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Promega erase a base kit
Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
Erase A Base Kit, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erase a base kit/product/Promega
Average 89 stars, based on 50 article reviews
Price from $9.99 to $1999.99
erase a base kit - by Bioz Stars, 2020-09
89/100 stars

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1) Product Images from "Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *"

Article Title: Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M114.548446

Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
Figure Legend Snippet: Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

Techniques Used: Transfection, Generated, Plasmid Preparation, Luciferase, Activity Assay

Related Articles

Sequencing:

Article Title: Characterization of Mouse Fibronectin Alternative mRNAs Reveals an Unusual Isoform Present Transiently During Liver Development
Article Snippet: .. Sequence was obtained from nested deletions generated using the Erase-A-Base kit (Promega Biotech) or from various subclones, and was determined from both strands. ..

Article Title: Characterization and Expression of Four Proline-Rich Cell Wall Protein Genes in Arabidopsis Encoding Two Distinct Subsets of Multiple Domain Proteins 1
Article Snippet: .. AtPRP subclones were digested with Exonuclease III using an Erase-a-Base kit (Promega, Madison, WI) and the series of nested deletions were sequenced using either Sequenase (United States Biochemical, Cleveland) or Taq DNA polymerase and a dsDNA cycle sequencing kit (BRL, Gaithersburg, MD). .. AtPRP cDNA clones were isolated from a λPRL2 library ( ) screened with AtPRP probes pH/R2 (Fig. , bp 717–1,455) and pH/ Sau 3A (Fig. , bp 190–865).

Polymerase Chain Reaction:

Article Title: Construction of In-Frame aroA Deletion Mutants of Mannheimia haemolytica, Pasteurella multocida, and Haemophilus somnus by Using a New Temperature-Sensitive Plasmid
Article Snippet: .. The resultant PCR fragment was ligated into pCR2.1 and then excised by EcoRI digestion and transferred to the EcoRI site of pGA301 ori C to generate replacement plasmid pTs Hsaro C. A deletion was introduced within the gene's coding region by digestion with BspEI followed by exonuclease III and S1 nuclease treatment using an Erase-a-base kit (Promega Corp., Madison, WI). .. The plasmid was then digested at a unique MunI site within aroA and treated with the Klenow fragment and nucleoside triphosphates to make the fragment ends blunt.

Generated:

Article Title: Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *
Article Snippet: .. The pGL3−1416/+219 vector was used as a template to generate a series of PRKCE promoter truncated luciferase reporter vectors (−1319/+219, −1224/+219, −1121/+219, −1032/+219, −1028/+219, −921/+219, −887/+219, −873/+219, −819/+219, −796/+219, and −777/+219) with the Erase-a-Base kit (Promega, Madison, WI). pGL3−644/+219 was generated by digestion of pGL3−808/+219 vector with PfIMI and NheI and subsequent religation. ..

Article Title: Characterization of Mouse Fibronectin Alternative mRNAs Reveals an Unusual Isoform Present Transiently During Liver Development
Article Snippet: .. Sequence was obtained from nested deletions generated using the Erase-A-Base kit (Promega Biotech) or from various subclones, and was determined from both strands. ..

Luciferase:

Article Title: Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *
Article Snippet: .. The pGL3−1416/+219 vector was used as a template to generate a series of PRKCE promoter truncated luciferase reporter vectors (−1319/+219, −1224/+219, −1121/+219, −1032/+219, −1028/+219, −921/+219, −887/+219, −873/+219, −819/+219, −796/+219, and −777/+219) with the Erase-a-Base kit (Promega, Madison, WI). pGL3−644/+219 was generated by digestion of pGL3−808/+219 vector with PfIMI and NheI and subsequent religation. ..

Plasmid Preparation:

Article Title: Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *
Article Snippet: .. The pGL3−1416/+219 vector was used as a template to generate a series of PRKCE promoter truncated luciferase reporter vectors (−1319/+219, −1224/+219, −1121/+219, −1032/+219, −1028/+219, −921/+219, −887/+219, −873/+219, −819/+219, −796/+219, and −777/+219) with the Erase-a-Base kit (Promega, Madison, WI). pGL3−644/+219 was generated by digestion of pGL3−808/+219 vector with PfIMI and NheI and subsequent religation. ..

Article Title: Construction of In-Frame aroA Deletion Mutants of Mannheimia haemolytica, Pasteurella multocida, and Haemophilus somnus by Using a New Temperature-Sensitive Plasmid
Article Snippet: .. The resultant PCR fragment was ligated into pCR2.1 and then excised by EcoRI digestion and transferred to the EcoRI site of pGA301 ori C to generate replacement plasmid pTs Hsaro C. A deletion was introduced within the gene's coding region by digestion with BspEI followed by exonuclease III and S1 nuclease treatment using an Erase-a-base kit (Promega Corp., Madison, WI). .. The plasmid was then digested at a unique MunI site within aroA and treated with the Klenow fragment and nucleoside triphosphates to make the fragment ends blunt.

Article Title: The conserved upstream region of lscB/C determines expression of different levansucrase genes in plant pathogen Pseudomonas syringae
Article Snippet: .. Nested deletion analysis of the upstream region of lscB in plasmid pRB7.2 [ ] was conducted using the Erase-a-Base kit (Promega, Madison, USA). .. For analysis of the lsc upstream regions, PCR was used to generate products covering the respective regions (Table ).

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    Promega promega erase a base kit
    Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the <t>Promega</t> <t>Erase-A-Base</t> Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .
    Promega Erase A Base Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/promega erase a base kit/product/Promega
    Average 85 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    promega erase a base kit - by Bioz Stars, 2020-09
    85/100 stars
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    Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the Promega Erase-A-Base Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .

    Journal: F1000Research

    Article Title: Identification of two telomere-proximal fission yeast DNA replication origins constrained by nearby cis-acting sequences to replicate in late S phase

    doi: 10.12688/f1000research.1-58.v1

    Figure Lengend Snippet: Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the Promega Erase-A-Base Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .

    Article Snippet: The Promega Erase-a-Base Kit, which employs exonuclease III, was used to make the deletions indicated in .

    Techniques: Construct

    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

    Journal: The Journal of Biological Chemistry

    Article Title: Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *

    doi: 10.1074/jbc.M114.548446

    Figure Lengend Snippet: Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

    Article Snippet: The pGL3−1416/+219 vector was used as a template to generate a series of PRKCE promoter truncated luciferase reporter vectors (−1319/+219, −1224/+219, −1121/+219, −1032/+219, −1028/+219, −921/+219, −887/+219, −873/+219, −819/+219, −796/+219, and −777/+219) with the Erase-a-Base kit (Promega, Madison, WI). pGL3−644/+219 was generated by digestion of pGL3−808/+219 vector with PfIMI and NheI and subsequent religation.

    Techniques: Transfection, Generated, Plasmid Preparation, Luciferase, Activity Assay