Journal: Alzheimer's Research & Therapy

Article Title: Near-infrared light reduces β-amyloid-stimulated microglial toxicity and enhances survival of neurons: mechanisms of light therapy for Alzheimer’s disease

doi: 10.1186/s13195-022-01022-7

Figure Lengend Snippet: NIR light activation phagocytosis of Aβ-treated microglia cells. Changes in the fluorescence signal from Aβ following stimulation by non-fluorescent Aβ, low-intensity light at 808 nm with 10 J/cm 2 , and both Aβ and light over time was recorded. The representative transmission microscopy images show phagocytosis of Aβ by microglia after 30 min, 2 h, 4 h, 16 h, and 24 h. The enlarged brightfield and merged images are presented in Fig. S , Supplementary Materials. The data are presented as the mean ± SD ( n = 6 replicates in each group); * p < 0.05 indicates data with a statistically significant difference evaluated in relation to the control level, and # p < 0.05 indicates data with a statistically significant difference evaluated in relation to the level during co-cultivation with Aβ-treated microglia (two-way ANOVA test)

Article Snippet: Then, the cells were washed and the fluorescence of non-engulfed Aβ was quenched by addition of 0.2% trypan blue (ThermoFisher Scientific, UK) for 1 min, while the cellular fluorescence was determined using a fluorimeter (Jenway 6270, UK).

Techniques: Activation Assay, Fluorescence, Transmission Assay, Microscopy