Journal: Advanced Science

Article Title: Dysmyelination by Oligodendrocyte‐Specific Ablation of Ninj2 Contributes to Depressive‐Like Behaviors

doi: 10.1002/advs.202103065

Figure Lengend Snippet: Ccl2 release from necroptotic oligodendrocytes changes the activities of primary pyramidal neurons. A) Expression of inflammatory cytokines in oligodendrocytes derived from WT or Olig1 cre/+ ;Ninj2 fl/fl (cKO) mice. n = 3 independent experiments. B) Ccl2 levels in oligodendrocytes cell culture supernatant derived from WT or cKO mice was determined by ELISA. n = 4 independent experiments. C) Ccl2 mRNA levels in primary oligodendrocytes from WT or cKO mice treated with vehicle or Nec‐1s (1 µ m ). n = 3 independent experiments. D) Ccl2 levels in oligodendrocytes cell culture supernatant derived from WT or Olig1 cre/+ ;Ninj2 fl/fl (cKO) mice treated with vehicle or Nec‐1s (1 µ m ) was determined by ELISA. n = 3 independent experiments. E,F) Whole‐cell patch‐clamp recording was performed to show the representative traces and quantification of frequencies and amplitudes of mEPSCs or mIPSCs from primary culture pyramidal neurons treated with vehicle or Ccl2 (100 ng mL −1 ) for 48 h. ( n = 16 neurons for each group). All the quantification data are presented as mean ± SEM, p ‐values are calculated using two‐tailed unpaired Student's t ‐test (A,B,E,F) or two‐way ANOVA with Tukey's multiple comparisons test (C,D), C (interaction [ F 1,8 = 13.76, p = 0.006]), D (interaction [ F 1,8 = 97.49, p < 0.0001]), * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Ccl2 levels in cell culture supernatant were measured using mouse Ccl2 enzyme linked immunosorbent assay (ELISA) Kits (Boster, Cat# EK0568) according to the manufacturer's instructions.

Techniques: Expressing, Derivative Assay, Cell Culture, Enzyme-linked Immunosorbent Assay, Patch Clamp, Two Tailed Test