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Leica Microsystems eosin
Eosin, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 93/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eosin/product/Leica Microsystems
Average 93 stars, based on 109 article reviews
Price from $9.99 to $1999.99
eosin - by Bioz Stars, 2020-08
93/100 stars

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Produced:

Article Title: Fibronectin Matrix Mimetics Promote Full-Thickness Wound Repair in Diabetic Mice
Article Snippet: .. The recombinant proteins, GST/III1H,8–10, GST/III1H,8,10, GST/III1H,8RGD , and GST were produced in Escherichia coli and purified as described., Hematoxylin and eosin (H & E) were from Leica Microsystems; fibronectin polyclonal and α-tubulin monoclonal (clone DM1A) antibodies were from Sigma; horseradish peroxidase (HRP) goat anti-rabbit and goat anti-mouse antibodies were from Bio-Rad; α-smooth muscle actin (α-SMA) monoclonal antibody (clone 1A4) was from Dako; and mouse-on-mouse HRP-Polymer bundle for monoclonal antibody visualization was from Biocare Medical. .. Male C57BLKS/J (22–28 g) and C57BLKS/J-m+/+Lepr(db) mice (32–51 g) (The Jackson Laboratories) between ages 10 and 16 weeks were used.

Immunohistochemistry:

Article Title: The importance of both fibroblasts and keratinocytes in a bilayered living cellular construct used in wound healing
Article Snippet: .. Histological and immunohistochemical staining Five-micrometer formalin-fixed and paraffin-embedded sections were stained with Harris Hematoxylin (Poly Scientific, Bay Shore, NY) & Eosin Y (Leica Microsystems, Buffalo Grove, IL) for evaluation of tissue morphology at Day 20. .. Mouse anti-human cytokeratin 1/10 (AbD Serotec, Raleigh, NC) was used to identify differentiated keratinocytes.

Article Title: Contrasting effects of sunitinib within in vivo models of metastasis
Article Snippet: .. Tumour histology and immunohistochemistry For ex vivo quantification of tumour burden, formalin fixed paraffin embedded sections of mouse lung were stained with haematoxylin and eosin (H & E) and then digitally scanned using an automated scanning microscope (Ariol system, Leica Microsystems Ltd, Milton Keynes, Bucks., UK). .. Tumour burden in the scanned images was measured using Adobe Photoshop image analysis software (Adobe, Uxbridge, Middx., UK).

Microscopy:

Article Title: Contrasting effects of sunitinib within in vivo models of metastasis
Article Snippet: .. Tumour histology and immunohistochemistry For ex vivo quantification of tumour burden, formalin fixed paraffin embedded sections of mouse lung were stained with haematoxylin and eosin (H & E) and then digitally scanned using an automated scanning microscope (Ariol system, Leica Microsystems Ltd, Milton Keynes, Bucks., UK). .. Tumour burden in the scanned images was measured using Adobe Photoshop image analysis software (Adobe, Uxbridge, Middx., UK).

Purification:

Article Title: Fibronectin Matrix Mimetics Promote Full-Thickness Wound Repair in Diabetic Mice
Article Snippet: .. The recombinant proteins, GST/III1H,8–10, GST/III1H,8,10, GST/III1H,8RGD , and GST were produced in Escherichia coli and purified as described., Hematoxylin and eosin (H & E) were from Leica Microsystems; fibronectin polyclonal and α-tubulin monoclonal (clone DM1A) antibodies were from Sigma; horseradish peroxidase (HRP) goat anti-rabbit and goat anti-mouse antibodies were from Bio-Rad; α-smooth muscle actin (α-SMA) monoclonal antibody (clone 1A4) was from Dako; and mouse-on-mouse HRP-Polymer bundle for monoclonal antibody visualization was from Biocare Medical. .. Male C57BLKS/J (22–28 g) and C57BLKS/J-m+/+Lepr(db) mice (32–51 g) (The Jackson Laboratories) between ages 10 and 16 weeks were used.

Light Microscopy:

Article Title: JNK inhibitor SP600125 protects against lipopolysaccharide-induced acute lung injury via upregulation of claudin-4
Article Snippet: .. The lungs were immediately removed and post-fixed in 4% paraformaldehyde for 24 h. Paraffin-embedded sections (3 mm thick) were stained with hematoxylin and eosin (H & E) for visualization under a light microscope (magnification, ×200; Leica Microsystems, Wetzlar, Germany). .. Enzyme-linked immunosorbent assay (ELISA) The levels of claudin-4, TNF-α and IL-6 in the lung tissue samples were measured using an ELISA according to the manufacturer’s instructions (R & D Systems, Minneapolis, MN, USA).

Formalin-fixed Paraffin-Embedded:

Article Title: Contrasting effects of sunitinib within in vivo models of metastasis
Article Snippet: .. Tumour histology and immunohistochemistry For ex vivo quantification of tumour burden, formalin fixed paraffin embedded sections of mouse lung were stained with haematoxylin and eosin (H & E) and then digitally scanned using an automated scanning microscope (Ariol system, Leica Microsystems Ltd, Milton Keynes, Bucks., UK). .. Tumour burden in the scanned images was measured using Adobe Photoshop image analysis software (Adobe, Uxbridge, Middx., UK).

Inverted Microscopy:

Article Title: Decellularized Matrix from Tumorigenic Human Mesenchymal Stem Cells Promotes Neovascularization with Galectin-1 Dependent Endothelial Interaction
Article Snippet: .. Haematoxylin and Eosin Y (Bie & Berntsens Reagenslaboratorium) was used as counterstain, photomicrographs were captured under bright field illumination with an inverted microscope DM4500 B equipped with Leica DFC300 FX Digital Color Camera (Leica Microsystems A/S, Herlev, Denmark). ..

Ex Vivo:

Article Title: Contrasting effects of sunitinib within in vivo models of metastasis
Article Snippet: .. Tumour histology and immunohistochemistry For ex vivo quantification of tumour burden, formalin fixed paraffin embedded sections of mouse lung were stained with haematoxylin and eosin (H & E) and then digitally scanned using an automated scanning microscope (Ariol system, Leica Microsystems Ltd, Milton Keynes, Bucks., UK). .. Tumour burden in the scanned images was measured using Adobe Photoshop image analysis software (Adobe, Uxbridge, Middx., UK).

Staining:

Article Title: Isozyme-specific comprehensive characterization of transglutaminase-crosslinked substrates in kidney fibrosis
Article Snippet: .. Sections were also stained with hematoxylin and eosin (Leica Microsystems, Wetzlar, Germany). .. Sirius red/fast green staining Collagen fibers were detected using a Sirius Red Collagen Detection Kit (Chondrex, Redmond, USA).

Article Title: The importance of both fibroblasts and keratinocytes in a bilayered living cellular construct used in wound healing
Article Snippet: .. Histological and immunohistochemical staining Five-micrometer formalin-fixed and paraffin-embedded sections were stained with Harris Hematoxylin (Poly Scientific, Bay Shore, NY) & Eosin Y (Leica Microsystems, Buffalo Grove, IL) for evaluation of tissue morphology at Day 20. .. Mouse anti-human cytokeratin 1/10 (AbD Serotec, Raleigh, NC) was used to identify differentiated keratinocytes.

Article Title: Contrasting effects of sunitinib within in vivo models of metastasis
Article Snippet: .. Tumour histology and immunohistochemistry For ex vivo quantification of tumour burden, formalin fixed paraffin embedded sections of mouse lung were stained with haematoxylin and eosin (H & E) and then digitally scanned using an automated scanning microscope (Ariol system, Leica Microsystems Ltd, Milton Keynes, Bucks., UK). .. Tumour burden in the scanned images was measured using Adobe Photoshop image analysis software (Adobe, Uxbridge, Middx., UK).

Article Title: N-acetylcysteine Decreases Fibrosis and Increases Force-Generating Capacity of mdx Diaphragm
Article Snippet: .. Histological Analysis To examine putative inflammatory cell infiltration of muscle fibres, tissue sections were stained with haematoxylin and eosin (H & E) using an autostainer (Leica ST5010 Autostainer XL, Leica Microsystems, Nussloch, Germany). .. For collagen staining, picro-sirius red (Leica Biosystems, Wetzlar, Germany) staining was completed.

Article Title: p75 neurotrophin receptor: A potential surface marker of tongue squamous cell carcinoma stem cells
Article Snippet: .. Subsequently, 5-µm serial sections were prepared for histological hematoxylin and eosin (H & E) staining (LEICA SM 2010R; Leica Microsystems GmbH, Wetzlar, Germany). .. Histological examination and image analysis H & E staining was performed to investigate the morphology.

Article Title: JNK inhibitor SP600125 protects against lipopolysaccharide-induced acute lung injury via upregulation of claudin-4
Article Snippet: .. The lungs were immediately removed and post-fixed in 4% paraformaldehyde for 24 h. Paraffin-embedded sections (3 mm thick) were stained with hematoxylin and eosin (H & E) for visualization under a light microscope (magnification, ×200; Leica Microsystems, Wetzlar, Germany). .. Enzyme-linked immunosorbent assay (ELISA) The levels of claudin-4, TNF-α and IL-6 in the lung tissue samples were measured using an ELISA according to the manufacturer’s instructions (R & D Systems, Minneapolis, MN, USA).

Recombinant:

Article Title: Fibronectin Matrix Mimetics Promote Full-Thickness Wound Repair in Diabetic Mice
Article Snippet: .. The recombinant proteins, GST/III1H,8–10, GST/III1H,8,10, GST/III1H,8RGD , and GST were produced in Escherichia coli and purified as described., Hematoxylin and eosin (H & E) were from Leica Microsystems; fibronectin polyclonal and α-tubulin monoclonal (clone DM1A) antibodies were from Sigma; horseradish peroxidase (HRP) goat anti-rabbit and goat anti-mouse antibodies were from Bio-Rad; α-smooth muscle actin (α-SMA) monoclonal antibody (clone 1A4) was from Dako; and mouse-on-mouse HRP-Polymer bundle for monoclonal antibody visualization was from Biocare Medical. .. Male C57BLKS/J (22–28 g) and C57BLKS/J-m+/+Lepr(db) mice (32–51 g) (The Jackson Laboratories) between ages 10 and 16 weeks were used.

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  • 92
    Leica Microsystems eosin y
    Histological and immunohistochemical staining shows differences between BLCC, Fibroblast matrix, and Epithelium only constructs. Hematoxylin and <t>eosin</t> (H E) (A–C); Cytokeratin 1/10 (D–F); Collagen type IV (G–I); and Ki67 (J–L) show functional and morphological differences between constructs. Differentiated keratinocytes were only identified in the BLCC (D–F). Collagen type IV, indicating basement membrane deposition, and Ki67 immunostaining for proliferating cells were found at the interface of the dermal and epidermal layers in the BLCC, but not in Fibroblast matrix or Epithelium only constructs (G–I, J–L). Dotted lines in (A), (D), (G), and (J) demarcate the dermal–epidermal interface in the BLCC. Day 20 results shown. Each image is representative of n = 3 samples per group. BLCC, bilayered living cellular construct.
    Eosin Y, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eosin y/product/Leica Microsystems
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eosin y - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Leica Microsystems eosin staining clear cell renal cell carcinoma tissues
    Hsa_circ_001895 promoted <t>clear</t> <t>cell</t> <t>renal</t> cell <t>carcinoma</t> (ccRCC) proliferation. A, Expression of hsa_circ_001895 in cytoplasm or nucleus of 786‐O and A498 cells detected by qRT‐PCR. B, Subcellular localization of hsa_circ_001895 in 786‐O and A498 cells by RNA‐FISH. C, Transfection efficiency of sh‐hsa_circ_001895, sh‐CTBP1, pcDNA‐hsa_circ_001895 and pcDNA‐CTBP1 in 786‐O and A498 cells detected by qRT‐PCR. D, Influence of hsa_circ_001895 on cell viability of 786‐O and A498 cells detected by CCK‐8. E, Influence of hsa_circ_001895 on cell proliferation of 786‐O and A498 cells detected by colony formation assay. F, Influence of hsa_circ_001895 on cell proliferation of 786‐O and A498 cells detected by 5‐ethynyl‐2′‐deoxyuridine (EdU) <t>staining</t> assay. G, Influence of hsa_circ_001895 on cell cycle of 786‐O and A498 cells detected by flow cytometry. *, **sh‐hsa_circ_001895 vs sh‐NC or pcDNA‐hsa_circ_001895 vs EV, P
    Eosin Staining Clear Cell Renal Cell Carcinoma Tissues, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eosin staining clear cell renal cell carcinoma tissues/product/Leica Microsystems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eosin staining clear cell renal cell carcinoma tissues - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Leica Microsystems eosin h e stained
    Hsa_circ_001895 promoted <t>clear</t> <t>cell</t> <t>renal</t> cell <t>carcinoma</t> (ccRCC) proliferation. A, Expression of hsa_circ_001895 in cytoplasm or nucleus of 786‐O and A498 cells detected by qRT‐PCR. B, Subcellular localization of hsa_circ_001895 in 786‐O and A498 cells by RNA‐FISH. C, Transfection efficiency of sh‐hsa_circ_001895, sh‐CTBP1, pcDNA‐hsa_circ_001895 and pcDNA‐CTBP1 in 786‐O and A498 cells detected by qRT‐PCR. D, Influence of hsa_circ_001895 on cell viability of 786‐O and A498 cells detected by CCK‐8. E, Influence of hsa_circ_001895 on cell proliferation of 786‐O and A498 cells detected by colony formation assay. F, Influence of hsa_circ_001895 on cell proliferation of 786‐O and A498 cells detected by 5‐ethynyl‐2′‐deoxyuridine (EdU) <t>staining</t> assay. G, Influence of hsa_circ_001895 on cell cycle of 786‐O and A498 cells detected by flow cytometry. *, **sh‐hsa_circ_001895 vs sh‐NC or pcDNA‐hsa_circ_001895 vs EV, P
    Eosin H E Stained, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eosin h e stained/product/Leica Microsystems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eosin h e stained - by Bioz Stars, 2020-08
    93/100 stars
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    Image Search Results


    Histological and immunohistochemical staining shows differences between BLCC, Fibroblast matrix, and Epithelium only constructs. Hematoxylin and eosin (H E) (A–C); Cytokeratin 1/10 (D–F); Collagen type IV (G–I); and Ki67 (J–L) show functional and morphological differences between constructs. Differentiated keratinocytes were only identified in the BLCC (D–F). Collagen type IV, indicating basement membrane deposition, and Ki67 immunostaining for proliferating cells were found at the interface of the dermal and epidermal layers in the BLCC, but not in Fibroblast matrix or Epithelium only constructs (G–I, J–L). Dotted lines in (A), (D), (G), and (J) demarcate the dermal–epidermal interface in the BLCC. Day 20 results shown. Each image is representative of n = 3 samples per group. BLCC, bilayered living cellular construct.

    Journal: Wound Repair and Regeneration

    Article Title: The importance of both fibroblasts and keratinocytes in a bilayered living cellular construct used in wound healing

    doi: 10.1111/wrr.12154

    Figure Lengend Snippet: Histological and immunohistochemical staining shows differences between BLCC, Fibroblast matrix, and Epithelium only constructs. Hematoxylin and eosin (H E) (A–C); Cytokeratin 1/10 (D–F); Collagen type IV (G–I); and Ki67 (J–L) show functional and morphological differences between constructs. Differentiated keratinocytes were only identified in the BLCC (D–F). Collagen type IV, indicating basement membrane deposition, and Ki67 immunostaining for proliferating cells were found at the interface of the dermal and epidermal layers in the BLCC, but not in Fibroblast matrix or Epithelium only constructs (G–I, J–L). Dotted lines in (A), (D), (G), and (J) demarcate the dermal–epidermal interface in the BLCC. Day 20 results shown. Each image is representative of n = 3 samples per group. BLCC, bilayered living cellular construct.

    Article Snippet: Histological and immunohistochemical staining Five-micrometer formalin-fixed and paraffin-embedded sections were stained with Harris Hematoxylin (Poly Scientific, Bay Shore, NY) & Eosin Y (Leica Microsystems, Buffalo Grove, IL) for evaluation of tissue morphology at Day 20.

    Techniques: Immunohistochemistry, Staining, Construct, Functional Assay, Immunostaining

    Histological sections of MESA plugs after 7 days in vivo . A : Photomicrograph of a MESA implant showing a 1 cm diameter matrigel plug with a centrally implanted sponge (arrow, “S”). B : Haemotoxylin and Eosin stain of a sponge loaded with ECBM-MV2 medium. C , D : Histological sections stained with murine specific anti-CD34 antibody visualized by brown chromogen diaminobenzidine. C : Field of view of matrigel adjacent to sponge seeded with -BC8 cells. D : Field of view of matrigel adjacent to sponge seeded with -BD11 cells. E : Chalkley count quantification of vasculature adjacent to the Matrigel® embedded sponge. *P

    Journal: PLoS ONE

    Article Title: Decellularized Matrix from Tumorigenic Human Mesenchymal Stem Cells Promotes Neovascularization with Galectin-1 Dependent Endothelial Interaction

    doi: 10.1371/journal.pone.0021888

    Figure Lengend Snippet: Histological sections of MESA plugs after 7 days in vivo . A : Photomicrograph of a MESA implant showing a 1 cm diameter matrigel plug with a centrally implanted sponge (arrow, “S”). B : Haemotoxylin and Eosin stain of a sponge loaded with ECBM-MV2 medium. C , D : Histological sections stained with murine specific anti-CD34 antibody visualized by brown chromogen diaminobenzidine. C : Field of view of matrigel adjacent to sponge seeded with -BC8 cells. D : Field of view of matrigel adjacent to sponge seeded with -BD11 cells. E : Chalkley count quantification of vasculature adjacent to the Matrigel® embedded sponge. *P

    Article Snippet: Haematoxylin and Eosin Y (Bie & Berntsens Reagenslaboratorium) was used as counterstain, photomicrographs were captured under bright field illumination with an inverted microscope DM4500 B equipped with Leica DFC300 FX Digital Color Camera (Leica Microsystems A/S, Herlev, Denmark).

    Techniques: In Vivo, Staining

    Hsa_circ_001895 promoted clear cell renal cell carcinoma (ccRCC) proliferation. A, Expression of hsa_circ_001895 in cytoplasm or nucleus of 786‐O and A498 cells detected by qRT‐PCR. B, Subcellular localization of hsa_circ_001895 in 786‐O and A498 cells by RNA‐FISH. C, Transfection efficiency of sh‐hsa_circ_001895, sh‐CTBP1, pcDNA‐hsa_circ_001895 and pcDNA‐CTBP1 in 786‐O and A498 cells detected by qRT‐PCR. D, Influence of hsa_circ_001895 on cell viability of 786‐O and A498 cells detected by CCK‐8. E, Influence of hsa_circ_001895 on cell proliferation of 786‐O and A498 cells detected by colony formation assay. F, Influence of hsa_circ_001895 on cell proliferation of 786‐O and A498 cells detected by 5‐ethynyl‐2′‐deoxyuridine (EdU) staining assay. G, Influence of hsa_circ_001895 on cell cycle of 786‐O and A498 cells detected by flow cytometry. *, **sh‐hsa_circ_001895 vs sh‐NC or pcDNA‐hsa_circ_001895 vs EV, P

    Journal: Cancer Science

    Article Title: Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12. Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

    doi: 10.1111/cas.14261

    Figure Lengend Snippet: Hsa_circ_001895 promoted clear cell renal cell carcinoma (ccRCC) proliferation. A, Expression of hsa_circ_001895 in cytoplasm or nucleus of 786‐O and A498 cells detected by qRT‐PCR. B, Subcellular localization of hsa_circ_001895 in 786‐O and A498 cells by RNA‐FISH. C, Transfection efficiency of sh‐hsa_circ_001895, sh‐CTBP1, pcDNA‐hsa_circ_001895 and pcDNA‐CTBP1 in 786‐O and A498 cells detected by qRT‐PCR. D, Influence of hsa_circ_001895 on cell viability of 786‐O and A498 cells detected by CCK‐8. E, Influence of hsa_circ_001895 on cell proliferation of 786‐O and A498 cells detected by colony formation assay. F, Influence of hsa_circ_001895 on cell proliferation of 786‐O and A498 cells detected by 5‐ethynyl‐2′‐deoxyuridine (EdU) staining assay. G, Influence of hsa_circ_001895 on cell cycle of 786‐O and A498 cells detected by flow cytometry. *, **sh‐hsa_circ_001895 vs sh‐NC or pcDNA‐hsa_circ_001895 vs EV, P

    Article Snippet: 2.14 Hematoxylin and eosin staining Clear cell renal cell carcinoma tissues from mice were fixed in 4% paraformaldehyde and then processed by a paraffin tissue‐processing machine, cut into 7‐μm sections using a RM2245 microtome (Leica Microsystems).

    Techniques: Expressing, Quantitative RT-PCR, Fluorescence In Situ Hybridization, Transfection, CCK-8 Assay, Colony Assay, Staining, Flow Cytometry

    Circular RNA (circRNA) hsa_circ_001895 was upregulated in both clear cell renal cell carcinoma (ccRCC) tissues and cell lines. A, Heat map shows dysregulated circRNAs between ccRCC tissues (T) and adjacent noncancer tissues (N). B, Expression of circRNA hsa_circ_001895 in ccRCC tissues and adjacent noncancer tissues detected by qRT‐PCR (N = 60). C, In situ hybridization analysis of hsa_circ_001895 expression in ccRCC tissues with different TNM stage. D, Overall survival analysis of ccRCC patients with high hsa_circ_001895 expression and low levels of hsa_circ_001895. E, Expression of hsa_circ_001895 in human ccRCC cell lines (786‐O, A498, OS‐RC‐2, 769‐P and ACHN) and HK2 detected by qRT‐PCR. F, Hsa_circ_001895 was resistant to RNase R digestion compared to linear CTBP1. **Tumor vs normal tissues, ccRCC cell lines vs HK2, RNase R vs MOCK, P

    Journal: Cancer Science

    Article Title: Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12. Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

    doi: 10.1111/cas.14261

    Figure Lengend Snippet: Circular RNA (circRNA) hsa_circ_001895 was upregulated in both clear cell renal cell carcinoma (ccRCC) tissues and cell lines. A, Heat map shows dysregulated circRNAs between ccRCC tissues (T) and adjacent noncancer tissues (N). B, Expression of circRNA hsa_circ_001895 in ccRCC tissues and adjacent noncancer tissues detected by qRT‐PCR (N = 60). C, In situ hybridization analysis of hsa_circ_001895 expression in ccRCC tissues with different TNM stage. D, Overall survival analysis of ccRCC patients with high hsa_circ_001895 expression and low levels of hsa_circ_001895. E, Expression of hsa_circ_001895 in human ccRCC cell lines (786‐O, A498, OS‐RC‐2, 769‐P and ACHN) and HK2 detected by qRT‐PCR. F, Hsa_circ_001895 was resistant to RNase R digestion compared to linear CTBP1. **Tumor vs normal tissues, ccRCC cell lines vs HK2, RNase R vs MOCK, P

    Article Snippet: 2.14 Hematoxylin and eosin staining Clear cell renal cell carcinoma tissues from mice were fixed in 4% paraformaldehyde and then processed by a paraffin tissue‐processing machine, cut into 7‐μm sections using a RM2245 microtome (Leica Microsystems).

    Techniques: Expressing, Quantitative RT-PCR, In Situ Hybridization

    SOX12 was a direct target of microRNA (miR)‐296‐5p. A, Potential miR‐296‐5p binding targets predicted by TargetScan, miRDB, miRTarBase, miRWalk and miRTargetLink Human. B, Relative intensity of RNF44, SOX12 and NFIC in HEK293 cells. C, Influence of hsa_circ_001895 on mRNA expression of RNF44, SOX12 and nuclear factor I‐C (NFIC) in 786‐O and A498 cells detected by qRT‐PCR. D, Influence of hsa_circ_001895 on protein expression of RNF44, SOX12 and NFIC in 786‐O and A498 cells detected by qRT‐PCR. E, Wild‐type and mutant binding sites of miR‐296‐5p in SOX12. F, Influence of miR‐296‐5p mimics on luciferase activities of pmirGLO‐wt‐SOX12 or pmirGLO‐mut‐SOX12 in 786‐O and A498 cells detected by qRT‐PCR. G, Transfection efficiency of miR‐296‐5p mimics or inhibitor in 786‐O and A498 cells detected by qRT‐PCR. H, Influence of miR‐296‐5p on protein expression of SOX12 in 786‐O and A498 cells detected by western blot. I, mRNA expression of SOX12 in clear cell renal cell carcinoma (ccRCC) tissues and adjacent noncancer tissues detected by qRT‐PCR (N = 60). J, In situ hybridization (ISH) analysis of SOX12 expression in ccRCC tissues with different TNM stage. K, Negative correlation between miR‐296‐5p and SOX12 in ccRCC patients. Positive correlation between hsa_circ_001895 and SOX12 in ccRCC patients. L, Protein expression of SOX12 in ccRCC tissues and adjacent noncancer tissues detected by western blot. **sh‐hsa_circ_001895 vs sh‐NC, miR‐296‐5p mimics vs miR‐NC, miR‐296‐5p inhibitor vs inh NC, Tumor vs Normal tissues, P

    Journal: Cancer Science

    Article Title: Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12. Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

    doi: 10.1111/cas.14261

    Figure Lengend Snippet: SOX12 was a direct target of microRNA (miR)‐296‐5p. A, Potential miR‐296‐5p binding targets predicted by TargetScan, miRDB, miRTarBase, miRWalk and miRTargetLink Human. B, Relative intensity of RNF44, SOX12 and NFIC in HEK293 cells. C, Influence of hsa_circ_001895 on mRNA expression of RNF44, SOX12 and nuclear factor I‐C (NFIC) in 786‐O and A498 cells detected by qRT‐PCR. D, Influence of hsa_circ_001895 on protein expression of RNF44, SOX12 and NFIC in 786‐O and A498 cells detected by qRT‐PCR. E, Wild‐type and mutant binding sites of miR‐296‐5p in SOX12. F, Influence of miR‐296‐5p mimics on luciferase activities of pmirGLO‐wt‐SOX12 or pmirGLO‐mut‐SOX12 in 786‐O and A498 cells detected by qRT‐PCR. G, Transfection efficiency of miR‐296‐5p mimics or inhibitor in 786‐O and A498 cells detected by qRT‐PCR. H, Influence of miR‐296‐5p on protein expression of SOX12 in 786‐O and A498 cells detected by western blot. I, mRNA expression of SOX12 in clear cell renal cell carcinoma (ccRCC) tissues and adjacent noncancer tissues detected by qRT‐PCR (N = 60). J, In situ hybridization (ISH) analysis of SOX12 expression in ccRCC tissues with different TNM stage. K, Negative correlation between miR‐296‐5p and SOX12 in ccRCC patients. Positive correlation between hsa_circ_001895 and SOX12 in ccRCC patients. L, Protein expression of SOX12 in ccRCC tissues and adjacent noncancer tissues detected by western blot. **sh‐hsa_circ_001895 vs sh‐NC, miR‐296‐5p mimics vs miR‐NC, miR‐296‐5p inhibitor vs inh NC, Tumor vs Normal tissues, P

    Article Snippet: 2.14 Hematoxylin and eosin staining Clear cell renal cell carcinoma tissues from mice were fixed in 4% paraformaldehyde and then processed by a paraffin tissue‐processing machine, cut into 7‐μm sections using a RM2245 microtome (Leica Microsystems).

    Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Mutagenesis, Luciferase, Transfection, Western Blot, In Situ Hybridization

    Negative correlation between hsa_circ_001895 and microRNA (miR)‐296‐5p. A, Potential binding targets of hsa_circ_001895 predicted by CircInteractome (circular RNA interactome) and starBase. B, Relative intensity of miR‐516a‐5p, miR‐520h and miR‐296‐5p in HEK293 cells. C, Influence of hsa_circ_001895 on miR‐516a‐5p, miR‐520h and miR‐296‐5p expression in 786‐O and A498 cells detected by qRT‐PCR. D, Subcellular localization of hsa_circ_001895 and miR‐296‐5p in 786‐O and A498 cells by RNA‐FISH. E, Wild‐type and mutant binding sites of miR‐296‐5p in hsa_circ_001895. F, Influence of miR‐296‐5p mimics on luciferase activities of pmirGLO‐wt‐hsa_circ_001895 or pmirGLO‐mut‐hsa_circ_001895 in 786‐O and A498 cells detected by qRT‐PCR. G, Enrichment of hsa_circ_001895 and miR‐296‐5p in Ago2‐containing beads of 786‐O and A498 cells. H, Expression of miR‐296‐5p in clear cell renal cell carcinoma (ccRCC) tissues and adjacent noncancer tissues detected by qRT‐PCR (N = 60). I, Negative correlation between miR‐296‐5p and hsa_circ_001895 in ccRCC patients. **sh‐hsa_circ_001895 vs sh‐NC, miR‐296‐5p mimics vs miR‐NC, Input vs Anti‐IgG, Tumor vs Normal tissues, P

    Journal: Cancer Science

    Article Title: Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12. Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

    doi: 10.1111/cas.14261

    Figure Lengend Snippet: Negative correlation between hsa_circ_001895 and microRNA (miR)‐296‐5p. A, Potential binding targets of hsa_circ_001895 predicted by CircInteractome (circular RNA interactome) and starBase. B, Relative intensity of miR‐516a‐5p, miR‐520h and miR‐296‐5p in HEK293 cells. C, Influence of hsa_circ_001895 on miR‐516a‐5p, miR‐520h and miR‐296‐5p expression in 786‐O and A498 cells detected by qRT‐PCR. D, Subcellular localization of hsa_circ_001895 and miR‐296‐5p in 786‐O and A498 cells by RNA‐FISH. E, Wild‐type and mutant binding sites of miR‐296‐5p in hsa_circ_001895. F, Influence of miR‐296‐5p mimics on luciferase activities of pmirGLO‐wt‐hsa_circ_001895 or pmirGLO‐mut‐hsa_circ_001895 in 786‐O and A498 cells detected by qRT‐PCR. G, Enrichment of hsa_circ_001895 and miR‐296‐5p in Ago2‐containing beads of 786‐O and A498 cells. H, Expression of miR‐296‐5p in clear cell renal cell carcinoma (ccRCC) tissues and adjacent noncancer tissues detected by qRT‐PCR (N = 60). I, Negative correlation between miR‐296‐5p and hsa_circ_001895 in ccRCC patients. **sh‐hsa_circ_001895 vs sh‐NC, miR‐296‐5p mimics vs miR‐NC, Input vs Anti‐IgG, Tumor vs Normal tissues, P

    Article Snippet: 2.14 Hematoxylin and eosin staining Clear cell renal cell carcinoma tissues from mice were fixed in 4% paraformaldehyde and then processed by a paraffin tissue‐processing machine, cut into 7‐μm sections using a RM2245 microtome (Leica Microsystems).

    Techniques: Binding Assay, Expressing, Quantitative RT-PCR, Fluorescence In Situ Hybridization, Mutagenesis, Luciferase

    Hsa_circ_001895 knockdown inhibited in vivo clear cell renal cell carcinoma (ccRCC) tumor growth. A, Influence of sh‐hsa_circ_001895 on hsa_circ_001895 and microRNA (miR)‐296‐5p expression in mice intratumorally injected with lentiviral vector with hsa_circ_001895 knockdown or the negative control (sh‐NC). B, Effect of sh‐hsa_circ_001895 on ccRCC tumor growth in xenograft tumor mice. C, Influence of sh‐hsa_circ_001895 on tumor volume and weight. D, H E staining shows morphological features of ccRCC tissues, and immunohistochemical staining was used to determine expression of SOX12, Ki‐67, E‐cadherin, N‐cadherin and Cleaved caspase 3 affected by sh‐hsa_circ_001895. Black bar, 200 μm. *, **sh‐hsa_circ_001895 vs sh‐NC, P

    Journal: Cancer Science

    Article Title: Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12. Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

    doi: 10.1111/cas.14261

    Figure Lengend Snippet: Hsa_circ_001895 knockdown inhibited in vivo clear cell renal cell carcinoma (ccRCC) tumor growth. A, Influence of sh‐hsa_circ_001895 on hsa_circ_001895 and microRNA (miR)‐296‐5p expression in mice intratumorally injected with lentiviral vector with hsa_circ_001895 knockdown or the negative control (sh‐NC). B, Effect of sh‐hsa_circ_001895 on ccRCC tumor growth in xenograft tumor mice. C, Influence of sh‐hsa_circ_001895 on tumor volume and weight. D, H E staining shows morphological features of ccRCC tissues, and immunohistochemical staining was used to determine expression of SOX12, Ki‐67, E‐cadherin, N‐cadherin and Cleaved caspase 3 affected by sh‐hsa_circ_001895. Black bar, 200 μm. *, **sh‐hsa_circ_001895 vs sh‐NC, P

    Article Snippet: 2.14 Hematoxylin and eosin staining Clear cell renal cell carcinoma tissues from mice were fixed in 4% paraformaldehyde and then processed by a paraffin tissue‐processing machine, cut into 7‐μm sections using a RM2245 microtome (Leica Microsystems).

    Techniques: In Vivo, Expressing, Mouse Assay, Injection, Plasmid Preparation, Negative Control, Staining, Immunohistochemistry

    Hsa_circ_001895 knockdown inhibited clear cell renal cell carcinoma (ccRCC) proliferation by sponging microRNA (miR)‐296‐5p. A, Influence of hsa_circ_001895 and miR‐296‐5p on cell viability of 786‐O and A498 cells detected by CCK‐8. B, Influence of hsa_circ_001895 and miR‐296‐5p on cell proliferation of 786‐O and A498 cells detected by colony formation assay. C, Influence of hsa_circ_001895 and miR‐296‐5p on cell proliferation of 786‐O and A498 cells detected by 5‐ethynyl‐2′‐deoxyuridine (EdU) staining assay. D, Influence of hsa_circ_001895 and miR‐296‐5p on cell cycle of 786‐O and A498 cells detected by flow cytometry. *, **sh‐hsa_circ_001895 + inh NC vs sh‐NC + inh NC, P

    Journal: Cancer Science

    Article Title: Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12. Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

    doi: 10.1111/cas.14261

    Figure Lengend Snippet: Hsa_circ_001895 knockdown inhibited clear cell renal cell carcinoma (ccRCC) proliferation by sponging microRNA (miR)‐296‐5p. A, Influence of hsa_circ_001895 and miR‐296‐5p on cell viability of 786‐O and A498 cells detected by CCK‐8. B, Influence of hsa_circ_001895 and miR‐296‐5p on cell proliferation of 786‐O and A498 cells detected by colony formation assay. C, Influence of hsa_circ_001895 and miR‐296‐5p on cell proliferation of 786‐O and A498 cells detected by 5‐ethynyl‐2′‐deoxyuridine (EdU) staining assay. D, Influence of hsa_circ_001895 and miR‐296‐5p on cell cycle of 786‐O and A498 cells detected by flow cytometry. *, **sh‐hsa_circ_001895 + inh NC vs sh‐NC + inh NC, P

    Article Snippet: 2.14 Hematoxylin and eosin staining Clear cell renal cell carcinoma tissues from mice were fixed in 4% paraformaldehyde and then processed by a paraffin tissue‐processing machine, cut into 7‐μm sections using a RM2245 microtome (Leica Microsystems).

    Techniques: CCK-8 Assay, Colony Assay, Staining, Flow Cytometry