
    Catalog Number:
    m0209l
    Price:
    277
    Size:
    2 500 units
    Category:
    DNA Polymerases
    Buy from Supplier


    Structured Review

    New England Biolabs escherichia coli dna polymerase i
    DNA Polymerase I E coli
    DNA Polymerase I E coli 2 500 units
    https://www.bioz.com/result/escherichia coli dna polymerase i/product/New England Biolabs
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    escherichia coli dna polymerase i - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide"

    Article Title: DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms150713111

    DSBs ( a ) and SSBs ( b – d ) generated in presence of Top2 ( a ); ETO ( b ); Top1 ( c ); and DNA-damaging agents that modify the DNA termini ( d ). Red arrow represents successful nick translation. Stop sign represents unsuccessful nick translation. Nick translation by DNA polymerase I necessitates a 3'-OH, which is not reconstituted in case of Top1 cleavage or when the DNA termini is damaged (shown by asterisk). In these cases the principal enzymes involved in processing and repair of the ends are listed below the black arrow. TDP1, tyrosyl-DNA phosphodiesterase 1, PNKP, polynucleotide kinase 3'-phosphatase, APE1, AP endonuclease I [ 17 ]
    Figure Legend Snippet: DSBs ( a ) and SSBs ( b – d ) generated in presence of Top2 ( a ); ETO ( b ); Top1 ( c ); and DNA-damaging agents that modify the DNA termini ( d ). Red arrow represents successful nick translation. Stop sign represents unsuccessful nick translation. Nick translation by DNA polymerase I necessitates a 3'-OH, which is not reconstituted in case of Top1 cleavage or when the DNA termini is damaged (shown by asterisk). In these cases the principal enzymes involved in processing and repair of the ends are listed below the black arrow. TDP1, tyrosyl-DNA phosphodiesterase 1, PNKP, polynucleotide kinase 3'-phosphatase, APE1, AP endonuclease I [ 17 ]

    Techniques Used: Generated, Nick Translation

    2) Product Images from "DNA Polymerase I Is Essential for Growth of Methylobacterium dichloromethanicum DM4 with Dichloromethane"

    Article Title: DNA Polymerase I Is Essential for Growth of Methylobacterium dichloromethanicum DM4 with Dichloromethane

    Journal: Journal of Bacteriology

    doi:

    DNA polymerase I activity of wild-type M. dichloromethanicum DM4 and of the polA mutant. Shown is an autoradiogram of cell extract protein (100 μg) of wild-type strain DM4 (lane 1), mutant DM4-1445 (lane 2), and complemented mutant DM4-1445(pME8112) (lane 3), separated by SDS-PAGE in a gel containing nicked DNA, after incubation with dideoxynucleotides and α- 32 P-labeled dCTP (see Materials and Methods). Lane M, prestained marker from the scanned gel; lanes 4 and 5, E. coli DNA polymerase I and Klenow fragment, respectively (0.02 U [∼0.1 ng] each).
    Figure Legend Snippet: DNA polymerase I activity of wild-type M. dichloromethanicum DM4 and of the polA mutant. Shown is an autoradiogram of cell extract protein (100 μg) of wild-type strain DM4 (lane 1), mutant DM4-1445 (lane 2), and complemented mutant DM4-1445(pME8112) (lane 3), separated by SDS-PAGE in a gel containing nicked DNA, after incubation with dideoxynucleotides and α- 32 P-labeled dCTP (see Materials and Methods). Lane M, prestained marker from the scanned gel; lanes 4 and 5, E. coli DNA polymerase I and Klenow fragment, respectively (0.02 U [∼0.1 ng] each).

    Techniques Used: Activity Assay, Mutagenesis, SDS Page, Incubation, Labeling, Marker

    Related Articles

    Modification:

    Article Title: Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance
    Article Snippet: .. Primer extension with Klenow fragment of DNA polymerase Primer extension experiments using large fragment (Klenow) of DNA polymerase I (New England Biolabs) were performed at 25°C using the HIV-1 tat reverse primer (5′-AATACTATGGTCCACACAACTATTGCT-3′) that was unmodified or contained a single OXP modification. ..

    Polymerase Chain Reaction:

    Article Title: Enhanced Protocol for Determining the 3? Terminus of Hepatitis C Virus
    Article Snippet: .. Fifth, the first round of PCR amplification was tried with different DNA polymerases, including rTth XL, DyNAzyme EXT, Vent (New England Biolabs), DeepVent (New England Biolabs) and Phusion High-Fidelity (New England Biolabs). ..

    Incubation:

    Article Title: DNA Break Mapping Reveals Topoisomerase II Activity Genome-Wide
    Article Snippet: .. 500 μg of DNA was incubated for 40 s at 16 °C with a mixture of 200 μM of dATP, dGTP, dCTP and 20 μM of digoxigenin-11-dUTP (Roche), 117 μM of ddATP, ddGTP, ddCTP (Roche) and 1000 units of Escherichia coli DNA Polymerase I (New England Biolabs, Ipswich, MA, USA). .. As a control for labeling, 500 μg of DNA was incubated with the same reagents except digoxigenin-11-dUTP was substituted by 20 μM of dTTP.

    Amplification:

    Article Title: Enhanced Protocol for Determining the 3? Terminus of Hepatitis C Virus
    Article Snippet: .. Fifth, the first round of PCR amplification was tried with different DNA polymerases, including rTth XL, DyNAzyme EXT, Vent (New England Biolabs), DeepVent (New England Biolabs) and Phusion High-Fidelity (New England Biolabs). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs dna polymerase i
    PAGE analysis of primer extension experiments with single OXP-modified and PDE primers. Primer extension with Klenow fragment of <t>DNA</t> <t>polymerase</t> I of nonheated ( A ) and preheated ( B ) single OXP-modified reverse primer, respectively along template 2. The extension reactions were incubated at 25°C for the indicated times after which the reaction mixtures were quenched and analyzed. ( C ) Primer extension with Taq DNA polymerase of PDE and OXP forward primers (nonheated control and preheated sample) along template oligonucleotide 1. Extension reactions were incubated at 25°C for 15 min, after which the aliquots from reaction mixtures were quenched and analyzed.
    Dna Polymerase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna polymerase i/product/New England Biolabs
    Average 99 stars, based on 266 article reviews
    Price from $9.99 to $1999.99
    dna polymerase i - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    PAGE analysis of primer extension experiments with single OXP-modified and PDE primers. Primer extension with Klenow fragment of DNA polymerase I of nonheated ( A ) and preheated ( B ) single OXP-modified reverse primer, respectively along template 2. The extension reactions were incubated at 25°C for the indicated times after which the reaction mixtures were quenched and analyzed. ( C ) Primer extension with Taq DNA polymerase of PDE and OXP forward primers (nonheated control and preheated sample) along template oligonucleotide 1. Extension reactions were incubated at 25°C for 15 min, after which the aliquots from reaction mixtures were quenched and analyzed.

    Journal: Nucleic Acids Research

    Article Title: Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance

    doi: 10.1093/nar/gkn575

    Figure Lengend Snippet: PAGE analysis of primer extension experiments with single OXP-modified and PDE primers. Primer extension with Klenow fragment of DNA polymerase I of nonheated ( A ) and preheated ( B ) single OXP-modified reverse primer, respectively along template 2. The extension reactions were incubated at 25°C for the indicated times after which the reaction mixtures were quenched and analyzed. ( C ) Primer extension with Taq DNA polymerase of PDE and OXP forward primers (nonheated control and preheated sample) along template oligonucleotide 1. Extension reactions were incubated at 25°C for 15 min, after which the aliquots from reaction mixtures were quenched and analyzed.

    Article Snippet: Primer extension with Klenow fragment of DNA polymerase Primer extension experiments using large fragment (Klenow) of DNA polymerase I (New England Biolabs) were performed at 25°C using the HIV-1 tat reverse primer (5′-AATACTATGGTCCACACAACTATTGCT-3′) that was unmodified or contained a single OXP modification.

    Techniques: Polyacrylamide Gel Electrophoresis, Modification, Incubation

    (A) RCA detection using 20 pmol of circularizable probe and 10 11 artificial DNA and RNA template. Six of seven probes were shown to detect their corresponding artificial template (Table ), the negative signal (OCP-E) could be due to

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

    doi: 10.1128/JCM.43.5.2339-2344.2005

    Figure Lengend Snippet: (A) RCA detection using 20 pmol of circularizable probe and 10 11 artificial DNA and RNA template. Six of seven probes were shown to detect their corresponding artificial template (Table ), the negative signal (OCP-E) could be due to

    Article Snippet: Two DNA polymerases were chosen to perform the RCA: Vent (exo-) DNA polymerase and Bst DNA polymerase (New England Biolabs).

    Techniques:

    Pictorial representation of the RCA method. (A) Padlock probe containing target-complementary segment hybridization to a target DNA or RNA sequence. (B) The padlock probe can be circularized by DNA ligase. (C) Ligated probe and binding of complementary

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification

    doi: 10.1128/JCM.43.5.2339-2344.2005

    Figure Lengend Snippet: Pictorial representation of the RCA method. (A) Padlock probe containing target-complementary segment hybridization to a target DNA or RNA sequence. (B) The padlock probe can be circularized by DNA ligase. (C) Ligated probe and binding of complementary

    Article Snippet: Two DNA polymerases were chosen to perform the RCA: Vent (exo-) DNA polymerase and Bst DNA polymerase (New England Biolabs).

    Techniques: Hybridization, Sequencing, Binding Assay

    Schematic diagram of a motif-mixing protocol used in this study. Initially, we designed DNA sequences for microgenes core that each encode a peptide motif to be mixed in their first reading frames, after which sense and antisense MPR primers were synthesized based on these microgenes core . These primers share 3′ sequences that enable base-pair formation between the sense and antisense primers, but contain mismatched bases at their 3′-OH ends (shown by red letters with dots). In the polymerization step, motifs can be embedded either in the sense or antisense primer. In the figure, motifs A and B are embedded in the sense primers, producing primers A S and B S , while motifs C and D are in the antisense primers, producing primers C AS and D AS . The thermal cycle reaction is carried out in the presence of these MPR primers, a thermostable, a DNA polymerase and dNTP. The resultant high molecular weight DNAs are combinatorial polymers of multiple microgenes created by stochastic base paring of the MPR primers. In some clones, nucleotide insertions or deletions allow frame shift mutations (denoted by FS), so that peptide sequences encoded by the second and third reading frames appear in the translated products.

    Journal: Nucleic Acids Research

    Article Title: Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifs

    doi: 10.1093/nar/gkm017

    Figure Lengend Snippet: Schematic diagram of a motif-mixing protocol used in this study. Initially, we designed DNA sequences for microgenes core that each encode a peptide motif to be mixed in their first reading frames, after which sense and antisense MPR primers were synthesized based on these microgenes core . These primers share 3′ sequences that enable base-pair formation between the sense and antisense primers, but contain mismatched bases at their 3′-OH ends (shown by red letters with dots). In the polymerization step, motifs can be embedded either in the sense or antisense primer. In the figure, motifs A and B are embedded in the sense primers, producing primers A S and B S , while motifs C and D are in the antisense primers, producing primers C AS and D AS . The thermal cycle reaction is carried out in the presence of these MPR primers, a thermostable, a DNA polymerase and dNTP. The resultant high molecular weight DNAs are combinatorial polymers of multiple microgenes created by stochastic base paring of the MPR primers. In some clones, nucleotide insertions or deletions allow frame shift mutations (denoted by FS), so that peptide sequences encoded by the second and third reading frames appear in the translated products.

    Article Snippet: For the experiments schematically depicted in , 0.4 µM sense- and antisense MPR primers, four dNTP (0.35 mM each) and 2.6 units of 3′–5′ exo + Vent DNA polymerase (New England Biolabs) were mixed in reaction buffer (10 mM KCl, 10 mM (NH4 )2 SO4 , 20 mM Tris-HCl, 2 mM MgSO4 , 0.1% TritonX-100, pH 8.8).

    Techniques: Synthesized, Molecular Weight, Clone Assay

    Overview of the nonhomologous random recombination (NRR) method. (A) Starting DNA sequences are randomly digested with DNase I, blunt-ended with T4 DNA polymerase, and recombined with T4 DNA ligase under conditions that strongly favor intermolecular ligation over intramolecular circularization. (B) A defined stoichiometry of hairpin DNA added to the ligation reaction controls the average length of the recombined products. The completed ligation reaction is digested with a restriction endonuclease to provide a library of double-stranded recombined DNA flanked by defined primer-binding sequences.

    Journal: Nature biotechnology

    Article Title: Nucleic acid evolution and minimization by nonhomologous random recombination

    doi: 10.1038/nbt736

    Figure Lengend Snippet: Overview of the nonhomologous random recombination (NRR) method. (A) Starting DNA sequences are randomly digested with DNase I, blunt-ended with T4 DNA polymerase, and recombined with T4 DNA ligase under conditions that strongly favor intermolecular ligation over intramolecular circularization. (B) A defined stoichiometry of hairpin DNA added to the ligation reaction controls the average length of the recombined products. The completed ligation reaction is digested with a restriction endonuclease to provide a library of double-stranded recombined DNA flanked by defined primer-binding sequences.

    Article Snippet: Restriction endonucleases, T4 DNA ligase, Vent DNA polymerase, T4 polynucleotide kinase, and T4 DNA polymerase were obtained from New England Biolabs (Beverly, MA).

    Techniques: Ligation, Binding Assay