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Azenta
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Creative Biolabs
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Lucigen Corp
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Philips Healthcare
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Cell Biolabs Inc
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Angelman Syndrome Foundation
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Galectin Therapeutics
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Twist Bioscience
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Journal: Synthetic and Systems Biotechnology
Article Title: Metabolic engineering and adaptive laboratory evolution enhance squalene production in Yarrowia lipolytica
doi: 10.1016/j.synbio.2026.01.017
Figure Lengend Snippet: Schematic of constructing engineered Y. lipolytica strain for squalene production. I indicate lipid droplet engineering to modulate triacylglycerols synthesis and increase squalene storage capacity. II indicates modulation of the MVA pathway to promote squalene synthesis flux. III indicates enzyme fusion engineering to promote catalytic efficiency between ERG20 and SQS. IV indicates iterative copy numbering of the ScHMG1 gene. V indicates adaptive evolutionary engineering strategies. Red font represents endogenous genes, and blue font represents heterologous genes. DGA1, Diacylglycerol acyltransferase; LRO1, Phospholipid: diacylglycerol acyltransferase; ERG10, Acetyl-CoA acetyltransferase; ERG13, HMG-CoA synthase; ScHMG1, 3-hydroxy-3-methyl glutaryl coenzyme A reductase of S. cerevisiae origin; ERG20, farnesyl diphosphate synthase; SQS, squalene synthase.
Article Snippet: The
Techniques:
Journal: Synthetic and Systems Biotechnology
Article Title: Metabolic engineering and adaptive laboratory evolution enhance squalene production in Yarrowia lipolytica
doi: 10.1016/j.synbio.2026.01.017
Figure Lengend Snippet: Enhancing the expression of ScHMG1 to boost squalene synthesis . (A) Schematic design of overexpressing ScHMG1 at the IntC locus. (B) Effects of enhanced ScHMG1 expression on squalene and lipid synthesis in the engineered Y. lipolytica strain. (C) The pH, biomass, and glucose consumption of the engineered strains after 72 h of fermentation.
Article Snippet: The
Techniques: Expressing