endogenous endotoxin core antibody igm  (Hycult Biotech)

Journal: Brain, Behavior, & Immunity - Health

Article Title: Suppressed immune and metabolic responses to intestinal damage-associated microbial translocation in myalgic encephalomyelitis/chronic fatigue syndrome

doi: 10.1016/j.bbih.2023.100627

Figure Lengend Snippet: (a–f) Humoral immune response to microbial antigens. Plasma levels of (a) IgG antibody to LPS (EndoCAb IgG), (b) IgA antibody to LPS (EndoCAb IgA), (c) IgM antibody to LPS (EndoCAb IgM), (d) IgG antibody to flagellin, (e) IgA antibody to flagellin, and (f) IgM antibody to flagellin in cohorts of healthy controls (n = 86) and ME/CFS (n = 131) patients. Data are shown as scatterplots with the red bar indicating the median for each cohort. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Plasma levels of IgG, IgA, and IgM anti-endotoxin (LPS)-core antibodies (EndoCAb) (Hycult Biotech, Uden, The Netherlands), lipopolysaccharide (LPS)-binding protein (LBP) (Hycult Biotech), soluble CD14 (sCD14) (R&D Systems, Minneapolis, MN), and intestinal fatty acid-binding protein (FABP2) (R&D Systems) were determined by ELISA according to the manufacturers’ protocols, as we have previously described ( ).

Techniques:

Journal: Brain, Behavior, & Immunity - Health

Article Title: Suppressed immune and metabolic responses to intestinal damage-associated microbial translocation in myalgic encephalomyelitis/chronic fatigue syndrome

doi: 10.1016/j.bbih.2023.100627

Figure Lengend Snippet: Markers of immune activation and microbial translocation in response to the exercise challenge. (a–f) Levels of LBP (a), sCD14 (b), LPS (c), IgM antibody to LPS (EndoCAb IgM) (d), IgM antibody to gliadin (e) and IL-10 (f) before and after the maximal exercise challenge in unaffected controls (n = 7) and individuals with ME/CFS (n = 9). Each individual is represented by a filled circle and the two points corresponding to the same individual are connected by a line.

Article Snippet: Plasma levels of IgG, IgA, and IgM anti-endotoxin (LPS)-core antibodies (EndoCAb) (Hycult Biotech, Uden, The Netherlands), lipopolysaccharide (LPS)-binding protein (LBP) (Hycult Biotech), soluble CD14 (sCD14) (R&D Systems, Minneapolis, MN), and intestinal fatty acid-binding protein (FABP2) (R&D Systems) were determined by ELISA according to the manufacturers’ protocols, as we have previously described ( ).

Techniques: Activation Assay, Translocation Assay