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Promega erase a base system kit
Erase A Base System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erase a base system kit/product/Promega
Average 80 stars, based on 2 article reviews
Price from $9.99 to $1999.99
erase a base system kit - by Bioz Stars, 2020-09
80/100 stars

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unidirectional nested deletions
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Article Title: MsPG3, a Medicago sativa polygalacturonase gene expressed during the alfalfa–Rhizobium meliloti interaction
Article Snippet: For fragments longer than 0.5 kb, deletion derivatives were obtained by using the Erase-a-Base System Kit (Promega).

Article Title: RpmA Is Required for Nonopsonic Phagocytosis of Pseudomonas aeruginosa
Article Snippet: A series of unidirectional nested deletions of pDS27.9 were created using the Erase-a-Base System kit (Promega).

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    Promega promega erase a base kit
    Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the <t>Promega</t> <t>Erase-A-Base</t> Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .
    Promega Erase A Base Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/promega erase a base kit/product/Promega
    Average 85 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    promega erase a base kit - by Bioz Stars, 2020-09
    85/100 stars
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    Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the Promega Erase-A-Base Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .

    Journal: F1000Research

    Article Title: Identification of two telomere-proximal fission yeast DNA replication origins constrained by nearby cis-acting sequences to replicate in late S phase

    doi: 10.12688/f1000research.1-58.v1

    Figure Lengend Snippet: Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the Promega Erase-A-Base Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .

    Article Snippet: The Promega Erase-a-Base Kit, which employs exonuclease III, was used to make the deletions indicated in .

    Techniques: Construct

    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

    Journal: The Journal of Biological Chemistry

    Article Title: Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *

    doi: 10.1074/jbc.M114.548446

    Figure Lengend Snippet: Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

    Article Snippet: The pGL3−1416/+219 vector was used as a template to generate a series of PRKCE promoter truncated luciferase reporter vectors (−1319/+219, −1224/+219, −1121/+219, −1032/+219, −1028/+219, −921/+219, −887/+219, −873/+219, −819/+219, −796/+219, and −777/+219) with the Erase-a-Base kit (Promega, Madison, WI). pGL3−644/+219 was generated by digestion of pGL3−808/+219 vector with PfIMI and NheI and subsequent religation.

    Techniques: Transfection, Generated, Plasmid Preparation, Luciferase, Activity Assay