endogenous peroxidase activity  (Abcam)

 
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    Name:
    Myeloperoxidase MPO Activity Assay Kit Colorimetric
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    Catalog Number:
    ab105136
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    Structured Review

    Abcam endogenous peroxidase activity

    https://www.bioz.com/result/endogenous peroxidase activity/product/Abcam
    Average 99 stars, based on 412 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    99/100 stars

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    Blocking Assay:

    Article Title: Histological and Biochemical Comparisons between Right Atrium and Left Atrium in Patients with Mitral Valvular Atrial Fibrillation
    Article Snippet: .. After blocking in TBS-Tween 20 (TBS-T, 0.1% tween 20) containing 5% non-fat dry milk, the membrane was incubated overnight with primary antibodies; pNF-κβ (1:1000 dilution, mouse antihuman monoclonal; Cell Signaling), CD31 (1:1000 dilution, rabbit antihuman polyclonal; Abcam, Cambridge, MA, USA), E-selectin (1:1000 dilution, rabbit antihuman polyclonal; Abcam, Cambridge, MA, USA), iNOS (1:1000 dilution, Abcam, Cambridge, MA, USA), SDF-1α (1:1000 dilution, rabbit antihuman polyclonal; Abcam, Cambridge, MA, USA), ET-1 (1:1000 dilution, mouse antihuman monoclonal; Thermo Scientific, Hudson, NH, USA), PDGF (1:500 dilution, rabbit antihuman polyclonal; Pierce Biotechnology, Rockford, IL, USA), and MPO (1:1000 dilution, rabbit antihuman polyclonal; Abcam, Cambridge, MA, USA). .. The primary antibody incubation was followed by incubation with HRP-conjugated secondary antibodies (Dako, Carpinteria, CA, USA).

    Purification:

    Article Title: Neutrophil microparticles deliver active myeloperoxidase to injured mucosa to inhibit epithelial wound healing
    Article Snippet: .. A function inhibitory anti-CD11b/CD18 mAb (CBRM1/29) was purified in-house as previously described , MPO from Abcam (Cambridge, MA). .. HRP-conjugated anti-mouse and anti-rabbit IgGs from Jackson Immunoresearch (West Grove, PA).

    Incubation:

    Article Title: Histological and Biochemical Comparisons between Right Atrium and Left Atrium in Patients with Mitral Valvular Atrial Fibrillation
    Article Snippet: .. After blocking in TBS-Tween 20 (TBS-T, 0.1% tween 20) containing 5% non-fat dry milk, the membrane was incubated overnight with primary antibodies; pNF-κβ (1:1000 dilution, mouse antihuman monoclonal; Cell Signaling), CD31 (1:1000 dilution, rabbit antihuman polyclonal; Abcam, Cambridge, MA, USA), E-selectin (1:1000 dilution, rabbit antihuman polyclonal; Abcam, Cambridge, MA, USA), iNOS (1:1000 dilution, Abcam, Cambridge, MA, USA), SDF-1α (1:1000 dilution, rabbit antihuman polyclonal; Abcam, Cambridge, MA, USA), ET-1 (1:1000 dilution, mouse antihuman monoclonal; Thermo Scientific, Hudson, NH, USA), PDGF (1:500 dilution, rabbit antihuman polyclonal; Pierce Biotechnology, Rockford, IL, USA), and MPO (1:1000 dilution, rabbit antihuman polyclonal; Abcam, Cambridge, MA, USA). .. The primary antibody incubation was followed by incubation with HRP-conjugated secondary antibodies (Dako, Carpinteria, CA, USA).

    Article Title: Diet-induced vitamin D deficiency has no effect on acute post-stroke outcomes in young male mice
    Article Snippet: .. Next, brain sections were incubated overnight at 4℃ with one of the following primary antibodies: rabbit anti-CD3 (1:200), rabbit anti-myeloperoxidase (1:100; both from Abcam, Cambridge, MA, USA), rat anti-F4/80 (1:100; Biorad; Hercules, CA, USA). .. The following day, sections were washed (PBS; 3 × 10 min) and then incubated for 2 h in either a goat anti-rabbit Alexa Fluor 594 (1:500) or goat anti-rat Alexa Fluor 594 (1:500; both from Thermofisher Scientific, Waltham, MA, USA).

    other:

    Article Title: ADRB3 expression in tumor cells is a poor prognostic factor and promotes proliferation in non-small cell lung carcinoma
    Article Snippet: Antibodies against the following proteins were used: ADRB3, Myeloperoxidase (MPO), IL-6, IFN-γ, CD19, CD68, Nucleolin, which were purchased from Abcam (Cambridge, UK); Ki-67, mTOR, Rictor, p53 and GAPDH, which were purchased from Cell Signaling Technology (Danvers, USA).

    Activity Assay:

    Article Title: Recombinant human proteoglycan-4 reduces phagocytosis of urate crystals and downstream nuclear factor kappa B and inflammasome activation and production of cytokines and chemokines in human and murine macrophages
    Article Snippet: .. Myeloperoxidase (MPO) activity in SF lavage samples was measured using a commercially available kit (Abcam). .. SF lavage and serum urea concentrations of each animal were determined using a urea assay kit (Abcam) and the fold dilution in SF lavage was calculated [ , ].

    Staining:

    Article Title: The interaction between C5a and sphingosine-1-phosphate in neutrophils for antineutrophil cytoplasmic antibody mediated activation
    Article Snippet: .. Next, cells were stained with a saturating dose of mouse monoclonal IgG1 antibody directed against human PR3 (Clone number: WGM2) or MPO (Clone number: 2C7) (Abcam, Cambridge, UK) or with an irrelevant IgG1 control antibody (Biolegend, CA, USA) for 30 minutes. .. Neutrophils were then incubated with phycoerythrin (PE)-conjugated goat anti-mouse antibody (Abcam, Cambridge, UK) in the presence of 0.5 mg/ml heat-aggregated goat IgG.

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    Abcam endogenous peroxidase activity
    Endogenous Peroxidase Activity, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase activity/product/Abcam
    Average 99 stars, based on 412 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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