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GE Healthcare erase a base kit
Erase A Base Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erase a base kit/product/GE Healthcare
Average 94 stars, based on 2 article reviews
Price from $9.99 to $1999.99
erase a base kit - by Bioz Stars, 2020-09
94/100 stars

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Article Title: Cloning and Expression of the Lactococcus lactis purDEK Genes, Required for Growth in Milk
Article Snippet: To map the location of the promoter, a series of nested deletions of the 846-bp fragment was made with exonuclease III, which was included in the Erase-a-Base kit (Amersham).

Plasmid Preparation:

Article Title: Cloning and Expression of the Lactococcus lactis purDEK Genes, Required for Growth in Milk
Article Snippet: .. The plasmid was then digested with exonuclease III from the Not I site at 21°C for various times ranging from 1 to 10 min. All other steps recommended for the Erase-A-Base kit were performed as suggested by the supplier (Amersham). .. After ligation of the various deleted variants of the plasmid pLN71, the plasmids were transformed into L. lactis MG1363 and plated on DN medium agar plates containing X-Gal (5-bromo-4-chloro-3-indolyl-β- d -glucuronic acid) for screening of β-galactosidase activity.

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    GE Healthcare illustra genomiphi v2 dna amplification kit
    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare <t>illustra</t> <t>GenomiPhi</t> <t>V2</t> DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded
    Illustra Genomiphi V2 Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 182 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illustra genomiphi v2 dna amplification kit/product/GE Healthcare
    Average 99 stars, based on 182 article reviews
    Price from $9.99 to $1999.99
    illustra genomiphi v2 dna amplification kit - by Bioz Stars, 2020-09
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    99
    GE Healthcare cy3
    Downregulating Nlg1 expression increases AMPAR membrane diffusion. A , Live labeling of endogenous surface Nlg1 using soluble Nrx1β-Fc in 1- week-old rat or mouse neurons, upon Nlg1 overexpression or downregulation. The clustered appearance of overexpressed Nlg1 was comparable to that of endogenous Nlg1 (insets). Nlg1 cluster density was 0.24 ± 0.03/μm 2 for transfected cells versus 0.08 ± 0.01/μm 2 for nontransfected cells, and cluster size was 0.51 ± 0.02 μm 2 versus 0.24 ± 0.02 μm 2 , respectively ( n = 7 neurons). B , Quantification of the <t>Cy3-conjugated</t> anti-Fc labeling in arbitrary fluorescence units. The number of cells examined in each condition is indicated in the columns (2 independent experiments). C , AMPAR diffusion was measured using anti-GluA2-conjugated Qdots in primary 9–10 DIV neurons obtained from either WT or Nlg1 KO mouse pups. In some experiments, KO cultures were transfected with Nlg1WT, and WT cultures with Nlg1WT or shRNA against Nlg1. D , Diffusion coefficients were computed for 150–2400 trajectories from 6 independent experiments. Statistical P- values: * p
    Cy3, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3/product/GE Healthcare
    Average 99 stars, based on 103 article reviews
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    GE Healthcare cy5 labeled
    2-D gel image of the master gel with labeled picked proteins spots. Proteome pattern of canine liver tissue by means of 2D –DIGE. For protein analysis, proteins were labeled with Cy2, Cy3, and <t>Cy5,</t> separated in the first dimension using an immobilized pH gradient, and subsequently, in the second dimension, by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Protein spots were detected using a fluorescence scanner (MWM–molecular weight marker; kDA–kilodalton, NL–nonlinear).
    Cy5 Labeled, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy5 labeled/product/GE Healthcare
    Average 99 stars, based on 16 article reviews
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    95
    GE Healthcare cyclic adenosine monophosphate camp enzyme immunoassay enzyme immunoassay eia kit
    Cyclic adenosine <t>monophosphate</t> (cAMP) levels in isolated rats cardiomyocytes, measured by enzyme immunoassay <t>(EIA).</t> Dose-response relationship with increasing concentrations of iloprost (1–3) in the cell culture plates. OD: optical density; 1:
    Cyclic Adenosine Monophosphate Camp Enzyme Immunoassay Enzyme Immunoassay Eia Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclic adenosine monophosphate camp enzyme immunoassay enzyme immunoassay eia kit/product/GE Healthcare
    Average 95 stars, based on 1 article reviews
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    A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

    Journal: GigaScience

    Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing

    doi: 10.1186/s13742-015-0068-3

    Figure Lengend Snippet: A narrowing-down strategy used to compare WGA methods cost-effectively. We describe the narrowing-down strategy using 3 panels ( a , b , c ). We perform LWGS comparison including genome coverage and uniform using YH single cells which are amplified by seven WGA kits based on DOP, MDA and MALBAC methods in panel A. We additionally compare the CNVs detection using simulated data of YH single cells in panel A. In panel B, we perform the deep WGS comparison of biases and SNVs detection using deep-sequenced YH or SW480 single cells amplified by DOP, MDA or MALBAC respectively. Corresponding bulk data is used as unamplified control. In panel C, we further compare the CNVs detection between MDA-2 and MALBAC amplified data using real data of BGC823 single cells. *Ion Proton sequencing data; #Illumina and Ion Proton sequencing data. LWGS, low-coverage whole-genome sequencing; WGS: whole genome sequencing. DOP-1,GenomePlex® Single Cell WGA Kit; DOP-2, Silicon Biosystem Ampli ™ WGA Kit; DOP-3, NEB Single Cell WGA Kit; MDA-1, Qiagen REPLI-g Mini Kit; MDA-2, Qiagen REPLI-g Single Cell Kit; MDA-3, GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit; MALBAC,Yikon Genomics Single Cell Whole Genome Amplification Kit. Data marked in purple is downloaded

    Article Snippet: The kits used were Qiagen REPLI-g Mini Kit (MDA-1), Qiagen REPLI-g Single Cell Kit (MDA-2), GE Healthcare illustra GenomiPhi V2 DNA Amplification Kit (MDA-3), GenomePlex® Single Cell WGA Kit (DOP-1), Silicon Biosystem Ampli1™ WGA Kit (DOP-2), NEB Single Cell WGA Kit (DOP-3), and Yikon Genomics Single Cell WGA Kit (MALBAC).

    Techniques: Whole Genome Amplification, Amplification, Multiple Displacement Amplification, Multiple Annealing and Looping–Based Amplification Cycles, Sequencing

    Downregulating Nlg1 expression increases AMPAR membrane diffusion. A , Live labeling of endogenous surface Nlg1 using soluble Nrx1β-Fc in 1- week-old rat or mouse neurons, upon Nlg1 overexpression or downregulation. The clustered appearance of overexpressed Nlg1 was comparable to that of endogenous Nlg1 (insets). Nlg1 cluster density was 0.24 ± 0.03/μm 2 for transfected cells versus 0.08 ± 0.01/μm 2 for nontransfected cells, and cluster size was 0.51 ± 0.02 μm 2 versus 0.24 ± 0.02 μm 2 , respectively ( n = 7 neurons). B , Quantification of the Cy3-conjugated anti-Fc labeling in arbitrary fluorescence units. The number of cells examined in each condition is indicated in the columns (2 independent experiments). C , AMPAR diffusion was measured using anti-GluA2-conjugated Qdots in primary 9–10 DIV neurons obtained from either WT or Nlg1 KO mouse pups. In some experiments, KO cultures were transfected with Nlg1WT, and WT cultures with Nlg1WT or shRNA against Nlg1. D , Diffusion coefficients were computed for 150–2400 trajectories from 6 independent experiments. Statistical P- values: * p

    Journal: The Journal of Neuroscience

    Article Title: Neurexin-Neuroligin Adhesions Capture Surface-Diffusing AMPA Receptors through PSD-95 Scaffolds

    doi: 10.1523/JNEUROSCI.6439-10.2011

    Figure Lengend Snippet: Downregulating Nlg1 expression increases AMPAR membrane diffusion. A , Live labeling of endogenous surface Nlg1 using soluble Nrx1β-Fc in 1- week-old rat or mouse neurons, upon Nlg1 overexpression or downregulation. The clustered appearance of overexpressed Nlg1 was comparable to that of endogenous Nlg1 (insets). Nlg1 cluster density was 0.24 ± 0.03/μm 2 for transfected cells versus 0.08 ± 0.01/μm 2 for nontransfected cells, and cluster size was 0.51 ± 0.02 μm 2 versus 0.24 ± 0.02 μm 2 , respectively ( n = 7 neurons). B , Quantification of the Cy3-conjugated anti-Fc labeling in arbitrary fluorescence units. The number of cells examined in each condition is indicated in the columns (2 independent experiments). C , AMPAR diffusion was measured using anti-GluA2-conjugated Qdots in primary 9–10 DIV neurons obtained from either WT or Nlg1 KO mouse pups. In some experiments, KO cultures were transfected with Nlg1WT, and WT cultures with Nlg1WT or shRNA against Nlg1. D , Diffusion coefficients were computed for 150–2400 trajectories from 6 independent experiments. Statistical P- values: * p

    Article Snippet: To quantify the level of Nlg1 surface expression, living neurons were incubated with soluble Nrx1β-Fc (1:50 dilution in culture medium), fixed, and then stained with 1:300 goat anti-Human Fc, conjugated to cy3 using a coupling kit from GE Healthcare.

    Techniques: Expressing, Diffusion-based Assay, Labeling, Over Expression, Transfection, Fluorescence, shRNA

    Lateral diffusion is necessary for AMPAR recruitment at novel Nrx/Nlg adhesions. A , B , Rat hippocampal neurons (8 DIV) coexpressing Nlg1WT and Homer1c:GFP were incubated or not with purified Nrx1β-Fc cross-linked by Cy3 labeled secondary antibodies. The surface mobility of either AMPARs ( A , B ) or NCAM ( B ) was monitored using anti-GluA2 or anti-NCAM-conjugated Qdots, respectively. A , Representative Qdot trajectories showing the immobilization of AMPARs at Homer1c:GFP labeled synapses in untreated cells, or at Nrx1β-Fc aggregates (red pixels). B , Quantification of the global diffusion coefficients of AMPARs (450–700 trajectories) and NCAM (100–400 trajectories), and of the local AMPAR diffusion coefficient at either Homer1c:GFP spots or Nrx1β-Fc aggregates (41 and 366 trajectories, respectively). C , Neurons (8 DIV) coexpressing Nlg1 and SEP:GluA2 in control condition (left), or after surface cross-linking of SEP:GluA2 using anti-GFP antibody (right). The red traces represent GluA2-coupled Qdot trajectories. D , Quantification of the global SEP:GluA2 diffusion in control condition, or after surface cross-linking (255 and 107 trajectories, respectively, from 2 independent experiments). Statistical P- values: * p

    Journal: The Journal of Neuroscience

    Article Title: Neurexin-Neuroligin Adhesions Capture Surface-Diffusing AMPA Receptors through PSD-95 Scaffolds

    doi: 10.1523/JNEUROSCI.6439-10.2011

    Figure Lengend Snippet: Lateral diffusion is necessary for AMPAR recruitment at novel Nrx/Nlg adhesions. A , B , Rat hippocampal neurons (8 DIV) coexpressing Nlg1WT and Homer1c:GFP were incubated or not with purified Nrx1β-Fc cross-linked by Cy3 labeled secondary antibodies. The surface mobility of either AMPARs ( A , B ) or NCAM ( B ) was monitored using anti-GluA2 or anti-NCAM-conjugated Qdots, respectively. A , Representative Qdot trajectories showing the immobilization of AMPARs at Homer1c:GFP labeled synapses in untreated cells, or at Nrx1β-Fc aggregates (red pixels). B , Quantification of the global diffusion coefficients of AMPARs (450–700 trajectories) and NCAM (100–400 trajectories), and of the local AMPAR diffusion coefficient at either Homer1c:GFP spots or Nrx1β-Fc aggregates (41 and 366 trajectories, respectively). C , Neurons (8 DIV) coexpressing Nlg1 and SEP:GluA2 in control condition (left), or after surface cross-linking of SEP:GluA2 using anti-GFP antibody (right). The red traces represent GluA2-coupled Qdot trajectories. D , Quantification of the global SEP:GluA2 diffusion in control condition, or after surface cross-linking (255 and 107 trajectories, respectively, from 2 independent experiments). Statistical P- values: * p

    Article Snippet: To quantify the level of Nlg1 surface expression, living neurons were incubated with soluble Nrx1β-Fc (1:50 dilution in culture medium), fixed, and then stained with 1:300 goat anti-Human Fc, conjugated to cy3 using a coupling kit from GE Healthcare.

    Techniques: Diffusion-based Assay, Incubation, Purification, Labeling

    2-D gel image of the master gel with labeled picked proteins spots. Proteome pattern of canine liver tissue by means of 2D –DIGE. For protein analysis, proteins were labeled with Cy2, Cy3, and Cy5, separated in the first dimension using an immobilized pH gradient, and subsequently, in the second dimension, by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Protein spots were detected using a fluorescence scanner (MWM–molecular weight marker; kDA–kilodalton, NL–nonlinear).

    Journal: PLoS ONE

    Article Title: Proteomic analysis of liver tissue from dogs with chronic hepatitis

    doi: 10.1371/journal.pone.0208394

    Figure Lengend Snippet: 2-D gel image of the master gel with labeled picked proteins spots. Proteome pattern of canine liver tissue by means of 2D –DIGE. For protein analysis, proteins were labeled with Cy2, Cy3, and Cy5, separated in the first dimension using an immobilized pH gradient, and subsequently, in the second dimension, by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Protein spots were detected using a fluorescence scanner (MWM–molecular weight marker; kDA–kilodalton, NL–nonlinear).

    Article Snippet: 2- Dimensional difference gel electrophoresis Cy2-, Cy3- and Cy5-labeled samples were mixed together, and rehydration buffer was added (7 M urea, 2 M thiourea, 4% CHAPS, 0.5% pharmalytes (GE Healthcare), 40 mM dithiothreitol (DTT), and 0.002% bromophenol blue) to a final volume of 450 μl and used to rehydrate an immobilized pH gradient strip (24 cm; pH 3–10NL; GE Healthcare) by passive diffusion at 20°C for 12h.

    Techniques: Labeling, Polyacrylamide Gel Electrophoresis, Fluorescence, Molecular Weight, Marker

    Cyclic adenosine monophosphate (cAMP) levels in isolated rats cardiomyocytes, measured by enzyme immunoassay (EIA). Dose-response relationship with increasing concentrations of iloprost (1–3) in the cell culture plates. OD: optical density; 1:

    Journal: Pulmonary Circulation

    Article Title: Effects of acute intravenous iloprost on right ventricular hemodynamics in rats with chronic pulmonary hypertension

    doi: 10.1086/677358

    Figure Lengend Snippet: Cyclic adenosine monophosphate (cAMP) levels in isolated rats cardiomyocytes, measured by enzyme immunoassay (EIA). Dose-response relationship with increasing concentrations of iloprost (1–3) in the cell culture plates. OD: optical density; 1:

    Article Snippet: The rest of cell lysate was used for the adenosine monophosphate concentration measurement with a cyclic adenosine monophosphate (cAMP) enzyme-immunoassay (EIA) kit (GE Health Care–Amersham, Piscataway, NJ), according to manufacturer’s instructions.

    Techniques: Isolation, Enzyme-linked Immunosorbent Assay, Cell Culture