Article Title: Nanoemulsion-Enabled Oral Delivery of Novel Anticancer ω-3 Fatty Acid Derivatives
Figure Lengend Snippet: Final weights of excised tumors were determined at necropsy for ( a ) mice receiving increasing doses of ClFPh-CHA-loaded nanoemulsion orally and ( b ) mice receiving increasing doses of ClFPh-CHA in solution by intraperitoneal injection (32 days and 38 days of daily treatment for oral and IP administration, respectively). Values were compared to tumor weights of control groups of mice that received vehicle only (CTL). Different from control: * P
Article Snippet: Characterization of samples in terms of preparation particle size distribution (z -average and PDI) was conducted at predetermined intervals after their preparation and compared with the initial values. (d) Determination of Encapsulation Efficiency The encapsulation efficiency (EE%) of compound ClFPh-CHA into nanoemulsion was determined indirectly by the ultrafiltration method according to Equation (1). (1) EE % = ( NE Total − NE Aqueous ) NE E Total × 100 , The percentage of encapsulated substance was calculated as the difference between the total amount of compound in the formulation, which was measured after the dissolution of the nanoemulsion in methanol (NE Total), and the amount of compound present in the aqueous phase (NE Aqueous) calculated from its concentration measured in the aqueous ultrafiltrate obtained from the nanoemulsion centrifugation (5 min, 2700× g , Centrifuge 5417R, Eppendorf, Macquarie Park, NSW, Australia) using centrifugal filter tubes (Ultrafree® CL, cut-off 10,000 MW, Millipore, Burlington, MA, USA), divided by the total amount of compound in the nanoemulsion (NE Total) multiplied by 100.
Techniques: Mouse Assay, Injection, CTL Assay