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免疫组织化学染色的一抗 Primary antibodies used for immunohistochemistry

Journal: Journal of Peking University (Health Sciences)

Article Title: 肿瘤间质比评估阑尾来源腹膜假黏液瘤的临床价值

doi: 10.19723/j.issn.1671-167X.2020.02.008

Figure Lengend Snippet: 免疫组织化学染色的一抗 Primary antibodies used for immunohistochemistry

Article Snippet: B2, C1-C2, LMCP (×100) 1.2.2 免疫组织化学染色检测及结果判定 采用Envision法对30例患者进行免疫组织化学染色,包括Ki67,p53,MUC2,MUC5AC,MLH1,MLH2,MLH6,PMS2,CDX2,CK7和CK20,所有抗体均为即用型( ),按照说明书设立阳性对照,磷酸盐缓冲液代替一抗作为空白对照.结果判定:Ki67细胞核染色阳性,以50%为界值判断Ki67标记率;p53细胞核染色阳性,野生型出现强弱不等且分布不均的核染色阳性,突变型出现密集均一的核染色强阳性;MUC2和MUC5AC胞质染色阳性;错配修复(mismatch repair,MMR)基因相关蛋白MLH1,MLH2,MLH6和PMS2以正常肠黏膜,肿瘤间质细胞,炎性细胞作为内对照,细胞核染色阳性,其中任何一个缺失即为MMR基因相关蛋白缺失;CDX2细胞核染色阳性;CK7和CK20胞质和胞膜染色阳性.肿瘤细胞出现棕黄色或棕褐色颗粒定义为阳性 [ 6 , 7 ] . table ft1 table-wrap mode="anchored" t5 1 caption a7 Target Supplier Catalog number Dilution Ki67 OriGene UMAB107 Ready to use p53 OriGene DO7 Ready to use MUC2 OriGene MRQ-18 Ready to use MUC5AC OriGene MRQ-19 Ready to use MLH1 OriGene ES05 Ready to use MLH2 OriGene 25D12 Ready to use MLH6 OriGene EP49 Ready to use PMS2 OriGene EP51 Ready to use CDX2 OriGene EP25 Ready to use CK7 OriGene EP16 Ready to use CK20 OriGene EP23 Ready to use Open in a separate window 免疫组织化学染色的一抗 Primary antibodies used for immunohistochemistry 1.2.3 研究指标 (1)临床病理学特征:既往手术史,组织病理学分级,腹膜癌指数(peritoneal cancer index,PCI),细胞减灭程度(completeness of cytoreduction,CC),有无脉管癌栓,神经侵犯,淋巴结转移等;(2)免疫组织化学特征:Ki67,p53,MUC2,MUC5AC,MMR基因相关蛋白(MLH1,MLH2,MLH6和PMS2)和CDX2,CK7,CK20等;(3)生存数据:生存状态,总生存期(overall survival,OS)等.

Techniques:

免疫组织化学染色的一抗 Primary antibodies used for immunohistochemistry

Journal: Journal of Peking University (Health Sciences)

Article Title: 肿瘤间质比评估阑尾来源腹膜假黏液瘤的临床价值

doi: 10.19723/j.issn.1671-167X.2020.02.008

Figure Lengend Snippet: 免疫组织化学染色的一抗 Primary antibodies used for immunohistochemistry

Article Snippet: CK20 , OriGene , EP23 , Ready to use.

Techniques: