escherichia coli cultures  (Thermo Fisher)


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    Name:
    GeneArt Stab Agar Culture E coli
    Description:
    The GeneArt Gene Synthesis service offers chemical synthesis cloning and sequence verification of virtually any desired genetic sequence
    Catalog Number:
    889060DE
    Price:
    None
    Category:
    DNA Vectors Clones Purified Nucleic Acids Libraries
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    Name:
    GeneArt Glycerol Stock Culture E coli
    Description:
    The GeneArt Gene Synthesis service offers chemical synthesis cloning and sequence verification of virtually any desired genetic sequence
    Catalog Number:
    889040DE
    Price:
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    Category:
    DNA Vectors Clones Purified Nucleic Acids Libraries
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    Structured Review

    Thermo Fisher escherichia coli cultures
    The GeneArt Gene Synthesis service offers chemical synthesis cloning and sequence verification of virtually any desired genetic sequence
    https://www.bioz.com/result/escherichia coli cultures/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli cultures - by Bioz Stars, 2020-01
    79/100 stars

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    Binding Assay:

    Article Title: Development of an Indirect ELISA Based on a Recombinant Chimeric Protein for the Detection of Antibodies against Bovine Babesiosis
    Article Snippet: Pre-Adsorption of Serum Samples Cattle sera were incubated at 37 °C for 2 h in pre-adsorption solution to avoid unspecific binding of bovine antibodies [ ]. .. Briefly, the bacterial lysate was obtained from 50 mL of overnight Escherichia coli culture of strain BL21 AI™ (Invitrogen).

    Sample Prep:

    Article Title: Inhibition of Escherichia coli Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ †
    Article Snippet: An overnight E. coli culture grown in LB medium with 100 μg/ml ampicillin was used to inoculate biofilm cultures in the same medium supplemented with 6 g/liter arabinose to an OD600 of 0.05 as measured with a Genesis 5 spectrophotometer (Spectronic Instruments, Rochester, NY). .. To minimize the variation in sample preparation, different SAM-modified surfaces were incubated in the same petri dish containing 20 ml medium.

    Mutagenesis:

    Article Title: Disruption of the BMEI0066 gene attenuates the virulence of Brucella melitensis and decreases its stress tolerance
    Article Snippet: Escherichia coli cultures were routinely grown on Luria-Bertani (Oxoid) plates overnight at 37°C with or without supplemental kanamycin (100 mg/liter), ampicillin (100 mg/liter), or chloramphenicol (30 mg/liter). .. This strain was used as a virulent control organism and to generate mutants via deletion mutagenesis.

    Cell Culture:

    Article Title: Inhibition of Escherichia coli Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ †
    Article Snippet: An overnight E. coli culture grown in LB medium with 100 μg/ml ampicillin was used to inoculate biofilm cultures in the same medium supplemented with 6 g/liter arabinose to an OD600 of 0.05 as measured with a Genesis 5 spectrophotometer (Spectronic Instruments, Rochester, NY). .. Biofilms were cultured in plastic petri dishes (100 by 15 mm) at 37°C without shaking.

    Incubation:

    Article Title: Inhibition of Escherichia coli Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ †
    Article Snippet: An overnight E. coli culture grown in LB medium with 100 μg/ml ampicillin was used to inoculate biofilm cultures in the same medium supplemented with 6 g/liter arabinose to an OD600 of 0.05 as measured with a Genesis 5 spectrophotometer (Spectronic Instruments, Rochester, NY). .. To minimize the variation in sample preparation, different SAM-modified surfaces were incubated in the same petri dish containing 20 ml medium.

    Article Title: Development of an Indirect ELISA Based on a Recombinant Chimeric Protein for the Detection of Antibodies against Bovine Babesiosis
    Article Snippet: Pre-Adsorption of Serum Samples Cattle sera were incubated at 37 °C for 2 h in pre-adsorption solution to avoid unspecific binding of bovine antibodies [ ]. .. Briefly, the bacterial lysate was obtained from 50 mL of overnight Escherichia coli culture of strain BL21 AI™ (Invitrogen).

    Article Title: Efficacy of a Lactococcus lactis ΔpyrG vaccine delivery platform expressing chromosomally integrated hly from Listeria monocytogenes
    Article Snippet: Luria-Bertani (LB) broth was used for Escherichia coli cultures while M17 broth (Oxoid) supplemented with 0.5% glucose (i.e., GM17) was used for Lactococcus. .. Incubation temperatures were 30°C for L. lactis and 37°C for L. monocytogenes and E. coli respectively unless otherwise stated.

    other:

    Article Title: The NanI and NanJ Sialidases of Clostridium perfringens Are Not Essential for Virulence
    Article Snippet: The Escherichia coli cultures were derivatives of DH5α (Life Technologies) or NovaBlue (Novagen) and were propagated in 2× YT medium ( ) supplemented with ampicillin (100 μg/ml), chloramphenicol (30 μg/ml), erythromycin (150 μg/ml), or tetracycline (10 μg/ml).

    Article Title: Deacetylation of sialic acid by esterases potentiates pneumococcal neuraminidase activity for mucin utilization, colonization and virulence
    Article Snippet: Escherichia coli cultures were grown on Luria broth, or Luria agar (Oxoid, UK).

    Infection:

    Article Title: Efficient and Reliable Production of Vectors for the Study of the Repair, Mutagenesis, and Phenotypic Consequences of Defined DNA Damage Lesions in Mammalian Cells
    Article Snippet: Escherichia coli Culture and M13KO7 Phage DH12S E . coli cells (Invitrogen Cat. #18312–017), which are endA+ in order to mimimize dsDNA production, were transformed with pcDNA3.2(+) plasmids containing the insert of interest and grown in LB-Miller medium containing 100 μg/mL carbenicillin at 37°C and 225 rpm. .. On the day of phage infection, cultures were diluted 1:500 in 2X-YT medium with carbenicillin.

    BIA-KA:

    Article Title: Development of an Indirect ELISA Based on a Recombinant Chimeric Protein for the Detection of Antibodies against Bovine Babesiosis
    Article Snippet: Briefly, the bacterial lysate was obtained from 50 mL of overnight Escherichia coli culture of strain BL21 AI™ (Invitrogen). .. Total protein was quantified with a BCA commercial kit (Pierce, Rockford, IL, USA) and stored at −20 °C until used.

    Lysis:

    Article Title: Development of an Indirect ELISA Based on a Recombinant Chimeric Protein for the Detection of Antibodies against Bovine Babesiosis
    Article Snippet: Briefly, the bacterial lysate was obtained from 50 mL of overnight Escherichia coli culture of strain BL21 AI™ (Invitrogen). .. The supernatant was discarded and the pellet resuspended with gentle agitation for 2 h at 4 °C in 4 mL of lysis buffer (100 mM Tris HCl, pH: 7.5; 500 mM NaCl; 20% glycerol; 1% Triton X-100; 20 mM imidazole (pH: 7.4); plus 1 mg/mL Lysozyme and 0.5 mM phenylmethylsulfonyl fluoride).

    Transformation Assay:

    Article Title: Gluten quality of bread wheat is associated with activity of RabD GTPases
    Article Snippet: .. Plasmid miniprep Plasmid was recovered from transformed Escherichia coli culture using a Fermentas GeneJET™ plasmid miniprep kit (Fermentas GmbH, St. Leon-Rot, Germany) following the manufacturer's instructions. .. E. coli transformation and culture Competent DH5-α E. coli cells were transformed with pHMW-Adh-Nos vector using the method described by Sambrook et al . ( ).

    Article Title: Efficient and Reliable Production of Vectors for the Study of the Repair, Mutagenesis, and Phenotypic Consequences of Defined DNA Damage Lesions in Mammalian Cells
    Article Snippet: .. Escherichia coli Culture and M13KO7 Phage DH12S E . coli cells (Invitrogen Cat. #18312–017), which are endA+ in order to mimimize dsDNA production, were transformed with pcDNA3.2(+) plasmids containing the insert of interest and grown in LB-Miller medium containing 100 μg/mL carbenicillin at 37°C and 225 rpm. .. On the day of phage infection, cultures were diluted 1:500 in 2X-YT medium with carbenicillin.

    Spectrophotometry:

    Article Title: Inhibition of Escherichia coli Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ Biofilm Formation by Self-Assembled Monolayers of Functional Alkanethiols on Gold ▿ †
    Article Snippet: .. An overnight E. coli culture grown in LB medium with 100 μg/ml ampicillin was used to inoculate biofilm cultures in the same medium supplemented with 6 g/liter arabinose to an OD600 of 0.05 as measured with a Genesis 5 spectrophotometer (Spectronic Instruments, Rochester, NY). ..

    Plasmid Preparation:

    Article Title: Gluten quality of bread wheat is associated with activity of RabD GTPases
    Article Snippet: .. Plasmid miniprep Plasmid was recovered from transformed Escherichia coli culture using a Fermentas GeneJET™ plasmid miniprep kit (Fermentas GmbH, St. Leon-Rot, Germany) following the manufacturer's instructions. .. E. coli transformation and culture Competent DH5-α E. coli cells were transformed with pHMW-Adh-Nos vector using the method described by Sambrook et al . ( ).

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    These TaqMan assay beads provide a simple reliable and rapid method for detecting the presence of a specific bacterial or viral target The assay uses PCR to amplify a DNA
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    77
    Thermo Fisher e coli liquid culture filtrates
    s produced by R. fascians mt s and fas4 isolated from <t>filtrates</t> of <t>E.</t> <t>coli</t> <t>cultures</t> expressing R. fascians fas4mt2 and fas4mt1mt2 , 1 -d 6 , 1 -d 6 (bottom) were analyzed with a hybrid quadrupole-Orbitrap mass spectrometer in the positive ion mode. Predicted fragmented structures are shown above the peaks.
    E Coli Liquid Culture Filtrates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli liquid culture filtrates/product/Thermo Fisher
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    e coli liquid culture filtrates - by Bioz Stars, 2020-01
    77/100 stars
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    88
    Thermo Fisher culture conditions escherichia coli top10
    s produced by R. fascians mt s and fas4 isolated from <t>filtrates</t> of <t>E.</t> <t>coli</t> <t>cultures</t> expressing R. fascians fas4mt2 and fas4mt1mt2 , 1 -d 6 , 1 -d 6 (bottom) were analyzed with a hybrid quadrupole-Orbitrap mass spectrometer in the positive ion mode. Predicted fragmented structures are shown above the peaks.
    Culture Conditions Escherichia Coli Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/culture conditions escherichia coli top10/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    culture conditions escherichia coli top10 - by Bioz Stars, 2020-01
    88/100 stars
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    82
    Thermo Fisher ehec culture
    s produced by R. fascians mt s and fas4 isolated from <t>filtrates</t> of <t>E.</t> <t>coli</t> <t>cultures</t> expressing R. fascians fas4mt2 and fas4mt1mt2 , 1 -d 6 , 1 -d 6 (bottom) were analyzed with a hybrid quadrupole-Orbitrap mass spectrometer in the positive ion mode. Predicted fragmented structures are shown above the peaks.
    Ehec Culture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ehec culture/product/Thermo Fisher
    Average 82 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ehec culture - by Bioz Stars, 2020-01
    82/100 stars
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    Image Search Results


    s produced by R. fascians mt s and fas4 isolated from filtrates of E. coli cultures expressing R. fascians fas4mt2 and fas4mt1mt2 , 1 -d 6 , 1 -d 6 (bottom) were analyzed with a hybrid quadrupole-Orbitrap mass spectrometer in the positive ion mode. Predicted fragmented structures are shown above the peaks.

    Journal: Plant Physiology

    Article Title: Methylated Cytokinins from the Phytopathogen Rhodococcus fascians Mimic Plant Hormone Activity

    doi: 10.1104/pp.15.00787

    Figure Lengend Snippet: s produced by R. fascians mt s and fas4 isolated from filtrates of E. coli cultures expressing R. fascians fas4mt2 and fas4mt1mt2 , 1 -d 6 , 1 -d 6 (bottom) were analyzed with a hybrid quadrupole-Orbitrap mass spectrometer in the positive ion mode. Predicted fragmented structures are shown above the peaks.

    Article Snippet: Purified s from E. coli liquid culture filtrates were analyzed by Q-Exactive (Thermo Scientific), a hybrid quadrupole-Orbitrap mass spectrometer in the positive ion mode, and fragmented structures were predicted with Mass Frontier (Thermo Scientific).

    Techniques: Produced, Isolation, Expressing, Mass Spectrometry