Journal: Nature neuroscience

Article Title: A glycolytic shift in Schwann cells supports injured axons

doi: 10.1038/s41593-020-0689-4

Figure Lengend Snippet: a-i, Representative immunofluorescence for the indicated metabolic components on longitudinal frozen sections from uninjured control nerve segments and axotomized distal sciatic nerve stumps at the shown post-injury times. HK2: Hexokinase 2 (a). GPI: Glucose-6-phosphate isomerase (b). ALDA: Aldolase A (c). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase (d). PGK1: Phosphoglycerate kinase 1 (e). PGAM1: Phosphoglycerate mutase 1 (f). ENO1: Enolase 1 (g). PKM1: Pyruvate kinase M1 (h). LDHB: Lactate dehydrogenase B (i). Arrows depict colocalization in SCs. Scale bars: 50μm. The experiments for each component were reproduced three times independently with similar results.

Article Snippet: Following primary antibodies were used for nerve and teased fiber immunofluorescence: Neurofilament 200 (1:500, Sigma, N4142), Hexokinase I C35C4 (1:200, Cell Signaling, 2024), Hexokinase II C64G5 (1:200, Cell Signaling, 2867), GPI (1:200, Proteintech, 15171-1-AP), PFKM (1:200, Proteintech, 55028-1-AP), Aldolase A D73H4 (1:200, Cell Signaling, 8060), GAPDH (1:500, Sigma, G9545), PGK1 (1:150, Proteintech, 17811-1-AP), PGAM1 D3J9T (1:50, Cell Signaling, 12098), Enolase-1 D2S1A (1:200, Cell Signaling, 13410), PKM1 D30G6 (1:400, Cell Signaling, 7067), PKM2 D78A4 (1:200, Cell Signaling, 4053), PFKFB3 D7H4Q (1:200, Cell Signaling, 13123), LDHA/LDHC C28H7 (1:200, Cell Signaling, 3558), LDHB (1:200, Proteintech, 14824-1-AP), Glut1 D3J3A (1:200, Cell Signaling, 12939), PDHK1 C47H1 (1:100, Cell Signaling, 3820), Pyruvate Dehydrogenase C54G1 (1:100, Cell Signaling, 3205), CS (1:150, Proteintech, 16131-1-AP), IDH3A (1:100, Proteintech, 15909-1-AP), OGDH (1:50, Proteintech, 15212-1-AP), MCT1 M-45 (1:200, Santa Cruz Biotechnology, sc-50325), MCT4 H-90 (1:250, Santa Cruz Biotechnology, sc-50329), Phospho-S6 Ribosomal Protein (Ser240/244) D68F8 (1:800, Cell Signaling, 5364), Phospho-Akt (Ser473) D9E (1:100, Cell Signaling, 4060), HIF-1 alpha (1:200, Novus Biologicals, NB100-479), c-Myc D84C12 (1:800, Cell Signaling, 5605), c-Myc/N-Myc D3N8F (1:800, Cell Signaling, 13987), Phospho-AMPKα (Thr172) 40H9 (1:100, Cell Signaling, 2535), S100 4C4.9 (1:200, Thermo Fisher Scientific, MA5-12966), P0 (1:1000, Aves Labs, PZO), TUJ1/TUBB3 (1:500, BioLegend, 801202).

Techniques: Expressing, Immunofluorescence