escherichia coli dh10b  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher escherichia coli dh10b
    Escherichia Coli Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli dh10b/product/Thermo Fisher
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    escherichia coli dh10b - by Bioz Stars, 2020-01
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Genetic and Biochemical Characterization of the F-ATPase Operon from Streptococcus sanguis 10904
    Article Snippet: .. E. coli DH10B (Invitrogen, Carlsbad, Calif.) , GBE180 , or JP17 ( ) was used for all cloning experiments, as indicated. .. E. coli strains were grown on Luria broth (10 g of tryptone [Difco], 5 g of yeast extract [Difco], 10 g of NaCl per liter) at 37°C ( ).

    Article Title: Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy
    Article Snippet: Paragraph title: Bacterial Strains and Cloning ... These vectors were transformed into E. coli DH10B [endA 1 recA 1 galE 15 galK 16 nupG rpsL Δlac X74 Φ80lac ZΔM15 araD 139 Δ(ara ,leu )7697 mcrA Δ(mrr -hsdRMS -mcrBC )] cells (Invitrogen, Carlsbad, CA) as a host strain and exploited for site-directed mutagenesis using Stratagene’s Quik Change mutagenesis kit to provide bla SHV-1 E166A and bla KPC-2 E166A , respectively, as previously described.

    Article Title: Exploring the Role of Residue 228 in Substrate and Inhibitor Recognition by VIM Metallo-β-lactamases
    Article Snippet: Paragraph title: Bacterial Strains, Cloning, and Mutagenesis ... E. coli DH10B (Invitrogen, Carlsbad, CA) was used as a host strain for pBCSK (−) bla VIM-2 and to obtain a mutant library at position 228 by site saturation mutagenesis, using the QuickChange XL Site-directed Mutagenesis Kit (Stratagene).

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: Paragraph title: Cloning experiments and analysis of recombinant plasmids. ... Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies).

    Article Title: Exploring the Role of a Conserved Class A Residue in the ?-Loop of KPC-2 ?-Lactamase
    Article Snippet: .. Lysogeny broth (LB) minimal inhibitory concentration (MICs) determinations for β-lactams were performed on single clones of E. coli DH10B (Invitrogen) harboring pBR322- catI-bla KPC-2 or a variant. ..

    Article Title: Functional Characterization of IS1999, an IS4 Family Element Involved in Mobilization and Expression of ?-Lactam Resistance Genes
    Article Snippet: E. coli DH10B (Life Technologies, Eragny, France) was used as the bacterial host in electroporation experiments. .. The low-copy-number cloning vector pBBR1MCS.3 was used for cloning experiments ( ).

    Centrifugation:

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: Next, 0.1 ml of 5 M NaCl was added, and after incubation of the cell lysate at 4°C for 24 h, cell debris was cleared by centrifugation. .. To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω.

    Amplification:

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. Genes encoding S. coelicolor FL ScoMcrA, the SBD-SRA domain of ScoMcrA (residues 91–442), and the SBD domain of ScoMcrA (residues 91–260) were amplified by high-fidelity PCR with KOD-Plus DNA polymerase (Toyobo) using primer pairs ScoFL-F/ScoFL-R, SBDSRA-F/SBDSRA-R, and SBD-F/SBD-R, respectively.

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. Genes encoding S. coelicolor FL ScoMcrA, the SBD-SRA domain of ScoMcrA (residues 91–442), and the SBD domain of ScoMcrA (residues 91–260) were amplified by high-fidelity PCR with KOD-Plus DNA polymerase (Toyobo) using primer pairs ScoFL-F/ScoFL-R, SBDSRA-F/SBDSRA-R, and SBD-F/SBD-R, respectively.

    Synthesized:

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. The 1380 bp DNA fragment encoding the E. coli ScoMcrA homolog (WP_000199891) along with its promoter was synthesized by GeneScript according to the sequence in NCBI GenBank (4690337–4691716 of CP006584.1).

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. The 1380 bp DNA fragment encoding the E. coli ScoMcrA homolog (WP_000199891) along with its promoter was synthesized by GeneScript according to the sequence in NCBI GenBank (4690337–4691716 of CP006584.1).

    Construct:

    Article Title: Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli
    Article Snippet: The AlaRS gene (alaS; ACB03816) from E. coli DH10B (Invitrogen, Carlsbad, CA, USA) was inserted between the Nde I and Xho I site in pT5C, resulting in pT5C-Ec-alaS. .. The discrepancy between K73 and the codon number is due to the posttranslational removal of the first methionine (Met) in cells [ ]. pKTS-AcKRS1-PylT ( b), which was used for genetic incorporation of AcK, was constructed by inserting a Methanosarcina mazei pyrrolysine tRNA (tRNAPyl ) expression cassette from pTECH-PylT into the Nhe I site of pKTS-AcKRS1, which overexpresses an Nε -acetyl lysyl-tRNA synthetase (AcKRS) in E. coli [ ].

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: .. To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω. .. The transformed bacteria were grown on Luria-Bertani (LB) agar plates containing 12.5 μg of chloramphenicol per ml.

    Article Title: Genetic Circuit Performance under Conditions Relevant for Industrial Bioreactors
    Article Snippet: Replicates of shake flask experiments used the strain constructed at UCSF, designated as DS68637† in this text. .. E. coli DH10B was obtained from Invitrogen (#18297).

    Variant Assay:

    Article Title: Exploring the Role of a Conserved Class A Residue in the ?-Loop of KPC-2 ?-Lactamase
    Article Snippet: .. Lysogeny broth (LB) minimal inhibitory concentration (MICs) determinations for β-lactams were performed on single clones of E. coli DH10B (Invitrogen) harboring pBR322- catI-bla KPC-2 or a variant. ..

    Incubation:

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: Next, 0.1 ml of 5 M NaCl was added, and after incubation of the cell lysate at 4°C for 24 h, cell debris was cleared by centrifugation. .. To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω.

    Cell Culture:

    Article Title: Jungle Express is a versatile repressor system for tight transcriptional control
    Article Snippet: .. Plasmids were maintained in E. coli DH10B (Invitrogen), which was cultured using lysogeny broth (LB) supplemented with 30 (in liquid medium) or 50 (in solid medium) μg ml−1 kanamycin. ..

    Article Title: Jungle Express is a versatile repressor system for tight transcriptional control
    Article Snippet: .. Growth and induction of non-E. coli strains Plasmids were maintained in E. coli DH10B (Invitrogen), which was cultured using lysogeny broth (LB) supplemented with 30 (in liquid medium) or 50 (in solid medium) μg ml−1 kanamycin. ..

    Expressing:

    Article Title: Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli
    Article Snippet: The AlaRS gene (alaS; ACB03816) from E. coli DH10B (Invitrogen, Carlsbad, CA, USA) was inserted between the Nde I and Xho I site in pT5C, resulting in pT5C-Ec-alaS. .. The discrepancy between K73 and the codon number is due to the posttranslational removal of the first methionine (Met) in cells [ ]. pKTS-AcKRS1-PylT ( b), which was used for genetic incorporation of AcK, was constructed by inserting a Methanosarcina mazei pyrrolysine tRNA (tRNAPyl ) expression cassette from pTECH-PylT into the Nhe I site of pKTS-AcKRS1, which overexpresses an Nε -acetyl lysyl-tRNA synthetase (AcKRS) in E. coli [ ].

    Modification:

    Article Title: Genetic and Biochemical Characterization of the F-ATPase Operon from Streptococcus sanguis 10904
    Article Snippet: S. sanguis ATCC 10904 was grown on Todd-Hewitt broth (30 g per liter) (Difco, Detroit, Mich.) for chromosomal DNA isolation and in a modified Todd-Hewitt broth (per liter: 37 g of brain heart infusion, 1 g of Bacto peptone, 2 g of NaCl, 0.4 g of disodium phosphate, 2.5 g of sodium carbonate, 0.5% glucose) when grown in continuous culture in the chemostat. .. E. coli DH10B (Invitrogen, Carlsbad, Calif.) , GBE180 , or JP17 ( ) was used for all cloning experiments, as indicated.

    Transformation Assay:

    Article Title: Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada
    Article Snippet: Paragraph title: Strain transformation ... The prepared plasmid DNA was used to transform E. coli DH10B (Gibco BRL, Burlington, Ontario) by electroporation ( ) to determine if plasmids that could not be transferred by conjugation, and were therefore likely to not be self-transmissible, encoded drugresistance determinants.

    Article Title: Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy
    Article Snippet: .. These vectors were transformed into E. coli DH10B [endA 1 recA 1 galE 15 galK 16 nupG rpsL Δlac X74 Φ80lac ZΔM15 araD 139 Δ(ara ,leu )7697 mcrA Δ(mrr -hsdRMS -mcrBC )] cells (Invitrogen, Carlsbad, CA) as a host strain and exploited for site-directed mutagenesis using Stratagene’s Quik Change mutagenesis kit to provide bla SHV-1 E166A and bla KPC-2 E166A , respectively, as previously described. .. − E. coli DH10B cells carrying the empty pBCSK (−) vector (no bla ) were used as a control.

    Article Title: Exploring the Role of Residue 228 in Substrate and Inhibitor Recognition by VIM Metallo-β-lactamases
    Article Snippet: E. coli DH10B (Invitrogen, Carlsbad, CA) was used as a host strain for pBCSK (−) bla VIM-2 and to obtain a mutant library at position 228 by site saturation mutagenesis, using the QuickChange XL Site-directed Mutagenesis Kit (Stratagene). .. Briefly, primers that capture all 19 variants at MBL position 228 were designed, and mutagenic polymerase chain reactions were conducted using pBCSK (−) bla VIM-2 as a template; they were transformed into E. coli DH10B.

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: .. Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies). ..

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω. .. The transformed bacteria were grown on Luria-Bertani (LB) agar plates containing 12.5 μg of chloramphenicol per ml.

    Conjugation Assay:

    Article Title: Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada
    Article Snippet: .. The prepared plasmid DNA was used to transform E. coli DH10B (Gibco BRL, Burlington, Ontario) by electroporation ( ) to determine if plasmids that could not be transferred by conjugation, and were therefore likely to not be self-transmissible, encoded drugresistance determinants. ..

    Article Title: Jungle Express is a versatile repressor system for tight transcriptional control
    Article Snippet: Plasmids were maintained in E. coli DH10B (Invitrogen), which was cultured using lysogeny broth (LB) supplemented with 30 (in liquid medium) or 50 (in solid medium) μg ml−1 kanamycin. .. Similarly, E. coli strain HB101/pRK2073 (conferring resistance to spectinomycin) facilitated conjugation into P. putida ; chloramphenicol was used to select against the donor and helper strains.

    Article Title: Functional Characterization of IS1999, an IS4 Family Element Involved in Mobilization and Expression of ?-Lactam Resistance Genes
    Article Snippet: E. coli DH10B (Life Technologies, Eragny, France) was used as the bacterial host in electroporation experiments. .. The recombination-deficient strain E. coli DH5α (pOX38-Gen) and the rifampin-resistant E. coli DH10B Rifr were used for transposition and conjugation experiments ( ).

    Article Title: Jungle Express is a versatile repressor system for tight transcriptional control
    Article Snippet: Growth and induction of non-E. coli strains Plasmids were maintained in E. coli DH10B (Invitrogen), which was cultured using lysogeny broth (LB) supplemented with 30 (in liquid medium) or 50 (in solid medium) μg ml−1 kanamycin. .. Similarly, E. coli strain HB101/pRK2073 (conferring resistance to spectinomycin) facilitated conjugation into P. putida ; chloramphenicol was used to select against the donor and helper strains.

    Transfection:

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω. .. For transfection experiments, HSV-BAC DNAs were isolated by the alkaline lysis procedures and further purified by centrifugation in cesium chloride gradients ( ).

    Southern Blot:

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: The recombinants were screened by PCR with primers that correspond to pBeloBAC11 sequences and then verified by Southern blotting. .. To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω.

    Ligation:

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: Cloning resulted from the ligation of either HindIII-, BamHI-, or EcoRI-digested fragments from the genomic DNA from C. gillenii into the HindIII-, BamHI-, or EcoRI-restricted pK19 vector, respectively ( ). .. Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies).

    Low Copy Number:

    Article Title: Functional Characterization of IS1999, an IS4 Family Element Involved in Mobilization and Expression of ?-Lactam Resistance Genes
    Article Snippet: E. coli DH10B (Life Technologies, Eragny, France) was used as the bacterial host in electroporation experiments. .. The low-copy-number cloning vector pBBR1MCS.3 was used for cloning experiments ( ).

    Infection:

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: .. To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω. .. The transformed bacteria were grown on Luria-Bertani (LB) agar plates containing 12.5 μg of chloramphenicol per ml.

    Sequencing:

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. The 1380 bp DNA fragment encoding the E. coli ScoMcrA homolog (WP_000199891) along with its promoter was synthesized by GeneScript according to the sequence in NCBI GenBank (4690337–4691716 of CP006584.1).

    Article Title: Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli
    Article Snippet: Plasmid Constructions The pT5C plasmid originated from pACYC184, in which a tetracycline resistant gene (tet R ) was replaced by the T5/lac promoter, C-terminal His-tagged sequence, the rrnB terminator, and the lacI q gene ( a). .. The AlaRS gene (alaS; ACB03816) from E. coli DH10B (Invitrogen, Carlsbad, CA, USA) was inserted between the Nde I and Xho I site in pT5C, resulting in pT5C-Ec-alaS.

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. The 1380 bp DNA fragment encoding the E. coli ScoMcrA homolog (WP_000199891) along with its promoter was synthesized by GeneScript according to the sequence in NCBI GenBank (4690337–4691716 of CP006584.1).

    Recombinant:

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: .. Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies). ..

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: Paragraph title: Construction of the recombinant HSVs and E. coli strains harboring HSV-BACs. ... To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω.

    DNA Extraction:

    Article Title: Genetic and Biochemical Characterization of the F-ATPase Operon from Streptococcus sanguis 10904
    Article Snippet: S. sanguis ATCC 10904 was grown on Todd-Hewitt broth (30 g per liter) (Difco, Detroit, Mich.) for chromosomal DNA isolation and in a modified Todd-Hewitt broth (per liter: 37 g of brain heart infusion, 1 g of Bacto peptone, 2 g of NaCl, 0.4 g of disodium phosphate, 2.5 g of sodium carbonate, 0.5% glucose) when grown in continuous culture in the chemostat. .. E. coli DH10B (Invitrogen, Carlsbad, Calif.) , GBE180 , or JP17 ( ) was used for all cloning experiments, as indicated.

    Molecular Cloning:

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: Paragraph title: Molecular cloning ... E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required.

    Electroporation:

    Article Title: Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada
    Article Snippet: .. The prepared plasmid DNA was used to transform E. coli DH10B (Gibco BRL, Burlington, Ontario) by electroporation ( ) to determine if plasmids that could not be transferred by conjugation, and were therefore likely to not be self-transmissible, encoded drugresistance determinants. ..

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: .. Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies). ..

    Article Title: Functional Characterization of IS1999, an IS4 Family Element Involved in Mobilization and Expression of ?-Lactam Resistance Genes
    Article Snippet: .. E. coli DH10B (Life Technologies, Eragny, France) was used as the bacterial host in electroporation experiments. .. The recombination-deficient strain E. coli DH5α (pOX38-Gen) and the rifampin-resistant E. coli DH10B Rifr were used for transposition and conjugation experiments ( ).

    Mutagenesis:

    Article Title: Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli
    Article Snippet: The AlaRS gene (alaS; ACB03816) from E. coli DH10B (Invitrogen, Carlsbad, CA, USA) was inserted between the Nde I and Xho I site in pT5C, resulting in pT5C-Ec-alaS. .. The lysine codon (AAA) at position 74 of the alaS gene on pT5C-Ec-alaS was substituted to an alanine (GCG) or amber stop (TAG) codon by site-directed mutagenesis, resulting in pT5C-Ec-alaS-K74A and pT5C-Ec-alaS-K74amb, respectively.

    Article Title: Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy
    Article Snippet: .. These vectors were transformed into E. coli DH10B [endA 1 recA 1 galE 15 galK 16 nupG rpsL Δlac X74 Φ80lac ZΔM15 araD 139 Δ(ara ,leu )7697 mcrA Δ(mrr -hsdRMS -mcrBC )] cells (Invitrogen, Carlsbad, CA) as a host strain and exploited for site-directed mutagenesis using Stratagene’s Quik Change mutagenesis kit to provide bla SHV-1 E166A and bla KPC-2 E166A , respectively, as previously described. .. − E. coli DH10B cells carrying the empty pBCSK (−) vector (no bla ) were used as a control.

    Article Title: Exploring the Role of Residue 228 in Substrate and Inhibitor Recognition by VIM Metallo-β-lactamases
    Article Snippet: .. E. coli DH10B (Invitrogen, Carlsbad, CA) was used as a host strain for pBCSK (−) bla VIM-2 and to obtain a mutant library at position 228 by site saturation mutagenesis, using the QuickChange XL Site-directed Mutagenesis Kit (Stratagene). .. Briefly, primers that capture all 19 variants at MBL position 228 were designed, and mutagenic polymerase chain reactions were conducted using pBCSK (−) bla VIM-2 as a template; they were transformed into E. coli DH10B.

    Isolation:

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: Circular viral DNA of YK301 was isolated from infected Vero or rabbit skin cells by the Hirt method ( ). .. To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω.

    Purification:

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω. .. For transfection experiments, HSV-BAC DNAs were isolated by the alkaline lysis procedures and further purified by centrifugation in cesium chloride gradients ( ).

    Polymerase Chain Reaction:

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. Genes encoding S. coelicolor FL ScoMcrA, the SBD-SRA domain of ScoMcrA (residues 91–442), and the SBD domain of ScoMcrA (residues 91–260) were amplified by high-fidelity PCR with KOD-Plus DNA polymerase (Toyobo) using primer pairs ScoFL-F/ScoFL-R, SBDSRA-F/SBDSRA-R, and SBD-F/SBD-R, respectively.

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. Genes encoding S. coelicolor FL ScoMcrA, the SBD-SRA domain of ScoMcrA (residues 91–442), and the SBD domain of ScoMcrA (residues 91–260) were amplified by high-fidelity PCR with KOD-Plus DNA polymerase (Toyobo) using primer pairs ScoFL-F/ScoFL-R, SBDSRA-F/SBDSRA-R, and SBD-F/SBD-R, respectively.

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: The recombinants were screened by PCR with primers that correspond to pBeloBAC11 sequences and then verified by Southern blotting. .. To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω.

    Selection:

    Article Title: Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
    Article Snippet: E. coli DH10B was purchased from Invitrogen. .. For sacB counter selection, we used LBNS plates ( LB n o s alt: 1% Tryptone, 0.5% yeast extract and 1.5% agar) supplemented with 10% sucrose (Fisher Scientific).

    Plasmid Preparation:

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. The PCR products were cleaved by BglII/EcoRI or BamHI/EcoRI (New England Biolab) and inserted into the pSJ8 plasmid using T4 DNA ligase (Takara), resulting in pJTU1660, pJTU1668, and pJTU1669.

    Article Title: Characterization of antimicrobial resistance of Salmonella Newport isolated from animals, the environment, and animal food products in Canada
    Article Snippet: .. The prepared plasmid DNA was used to transform E. coli DH10B (Gibco BRL, Burlington, Ontario) by electroporation ( ) to determine if plasmids that could not be transferred by conjugation, and were therefore likely to not be self-transmissible, encoded drugresistance determinants. ..

    Article Title: Lysine Acetylation Regulates Alanyl-tRNA Synthetase Activity in Escherichia coli
    Article Snippet: Paragraph title: 2.2. Plasmid Constructions ... The AlaRS gene (alaS; ACB03816) from E. coli DH10B (Invitrogen, Carlsbad, CA, USA) was inserted between the Nde I and Xho I site in pT5C, resulting in pT5C-Ec-alaS.

    Article Title: Following Drug Uptake and Reactions inside Escherichia coli Cells by Raman Microspectroscopy
    Article Snippet: These vectors were transformed into E. coli DH10B [endA 1 recA 1 galE 15 galK 16 nupG rpsL Δlac X74 Φ80lac ZΔM15 araD 139 Δ(ara ,leu )7697 mcrA Δ(mrr -hsdRMS -mcrBC )] cells (Invitrogen, Carlsbad, CA) as a host strain and exploited for site-directed mutagenesis using Stratagene’s Quik Change mutagenesis kit to provide bla SHV-1 E166A and bla KPC-2 E166A , respectively, as previously described. .. − E. coli DH10B cells carrying the empty pBCSK (−) vector (no bla ) were used as a control.

    Article Title: Exploring the Role of Residue 228 in Substrate and Inhibitor Recognition by VIM Metallo-β-lactamases
    Article Snippet: A forward primer containing the SacI restriction site (5′-AAA GAGCTC AAG AAG GAG ATA TAC ATA TG-3′) and a reverse primer with the BamHI restriction site (5′-CTC AGT CGT TGA GTA G GGATCC -3′) were used to amplify bla VIM-2 , including the pET24a(+) ribosomal binding site, using plasmid pET-24a(+)- bla VIM-2 as a template. .. E. coli DH10B (Invitrogen, Carlsbad, CA) was used as a host strain for pBCSK (−) bla VIM-2 and to obtain a mutant library at position 228 by site saturation mutagenesis, using the QuickChange XL Site-directed Mutagenesis Kit (Stratagene).

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: Cloning resulted from the ligation of either HindIII-, BamHI-, or EcoRI-digested fragments from the genomic DNA from C. gillenii into the HindIII-, BamHI-, or EcoRI-restricted pK19 vector, respectively ( ). .. Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies).

    Article Title: Structural basis for the recognition of sulfur in phosphorothioated DNA
    Article Snippet: E. coli DH10B (Thermo Fisher) and Escherichiacoli BL21(DE3) (Novagen) was grown in Luria Broth medium supplemented with 100 mg/mL ampicillin or 34 mg/mL chloramphenicol as required. .. The PCR products were cleaved by BglII/EcoRI or BamHI/EcoRI (New England Biolab) and inserted into the pSJ8 plasmid using T4 DNA ligase (Takara), resulting in pJTU1660, pJTU1668, and pJTU1669.

    Article Title: Functional Characterization of IS1999, an IS4 Family Element Involved in Mobilization and Expression of ?-Lactam Resistance Genes
    Article Snippet: Clinical strains P. aeruginosa 1 ( bla VEB-1 ), K. pneumoniae 11978 ( bla OXA-48 ), and Escherichia coli DH10B (pA-1) harboring the natural plasmid of K. pneumoniae 11978 have been previously described ( , ). .. E. coli DH10B (Life Technologies, Eragny, France) was used as the bacterial host in electroporation experiments.

    Article Title: Genetic Circuit Performance under Conditions Relevant for Industrial Bioreactors
    Article Snippet: KanR was then removed using the FRT recombinase encoded by the plasmid pCP20. .. E. coli DH10B was obtained from Invitrogen (#18297).

    Binding Assay:

    Article Title: Exploring the Role of Residue 228 in Substrate and Inhibitor Recognition by VIM Metallo-β-lactamases
    Article Snippet: A forward primer containing the SacI restriction site (5′-AAA GAGCTC AAG AAG GAG ATA TAC ATA TG-3′) and a reverse primer with the BamHI restriction site (5′-CTC AGT CGT TGA GTA G GGATCC -3′) were used to amplify bla VIM-2 , including the pET24a(+) ribosomal binding site, using plasmid pET-24a(+)- bla VIM-2 as a template. .. E. coli DH10B (Invitrogen, Carlsbad, CA) was used as a host strain for pBCSK (−) bla VIM-2 and to obtain a mutant library at position 228 by site saturation mutagenesis, using the QuickChange XL Site-directed Mutagenesis Kit (Stratagene).

    Agarose Gel Electrophoresis:

    Article Title: Chromosome-Encoded Narrow-Spectrum Ambler Class A ?-Lactamase GIL-1 from Citrobacter gillenii ▿
    Article Snippet: Recombinant plasmids were transformed by electroporation (Gene Pulser II; Bio-Rad, Ivry-sur-Seine, France) into E. coli DH10B (Life Technologies). .. Recombinant plasmid DNAs were extracted with a plasmid DNA maxi kit (QIAGEN, Courtaboeuf, France) and analyzed by restriction endonuclease digestions (Amersham Biosciences) and agarose gel electrophoresis (Life Technologies).

    Concentration Assay:

    Article Title: Genetic and Biochemical Characterization of the F-ATPase Operon from Streptococcus sanguis 10904
    Article Snippet: E. coli DH10B (Invitrogen, Carlsbad, Calif.) , GBE180 , or JP17 ( ) was used for all cloning experiments, as indicated. .. 5-Bromo-4-chloro-3-indolyl-β- d -galactosidase (X-Gal) was used at a concentration of 20 mg/ml in solid medium to facilitate screening of prospective clones.

    Article Title: Exploring the Role of a Conserved Class A Residue in the ?-Loop of KPC-2 ?-Lactamase
    Article Snippet: .. Lysogeny broth (LB) minimal inhibitory concentration (MICs) determinations for β-lactams were performed on single clones of E. coli DH10B (Invitrogen) harboring pBR322- catI-bla KPC-2 or a variant. ..

    Alkaline Lysis:

    Article Title: Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo
    Article Snippet: To construct E. coli YEbac101, which harbors circular viral DNA of YK301, the DNA from infected cells was electroporated into E. coli DH10B (Invitrogen), using a Bio-Rad Gene Pulser II with 0.1-cm cuvettes at 1.8 kV, 25 μF, and 200 Ω. .. HSV-BAC DNAs were isolated from the E. coli cultures of chloramphenicol-resistant colonies by standard alkaline lysis procedures ( ) and screened by PCR with appropriate primer sets or restriction enzyme digestion.

    Positron Emission Tomography:

    Article Title: Exploring the Role of Residue 228 in Substrate and Inhibitor Recognition by VIM Metallo-β-lactamases
    Article Snippet: A forward primer containing the SacI restriction site (5′-AAA GAGCTC AAG AAG GAG ATA TAC ATA TG-3′) and a reverse primer with the BamHI restriction site (5′-CTC AGT CGT TGA GTA G GGATCC -3′) were used to amplify bla VIM-2 , including the pET24a(+) ribosomal binding site, using plasmid pET-24a(+)- bla VIM-2 as a template. .. E. coli DH10B (Invitrogen, Carlsbad, CA) was used as a host strain for pBCSK (−) bla VIM-2 and to obtain a mutant library at position 228 by site saturation mutagenesis, using the QuickChange XL Site-directed Mutagenesis Kit (Stratagene).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher megax dh10b t1r electrocomp cells
    Megax Dh10b T1r Electrocomp Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megax dh10b t1r electrocomp cells/product/Thermo Fisher
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    megax dh10b t1r electrocomp cells - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher max efficiency dh10b competent cells
    Max Efficiency Dh10b Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/max efficiency dh10b competent cells/product/Thermo Fisher
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    max efficiency dh10b competent cells - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    N/A
    The E coli DH10b Control 600 Library provides a templating control for use with the Ion 520 Ion 530 ExT Kit Chef and the Ion S5 and Ion S5 XL
      Buy from Supplier

    Image Search Results