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Promega erase a base system
Detailed map of deletions generated in pDR12. Deletions were generated by the <t>Erase-a-Base</t> system (see Materials and Methods). The extent of each deletion is shown by a thin line. Mucoidy and induction were scored in strain B6905(λ) (Δ recA ) transformed with the various plasmids. Abbreviations for restrictions enzymes are as follows: E, Eco RI; H, Hin dIII; N, Nsi I; S, Sph I; X, Xba I.
Erase A Base System, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erase a base system/product/Promega
Average 91 stars, based on 22 article reviews
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1) Product Images from "RecA-Independent Pathways of Lambdoid Prophage Induction in Escherichia coli"

Article Title: RecA-Independent Pathways of Lambdoid Prophage Induction in Escherichia coli

Journal: Journal of Bacteriology

doi:

Detailed map of deletions generated in pDR12. Deletions were generated by the Erase-a-Base system (see Materials and Methods). The extent of each deletion is shown by a thin line. Mucoidy and induction were scored in strain B6905(λ) (Δ recA ) transformed with the various plasmids. Abbreviations for restrictions enzymes are as follows: E, Eco RI; H, Hin dIII; N, Nsi I; S, Sph I; X, Xba I.
Figure Legend Snippet: Detailed map of deletions generated in pDR12. Deletions were generated by the Erase-a-Base system (see Materials and Methods). The extent of each deletion is shown by a thin line. Mucoidy and induction were scored in strain B6905(λ) (Δ recA ) transformed with the various plasmids. Abbreviations for restrictions enzymes are as follows: E, Eco RI; H, Hin dIII; N, Nsi I; S, Sph I; X, Xba I.

Techniques Used: Generated, Transformation Assay

Related Articles

Sequencing:

Article Title: Purification and characterization of a human RNA adenosine deaminase for glutamate receptor B pre-mRNA editing
Article Snippet: .. To determine the sequence, the insert was deleted from the 5′ and 3′ ends using the Erase-a-Base system (Promega) and sequenced by dideoxy methods. .. The human and rat RED1 cDNAs were subcloned into pFastBac-HTb and proteins were expressed in Sf9 cells (GIBCO/BRL).

Article Title: Molecular Characterization of the Hemin Uptake Locus (hmu) from Yersinia pestis and Analysis of hmu Mutants for Hemin and Hemoprotein Utilization
Article Snippet: .. Extension of the nucleotide sequence on either DNA strand was obtained by using oligonucleotide primers (Integrated DNA Technologies, Inc.) or M13 forward/reverse primers on various subclones of pHMU4 or subclones generated from unidirectional nested deletions with the Erase-a-Base system, as described by Promega Corp. (Madison, Wis.). .. To sequence directly the hmuP′ gene from the chromosome, we amplified a 274-bp region from Y. pestis KIM6+ or KIM6 chromosome by using primers 5′-TGAGCCAGGATTAGCCCGTAAG-3′ and 5′-GCATTCGCCCTGATGGACGATA-3′, treated the PCR-generated products with Klenow fragment, and cloned the products into pBluescriptII KS+.

Clone Assay:

Article Title: Fusion of the transcription factorTFE3 gene to a novel gene,PRCC, in t(X;1)(p11;q21)-positive papillary renal cell carcinomas
Article Snippet: .. A set of overlapping deletion clones was constructed using the Erase-a-Base system (Promega). .. DNA sequences were analyzed on an automated DNA sequencer (Applied Biosystems model no. 373A) using the Taq Dye Deoxy Terminator Cycle Sequencing kit (Applied Biosystems/Perkin–Elmer).

Generated:

Article Title: Molecular Characterization of the Hemin Uptake Locus (hmu) from Yersinia pestis and Analysis of hmu Mutants for Hemin and Hemoprotein Utilization
Article Snippet: .. Extension of the nucleotide sequence on either DNA strand was obtained by using oligonucleotide primers (Integrated DNA Technologies, Inc.) or M13 forward/reverse primers on various subclones of pHMU4 or subclones generated from unidirectional nested deletions with the Erase-a-Base system, as described by Promega Corp. (Madison, Wis.). .. To sequence directly the hmuP′ gene from the chromosome, we amplified a 274-bp region from Y. pestis KIM6+ or KIM6 chromosome by using primers 5′-TGAGCCAGGATTAGCCCGTAAG-3′ and 5′-GCATTCGCCCTGATGGACGATA-3′, treated the PCR-generated products with Klenow fragment, and cloned the products into pBluescriptII KS+.

Article Title: The Oct DNA motif participates in the alcohol inhibition of the inducible nitric-oxide synthase gene promoter in rat C6 glioma cells
Article Snippet: .. The smaller piNOS-luc-78 promoter was generated from the piNOS-luc-94 promoter construct using the Erase-A-Base system (Promega) following the general procedures recommended by the manufacturer. .. Briefly, the piNOS-luc-94 promoter construct was linearized and an Exo III resistant 3′ overhang was generated by digestion with Kpn I.

Construct:

Article Title: Fusion of the transcription factorTFE3 gene to a novel gene,PRCC, in t(X;1)(p11;q21)-positive papillary renal cell carcinomas
Article Snippet: .. A set of overlapping deletion clones was constructed using the Erase-a-Base system (Promega). .. DNA sequences were analyzed on an automated DNA sequencer (Applied Biosystems model no. 373A) using the Taq Dye Deoxy Terminator Cycle Sequencing kit (Applied Biosystems/Perkin–Elmer).

Article Title: The Oct DNA motif participates in the alcohol inhibition of the inducible nitric-oxide synthase gene promoter in rat C6 glioma cells
Article Snippet: .. The smaller piNOS-luc-78 promoter was generated from the piNOS-luc-94 promoter construct using the Erase-A-Base system (Promega) following the general procedures recommended by the manufacturer. .. Briefly, the piNOS-luc-94 promoter construct was linearized and an Exo III resistant 3′ overhang was generated by digestion with Kpn I.

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  • 85
    Promega promega erase a base kit
    Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the <t>Promega</t> <t>Erase-A-Base</t> Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .
    Promega Erase A Base Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/promega erase a base kit/product/Promega
    Average 85 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    promega erase a base kit - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Promega erase a base method
    obg ( yhbZ ) subclones sequenced from both ends with the <t>Erase-A-Base</t> system. (A) All clones that conferred rescue of the rrmJ deletion phenotype contained the complete obg open reading frame. (B) All clones that did not rescue the rrmJ deletion growth defect did not contain the entire obg gene. obg is shown in complement, and deletions were from the 3′ end of the gene.
    Erase A Base Method, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erase a base method/product/Promega
    Average 85 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    erase a base method - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the Promega Erase-A-Base Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .

    Journal: F1000Research

    Article Title: Identification of two telomere-proximal fission yeast DNA replication origins constrained by nearby cis-acting sequences to replicate in late S phase

    doi: 10.12688/f1000research.1-58.v1

    Figure Lengend Snippet: Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the Promega Erase-A-Base Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .

    Article Snippet: The Promega Erase-a-Base Kit, which employs exonuclease III, was used to make the deletions indicated in .

    Techniques: Construct

    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

    Journal: The Journal of Biological Chemistry

    Article Title: Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *

    doi: 10.1074/jbc.M114.548446

    Figure Lengend Snippet: Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

    Article Snippet: The pGL3−1416/+219 vector was used as a template to generate a series of PRKCE promoter truncated luciferase reporter vectors (−1319/+219, −1224/+219, −1121/+219, −1032/+219, −1028/+219, −921/+219, −887/+219, −873/+219, −819/+219, −796/+219, and −777/+219) with the Erase-a-Base kit (Promega, Madison, WI). pGL3−644/+219 was generated by digestion of pGL3−808/+219 vector with PfIMI and NheI and subsequent religation.

    Techniques: Transfection, Generated, Plasmid Preparation, Luciferase, Activity Assay

    obg ( yhbZ ) subclones sequenced from both ends with the Erase-A-Base system. (A) All clones that conferred rescue of the rrmJ deletion phenotype contained the complete obg open reading frame. (B) All clones that did not rescue the rrmJ deletion growth defect did not contain the entire obg gene. obg is shown in complement, and deletions were from the 3′ end of the gene.

    Journal: Journal of Bacteriology

    Article Title: Overexpression of Two Different GTPases Rescues a Null Mutation in a Heat-Induced rRNA Methyltransferase

    doi: 10.1128/JB.184.10.2692-2698.2002

    Figure Lengend Snippet: obg ( yhbZ ) subclones sequenced from both ends with the Erase-A-Base system. (A) All clones that conferred rescue of the rrmJ deletion phenotype contained the complete obg open reading frame. (B) All clones that did not rescue the rrmJ deletion growth defect did not contain the entire obg gene. obg is shown in complement, and deletions were from the 3′ end of the gene.

    Article Snippet: To determine if the open reading frames were directly responsible for the observed rescue, we created subclones of one of the obg ( yhbZ )-containing clones by using the Erase-a-base method (Promega).

    Techniques: Clone Assay

    Detailed map of deletions generated in pDR12. Deletions were generated by the Erase-a-Base system (see Materials and Methods). The extent of each deletion is shown by a thin line. Mucoidy and induction were scored in strain B6905(λ) (Δ recA ) transformed with the various plasmids. Abbreviations for restrictions enzymes are as follows: E, Eco RI; H, Hin dIII; N, Nsi I; S, Sph I; X, Xba I.

    Journal: Journal of Bacteriology

    Article Title: RecA-Independent Pathways of Lambdoid Prophage Induction in Escherichia coli

    doi:

    Figure Lengend Snippet: Detailed map of deletions generated in pDR12. Deletions were generated by the Erase-a-Base system (see Materials and Methods). The extent of each deletion is shown by a thin line. Mucoidy and induction were scored in strain B6905(λ) (Δ recA ) transformed with the various plasmids. Abbreviations for restrictions enzymes are as follows: E, Eco RI; H, Hin dIII; N, Nsi I; S, Sph I; X, Xba I.

    Article Snippet: Then pDR12 was digested again with Xba I or Hin dIII, and DNA deletion with exonuclease III was carried out as described in the Erase-a-Base system from Promega Biotec.

    Techniques: Generated, Transformation Assay