escherichia coli cultures  (Thermo Fisher)


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    Structured Review

    Thermo Fisher escherichia coli cultures
    Escherichia Coli Cultures, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli cultures/product/Thermo Fisher
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli cultures - by Bioz Stars, 2020-08
    89/100 stars

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    Related Articles

    Isolation:

    Article Title: Rapid and assured genetic engineering methods applied to Acinetobacter baylyi ADP1 genome streamlining
    Article Snippet: .. Plasmid samples input into GGA reactions were isolated from Escherichia coli cultures using the PureLink Quick Plasmid Miniprep Kit (ThermoFisher). .. Input PCR products were purified using the GeneJet PCR purification kit (ThermoFisher).

    other:

    Article Title: The NanI and NanJ Sialidases of Clostridium perfringens Are Not Essential for Virulence
    Article Snippet: The Escherichia coli cultures were derivatives of DH5α (Life Technologies) or NovaBlue (Novagen) and were propagated in 2× YT medium ( ) supplemented with ampicillin (100 μg/ml), chloramphenicol (30 μg/ml), erythromycin (150 μg/ml), or tetracycline (10 μg/ml).

    Article Title: Deacetylation of sialic acid by esterases potentiates pneumococcal neuraminidase activity for mucin utilization, colonization and virulence
    Article Snippet: Escherichia coli cultures were grown on Luria broth, or Luria agar (Oxoid, UK).

    Plasmid Preparation:

    Article Title: Rapid and assured genetic engineering methods applied to Acinetobacter baylyi ADP1 genome streamlining
    Article Snippet: .. Plasmid samples input into GGA reactions were isolated from Escherichia coli cultures using the PureLink Quick Plasmid Miniprep Kit (ThermoFisher). .. Input PCR products were purified using the GeneJet PCR purification kit (ThermoFisher).

    Spectrophotometry:

    Article Title: Inhibition of Escherichia coli Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ †
    Article Snippet: .. An overnight E. coli culture grown in LB medium with 100 μg/ml ampicillin was used to inoculate biofilm cultures in the same medium supplemented with 6 g/liter arabinose to an OD600 of 0.05 as measured with a Genesis 5 spectrophotometer (Spectronic Instruments, Rochester, NY). ..

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  • 91
    Thermo Fisher environmental microalgae
    Taxonomic assignments of environmental microbial SAGs. The following approaches were used: PCR-based sequencing of SSU rRNA genes followed by classification with CREST (prokaryotes) or Silva Incremental Aligner <t>(microalgae);</t> LoCoS followed by CheckM, Metaxa, and CREST; and a combination of the two approaches. 317 SAGs from each environment, cell type, and gDNA amplification method were analyzed
    Environmental Microalgae, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/environmental microalgae/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    environmental microalgae - by Bioz Stars, 2020-08
    91/100 stars
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    89
    Thermo Fisher top10 escherichia coli
    DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into <t>TOP10</t> <t>Escherichia</t> coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).
    Top10 Escherichia Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/top10 escherichia coli/product/Thermo Fisher
    Average 89 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    top10 escherichia coli - by Bioz Stars, 2020-08
    89/100 stars
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    91
    Thermo Fisher phrodo green e coli bioparticles
    LPS treatment during BMDM differentiation reduced phagocytosis. BMDM treated on day 2 or day 7 with LPS (250 ng/mL for 16 h) were then cultured with <t>pHrodo-labeled</t> E. coli <t>BioParticles.</t> Phagocytosis (endocytosis and intravesicular acidification) was measured
    Phrodo Green E Coli Bioparticles, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phrodo green e coli bioparticles/product/Thermo Fisher
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    phrodo green e coli bioparticles - by Bioz Stars, 2020-08
    91/100 stars
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    85
    Thermo Fisher o157 serotyping
    Confirmation tests on newly converted STEC. (A) Representative picture of in situ colony hybridization with DIG labeled stx 2A probe; (B) PCR confirmation of stx 2A gene in newly converted STEC strains (PS1–PS3); (C) ELISA detection of Shiga toxins; (D) <t>O157</t> <t>serotyping</t> of newly converted STEC; (E) PCR confirmation of O157 gene was absent in newly converted STEC strains (PS1–PS3).
    O157 Serotyping, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    o157 serotyping - by Bioz Stars, 2020-08
    85/100 stars
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    Image Search Results


    Taxonomic assignments of environmental microbial SAGs. The following approaches were used: PCR-based sequencing of SSU rRNA genes followed by classification with CREST (prokaryotes) or Silva Incremental Aligner (microalgae); LoCoS followed by CheckM, Metaxa, and CREST; and a combination of the two approaches. 317 SAGs from each environment, cell type, and gDNA amplification method were analyzed

    Journal: Nature Communications

    Article Title: Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

    doi: 10.1038/s41467-017-00128-z

    Figure Lengend Snippet: Taxonomic assignments of environmental microbial SAGs. The following approaches were used: PCR-based sequencing of SSU rRNA genes followed by classification with CREST (prokaryotes) or Silva Incremental Aligner (microalgae); LoCoS followed by CheckM, Metaxa, and CREST; and a combination of the two approaches. 317 SAGs from each environment, cell type, and gDNA amplification method were analyzed

    Article Snippet: Apart from cultured and environmental microalgae, all other samples were incubated with the SYTO-9 DNA stain (5 μM; Thermo Fisher Scientific) for 10–60 min. FACS was performed using a BD InFlux Mariner flow cytometer equipped with a 488 nm laser for excitation and either a 70 μm (for bacteria and extracellular particles) or 100 μm (for eukaryotes) nozzle orifice (Becton Dickinson, formerly Cytopeia).

    Techniques: Polymerase Chain Reaction, Sequencing, Amplification

    DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into TOP10 Escherichia coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).

    Journal: Clinical and Experimental Immunology

    Article Title: Tissue‐resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer

    doi: 10.1111/cei.13183

    Figure Lengend Snippet: DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into TOP10 Escherichia coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).

    Article Snippet: Next, the PCR amplicons were TA‐cloned into pCR4‐TOPO vector (ThermoFisher) and transformed into TOP10 Escherichia coli (ThermoFisher) by heat shock, according to the manufacturer’s protocol.

    Techniques: DNA Methylation Assay, Sequencing, Cell Culture, Recombinant, In Vitro, Concentration Assay, Incubation, Flow Cytometry, Cytometry, Expressing, Clone Assay, Plasmid Preparation, Transformation Assay

    LPS treatment during BMDM differentiation reduced phagocytosis. BMDM treated on day 2 or day 7 with LPS (250 ng/mL for 16 h) were then cultured with pHrodo-labeled E. coli BioParticles. Phagocytosis (endocytosis and intravesicular acidification) was measured

    Journal: Journal of Innate Immunity

    Article Title: TLR Activation Alters Bone Marrow-Derived Macrophage Differentiation

    doi: 10.1159/000494070

    Figure Lengend Snippet: LPS treatment during BMDM differentiation reduced phagocytosis. BMDM treated on day 2 or day 7 with LPS (250 ng/mL for 16 h) were then cultured with pHrodo-labeled E. coli BioParticles. Phagocytosis (endocytosis and intravesicular acidification) was measured

    Article Snippet: Phagocytosis assays were performed using pHrodo Green E. coli BioParticles (Thermo Fisher Scientific).

    Techniques: Cell Culture, Labeling

    Confirmation tests on newly converted STEC. (A) Representative picture of in situ colony hybridization with DIG labeled stx 2A probe; (B) PCR confirmation of stx 2A gene in newly converted STEC strains (PS1–PS3); (C) ELISA detection of Shiga toxins; (D) O157 serotyping of newly converted STEC; (E) PCR confirmation of O157 gene was absent in newly converted STEC strains (PS1–PS3).

    Journal: PLoS ONE

    Article Title: High Temperature in Combination with UV Irradiation Enhances Horizontal Transfer of stx2 Gene from E. coli O157:H7 to Non-Pathogenic E. coli

    doi: 10.1371/journal.pone.0031308

    Figure Lengend Snippet: Confirmation tests on newly converted STEC. (A) Representative picture of in situ colony hybridization with DIG labeled stx 2A probe; (B) PCR confirmation of stx 2A gene in newly converted STEC strains (PS1–PS3); (C) ELISA detection of Shiga toxins; (D) O157 serotyping of newly converted STEC; (E) PCR confirmation of O157 gene was absent in newly converted STEC strains (PS1–PS3).

    Article Snippet: Overnight cultures were boiled at 100°C for 30 min then used for O157 serotyping using an E. coli O157:H7 Latex test kit according to the manual instruction (RIM E. coli O157:H7 Latex test, Thermo Fisher Scientific).

    Techniques: In Situ, Hybridization, Labeling, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay