escherichia coli dh10b  (Thermo Fisher)


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    Structured Review

    Thermo Fisher escherichia coli dh10b
    Escherichia Coli Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli dh10b/product/Thermo Fisher
    Average 92 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    escherichia coli dh10b - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    Electroporation:

    Article Title: Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada
    Article Snippet: .. The prepared plasmid DNA was used to transform E. coli DH10B (Gibco BRL, Burlington, Ontario) by electroporation ( ) to determine if plasmids that could not be transferred by conjugation, and were therefore likely to not be self-transmissible, encoded drugresistance determinants. ..

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: .. Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies). ..

    Recombinant:

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: .. Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies). ..

    Clone Assay:

    Article Title: Genetic and Biochemical Characterization of the F-ATPase Operon from Streptococcus sanguis 10904
    Article Snippet: .. E. coli DH10B (Invitrogen, Carlsbad, Calif.) , GBE180 , or JP17 ( ) was used for all cloning experiments, as indicated. .. E. coli strains were grown on Luria broth (10 g of tryptone [Difco], 5 g of yeast extract [Difco], 10 g of NaCl per liter) at 37°C ( ).

    Article Title: Exploring the Role of a Conserved Class A Residue in the ?-Loop of KPC-2 ?-Lactamase
    Article Snippet: .. Lysogeny broth (LB) minimal inhibitory concentration (MICs) determinations for β-lactams were performed on single clones of E. coli DH10B (Invitrogen) harboring pBR322- catI-bla KPC-2 or a variant. ..

    Conjugation Assay:

    Article Title: Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada
    Article Snippet: .. The prepared plasmid DNA was used to transform E. coli DH10B (Gibco BRL, Burlington, Ontario) by electroporation ( ) to determine if plasmids that could not be transferred by conjugation, and were therefore likely to not be self-transmissible, encoded drugresistance determinants. ..

    Mutagenesis:

    Article Title: Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy
    Article Snippet: .. These vectors were transformed into E. coli DH10B [endA 1 recA 1 galE 15 galK 16 nupG rpsL Δlac X74 Φ80lac ZΔM15 araD 139 Δ(ara ,leu )7697 mcrA Δ(mrr -hsdRMS -mcrBC )] cells (Invitrogen, Carlsbad, CA) as a host strain and exploited for site-directed mutagenesis using Stratagene’s Quik Change mutagenesis kit to provide bla SHV-1 E166A and bla KPC-2 E166A , respectively, as previously described. .. − E. coli DH10B cells carrying the empty pBCSK (−) vector (no bla ) were used as a control.

    Article Title: Exploring the Role of Residue 228 in Substrate and Inhibitor Recognition by VIM Metallo-β-lactamases
    Article Snippet: .. E. coli DH10B (Invitrogen, Carlsbad, CA) was used as a host strain for pBCSK (−) bla VIM-2 and to obtain a mutant library at position 228 by site saturation mutagenesis, using the QuickChange XL Site-directed Mutagenesis Kit (Stratagene). .. Briefly, primers that capture all 19 variants at MBL position 228 were designed, and mutagenic polymerase chain reactions were conducted using pBCSK (−) bla VIM-2 as a template; they were transformed into E. coli DH10B.

    Concentration Assay:

    Article Title: Exploring the Role of a Conserved Class A Residue in the ?-Loop of KPC-2 ?-Lactamase
    Article Snippet: .. Lysogeny broth (LB) minimal inhibitory concentration (MICs) determinations for β-lactams were performed on single clones of E. coli DH10B (Invitrogen) harboring pBR322- catI-bla KPC-2 or a variant. ..

    Transformation Assay:

    Article Title: Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy
    Article Snippet: .. These vectors were transformed into E. coli DH10B [endA 1 recA 1 galE 15 galK 16 nupG rpsL Δlac X74 Φ80lac ZΔM15 araD 139 Δ(ara ,leu )7697 mcrA Δ(mrr -hsdRMS -mcrBC )] cells (Invitrogen, Carlsbad, CA) as a host strain and exploited for site-directed mutagenesis using Stratagene’s Quik Change mutagenesis kit to provide bla SHV-1 E166A and bla KPC-2 E166A , respectively, as previously described. .. − E. coli DH10B cells carrying the empty pBCSK (−) vector (no bla ) were used as a control.

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: .. Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies). ..

    Variant Assay:

    Article Title: Exploring the Role of a Conserved Class A Residue in the ?-Loop of KPC-2 ?-Lactamase
    Article Snippet: .. Lysogeny broth (LB) minimal inhibitory concentration (MICs) determinations for β-lactams were performed on single clones of E. coli DH10B (Invitrogen) harboring pBR322- catI-bla KPC-2 or a variant. ..

    Plasmid Preparation:

    Article Title: Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada
    Article Snippet: .. The prepared plasmid DNA was used to transform E. coli DH10B (Gibco BRL, Burlington, Ontario) by electroporation ( ) to determine if plasmids that could not be transferred by conjugation, and were therefore likely to not be self-transmissible, encoded drugresistance determinants. ..

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: .. The pACYCDuet™-1 vector and its derivatives carrying FL E. coli , M. morganii , and S. gancidicus ScoMcrA homologs with E. coli promoters (pJTU1673, pJTU1674, and pJTU1675) were introduced to E. coli DH10B, and competent cells of the resulting strains were prepared using the standard calcium chloride protocol. .. Transformation frequency was determined by introducing 100 ng pBluescript SK+ (PT− ) or pJTU1238 (PT+ ) plasmid DNA, which carries the dnd gene cluster from Salmonella enterica serovar Cerro 87 , to the competent cells.

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  • 93
    Thermo Fisher e coli dh10b
    SBD-homologous domains might function to recognize PT-DNA. a Multiple sequence alignment of representative ScoMcrA-SBD homologs from Streptomyces coelicolor (#1), Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4). The sulfur-recognizing residues are highlighted in yellow. The consensus sequence is shown at the bottom. Right: Domain organizations of these ScoMcrA homologs. b The surface of ScoMcrA-SBD is colored according to conservation scores, which shows that its sulfur-recognizing cavity is highly conserved. c Purified ScoMcrA-SBD domain homologs from Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4) specifically recognized PT-DNA with the sulfur atom in the R p , but not in the S p , configuration. d Mutation of P482N in the Escherichia coli SBD homolog significantly diminished, while mutation of P607N in the Streptomyces gancidicus SBD homolog disrupted, the association with PT-DNA with the core sequence G PS AAC. e Heterologous expression of scomcrA homologs from S. gancidicus (#2), E. coli (#3), and M. morganii (#4) restricted transfer of the dnd gene cluster from Salmonella enterica , which contains the genes encoding the “writer” proteins of DNA phosphorothioation. Transformation frequencies of empty pBluescript vector (PT − ) and that harboring the dnd gene cluster (PT + ) into E. coli <t>DH10B</t> expressing various scomcrA homologs are shown
    E Coli Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dh10b/product/Thermo Fisher
    Average 93 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    e coli dh10b - by Bioz Stars, 2020-08
    93/100 stars
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    SBD-homologous domains might function to recognize PT-DNA. a Multiple sequence alignment of representative ScoMcrA-SBD homologs from Streptomyces coelicolor (#1), Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4). The sulfur-recognizing residues are highlighted in yellow. The consensus sequence is shown at the bottom. Right: Domain organizations of these ScoMcrA homologs. b The surface of ScoMcrA-SBD is colored according to conservation scores, which shows that its sulfur-recognizing cavity is highly conserved. c Purified ScoMcrA-SBD domain homologs from Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4) specifically recognized PT-DNA with the sulfur atom in the R p , but not in the S p , configuration. d Mutation of P482N in the Escherichia coli SBD homolog significantly diminished, while mutation of P607N in the Streptomyces gancidicus SBD homolog disrupted, the association with PT-DNA with the core sequence G PS AAC. e Heterologous expression of scomcrA homologs from S. gancidicus (#2), E. coli (#3), and M. morganii (#4) restricted transfer of the dnd gene cluster from Salmonella enterica , which contains the genes encoding the “writer” proteins of DNA phosphorothioation. Transformation frequencies of empty pBluescript vector (PT − ) and that harboring the dnd gene cluster (PT + ) into E. coli DH10B expressing various scomcrA homologs are shown

    Journal: Nature Communications

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA

    doi: 10.1038/s41467-018-07093-1

    Figure Lengend Snippet: SBD-homologous domains might function to recognize PT-DNA. a Multiple sequence alignment of representative ScoMcrA-SBD homologs from Streptomyces coelicolor (#1), Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4). The sulfur-recognizing residues are highlighted in yellow. The consensus sequence is shown at the bottom. Right: Domain organizations of these ScoMcrA homologs. b The surface of ScoMcrA-SBD is colored according to conservation scores, which shows that its sulfur-recognizing cavity is highly conserved. c Purified ScoMcrA-SBD domain homologs from Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4) specifically recognized PT-DNA with the sulfur atom in the R p , but not in the S p , configuration. d Mutation of P482N in the Escherichia coli SBD homolog significantly diminished, while mutation of P607N in the Streptomyces gancidicus SBD homolog disrupted, the association with PT-DNA with the core sequence G PS AAC. e Heterologous expression of scomcrA homologs from S. gancidicus (#2), E. coli (#3), and M. morganii (#4) restricted transfer of the dnd gene cluster from Salmonella enterica , which contains the genes encoding the “writer” proteins of DNA phosphorothioation. Transformation frequencies of empty pBluescript vector (PT − ) and that harboring the dnd gene cluster (PT + ) into E. coli DH10B expressing various scomcrA homologs are shown

    Article Snippet: The pACYCDuet™-1 vector and its derivatives carrying FL E. coli , M. morganii , and S. gancidicus ScoMcrA homologs with E. coli promoters (pJTU1673, pJTU1674, and pJTU1675) were introduced to E. coli DH10B, and competent cells of the resulting strains were prepared using the standard calcium chloride protocol.

    Techniques: Sequencing, Purification, Mutagenesis, Expressing, Transformation Assay, Plasmid Preparation

    SBD-homologous domains might function to recognize PT-DNA. a Multiple sequence alignment of representative ScoMcrA-SBD homologs from Streptomyces coelicolor (#1), Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4). The sulfur-recognizing residues are highlighted in yellow. The consensus sequence is shown at the bottom. Right: Domain organizations of these ScoMcrA homologs. b The surface of ScoMcrA-SBD is colored according to conservation scores, which shows that its sulfur-recognizing cavity is highly conserved. c Purified ScoMcrA-SBD domain homologs from Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4) specifically recognized PT-DNA with the sulfur atom in the R p , but not in the S p , configuration. d Mutation of P482N in the Escherichia coli SBD homolog significantly diminished, while mutation of P607N in the Streptomyces gancidicus SBD homolog disrupted, the association with PT-DNA with the core sequence G PS AAC. e Heterologous expression of scomcrA homologs from S. gancidicus (#2), E. coli (#3), and M. morganii (#4) restricted transfer of the dnd gene cluster from Salmonella enterica , which contains the genes encoding the “writer” proteins of DNA phosphorothioation. Transformation frequencies of empty pBluescript vector (PT − ) and that harboring the dnd gene cluster (PT + ) into E. coli DH10B expressing various scomcrA homologs are shown

    Journal: Nature Communications

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA

    doi: 10.1038/s41467-018-07093-1

    Figure Lengend Snippet: SBD-homologous domains might function to recognize PT-DNA. a Multiple sequence alignment of representative ScoMcrA-SBD homologs from Streptomyces coelicolor (#1), Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4). The sulfur-recognizing residues are highlighted in yellow. The consensus sequence is shown at the bottom. Right: Domain organizations of these ScoMcrA homologs. b The surface of ScoMcrA-SBD is colored according to conservation scores, which shows that its sulfur-recognizing cavity is highly conserved. c Purified ScoMcrA-SBD domain homologs from Streptomyces gancidicus (#2), Escherichia coli (#3), and Morganella morganii (#4) specifically recognized PT-DNA with the sulfur atom in the R p , but not in the S p , configuration. d Mutation of P482N in the Escherichia coli SBD homolog significantly diminished, while mutation of P607N in the Streptomyces gancidicus SBD homolog disrupted, the association with PT-DNA with the core sequence G PS AAC. e Heterologous expression of scomcrA homologs from S. gancidicus (#2), E. coli (#3), and M. morganii (#4) restricted transfer of the dnd gene cluster from Salmonella enterica , which contains the genes encoding the “writer” proteins of DNA phosphorothioation. Transformation frequencies of empty pBluescript vector (PT − ) and that harboring the dnd gene cluster (PT + ) into E. coli DH10B expressing various scomcrA homologs are shown

    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required.

    Techniques: Sequencing, Purification, Mutagenesis, Expressing, Transformation Assay, Plasmid Preparation