endogenous 18s rna levels  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Eukaryotic 18S rRNA Endogenous Control VIC TAMRA probe primer limited
    Description:
    The Applied Biosystems Eukaryotic 18S rRNA Endogenous Control VIC ⁄ TAMRA Probe Primer Limited is intended as an endogenous control It allows relative gene expression quantification in cDNA samples when used with other TaqMan gene expression assays Probe is labeled with VIC dye TAMRA dye and the primers are limited Can be used for multiplex or singleplex PCR reactions Endogenous control is to be used with Inventoried and Made to Order TaqMan Gene Expression Assays Custom TaqMan Gene Expression Assays and⁄or Custom TaqMan Primers and Probes Assay Details Gene Symbol 18SRefSeq X03205 1Probe Exon Location NAAmplicon Size 187TaqMan Endogenous ControlsEliminate months of assay design formulation and testing by using TaqMan Endogenous Controls as your controls to quantify gene expression This convenient collection of pre designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction Complete Solution for Quantitative Gene ExpressionHaving a hard time deciding what controls to use to quantify gene expression even with detailed information on biological systems Now with TaqMan Endogenous Controls you can avoid all the trial and error of selecting controls for most common human mouse rat and eukaryotic genes Simple to UseAll components of the TaqMan Endogenous Controls are QC tested formulated into a single 20X mix and functionally tested The controls are not only simple to use but they are also fully compatible with universal conditions for two step RT PCR Just add TaqMan Universal PCR Master Mix with or without AmpErase UNG and your cDNA sample to generate sensitive reproducible and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT 7300 7500 Real Time PCR Systems and the 7000 and 7700 Sequence Detection Systems Flexible OfferingWe build each endogenous control using our proven 5 nuclease chemistry For maximum flexibility you can choose between two different reporter dyes and two quenchers • A FAM dye labeled TaqMan MGB probe 250nM final concentration and two unlabeled PCR primers 900nM each • A VIC dye labeled TaqMan MGB probe 250nM final concentration and two unlabeled PCR primers 150nM each y primer limited • A VIC dye labeled TAMRA probe 250nM final concentration and two unlabeled PCR primers 150nM each y primer limited Choosing the Right Endogenous ControlEndogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells For best results verify that the endogenous control is consistently expressed in the sample set to be tested Endogenous control expression must be uniform across all samples in the study For multiplexing ensure that the gene expression level of the endogenous control is greater than that of the target Multiplex vs Singleplex PCRAll TaqMan Endogenous Controls that contain probes labeled with the VIC reporter dye are primer limited This allows multiplexing of TaqMan Endogenous Controls with target gene expression assays provided that the control gene is more abundantly expressed than the target gene All TaqMan Endogenous Controls that contain probes labeled with the FAM reporter dye are not primer limited and are not intended for multiplexing For Research Use Only Not for use in diagnostic procedures
    Catalog Number:
    4310893e
    Price:
    None
    Applications:
    PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Real-Time PCR Primers, Probes, Arrays & Controls|Gene Expression Analysis & Genotyping
    Category:
    Standards Ladders Controls
    Buy from Supplier


    Structured Review

    Thermo Fisher endogenous 18s rna levels
    The Applied Biosystems Eukaryotic 18S rRNA Endogenous Control VIC ⁄ TAMRA Probe Primer Limited is intended as an endogenous control It allows relative gene expression quantification in cDNA samples when used with other TaqMan gene expression assays Probe is labeled with VIC dye TAMRA dye and the primers are limited Can be used for multiplex or singleplex PCR reactions Endogenous control is to be used with Inventoried and Made to Order TaqMan Gene Expression Assays Custom TaqMan Gene Expression Assays and⁄or Custom TaqMan Primers and Probes Assay Details Gene Symbol 18SRefSeq X03205 1Probe Exon Location NAAmplicon Size 187TaqMan Endogenous ControlsEliminate months of assay design formulation and testing by using TaqMan Endogenous Controls as your controls to quantify gene expression This convenient collection of pre designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction Complete Solution for Quantitative Gene ExpressionHaving a hard time deciding what controls to use to quantify gene expression even with detailed information on biological systems Now with TaqMan Endogenous Controls you can avoid all the trial and error of selecting controls for most common human mouse rat and eukaryotic genes Simple to UseAll components of the TaqMan Endogenous Controls are QC tested formulated into a single 20X mix and functionally tested The controls are not only simple to use but they are also fully compatible with universal conditions for two step RT PCR Just add TaqMan Universal PCR Master Mix with or without AmpErase UNG and your cDNA sample to generate sensitive reproducible and truly quantitative gene expression data on Applied Biosystems instruments including the Applied Biosystems 7900HT 7300 7500 Real Time PCR Systems and the 7000 and 7700 Sequence Detection Systems Flexible OfferingWe build each endogenous control using our proven 5 nuclease chemistry For maximum flexibility you can choose between two different reporter dyes and two quenchers • A FAM dye labeled TaqMan MGB probe 250nM final concentration and two unlabeled PCR primers 900nM each • A VIC dye labeled TaqMan MGB probe 250nM final concentration and two unlabeled PCR primers 150nM each y primer limited • A VIC dye labeled TAMRA probe 250nM final concentration and two unlabeled PCR primers 150nM each y primer limited Choosing the Right Endogenous ControlEndogenous controls can normalize the expression levels of target genes by correcting differences in the amount of cDNA that is loaded into PCR reaction wells For best results verify that the endogenous control is consistently expressed in the sample set to be tested Endogenous control expression must be uniform across all samples in the study For multiplexing ensure that the gene expression level of the endogenous control is greater than that of the target Multiplex vs Singleplex PCRAll TaqMan Endogenous Controls that contain probes labeled with the VIC reporter dye are primer limited This allows multiplexing of TaqMan Endogenous Controls with target gene expression assays provided that the control gene is more abundantly expressed than the target gene All TaqMan Endogenous Controls that contain probes labeled with the FAM reporter dye are not primer limited and are not intended for multiplexing For Research Use Only Not for use in diagnostic procedures
    https://www.bioz.com/result/endogenous 18s rna levels/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    endogenous 18s rna levels - by Bioz Stars, 2021-01
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development
    Article Snippet: .. Relative abundance of target genes to 18S rRNA transcripts was determined by qPCR with SYBR®Green in a GeneAmp 7500 Sequence Detection System (Applied Biosystems). ..

    Amplification:

    Article Title: BACL Is a Novel Brain-Associated, Non-NKC-Encoded Mammalian C-Type Lectin-Like Receptor of the CLEC2 Family
    Article Snippet: .. For normalization, amplification of human TBP or eukaryotic 18S rRNA was monitored using TaqMan® endogenous control assays (Life Technologies). .. In situ Hybridization For in situ hybridization BACL cDNA was cloned into the pBluescript II KS(+) vector and used as template for in vitro transcription with T3 or T7 RNA polymerases and the DIG RNA labeling mix (all from Roche) to generate digoxigenin (DIG)-labeled probes.

    SYBR Green Assay:

    Article Title: Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development
    Article Snippet: .. Relative abundance of target genes to 18S rRNA transcripts was determined by qPCR with SYBR®Green in a GeneAmp 7500 Sequence Detection System (Applied Biosystems). ..

    other:

    Article Title: Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment
    Article Snippet: The transcription level of 18S rRNA was used as an endogenous control.

    Expressing:

    Article Title: Tacrolimus Increases Nox4 Expression in Human Renal Fibroblasts and Induces Fibrosis-Related Genes by Aberrant TGF-Beta Receptor Signalling
    Article Snippet: .. Ribosomal 18S gene expression was used as internal standard (Applied Biosystems, 4310893E). ..

    Article Title: Low Oxygen Tension During Incubation Periods of Chondrocyte Expansion Is Sufficient to Enhance Postexpansion Chondrogenesis
    Article Snippet: .. Expression levels of all genes were normalized to 18S rRNA levels (Eukaryotic 18S rRNA Endogenous Control [FAM MGB Probe]; Applied Biosystems). ..

    Sequencing:

    Article Title: Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development
    Article Snippet: .. Relative abundance of target genes to 18S rRNA transcripts was determined by qPCR with SYBR®Green in a GeneAmp 7500 Sequence Detection System (Applied Biosystems). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher gene exp 18s hs99999901 s1
    Knockdown of components of the DHEA sulfation pathway. A , schematic representation of the DHEA sulfation pathway. Activated sulfate in the form of PAPS is produced by either PAPSS1 or PAPSS2 and then used by the sulfotransferase SULT2A1 to convert DHEA to DHEAS. B and C , siRNA-mediated knockdown of SULT2A1, PAPSS1, or PAPSS2 in adrenocortical NCI-H295R1 cells was verified by real-time PCR and Western blotting. A scrambled oligonucleotide served as control ( ctrl ). Real-time PCR data normalized to <t>18S</t> rRNA, fold-change relative to that control. Densitometric quantification of Western blots revealed knockdown efficiencies of up to 90% on the protein level. Double bands were interpreted as degradation products and jointly analyzed. D , DHEA sulfation was assayed for all knockdowns mentioned above, revealing functional differences between PAPSS1 and PAPSS2 for DHEA sulfation by sulfotransferase SULT2A1. Three biological replicates and their average are shown; each dot consists of at least three technical replicates. Normally distributed data were analyzed by one-way ANOVA ( p value
    Gene Exp 18s Hs99999901 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp 18s hs99999901 s1/product/Thermo Fisher
    Average 99 stars, based on 409 article reviews
    Price from $9.99 to $1999.99
    gene exp 18s hs99999901 s1 - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher 18s ribosomal rna
    ZBTB38 is highly expressed by specific hematopoietic lineages. ( A ) ZBTB38 expression values in select cell subsets with DESeq2 count values over 800 were extracted from ImmGen’s <t>RNA-seq</t> SKYLINE and grouped based on cell type. ( B ) Naïve B cells, antigen-specific (NP + ) dark (DZ, CXCR4 + ) and light (LZ, CD86 + ) zone germinal center B cells (GC, CD19 + GL7 + IgD - ), and splenic plasma cells (SpPC, CD138 + ) were sorted 11 days after immunization of C57BL/6 mice with NP-OVA in alhydrogel, and Zbtb38 RNA levels quantified by quantitative RT- PCR. ZBTB38 expression was first normalized to <t>18S</t> expression level followed by normalization to naïve B cells. Gating strategies are shown in S1 Fig. Mean ± SEM are shown; each symbol represents an individual mouse.
    18s Ribosomal Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 825 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/18s ribosomal rna/product/Thermo Fisher
    Average 92 stars, based on 825 article reviews
    Price from $9.99 to $1999.99
    18s ribosomal rna - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    89
    Thermo Fisher 18s mrna
    Apigenin downregulates CD38 in diabetic rats. ( A ) Representative photographs of immunohistochemical staining of CD38 in the tubulointerstitial area (scale bar: 100 μm). ( B ) Western blotting of CD38 and control β-actin in the renal cortex. ( C ) Densitometric evaluation of the western blotting shown in panel B (n=6). ( D ) Quantitative RT-PCR of CD38 <t>mRNA</t> normalized to <t>18S,</t> in the renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p
    18s Mrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/18s mrna/product/Thermo Fisher
    Average 89 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    18s mrna - by Bioz Stars, 2021-01
    89/100 stars
      Buy from Supplier

    Image Search Results


    Knockdown of components of the DHEA sulfation pathway. A , schematic representation of the DHEA sulfation pathway. Activated sulfate in the form of PAPS is produced by either PAPSS1 or PAPSS2 and then used by the sulfotransferase SULT2A1 to convert DHEA to DHEAS. B and C , siRNA-mediated knockdown of SULT2A1, PAPSS1, or PAPSS2 in adrenocortical NCI-H295R1 cells was verified by real-time PCR and Western blotting. A scrambled oligonucleotide served as control ( ctrl ). Real-time PCR data normalized to 18S rRNA, fold-change relative to that control. Densitometric quantification of Western blots revealed knockdown efficiencies of up to 90% on the protein level. Double bands were interpreted as degradation products and jointly analyzed. D , DHEA sulfation was assayed for all knockdowns mentioned above, revealing functional differences between PAPSS1 and PAPSS2 for DHEA sulfation by sulfotransferase SULT2A1. Three biological replicates and their average are shown; each dot consists of at least three technical replicates. Normally distributed data were analyzed by one-way ANOVA ( p value

    Journal: The Journal of Biological Chemistry

    Article Title: Human DHEA sulfation requires direct interaction between PAPS synthase 2 and DHEA sulfotransferase SULT2A1

    doi: 10.1074/jbc.RA118.002248

    Figure Lengend Snippet: Knockdown of components of the DHEA sulfation pathway. A , schematic representation of the DHEA sulfation pathway. Activated sulfate in the form of PAPS is produced by either PAPSS1 or PAPSS2 and then used by the sulfotransferase SULT2A1 to convert DHEA to DHEAS. B and C , siRNA-mediated knockdown of SULT2A1, PAPSS1, or PAPSS2 in adrenocortical NCI-H295R1 cells was verified by real-time PCR and Western blotting. A scrambled oligonucleotide served as control ( ctrl ). Real-time PCR data normalized to 18S rRNA, fold-change relative to that control. Densitometric quantification of Western blots revealed knockdown efficiencies of up to 90% on the protein level. Double bands were interpreted as degradation products and jointly analyzed. D , DHEA sulfation was assayed for all knockdowns mentioned above, revealing functional differences between PAPSS1 and PAPSS2 for DHEA sulfation by sulfotransferase SULT2A1. Three biological replicates and their average are shown; each dot consists of at least three technical replicates. Normally distributed data were analyzed by one-way ANOVA ( p value

    Article Snippet: Expression levels were normalized to 18S rRNA (HS99999901_s1).

    Techniques: Papanicolaou Stain, Produced, Real-time Polymerase Chain Reaction, Western Blot, Functional Assay

    ZBTB38 is highly expressed by specific hematopoietic lineages. ( A ) ZBTB38 expression values in select cell subsets with DESeq2 count values over 800 were extracted from ImmGen’s RNA-seq SKYLINE and grouped based on cell type. ( B ) Naïve B cells, antigen-specific (NP + ) dark (DZ, CXCR4 + ) and light (LZ, CD86 + ) zone germinal center B cells (GC, CD19 + GL7 + IgD - ), and splenic plasma cells (SpPC, CD138 + ) were sorted 11 days after immunization of C57BL/6 mice with NP-OVA in alhydrogel, and Zbtb38 RNA levels quantified by quantitative RT- PCR. ZBTB38 expression was first normalized to 18S expression level followed by normalization to naïve B cells. Gating strategies are shown in S1 Fig. Mean ± SEM are shown; each symbol represents an individual mouse.

    Journal: bioRxiv

    Article Title: ZBTB38 is dispensable for hematopoiesis and antibody responses

    doi: 10.1101/2020.06.11.145839

    Figure Lengend Snippet: ZBTB38 is highly expressed by specific hematopoietic lineages. ( A ) ZBTB38 expression values in select cell subsets with DESeq2 count values over 800 were extracted from ImmGen’s RNA-seq SKYLINE and grouped based on cell type. ( B ) Naïve B cells, antigen-specific (NP + ) dark (DZ, CXCR4 + ) and light (LZ, CD86 + ) zone germinal center B cells (GC, CD19 + GL7 + IgD - ), and splenic plasma cells (SpPC, CD138 + ) were sorted 11 days after immunization of C57BL/6 mice with NP-OVA in alhydrogel, and Zbtb38 RNA levels quantified by quantitative RT- PCR. ZBTB38 expression was first normalized to 18S expression level followed by normalization to naïve B cells. Gating strategies are shown in S1 Fig. Mean ± SEM are shown; each symbol represents an individual mouse.

    Article Snippet: Zbtb38 transcript levels were normalized to 18S ribosomal RNA, forward 5’-CGGCTACCACATCCAAGGAA-3’ and reverse 5’-GCTGGAATTACCGCGGCT-3’ [ ].

    Techniques: Expressing, RNA Sequencing Assay, Mouse Assay, Quantitative RT-PCR

    Regulation of FOXM1 target genes by SETD1A in metastatic castration-resistant prostate cancer (mCRPC). ( A ) Pie graphs showing numbers of differentially expressed genes in LNCaP and C4-2B cells (left) and genes whose expression was dramatically changed in response to SETD1A knockdown in C4-2B cells (right). ( B ) Heat map showing that most of the total SETD1A-activated genes were overexpressed in C4-2B cells compared to that in LNCaP cells. ( C ) Venn diagram showing overlap of C4-2B specific genes and SETD1A dependent genes in C4-2B cells. ( D ) Heat map for the genes that were overlapping in the Venn diagram in Figure 2 C showing the expression pattern of SETD1A-dependent genes among C4-2B specific genes. ( E ) Gene ontology analysis using SETD1A-activated genes among the C4-2B-activated genes. The length of the bars represents the combined score from the Fisher exact test. Q-values suggest the statistical significance for specific terms. ( F ) Heat map of FOXM1-target gene expression obtained from Enrichr analysis. N, shNS; S, shSETD1A ( G ) Validation of RNA-seq results by RT-qPCR (reverse transcript-quantitate polymerase chain reaction) analysis showing the mRNA level of FOXM1 target genes in C4-2B cells treated with shNS vs. those treated with shSETD1A. The mRNA levels were normalized to that of 18S rRNA. Each value represents mean S.D. * p

    Journal: Cancers

    Article Title: SETD1A Promotes Proliferation of Castration-Resistant Prostate Cancer Cells via FOXM1 Transcription

    doi: 10.3390/cancers12071736

    Figure Lengend Snippet: Regulation of FOXM1 target genes by SETD1A in metastatic castration-resistant prostate cancer (mCRPC). ( A ) Pie graphs showing numbers of differentially expressed genes in LNCaP and C4-2B cells (left) and genes whose expression was dramatically changed in response to SETD1A knockdown in C4-2B cells (right). ( B ) Heat map showing that most of the total SETD1A-activated genes were overexpressed in C4-2B cells compared to that in LNCaP cells. ( C ) Venn diagram showing overlap of C4-2B specific genes and SETD1A dependent genes in C4-2B cells. ( D ) Heat map for the genes that were overlapping in the Venn diagram in Figure 2 C showing the expression pattern of SETD1A-dependent genes among C4-2B specific genes. ( E ) Gene ontology analysis using SETD1A-activated genes among the C4-2B-activated genes. The length of the bars represents the combined score from the Fisher exact test. Q-values suggest the statistical significance for specific terms. ( F ) Heat map of FOXM1-target gene expression obtained from Enrichr analysis. N, shNS; S, shSETD1A ( G ) Validation of RNA-seq results by RT-qPCR (reverse transcript-quantitate polymerase chain reaction) analysis showing the mRNA level of FOXM1 target genes in C4-2B cells treated with shNS vs. those treated with shSETD1A. The mRNA levels were normalized to that of 18S rRNA. Each value represents mean S.D. * p

    Article Snippet: Expression levels were normalized to the expression levels of 18S rRNA.

    Techniques: Expressing, RNA Sequencing Assay, Quantitative RT-PCR, Polymerase Chain Reaction

    SETD1A regulates the transcription of FOXM1 gene. ( A ) The results of RT-qPCR performed using total RNA isolated from LNCaP and C4-2B cells showing the relative mRNA levels of SETD1A and FOXM1 in C4-2B cells compared with those in LNCaP cells. The mRNA levels were normalized to that of 18S rRNA. Each value represents mean ± S.D. * p

    Journal: Cancers

    Article Title: SETD1A Promotes Proliferation of Castration-Resistant Prostate Cancer Cells via FOXM1 Transcription

    doi: 10.3390/cancers12071736

    Figure Lengend Snippet: SETD1A regulates the transcription of FOXM1 gene. ( A ) The results of RT-qPCR performed using total RNA isolated from LNCaP and C4-2B cells showing the relative mRNA levels of SETD1A and FOXM1 in C4-2B cells compared with those in LNCaP cells. The mRNA levels were normalized to that of 18S rRNA. Each value represents mean ± S.D. * p

    Article Snippet: Expression levels were normalized to the expression levels of 18S rRNA.

    Techniques: Quantitative RT-PCR, Isolation

    Apigenin downregulates CD38 in diabetic rats. ( A ) Representative photographs of immunohistochemical staining of CD38 in the tubulointerstitial area (scale bar: 100 μm). ( B ) Western blotting of CD38 and control β-actin in the renal cortex. ( C ) Densitometric evaluation of the western blotting shown in panel B (n=6). ( D ) Quantitative RT-PCR of CD38 mRNA normalized to 18S, in the renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p

    Journal: Aging (Albany NY)

    Article Title: CD38 inhibition by apigenin ameliorates mitochondrial oxidative stress through restoration of the intracellular NAD+/NADH ratio and Sirt3 activity in renal tubular cells in diabetic rats

    doi: 10.18632/aging.103410

    Figure Lengend Snippet: Apigenin downregulates CD38 in diabetic rats. ( A ) Representative photographs of immunohistochemical staining of CD38 in the tubulointerstitial area (scale bar: 100 μm). ( B ) Western blotting of CD38 and control β-actin in the renal cortex. ( C ) Densitometric evaluation of the western blotting shown in panel B (n=6). ( D ) Quantitative RT-PCR of CD38 mRNA normalized to 18S, in the renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p

    Article Snippet: The data were normalized to the level of 18S mRNA, which was used as an internal control, as previously described [ ].

    Techniques: Immunohistochemistry, Staining, Western Blot, Quantitative RT-PCR, Standard Deviation

    Apigenin ameliorates renal fibrosis and inflammatory gene expression in diabetic rats. ( A ) Representative photographs of MT staining (scale bar: 100 μm) and IHC of Kim-1 in the tubulointerstitial area (scale bar: 100 μm) and the mitochondrial morphology observed under transmission electron microscopy (TM) scale bar: 500 nm). ( B – E ) Quantitative RT-PCR of collagen III ( B ), Kim-1 ( C ), TNF-α ( D ), and IL-6 ( E ) mRNA normalized to expression of 18S, in the renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p

    Journal: Aging (Albany NY)

    Article Title: CD38 inhibition by apigenin ameliorates mitochondrial oxidative stress through restoration of the intracellular NAD+/NADH ratio and Sirt3 activity in renal tubular cells in diabetic rats

    doi: 10.18632/aging.103410

    Figure Lengend Snippet: Apigenin ameliorates renal fibrosis and inflammatory gene expression in diabetic rats. ( A ) Representative photographs of MT staining (scale bar: 100 μm) and IHC of Kim-1 in the tubulointerstitial area (scale bar: 100 μm) and the mitochondrial morphology observed under transmission electron microscopy (TM) scale bar: 500 nm). ( B – E ) Quantitative RT-PCR of collagen III ( B ), Kim-1 ( C ), TNF-α ( D ), and IL-6 ( E ) mRNA normalized to expression of 18S, in the renal cortex (n=6). All data represent the mean ± standard deviation (SD). *p

    Article Snippet: The data were normalized to the level of 18S mRNA, which was used as an internal control, as previously described [ ].

    Techniques: Expressing, Staining, Immunohistochemistry, Transmission Assay, Electron Microscopy, Quantitative RT-PCR, Standard Deviation