enscs  (Qiagen)

 
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    Name:
    RNeasy Plus Micro Kit
    Description:
    For purification of up to 45 µg total RNA from cells tissues using gDNA Eliminator columns Kit contents Qiagen RNeasy Plus Micro Kit 50 preps 14L Elution Volume 5mg Tissue Amount Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes RNeasy MinElute Spin Columns gDNA Eliminator Spin Columns Collection Tubes Carrier RNA RNase free Water and Buffers Benefits Unique gDNA Eliminator columns avoid the need for DNase Efficient removal of genomic DNA Highly reproducible yields of RNA in minutes High performance RNA for sensitive application
    Catalog Number:
    74034
    Price:
    436
    Category:
    RNeasy Plus Micro Kit
    Buy from Supplier


    Structured Review

    Qiagen enscs
    RNeasy Plus Micro Kit
    For purification of up to 45 µg total RNA from cells tissues using gDNA Eliminator columns Kit contents Qiagen RNeasy Plus Micro Kit 50 preps 14L Elution Volume 5mg Tissue Amount Tissue Cells Sample Total RNA Purification Spin Column Format Silica Technology Ideal for Northern Dot and Slot Blotting End point RT PCR Quantitative Real time RT PCR Includes RNeasy MinElute Spin Columns gDNA Eliminator Spin Columns Collection Tubes Carrier RNA RNase free Water and Buffers Benefits Unique gDNA Eliminator columns avoid the need for DNase Efficient removal of genomic DNA Highly reproducible yields of RNA in minutes High performance RNA for sensitive application
    https://www.bioz.com/result/enscs/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    enscs - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells"

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-25921-8

    Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P
    Figure Legend Snippet: Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Techniques Used: Expressing, Over Expression, RNA Sequencing Assay, Plasmid Preparation

    Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.
    Figure Legend Snippet: Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Techniques Used: Expressing, Over Expression, RNA Sequencing Assay

    2) Product Images from "Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells"

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-25921-8

    Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P
    Figure Legend Snippet: Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Techniques Used: Expressing, Over Expression, RNA Sequencing Assay, Plasmid Preparation

    Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.
    Figure Legend Snippet: Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Techniques Used: Expressing, Over Expression, RNA Sequencing Assay

    Related Articles

    RNA Sequencing Assay:

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
    Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

    High Throughput Screening Assay:

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
    Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

    Isolation:

    Article Title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability
    Article Snippet: .. Real time quantitative PCR Total RNA was extracted from FAC sorted GFP+ cells using RNA column-based isolation kits, RNeasy® Micro kit (Qiagen). .. Concentration and integrity of the RNA extracted from FAC sorted cells were determined with an Agilent 2100 Bioanalyzer and by use of the Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
    Article Snippet: .. The RNAqueous Micro Total RNA Isolation Kit from Ambion and the RNeasy Plus Micro Kit from Qiagen are specifically designed for purification of total RNA from a small amount of cells ( < 200,000 cells). .. In both cases, only longer RNA fragments ( > 200 nucleotides) are purified, although the workflow can be modified to isolate small RNAs such as microRNAs, 5,8S rRNA, 5S RNA, tRNA’s,… (Additional file : Figure S1).

    Article Title: Cholinergic signals from the CNS regulate G-CSF-mediated HSC mobilization from bone marrow via a glucocorticoid signaling relay
    Article Snippet: .. RNA for microarray analysis was isolated by sorting HSC (Lineage− Sca1+ cKit+ Flt3− directly into RLT buffer from the RNeasy Plus Micro kit (Qiagen) according to the manufacturercs instructions. .. Amplification and subsequent microarray was performed by the Genomics Core Facility at Albert Einstein College of Medicine using the One-Direct RNA amplification system (NuGEN) and GeneChip mouse gene 2.0 ST array (Affymetrix).

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
    Article Snippet: .. Based on different quality parameters and comparison of various experimental set-ups, we recommend using the RNeasy Plus Micro Kit for RNA isolation of FACS sorted zebrafish cells. .. The RNA purification protocol is fast (only 20–30 min), easy and consistently delivers high-quality RNA samples.

    Article Title: Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-related Macular Degeneration
    Article Snippet: .. To confirm that no residual RPE donor cells contaminated the hiPSC cultures, total RNA was isolated (Cat. # 74034; Qiagen) from each hiPSC line, cDNA was reverse transcribed (Cat. # 4387406; Thermo Fisher) and qPCR performed using primers specific for the RPE gene RPE65 ( ). .. hiPSC lines were grown on irradiated mouse embryonic feeders (Cat. # GSE-6301G; GlobalStem) in serum-free medium supplemented with 4ng/ml FGF2 (Cat. # 233-FB; R & D systems).

    Purification:

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
    Article Snippet: .. The RNAqueous Micro Total RNA Isolation Kit from Ambion and the RNeasy Plus Micro Kit from Qiagen are specifically designed for purification of total RNA from a small amount of cells ( < 200,000 cells). .. In both cases, only longer RNA fragments ( > 200 nucleotides) are purified, although the workflow can be modified to isolate small RNAs such as microRNAs, 5,8S rRNA, 5S RNA, tRNA’s,… (Additional file : Figure S1).

    Real-time Polymerase Chain Reaction:

    Article Title: Spinal neurons require Islet1 for subtype-specific differentiation of electrical excitability
    Article Snippet: .. Real time quantitative PCR Total RNA was extracted from FAC sorted GFP+ cells using RNA column-based isolation kits, RNeasy® Micro kit (Qiagen). .. Concentration and integrity of the RNA extracted from FAC sorted cells were determined with an Agilent 2100 Bioanalyzer and by use of the Agilent RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA).

    Article Title: Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-related Macular Degeneration
    Article Snippet: .. To confirm that no residual RPE donor cells contaminated the hiPSC cultures, total RNA was isolated (Cat. # 74034; Qiagen) from each hiPSC line, cDNA was reverse transcribed (Cat. # 4387406; Thermo Fisher) and qPCR performed using primers specific for the RPE gene RPE65 ( ). .. hiPSC lines were grown on irradiated mouse embryonic feeders (Cat. # GSE-6301G; GlobalStem) in serum-free medium supplemented with 4ng/ml FGF2 (Cat. # 233-FB; R & D systems).

    Microarray:

    Article Title: Cholinergic signals from the CNS regulate G-CSF-mediated HSC mobilization from bone marrow via a glucocorticoid signaling relay
    Article Snippet: .. RNA for microarray analysis was isolated by sorting HSC (Lineage− Sca1+ cKit+ Flt3− directly into RLT buffer from the RNeasy Plus Micro kit (Qiagen) according to the manufacturercs instructions. .. Amplification and subsequent microarray was performed by the Genomics Core Facility at Albert Einstein College of Medicine using the One-Direct RNA amplification system (NuGEN) and GeneChip mouse gene 2.0 ST array (Affymetrix).

    other:

    Article Title: Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease
    Article Snippet: To remove genomic DNA from the Zymo processed sample, the eluted RNA was resuspended in 350 µl RLT buffer and processed using the RNeasy Plus Micro kit and eluted in 16 µl nuclease-free water.

    Sequencing:

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells
    Article Snippet: .. High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples. .. Sequencing libraries were prepared using the ScriptSeq V2 RNA-seq Kit (Epicentre), again using the low input protocol.

    FACS:

    Article Title: Purification of high-quality RNA from a small number of fluorescence activated cell sorted zebrafish cells for RNA sequencing purposes
    Article Snippet: .. Based on different quality parameters and comparison of various experimental set-ups, we recommend using the RNeasy Plus Micro Kit for RNA isolation of FACS sorted zebrafish cells. .. The RNA purification protocol is fast (only 20–30 min), easy and consistently delivers high-quality RNA samples.

    Similar Products

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  • 99
    Qiagen enscs
    Effect on gene expression of Bmi1 overexpression in <t>eNSCs</t> compared to aNSCs. ( A ) Comparison of transcript levels measured by <t>RNA-seq</t> (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P
    Enscs, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enscs/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    enscs - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Journal: Scientific Reports

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    doi: 10.1038/s41598-018-25921-8

    Figure Lengend Snippet: Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Article Snippet: High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples.

    Techniques: Expressing, Over Expression, RNA Sequencing Assay, Plasmid Preparation

    Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Journal: Scientific Reports

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    doi: 10.1038/s41598-018-25921-8

    Figure Lengend Snippet: Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Article Snippet: High throughput RNA sequencing (RNA-seq) To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples.

    Techniques: Expressing, Over Expression, RNA Sequencing Assay

    Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Journal: Scientific Reports

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    doi: 10.1038/s41598-018-25921-8

    Figure Lengend Snippet: Effect on gene expression of Bmi1 overexpression in eNSCs compared to aNSCs. ( A ) Comparison of transcript levels measured by RNA-seq (reads per kilobase per million reads mapped, RPKM) in embryonic and adult NSCs (empty vector control, average of two biological replicates). ( B ) Gene ontology categories enriched for genes down-regulated (top) or up-regulated (bottom) upon Bmi1 overexpression in eNSCs (red bars) or aNSCs (blue bars). Categories having P

    Article Snippet: To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples.

    Techniques: Expressing, Over Expression, RNA Sequencing Assay, Plasmid Preparation

    Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Journal: Scientific Reports

    Article Title: Effect of Bmi1 over-expression on gene expression in adult and embryonic murine neural stem cells

    doi: 10.1038/s41598-018-25921-8

    Figure Lengend Snippet: Comparison of gene expression changes in embryonic and adult NSCs caused by Bmi1 overexpression. ( A ) Reads per kilobase per million reads (RPKM) from RNA-seq data from aNSCs and eSNCs is plotted for 4296 genes having expression altered at least two-fold by Bmi1 overexpression in either eNSCs or aNSCs. ( B ) Genes plotted in ( A ) were broken down according to their relative expression in eNSCs and aNSCs as indicated.

    Article Snippet: To prepare samples for Illumina sequencing, 150–250 ng of RNA prepared from aNSCs or eNSCs (using the RNAeasy Plus Micro kit; Qiagen) was first treated to remove ribosomal RNA using the Ribo-Zero Magnetic Kit (Epicentre, Madison, WI), using the protocol for low input samples.

    Techniques: Expressing, Over Expression, RNA Sequencing Assay