end repair module  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    NEBNext End Repair Module
    Description:
    NEBNext End Repair Module 100 rxns
    Catalog Number:
    e6050l
    Price:
    354
    Size:
    100 rxns
    Category:
    DNA Template Preparation for PCR
    Buy from Supplier


    Structured Review

    New England Biolabs end repair module
    NEBNext End Repair Module
    NEBNext End Repair Module 100 rxns
    https://www.bioz.com/result/end repair module/product/New England Biolabs
    Average 99 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    end repair module - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

    Amplification:

    Article Title: Multiplexed Nanopore Sequencing of HLA-B Locus in Māori and Pacific Island Samples
    Article Snippet: .. Briefly, 5 μg of purified amplicon library was prepared with the NEBNext end repair module (New England Biolabs), then dA-tailed using the NEBNext dA-tailing module (New England Biolabs). .. The final prepared library from 49 participants was loaded into the MinION R9.4 flowcell (Oxford Nanopore Technologies).

    Article Title: Architectural proteins and pluripotency factors cooperate to orchestrate the transcriptional response of hESCs to temperature stress
    Article Snippet: .. Fragment ends were repaired using the NEBNext End Repair Module and adenosine was added at the 3’ ends of fragments using Klenow fragment (3’ to 5’ exo minus, New England Biolabs), universal adaptors were ligated to the A-tailed DNA fragments at room temperature for 1 h with T4 DNA ligase (New England Biolabs) and amplified with Illumina barcoded primers using KAPA SYBR FAST qPCR Master Mix for 5~12 PCR cycles to obtain enough DNA for sequencing. .. Generated libraries were paired-end sequenced on Illumina HiSeq2500 v4.

    Article Title: RNA structure replaces the need for U2AF2 in splicing
    Article Snippet: .. To build a sequenceable library, the resuspended RNA was (1) reverse-transcribed into cDNA by SuperScript II (Invitrogen) and random 9-mers, (2) made into double-stranded cDNA with the NEBNext mRNA Second Strand Synthesis Module (NEB), (3) made into blunt-end dsDNA with the NEBNext End Repair Module (NEB), (4) made into dsDNA with a single A overhang with NEBNext dA-Tailing Module (NEB), (5) ligated with Illumina sequencing adaptors with NEBNext Quick Ligation Module (NEB), and (6) amplified to meet the sequencing requirement by NEBNext High-Fidelity 2× PCR Master Mix (NEB) and two outer primers recognizing the ligated Illumina adaptors (5′-AATGATACGGCGACCACCGAGATCTACAC and 5′-CAAGCAGAAGACGGCATACGAGAT). ..

    Ligation:

    Article Title: A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies.
    Article Snippet: .. Double-stranded DNA was subjected to end repair and dA-tailing using the NEBNext End Repair and dA-TailingModules (NEB E6050L and E6053L) followed by ligation of barcode adapters (IDT) with Cell 181, 1–15.e1–e9, April 16, 2020 e4 Please cite this article in press as: McWhite et al., A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies, Cell (2020), https://doi.org/10.1016/j.cell.2020.02.049 T4 ligase (NEB). .. Following PCR amplification, the quality of the final library was confirmed by Bioanalyzer (Agilent).

    Article Title: RNA structure replaces the need for U2AF2 in splicing
    Article Snippet: .. To build a sequenceable library, the resuspended RNA was (1) reverse-transcribed into cDNA by SuperScript II (Invitrogen) and random 9-mers, (2) made into double-stranded cDNA with the NEBNext mRNA Second Strand Synthesis Module (NEB), (3) made into blunt-end dsDNA with the NEBNext End Repair Module (NEB), (4) made into dsDNA with a single A overhang with NEBNext dA-Tailing Module (NEB), (5) ligated with Illumina sequencing adaptors with NEBNext Quick Ligation Module (NEB), and (6) amplified to meet the sequencing requirement by NEBNext High-Fidelity 2× PCR Master Mix (NEB) and two outer primers recognizing the ligated Illumina adaptors (5′-AATGATACGGCGACCACCGAGATCTACAC and 5′-CAAGCAGAAGACGGCATACGAGAT). ..

    Construct:

    Article Title: The genetics of an early Neolithic pastoralist from the Zagros, Iran
    Article Snippet: .. Indexed Illumina sequencing libraries were constructed with a protocol based on Meyer et al . with modifications including blunt end repair using NEBNext End Repair Module (New England BioLabs Inc) and heat inactivation of Bst DNA polymerase . .. Sequence processing and alignment Libraries were sequenced over a flow cell on a HiSeq 2000 at the TheragenEtex (South Korea) using 100 bp single-end sequencing.

    Purification:

    Article Title: Multiplexed Nanopore Sequencing of HLA-B Locus in Māori and Pacific Island Samples
    Article Snippet: .. Briefly, 5 μg of purified amplicon library was prepared with the NEBNext end repair module (New England Biolabs), then dA-tailed using the NEBNext dA-tailing module (New England Biolabs). .. The final prepared library from 49 participants was loaded into the MinION R9.4 flowcell (Oxford Nanopore Technologies).

    Real-time Polymerase Chain Reaction:

    Article Title: Architectural proteins and pluripotency factors cooperate to orchestrate the transcriptional response of hESCs to temperature stress
    Article Snippet: .. Fragment ends were repaired using the NEBNext End Repair Module and adenosine was added at the 3’ ends of fragments using Klenow fragment (3’ to 5’ exo minus, New England Biolabs), universal adaptors were ligated to the A-tailed DNA fragments at room temperature for 1 h with T4 DNA ligase (New England Biolabs) and amplified with Illumina barcoded primers using KAPA SYBR FAST qPCR Master Mix for 5~12 PCR cycles to obtain enough DNA for sequencing. .. Generated libraries were paired-end sequenced on Illumina HiSeq2500 v4.

    Sequencing:

    Article Title: The genetics of an early Neolithic pastoralist from the Zagros, Iran
    Article Snippet: .. Indexed Illumina sequencing libraries were constructed with a protocol based on Meyer et al . with modifications including blunt end repair using NEBNext End Repair Module (New England BioLabs Inc) and heat inactivation of Bst DNA polymerase . .. Sequence processing and alignment Libraries were sequenced over a flow cell on a HiSeq 2000 at the TheragenEtex (South Korea) using 100 bp single-end sequencing.

    Article Title: Architectural proteins and pluripotency factors cooperate to orchestrate the transcriptional response of hESCs to temperature stress
    Article Snippet: .. Fragment ends were repaired using the NEBNext End Repair Module and adenosine was added at the 3’ ends of fragments using Klenow fragment (3’ to 5’ exo minus, New England Biolabs), universal adaptors were ligated to the A-tailed DNA fragments at room temperature for 1 h with T4 DNA ligase (New England Biolabs) and amplified with Illumina barcoded primers using KAPA SYBR FAST qPCR Master Mix for 5~12 PCR cycles to obtain enough DNA for sequencing. .. Generated libraries were paired-end sequenced on Illumina HiSeq2500 v4.

    Article Title: RNA structure replaces the need for U2AF2 in splicing
    Article Snippet: .. To build a sequenceable library, the resuspended RNA was (1) reverse-transcribed into cDNA by SuperScript II (Invitrogen) and random 9-mers, (2) made into double-stranded cDNA with the NEBNext mRNA Second Strand Synthesis Module (NEB), (3) made into blunt-end dsDNA with the NEBNext End Repair Module (NEB), (4) made into dsDNA with a single A overhang with NEBNext dA-Tailing Module (NEB), (5) ligated with Illumina sequencing adaptors with NEBNext Quick Ligation Module (NEB), and (6) amplified to meet the sequencing requirement by NEBNext High-Fidelity 2× PCR Master Mix (NEB) and two outer primers recognizing the ligated Illumina adaptors (5′-AATGATACGGCGACCACCGAGATCTACAC and 5′-CAAGCAGAAGACGGCATACGAGAT). ..

    Polymerase Chain Reaction:

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

    Article Title: Architectural proteins and pluripotency factors cooperate to orchestrate the transcriptional response of hESCs to temperature stress
    Article Snippet: .. Fragment ends were repaired using the NEBNext End Repair Module and adenosine was added at the 3’ ends of fragments using Klenow fragment (3’ to 5’ exo minus, New England Biolabs), universal adaptors were ligated to the A-tailed DNA fragments at room temperature for 1 h with T4 DNA ligase (New England Biolabs) and amplified with Illumina barcoded primers using KAPA SYBR FAST qPCR Master Mix for 5~12 PCR cycles to obtain enough DNA for sequencing. .. Generated libraries were paired-end sequenced on Illumina HiSeq2500 v4.

    Article Title: RNA structure replaces the need for U2AF2 in splicing
    Article Snippet: .. To build a sequenceable library, the resuspended RNA was (1) reverse-transcribed into cDNA by SuperScript II (Invitrogen) and random 9-mers, (2) made into double-stranded cDNA with the NEBNext mRNA Second Strand Synthesis Module (NEB), (3) made into blunt-end dsDNA with the NEBNext End Repair Module (NEB), (4) made into dsDNA with a single A overhang with NEBNext dA-Tailing Module (NEB), (5) ligated with Illumina sequencing adaptors with NEBNext Quick Ligation Module (NEB), and (6) amplified to meet the sequencing requirement by NEBNext High-Fidelity 2× PCR Master Mix (NEB) and two outer primers recognizing the ligated Illumina adaptors (5′-AATGATACGGCGACCACCGAGATCTACAC and 5′-CAAGCAGAAGACGGCATACGAGAT). ..

    Nanopore Sequencing:

    Article Title: Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples
    Article Snippet: .. Briefly, the protocol for preparing a nanopore sequencing with SQK-MAP004 is as follows: 500 ng to 1μg of PCR products or cloned HBV DNA were diluted to 80μL using nuclease-free water and end-repaired using the NEBNext End Repair Module (New England Biolabs, Evry, France). .. The end-repaired products were then purified using 1.0 x volume of AMPure XP beads (Beckman-Coulter) and eluted in 25μL of nuclease-free.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs nebnext end repair module
    Genes and repetitive DNA in centromeres. (A) Overlap between CENH3 enrichment and genetic elements. Within the centromere, we assigned each 20-kb locus into one of two categories, “low CENH3 loci” and “high CENH3 loci,” based on whether it exhibited less than or greater than the median CENH3 enrichment. Results are shown for three methods of measuring CENH3 enrichment. The first two are relative to a total nucleosome control produced by MNase digestion of total chromatin and relative to a randomly sheared naked DNA control produced by <t>NEBNext</t> dsDNA Fragmentase ( Gent et al. 2014 ). In both cases, only uniquely mapping reads were considered. In the third method, no control was used, and enrichment was defined by raw read counts, including nonuniquely mapping reads. Errors bars are standard errors of the means for each set of loci. (B) Comparison of genetic elements in centromeric 20-kb loci and whole-genome 20-kb loci, but without regard to relative CENH3 enrichment within centromeres
    Nebnext End Repair Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext end repair module/product/New England Biolabs
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    nebnext end repair module - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    93
    New England Biolabs nebnext ultra ii end repair da tailing module
    Genes and repetitive DNA in centromeres. (A) Overlap between CENH3 enrichment and genetic elements. Within the centromere, we assigned each 20-kb locus into one of two categories, “low CENH3 loci” and “high CENH3 loci,” based on whether it exhibited less than or greater than the median CENH3 enrichment. Results are shown for three methods of measuring CENH3 enrichment. The first two are relative to a total nucleosome control produced by MNase digestion of total chromatin and relative to a randomly sheared naked DNA control produced by <t>NEBNext</t> dsDNA Fragmentase ( Gent et al. 2014 ). In both cases, only uniquely mapping reads were considered. In the third method, no control was used, and enrichment was defined by raw read counts, including nonuniquely mapping reads. Errors bars are standard errors of the means for each set of loci. (B) Comparison of genetic elements in centromeric 20-kb loci and whole-genome 20-kb loci, but without regard to relative CENH3 enrichment within centromeres
    Nebnext Ultra Ii End Repair Da Tailing Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii end repair da tailing module/product/New England Biolabs
    Average 93 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii end repair da tailing module - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Genes and repetitive DNA in centromeres. (A) Overlap between CENH3 enrichment and genetic elements. Within the centromere, we assigned each 20-kb locus into one of two categories, “low CENH3 loci” and “high CENH3 loci,” based on whether it exhibited less than or greater than the median CENH3 enrichment. Results are shown for three methods of measuring CENH3 enrichment. The first two are relative to a total nucleosome control produced by MNase digestion of total chromatin and relative to a randomly sheared naked DNA control produced by NEBNext dsDNA Fragmentase ( Gent et al. 2014 ). In both cases, only uniquely mapping reads were considered. In the third method, no control was used, and enrichment was defined by raw read counts, including nonuniquely mapping reads. Errors bars are standard errors of the means for each set of loci. (B) Comparison of genetic elements in centromeric 20-kb loci and whole-genome 20-kb loci, but without regard to relative CENH3 enrichment within centromeres

    Journal: Genetics

    Article Title: Stable Patterns of CENH3 Occupancy Through Maize Lineages Containing Genetically Similar Centromeres

    doi: 10.1534/genetics.115.177360

    Figure Lengend Snippet: Genes and repetitive DNA in centromeres. (A) Overlap between CENH3 enrichment and genetic elements. Within the centromere, we assigned each 20-kb locus into one of two categories, “low CENH3 loci” and “high CENH3 loci,” based on whether it exhibited less than or greater than the median CENH3 enrichment. Results are shown for three methods of measuring CENH3 enrichment. The first two are relative to a total nucleosome control produced by MNase digestion of total chromatin and relative to a randomly sheared naked DNA control produced by NEBNext dsDNA Fragmentase ( Gent et al. 2014 ). In both cases, only uniquely mapping reads were considered. In the third method, no control was used, and enrichment was defined by raw read counts, including nonuniquely mapping reads. Errors bars are standard errors of the means for each set of loci. (B) Comparison of genetic elements in centromeric 20-kb loci and whole-genome 20-kb loci, but without regard to relative CENH3 enrichment within centromeres

    Article Snippet: We end-repaired and 5′-phosphorylated using the End Repair Module (New England Biolabs #E6050L), A-tailed with Klenow exo- (New England Biolabs #M0212S), and ligated to adapters (8.3 nM) with a Quick Ligase Kit (New England Biolabs #M2200S).

    Techniques: Produced