endonucleases dde  (New England Biolabs)


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    New England Biolabs endonucleases dde
    Endonucleases Dde, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 1 article reviews
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    endonucleases dde - by Bioz Stars, 2020-08
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    Article Snippet: The PCR products obtained from the amplification of the RD2 region of prtF1 were subjected to restriction analysis with endonucleases Dde I, Hae III, and Hin fI (New England Biolabs, Beverly, Mass.).

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    New England Biolabs dde i restriction endonuclease
    Dde I Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs restriction endonucleases dde
    Chromatin remodeling at the Oct-Sox binding motif in Oct4 and Nanog. (A) Schematic showing the location of the Oct-Sox binding motif and adjacent restriction enzyme cut site within the forward and reverse primers for Oct4 and Nanog . Shown are the effects of open and condensed chromatin on the accessibility of <t>Dde</t> I and <t>Mse</t> I at Oct4 and Nanog , respectively. (B) qPCR analysis of restriction enzyme accessibility at the Oct-Sox binding motif in Oct4 and Nanog at 0, 48, and 96 h after induction of Cdx2. As a negative control, the accessibility of Mse I at an intergenic region was assessed. Data are presented as the percentage of DNA cut by the respective restriction enzyme. An assay was performed on a total of three biological replicates. For each biological replicate, two PCR reactions were performed in duplicate. (C) Histone H3 ChIP analysis at the Oct-Sox binding motif in Oct4 and Nanog at 0, 48, and 96 h after induction of Cdx2. The enrichment of histone H3 on Gapdh was utilized as a control. As a negative control, a rabbit nonspecific IgG was used. A total of two ChIP replicates were performed for each biological replicate. * P
    Restriction Endonucleases Dde, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Chromatin remodeling at the Oct-Sox binding motif in Oct4 and Nanog. (A) Schematic showing the location of the Oct-Sox binding motif and adjacent restriction enzyme cut site within the forward and reverse primers for Oct4 and Nanog . Shown are the effects of open and condensed chromatin on the accessibility of Dde I and Mse I at Oct4 and Nanog , respectively. (B) qPCR analysis of restriction enzyme accessibility at the Oct-Sox binding motif in Oct4 and Nanog at 0, 48, and 96 h after induction of Cdx2. As a negative control, the accessibility of Mse I at an intergenic region was assessed. Data are presented as the percentage of DNA cut by the respective restriction enzyme. An assay was performed on a total of three biological replicates. For each biological replicate, two PCR reactions were performed in duplicate. (C) Histone H3 ChIP analysis at the Oct-Sox binding motif in Oct4 and Nanog at 0, 48, and 96 h after induction of Cdx2. The enrichment of histone H3 on Gapdh was utilized as a control. As a negative control, a rabbit nonspecific IgG was used. A total of two ChIP replicates were performed for each biological replicate. * P

    Journal: Stem Cells and Development

    Article Title: Transcriptional Reprogramming and Chromatin Remodeling Accompanies Oct4 and Nanog Silencing in Mouse Trophoblast Lineage

    doi: 10.1089/scd.2013.0328

    Figure Lengend Snippet: Chromatin remodeling at the Oct-Sox binding motif in Oct4 and Nanog. (A) Schematic showing the location of the Oct-Sox binding motif and adjacent restriction enzyme cut site within the forward and reverse primers for Oct4 and Nanog . Shown are the effects of open and condensed chromatin on the accessibility of Dde I and Mse I at Oct4 and Nanog , respectively. (B) qPCR analysis of restriction enzyme accessibility at the Oct-Sox binding motif in Oct4 and Nanog at 0, 48, and 96 h after induction of Cdx2. As a negative control, the accessibility of Mse I at an intergenic region was assessed. Data are presented as the percentage of DNA cut by the respective restriction enzyme. An assay was performed on a total of three biological replicates. For each biological replicate, two PCR reactions were performed in duplicate. (C) Histone H3 ChIP analysis at the Oct-Sox binding motif in Oct4 and Nanog at 0, 48, and 96 h after induction of Cdx2. The enrichment of histone H3 on Gapdh was utilized as a control. As a negative control, a rabbit nonspecific IgG was used. A total of two ChIP replicates were performed for each biological replicate. * P

    Article Snippet: Isolated nuclei were then treated with restriction endonucleases Dde I or Mse I (New England Biolabs) that were specific for single-cut sites located within the amplicons for Oct4 and Nanog , respectively.

    Techniques: Binding Assay, Real-time Polymerase Chain Reaction, Negative Control, Polymerase Chain Reaction, Chromatin Immunoprecipitation