endonucleases xbai  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    New England Biolabs endonucleases xbai
    Endonucleases Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endonucleases xbai/product/New England Biolabs
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    endonucleases xbai - by Bioz Stars, 2020-03
    92/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: The pCD-GLA plasmid and the minigene cassettes were cut with endonucleases XbaI and EcoRI (New England Biolabs, MA) that each recognize a unique restriction site. .. The digested fragments were linked using Solution I of the DNA Ligation Kit Ver.2.1 (TaKaRa Bio, Kusatsu, Japan) and cloned by transforming the E. coli Solopack gold cells (Agilent Technologies, Santa Clara, CA).

    Amplification:

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: The WT minigene was amplified by PCR with specific mutated oligonucleotide primers GLA919A-fw and GLA919A-rev, in combination with primers 3ECOGLA and 5XBAGLA. .. The pCD-GLA plasmid and the minigene cassette were cut with endonucleases XbaI and EcoRI (New England Biolabs).

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: Two GLA cDNA fragments corresponding to exons 1–4 and exons 5–7 were amplified from pCD-GLA plasmid with primer pairs 5XBAGLA + cDNAcap4R and cDNAcap5F + 3ECOGLA. .. The pCD-GLA plasmid and the minigene cassettes were cut with endonucleases XbaI and EcoRI (New England Biolabs, MA) that each recognize a unique restriction site.

    Inverse PCR:

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: The pCD-GLA plasmid and the minigene cassette were cut with endonucleases XbaI and EcoRI (New England Biolabs). .. We obtained plasmids U1-GLA3T, U1-GLA5A, U1-GLAWT and U1-GLAScramble by inverse PCR of the U1 snRNA genomic sequence, as described previously , using the oligonucleotides (Eurofins MWG Operon company, Ebersberg, Germany) reported in .

    DNA Ligation:

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: The pCD-GLA plasmid and the minigene cassettes were cut with endonucleases XbaI and EcoRI (New England Biolabs, MA) that each recognize a unique restriction site. .. The digested fragments were linked using Solution I of the DNA Ligation Kit Ver.2.1 (TaKaRa Bio, Kusatsu, Japan) and cloned by transforming the E. coli Solopack gold cells (Agilent Technologies, Santa Clara, CA).

    Mutagenesis:

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: To introduce the Asian GLA c.639+919G > A mutation, for which patient-derived genomic DNA was not available, we used general procedures for site directed mutagenesis. .. The pCD-GLA plasmid and the minigene cassette were cut with endonucleases XbaI and EcoRI (New England Biolabs).

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: The entire GLA intron 4 was amplified from human wild-type DNA or from a male patient's DNA harboring the c.639+861C > T GLA mutation. .. The pCD-GLA plasmid and the minigene cassettes were cut with endonucleases XbaI and EcoRI (New England Biolabs, MA) that each recognize a unique restriction site.

    Introduce:

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: To introduce the Asian GLA c.639+919G > A mutation, for which patient-derived genomic DNA was not available, we used general procedures for site directed mutagenesis. .. The pCD-GLA plasmid and the minigene cassette were cut with endonucleases XbaI and EcoRI (New England Biolabs).

    Polymerase Chain Reaction:

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: The obtained PCR fragments were mixed and used as templates for a PCR reaction with external oligonucleotides 5XBAGLA and 3ECOGLA to produce the minigene cassette. .. The pCD-GLA plasmid and the minigene cassette were cut with endonucleases XbaI and EcoRI (New England Biolabs).

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: All the PCR amplifications were performed with high fidelity Platinum Pfx DNA polymerase (Thermo Fisher Scientific, Waltham, MA). .. The pCD-GLA plasmid and the minigene cassettes were cut with endonucleases XbaI and EcoRI (New England Biolabs, MA) that each recognize a unique restriction site.

    Plasmid Preparation:

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: .. The pCD-GLA plasmid and the minigene cassette were cut with endonucleases XbaI and EcoRI (New England Biolabs). ..

    Article Title: Double-target Antisense U1snRNAs Correct Mis-splicing Due to c.639+861C > T and c.639+919G > A GLA Deep Intronic Mutations
    Article Snippet: .. The pCD-GLA plasmid and the minigene cassettes were cut with endonucleases XbaI and EcoRI (New England Biolabs, MA) that each recognize a unique restriction site. .. The digested fragments were linked using Solution I of the DNA Ligation Kit Ver.2.1 (TaKaRa Bio, Kusatsu, Japan) and cloned by transforming the E. coli Solopack gold cells (Agilent Technologies, Santa Clara, CA).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs restriction endonucleases xbai
    (A) <t>PFGE</t> analysis of S . Typhimurium DT104 using <t>XbaI</t> and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,
    Restriction Endonucleases Xbai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases xbai/product/New England Biolabs
    Average 99 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases xbai - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    79
    New England Biolabs xbai endonuclease
    (A) <t>PFGE</t> analysis of S . Typhimurium DT104 using <t>XbaI</t> and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,
    Xbai Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai endonuclease/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xbai endonuclease - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    Image Search Results


    (A) PFGE analysis of S . Typhimurium DT104 using XbaI and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,

    Journal: Applied and Environmental Microbiology

    Article Title: Evidence of Metabolic Switching and Implications for Food Safety from the Phenome(s) of Salmonella enterica Serovar Typhimurium DT104 Cultured at Selected Points across the Pork Production Food Chain

    doi: 10.1128/AEM.01041-13

    Figure Lengend Snippet: (A) PFGE analysis of S . Typhimurium DT104 using XbaI and AvrII. Isolate groups were color identified for simplicity as follows, and the color codes were maintained in all of the assays: reference strain (white); group A, environmental (green); group B,

    Article Snippet: Pulsed-field gel electrophoresis (PFGE) was performed using the restriction endonucleases XbaI and AvrII (New England BioLabs) by following the CDC PulseNet protocol ( ).

    Techniques:

    ( A ) The 482 bp APC gene fragment ( APC cDNA bp 8422–8903) released by digestion of human genomic DNA with endonucleases Hae III and Xba I. The positions of the two biotinylated 30mer probes (BP1 and BP2) for enriching this fragment from genomic DNA are indicated by open bars. The positions of the AT→GC transition at APC cDNA bp 8652 carried by the internal standard and the recognition sites of endonucleases Sau 3AI and Acc I used to liberate the 271 bp APC gene fragment ( APC cDNA bp 8434–8704) are indicated by arrows. ( B ) The melting profile of the 271 bp APC gene fragment was calculated according to the melting algorithm of Lerman and Silverstein (23). The positions of the primers (AP1 and AP4H) used in high fidelity PCR are indicated by filled bars.

    Journal: Nucleic Acids Research

    Article Title: A sensitive scanning technology for low frequency nuclear point mutations in human genomic DNA

    doi:

    Figure Lengend Snippet: ( A ) The 482 bp APC gene fragment ( APC cDNA bp 8422–8903) released by digestion of human genomic DNA with endonucleases Hae III and Xba I. The positions of the two biotinylated 30mer probes (BP1 and BP2) for enriching this fragment from genomic DNA are indicated by open bars. The positions of the AT→GC transition at APC cDNA bp 8652 carried by the internal standard and the recognition sites of endonucleases Sau 3AI and Acc I used to liberate the 271 bp APC gene fragment ( APC cDNA bp 8434–8704) are indicated by arrows. ( B ) The melting profile of the 271 bp APC gene fragment was calculated according to the melting algorithm of Lerman and Silverstein (23). The positions of the primers (AP1 and AP4H) used in high fidelity PCR are indicated by filled bars.

    Article Snippet: The genomic DNA was digested with two ‘inexpensive’ endonucleases, Hae III and Xba I (New England Biolabs, Beverly, MA), at 1 U enzyme/µg DNA and 2–3 mg DNA/ml overnight to liberate the target sequence embedded in a 482 bp fragment representing the APC cDNA bp 8422–8903.

    Techniques: Polymerase Chain Reaction