ehec o157 h7 edl933 strain atcc 700927  (ATCC)


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    ATCC ehec o157 h7 edl933 strain atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Ehec O157 H7 Edl933 Strain Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli"

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-12-231

    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Labeling

    Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Plasmid Preparation, Labeling

    SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Techniques Used: Mutagenesis, Western Blot, SDS Page

    SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Labeling

    SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.
    Figure Legend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Techniques Used: Infection, Mutagenesis

    escherichia coli atcc 700927  (ATCC)


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    Structured Review

    ATCC escherichia coli atcc 700927
    Sucrose-based autotroph-heterotroph co-cultures and their products.
    Escherichia Coli Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    Images

    1) Product Images from "Cyanobacteria as cell factories for the photosynthetic production of sucrose"

    Article Title: Cyanobacteria as cell factories for the photosynthetic production of sucrose

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2023.1126032


    Figure Legend Snippet: Sucrose-based autotroph-heterotroph co-cultures and their products.

    Techniques Used:


    Figure Legend Snippet: Synthetic cyanobacteria-heterotroph microbial consortia used as a platform to study microbial interactions.

    Techniques Used:

    ehec o157 h7 edl933 strain atcc 700927  (ATCC)


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    Structured Review

    ATCC ehec o157 h7 edl933 strain atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Ehec O157 H7 Edl933 Strain Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ehec o157 h7 edl933 strain atcc 700927/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ehec o157 h7 edl933 strain atcc 700927 - by Bioz Stars, 2024-02
    95/100 stars

    Images

    1) Product Images from "SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli"

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-12-231

    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Labeling

    Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Plasmid Preparation, Labeling

    SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Techniques Used: Mutagenesis, Western Blot, SDS Page

    SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Labeling

    SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.
    Figure Legend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Techniques Used: Infection, Mutagenesis

    edl933 atcc 700927  (ATCC)


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    Structured Review

    ATCC edl933 atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Edl933 Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edl933 atcc 700927/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edl933 atcc 700927 - by Bioz Stars, 2024-02
    95/100 stars

    Images

    1) Product Images from "SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli"

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-12-231

    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Labeling

    Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Plasmid Preparation, Labeling

    SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Techniques Used: Mutagenesis, Western Blot, SDS Page

    SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Labeling

    SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.
    Figure Legend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Techniques Used: Infection, Mutagenesis

    e coli o157 h7 strain edl933 atcc 700927  (ATCC)


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    ATCC e coli o157 h7 strain edl933 atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    E Coli O157 H7 Strain Edl933 Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli"

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-12-231

    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Labeling

    Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Plasmid Preparation, Labeling

    SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Techniques Used: Mutagenesis, Western Blot, SDS Page

    SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Figure Legend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Techniques Used: Expressing, Mutagenesis, Labeling

    SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.
    Figure Legend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Techniques Used: Infection, Mutagenesis

    strain atcc 700927  (ATCC)


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    ATCC strain atcc 700927
    Strain Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ehec strain atcc 700927  (ATCC)


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    ATCC ehec strain atcc 700927
    Ehec Strain Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    strain atcc 700927  (ATCC)


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    ATCC strain atcc 700927
    Bacterial strains investigated in this study.
    Strain Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Impact of the rpoS genotype for acid resistance patterns of pathogenic and probiotic Escherichia coli"

    Article Title: Impact of the rpoS genotype for acid resistance patterns of pathogenic and probiotic Escherichia coli

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-7-21


    Figure Legend Snippet: Bacterial strains investigated in this study.

    Techniques Used:

    escherichia coli o157 h7 atcc 700927  (ATCC)


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    ATCC escherichia coli o157 h7 atcc 700927
    Nanonization of the major component of five different medicinal plants by different established methods of nanonization and their antibacterial potential on different bacteria.
    Escherichia Coli O157 H7 Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Nano-Antibacterials Using Medicinal Plant Components: An Overview"

    Article Title: Nano-Antibacterials Using Medicinal Plant Components: An Overview

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2021.768739

    Nanonization of the major component of five different medicinal plants by different established methods of nanonization and their antibacterial potential on different bacteria.
    Figure Legend Snippet: Nanonization of the major component of five different medicinal plants by different established methods of nanonization and their antibacterial potential on different bacteria.

    Techniques Used: Evaporation, Sonication

    Green synthesis of different metal and metal oxide NPs, using five different medicinal plant extracts and different established methods of nanonization, having antibacterial potential on different bacteria.
    Figure Legend Snippet: Green synthesis of different metal and metal oxide NPs, using five different medicinal plant extracts and different established methods of nanonization, having antibacterial potential on different bacteria.

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    e coli o157 h7 atcc 700927  (ATCC)


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    ATCC e coli o157 h7 atcc 700927
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    o157 atcc 700927  (ATCC)


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    ATCC o157 atcc 700927
    Anti-synthetic peptide sera response. Reactivity of sera anti-SP287/3, anti-SP459/1, anti-SP308/3, and anti-SP073/14 against synthetic peptides ( ); <t>E.</t> <t>coli</t> <t>O157,</t> S. urbana , S. arizonae , and S. typhi LPSs ( ); and the Ra core ( ) was evaluated by the ELISA test, as was previously mentioned in the Methods section. The pre-immune sera ( ) response is the average of the obtained results in the different assays.
    O157 Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria"

    Article Title: Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria

    Journal: Microorganisms

    doi: 10.3390/microorganisms8050780

    Anti-synthetic peptide sera response. Reactivity of sera anti-SP287/3, anti-SP459/1, anti-SP308/3, and anti-SP073/14 against synthetic peptides ( ); E. coli O157, S. urbana , S. arizonae , and S. typhi LPSs ( ); and the Ra core ( ) was evaluated by the ELISA test, as was previously mentioned in the Methods section. The pre-immune sera ( ) response is the average of the obtained results in the different assays.
    Figure Legend Snippet: Anti-synthetic peptide sera response. Reactivity of sera anti-SP287/3, anti-SP459/1, anti-SP308/3, and anti-SP073/14 against synthetic peptides ( ); E. coli O157, S. urbana , S. arizonae , and S. typhi LPSs ( ); and the Ra core ( ) was evaluated by the ELISA test, as was previously mentioned in the Methods section. The pre-immune sera ( ) response is the average of the obtained results in the different assays.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Recognition of the O157 LPS (FMU287) and R3 core by the anti-O157 LPS and the anti-SP 287/3 antibodies. Western blotting was carried out using a purified O157 LPS and R3 core. Lane 1, molecular weight marker; lane 2, O157 LPS revealed with pre-immune serum; lane 3, O157 LPS revealed with anti-O157 LPS serum; lane 4, O157 LPS revealed with anti-SP287/3 serum. The anti-O157 LPS and anti-SP287/3 antibodies recognized fractions of repeating carbohydrate subunits (stairway pattern) and the polysaccharide of the R3 core of the O157 LPS.
    Figure Legend Snippet: Recognition of the O157 LPS (FMU287) and R3 core by the anti-O157 LPS and the anti-SP 287/3 antibodies. Western blotting was carried out using a purified O157 LPS and R3 core. Lane 1, molecular weight marker; lane 2, O157 LPS revealed with pre-immune serum; lane 3, O157 LPS revealed with anti-O157 LPS serum; lane 4, O157 LPS revealed with anti-SP287/3 serum. The anti-O157 LPS and anti-SP287/3 antibodies recognized fractions of repeating carbohydrate subunits (stairway pattern) and the polysaccharide of the R3 core of the O157 LPS.

    Techniques Used: Western Blot, Purification, Molecular Weight, Marker

    Response of human serum samples (1:100) against lipopolysaccharides and synthetic peptides. The LPSs ( ) of E. coli O157 (FMU287), S. urbana (FMU459), S. arizonae (FMU308), and S. typhi (FMU073), as well as the synthetic peptides ( ) SP287/3, SP459/1, SP308/3, and SP073/14 were analyzed independently in an ELISA test assay against community serum samples, as described previously in the Methods section.
    Figure Legend Snippet: Response of human serum samples (1:100) against lipopolysaccharides and synthetic peptides. The LPSs ( ) of E. coli O157 (FMU287), S. urbana (FMU459), S. arizonae (FMU308), and S. typhi (FMU073), as well as the synthetic peptides ( ) SP287/3, SP459/1, SP308/3, and SP073/14 were analyzed independently in an ELISA test assay against community serum samples, as described previously in the Methods section.

    Techniques Used: Enzyme-linked Immunosorbent Assay

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    ATCC ehec o157 h7 edl933 strain atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
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    ATCC escherichia coli atcc 700927
    Sucrose-based autotroph-heterotroph co-cultures and their products.
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    ATCC edl933 atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
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    ATCC e coli o157 h7 strain edl933 atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    E Coli O157 H7 Strain Edl933 Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC strain atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Strain Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC ehec strain atcc 700927
    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC <t>EDL933</t> (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
    Ehec Strain Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC escherichia coli o157 h7 atcc 700927
    Nanonization of the major component of five different medicinal plants by different established methods of nanonization and their antibacterial potential on different bacteria.
    Escherichia Coli O157 H7 Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli o157 h7 atcc 700927
    Nanonization of the major component of five different medicinal plants by different established methods of nanonization and their antibacterial potential on different bacteria.
    E Coli O157 H7 Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC o157 atcc 700927
    Anti-synthetic peptide sera response. Reactivity of sera anti-SP287/3, anti-SP459/1, anti-SP308/3, and anti-SP073/14 against synthetic peptides ( ); <t>E.</t> <t>coli</t> <t>O157,</t> S. urbana , S. arizonae , and S. typhi LPSs ( ); and the Ra core ( ) was evaluated by the ELISA test, as was previously mentioned in the Methods section. The pre-immune sera ( ) response is the average of the obtained results in the different assays.
    O157 Atcc 700927, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

    Techniques: Expressing, Mutagenesis, Labeling

    Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

    Techniques: Expressing, Mutagenesis, Plasmid Preparation, Labeling

    SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

    Techniques: Mutagenesis, Western Blot, SDS Page

    SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

    Techniques: Expressing, Mutagenesis, Labeling

    SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Article Snippet: Gene deletions were constructed in EHEC O157:H7 EDL933 strain ATCC 700927 (Perna et al. 2001) by Lambda Red-mediated recombination using linear DNA fragments as described [ ].

    Techniques: Infection, Mutagenesis

    Journal: Frontiers in Microbiology

    Article Title: Cyanobacteria as cell factories for the photosynthetic production of sucrose

    doi: 10.3389/fmicb.2023.1126032

    Figure Lengend Snippet: Sucrose-based autotroph-heterotroph co-cultures and their products.

    Article Snippet: Similarly, S. elongatus PCC 7942 was originally engineered to export sucrose by expressing sucrose permease ( cscB ) from Escherichia coli ATCC 700927 , and multiple cyanobacterial species have since been similarly modified by different research teams ( ).

    Techniques:

    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: Overnight cultures of wild type and mutant derivatives of EDL933 ATCC 700927 were diluted 1:1000 in LB, supplemented with antibiotics if necessary, and grown aerobically at 37°C to an optical density at 600 nm (OD 600 ) of ~ 3.0 (stationary phase).

    Techniques: Expressing, Mutagenesis, Labeling

    Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: Overnight cultures of wild type and mutant derivatives of EDL933 ATCC 700927 were diluted 1:1000 in LB, supplemented with antibiotics if necessary, and grown aerobically at 37°C to an optical density at 600 nm (OD 600 ) of ~ 3.0 (stationary phase).

    Techniques: Expressing, Mutagenesis, Plasmid Preparation, Labeling

    SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Article Snippet: Overnight cultures of wild type and mutant derivatives of EDL933 ATCC 700927 were diluted 1:1000 in LB, supplemented with antibiotics if necessary, and grown aerobically at 37°C to an optical density at 600 nm (OD 600 ) of ~ 3.0 (stationary phase).

    Techniques: Mutagenesis, Western Blot, SDS Page

    SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: Overnight cultures of wild type and mutant derivatives of EDL933 ATCC 700927 were diluted 1:1000 in LB, supplemented with antibiotics if necessary, and grown aerobically at 37°C to an optical density at 600 nm (OD 600 ) of ~ 3.0 (stationary phase).

    Techniques: Expressing, Mutagenesis, Labeling

    SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Article Snippet: Overnight cultures of wild type and mutant derivatives of EDL933 ATCC 700927 were diluted 1:1000 in LB, supplemented with antibiotics if necessary, and grown aerobically at 37°C to an optical density at 600 nm (OD 600 ) of ~ 3.0 (stationary phase).

    Techniques: Infection, Mutagenesis

    SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA positively affects LEE expression in stationary phase cells. Primer extension analyses on total RNA extracted from wild type EHEC EDL933 (lane 1), the sspA mutant (lane 2) and the sspA mutant complemented with wild type sspA (lane 3) or mutant sspA84-86 (lane 4) as indicated, grown in LB at 37°C to stationary phase (OD 600 ~ 3.0). The Labeled DNA oligos specific to the transcripts of LEE1/ler ( A and I ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ) were used. The ompA transcripts, detected with a labeled ompA -specific DNA oligo, served as internal control for the primer extension reaction. Wild type and mutant SspA were expressed from pQE sspA and pQE sspA84-86 respectively in the absence of induction at similar levels. The transcripts LEE1-5, map, grlRA, stcE and the control transcript ompA are indicated. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

    Techniques: Expressing, Mutagenesis, Labeling

    Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: Increased ler expression overcomes repression of LEE in an sspA mutant. The expressions of virulence genes in wild type EHEC EDL933 (lane 1), the sspA mutant harboring the empty vector pACYC184 (pVector) (lane 2) and pACYC ler (p ler ) expressing ler from its native promoters (lane 3) were determined by primer extension analyses using labeled DNA oligos specific to LEE1/ler ( A ) , LEE2/espZ ( B ), LEE4/sepL ( C ), grlRA ( D ) and stcE ( E ) along with the ompA- specific oligo as a control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

    Techniques: Expressing, Mutagenesis, Plasmid Preparation, Labeling

    SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA negatively affects H-NS levels in EHEC. The levels of H-NS were determined in wild type (lane 1) and sspA mutant (lane 2) derivatives of EHEC EDL933 grown to stationary phase cells by western blot. Equal amounts of total protein were resolved on a 10% Bis-Tris SDS-PAGE gel and transferred to a nitrocellulose membrane. Levels of H-NS and Fis were detected using polyclonal antibodies against the respective proteins. Fis served as an internal control for total protein levels. The relative amounts of H-NS normalized to that of Fis are indicated by the numbers in parenthesis.

    Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

    Techniques: Mutagenesis, Western Blot, SDS Page

    SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2) , hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler ( A ) , LEE2/espZ ( B ) , LEE3/mpc ( C ) , LEE4/sepL ( D ), LEE5/tir ( E ), map ( F ), grlRA ( G ) and stcE ( H ). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure . The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.

    Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

    Techniques: Expressing, Mutagenesis, Labeling

    SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Journal: BMC Microbiology

    Article Title: SspA up-regulates gene expression of the LEE pathogenicity island by decreasing H-NS levels in enterohemorrhagic Escherichia coli

    doi: 10.1186/1471-2180-12-231

    Figure Lengend Snippet: SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 ( A ) and its mutant derivatives of sspA ( B ), sspA pQE sspA ( C ), sspA pQE sspA84-86 ( D ), hns ( E ) and hns sspA ( F ). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.

    Article Snippet: To evaluate the effect of sspA on virulence gene expression in EHEC during the stationary phase we constructed an in-frame deletion of sspA in the E. coli O157:H7 strain EDL933 ATCC 700927 [ ] and measured transcription of LEE- ( LEE1-5 , grlRA and map ) and non-LEE-encoded ( stcE encoded by pO157) genes (Figure ).

    Techniques: Infection, Mutagenesis

    Nanonization of the major component of five different medicinal plants by different established methods of nanonization and their antibacterial potential on different bacteria.

    Journal: Frontiers in Microbiology

    Article Title: Nano-Antibacterials Using Medicinal Plant Components: An Overview

    doi: 10.3389/fmicb.2021.768739

    Figure Lengend Snippet: Nanonization of the major component of five different medicinal plants by different established methods of nanonization and their antibacterial potential on different bacteria.

    Article Snippet: Nanonization of oregano oil has been made by the “oil in water” nanoemulsion method by simply mixing oregano oil and the surfactant Tween 80 in water in 2:1 ratio, followed by sonication for 10 min, and this nanoformulation is found to control foodborne bacterial pathogens Listeria monocytogenes ATCC 19115, Salmonella typhimurium ATCC 19585, and Escherichia coli O157:H7 ATCC 700927 on lettuce leaves ( ).

    Techniques: Evaporation, Sonication

    Green synthesis of different metal and metal oxide NPs, using five different medicinal plant extracts and different established methods of nanonization, having antibacterial potential on different bacteria.

    Journal: Frontiers in Microbiology

    Article Title: Nano-Antibacterials Using Medicinal Plant Components: An Overview

    doi: 10.3389/fmicb.2021.768739

    Figure Lengend Snippet: Green synthesis of different metal and metal oxide NPs, using five different medicinal plant extracts and different established methods of nanonization, having antibacterial potential on different bacteria.

    Article Snippet: Nanonization of oregano oil has been made by the “oil in water” nanoemulsion method by simply mixing oregano oil and the surfactant Tween 80 in water in 2:1 ratio, followed by sonication for 10 min, and this nanoformulation is found to control foodborne bacterial pathogens Listeria monocytogenes ATCC 19115, Salmonella typhimurium ATCC 19585, and Escherichia coli O157:H7 ATCC 700927 on lettuce leaves ( ).

    Techniques:

    Anti-synthetic peptide sera response. Reactivity of sera anti-SP287/3, anti-SP459/1, anti-SP308/3, and anti-SP073/14 against synthetic peptides ( ); E. coli O157, S. urbana , S. arizonae , and S. typhi LPSs ( ); and the Ra core ( ) was evaluated by the ELISA test, as was previously mentioned in the Methods section. The pre-immune sera ( ) response is the average of the obtained results in the different assays.

    Journal: Microorganisms

    Article Title: Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria

    doi: 10.3390/microorganisms8050780

    Figure Lengend Snippet: Anti-synthetic peptide sera response. Reactivity of sera anti-SP287/3, anti-SP459/1, anti-SP308/3, and anti-SP073/14 against synthetic peptides ( ); E. coli O157, S. urbana , S. arizonae , and S. typhi LPSs ( ); and the Ra core ( ) was evaluated by the ELISA test, as was previously mentioned in the Methods section. The pre-immune sera ( ) response is the average of the obtained results in the different assays.

    Article Snippet: The strains of E. coli that were used to obtain the LPSs and cores were as follows: O157 ATCC 700927, coded in the strain collection of the laboratory of the Faculty of Medicine UNAM (FMU) as 287 (FMU287); the cores were derived from mutant strains of E. coli , R1 (O8:K27, F470), R2 (O100:K?B:H2, F632), R3 (O111:K58:H-, F653), R4 (O14:K7, F2513), and K12 (O16, W3110) [ ]; the strains of Salmonella , including the following: S. urbana (FMU459), S. arizonae (FMU308), and S. typhi ATCC 6539 (FMU073), which also came from the Faculty of Medicine, UNAM; the core Ra came from S. minnesota (List Biological Laboratories, Campbell, C A, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    Recognition of the O157 LPS (FMU287) and R3 core by the anti-O157 LPS and the anti-SP 287/3 antibodies. Western blotting was carried out using a purified O157 LPS and R3 core. Lane 1, molecular weight marker; lane 2, O157 LPS revealed with pre-immune serum; lane 3, O157 LPS revealed with anti-O157 LPS serum; lane 4, O157 LPS revealed with anti-SP287/3 serum. The anti-O157 LPS and anti-SP287/3 antibodies recognized fractions of repeating carbohydrate subunits (stairway pattern) and the polysaccharide of the R3 core of the O157 LPS.

    Journal: Microorganisms

    Article Title: Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria

    doi: 10.3390/microorganisms8050780

    Figure Lengend Snippet: Recognition of the O157 LPS (FMU287) and R3 core by the anti-O157 LPS and the anti-SP 287/3 antibodies. Western blotting was carried out using a purified O157 LPS and R3 core. Lane 1, molecular weight marker; lane 2, O157 LPS revealed with pre-immune serum; lane 3, O157 LPS revealed with anti-O157 LPS serum; lane 4, O157 LPS revealed with anti-SP287/3 serum. The anti-O157 LPS and anti-SP287/3 antibodies recognized fractions of repeating carbohydrate subunits (stairway pattern) and the polysaccharide of the R3 core of the O157 LPS.

    Article Snippet: The strains of E. coli that were used to obtain the LPSs and cores were as follows: O157 ATCC 700927, coded in the strain collection of the laboratory of the Faculty of Medicine UNAM (FMU) as 287 (FMU287); the cores were derived from mutant strains of E. coli , R1 (O8:K27, F470), R2 (O100:K?B:H2, F632), R3 (O111:K58:H-, F653), R4 (O14:K7, F2513), and K12 (O16, W3110) [ ]; the strains of Salmonella , including the following: S. urbana (FMU459), S. arizonae (FMU308), and S. typhi ATCC 6539 (FMU073), which also came from the Faculty of Medicine, UNAM; the core Ra came from S. minnesota (List Biological Laboratories, Campbell, C A, USA).

    Techniques: Western Blot, Purification, Molecular Weight, Marker

    Response of human serum samples (1:100) against lipopolysaccharides and synthetic peptides. The LPSs ( ) of E. coli O157 (FMU287), S. urbana (FMU459), S. arizonae (FMU308), and S. typhi (FMU073), as well as the synthetic peptides ( ) SP287/3, SP459/1, SP308/3, and SP073/14 were analyzed independently in an ELISA test assay against community serum samples, as described previously in the Methods section.

    Journal: Microorganisms

    Article Title: Phage Display Detection of Mimotopes that Are Shared Epitopes of Clinically and Epidemiologically Relevant Enterobacteria

    doi: 10.3390/microorganisms8050780

    Figure Lengend Snippet: Response of human serum samples (1:100) against lipopolysaccharides and synthetic peptides. The LPSs ( ) of E. coli O157 (FMU287), S. urbana (FMU459), S. arizonae (FMU308), and S. typhi (FMU073), as well as the synthetic peptides ( ) SP287/3, SP459/1, SP308/3, and SP073/14 were analyzed independently in an ELISA test assay against community serum samples, as described previously in the Methods section.

    Article Snippet: The strains of E. coli that were used to obtain the LPSs and cores were as follows: O157 ATCC 700927, coded in the strain collection of the laboratory of the Faculty of Medicine UNAM (FMU) as 287 (FMU287); the cores were derived from mutant strains of E. coli , R1 (O8:K27, F470), R2 (O100:K?B:H2, F632), R3 (O111:K58:H-, F653), R4 (O14:K7, F2513), and K12 (O16, W3110) [ ]; the strains of Salmonella , including the following: S. urbana (FMU459), S. arizonae (FMU308), and S. typhi ATCC 6539 (FMU073), which also came from the Faculty of Medicine, UNAM; the core Ra came from S. minnesota (List Biological Laboratories, Campbell, C A, USA).

    Techniques: Enzyme-linked Immunosorbent Assay