rnase a  (Jena Bioscience)


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    Name:
    RNase A
    Description:

    Catalog Number:
    EN-179L
    Price:
    374.6
    Category:
    Molecular Biology
    Size:
    5 x 100 mg
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    Structured Review

    Jena Bioscience rnase a
    Protein-responsive protein release from the composite hydrogel. a Schematic illustration of the protocol of protein-responsive protein release from the composite hydrogel. PDB ID: 5ZZE (myoglobin, Mb) and 1AFK (RNase A). b The Mb entrapment ratio of agarose and composite hydrogels ( n = 3). APmoc-F(CF 3 )F hydrogel could not be taken out of the mold. c High-resolution Airyscan CLSM images of the composite hydrogel containing Ax647-Mb (Ax647-Mb⊂ APmoc-F(CF 3 )F /FL-agarose/ TMR-Gua ). r : Pearson’s correlation coefficient. As shown in Supplementary Fig. 31 , Ax647-Mb was trapped more efficiently in the composite hydrogel than the agarose hydrogel. The fibrous morphology of the Alexa Fluor 647 dye could not been observed, implying that interaction between APmoc-F(CF 3 )F fibers and Mb is crucial (Supplementary Fig. 32 ). Please see Supplementary Fig. 33 for other control conditions. d Avidin-responsive Mb release and ( e ) anti-DNP IgG-responsive <t>RNase</t> A release from the composite hydrogel ( n = 3). Condition: [ APmoc-F(CF 3 )F ] = 0.6 wt%, [agarose] = 0.5 wt% (for b , d and e ), [FL-agarose] = 0.5 wt% (for c ), [Mb] = 36 µM (for b and d ), [Ax647-Mb] = 36 µM (for c ), [RNase A] = 0.25 mg/mL (for e ), [ TMR-Gua ] = 10 µM (for c ), [bCAII] = 10 µM, [ EAT(avidin) ] = 20 µM (for d ), [avidin] = 20 µM (for d ), [ EAT(DNP IgG) ] = 15 µM (for e ), [anti-DNP IgG] = 15 µM (for e ), 100 mM HEPES, pH 8.0. The data represent the mean ± standard deviation.

    https://www.bioz.com/result/rnase a/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase a - by Bioz Stars, 2021-06
    93/100 stars

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    1) Product Images from "Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems"

    Article Title: Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems

    Journal: Nature Communications

    doi: 10.1038/s41467-020-17698-0

    Protein-responsive protein release from the composite hydrogel. a Schematic illustration of the protocol of protein-responsive protein release from the composite hydrogel. PDB ID: 5ZZE (myoglobin, Mb) and 1AFK (RNase A). b The Mb entrapment ratio of agarose and composite hydrogels ( n = 3). APmoc-F(CF 3 )F hydrogel could not be taken out of the mold. c High-resolution Airyscan CLSM images of the composite hydrogel containing Ax647-Mb (Ax647-Mb⊂ APmoc-F(CF 3 )F /FL-agarose/ TMR-Gua ). r : Pearson’s correlation coefficient. As shown in Supplementary Fig. 31 , Ax647-Mb was trapped more efficiently in the composite hydrogel than the agarose hydrogel. The fibrous morphology of the Alexa Fluor 647 dye could not been observed, implying that interaction between APmoc-F(CF 3 )F fibers and Mb is crucial (Supplementary Fig. 32 ). Please see Supplementary Fig. 33 for other control conditions. d Avidin-responsive Mb release and ( e ) anti-DNP IgG-responsive RNase A release from the composite hydrogel ( n = 3). Condition: [ APmoc-F(CF 3 )F ] = 0.6 wt%, [agarose] = 0.5 wt% (for b , d and e ), [FL-agarose] = 0.5 wt% (for c ), [Mb] = 36 µM (for b and d ), [Ax647-Mb] = 36 µM (for c ), [RNase A] = 0.25 mg/mL (for e ), [ TMR-Gua ] = 10 µM (for c ), [bCAII] = 10 µM, [ EAT(avidin) ] = 20 µM (for d ), [avidin] = 20 µM (for d ), [ EAT(DNP IgG) ] = 15 µM (for e ), [anti-DNP IgG] = 15 µM (for e ), 100 mM HEPES, pH 8.0. The data represent the mean ± standard deviation.
    Figure Legend Snippet: Protein-responsive protein release from the composite hydrogel. a Schematic illustration of the protocol of protein-responsive protein release from the composite hydrogel. PDB ID: 5ZZE (myoglobin, Mb) and 1AFK (RNase A). b The Mb entrapment ratio of agarose and composite hydrogels ( n = 3). APmoc-F(CF 3 )F hydrogel could not be taken out of the mold. c High-resolution Airyscan CLSM images of the composite hydrogel containing Ax647-Mb (Ax647-Mb⊂ APmoc-F(CF 3 )F /FL-agarose/ TMR-Gua ). r : Pearson’s correlation coefficient. As shown in Supplementary Fig. 31 , Ax647-Mb was trapped more efficiently in the composite hydrogel than the agarose hydrogel. The fibrous morphology of the Alexa Fluor 647 dye could not been observed, implying that interaction between APmoc-F(CF 3 )F fibers and Mb is crucial (Supplementary Fig. 32 ). Please see Supplementary Fig. 33 for other control conditions. d Avidin-responsive Mb release and ( e ) anti-DNP IgG-responsive RNase A release from the composite hydrogel ( n = 3). Condition: [ APmoc-F(CF 3 )F ] = 0.6 wt%, [agarose] = 0.5 wt% (for b , d and e ), [FL-agarose] = 0.5 wt% (for c ), [Mb] = 36 µM (for b and d ), [Ax647-Mb] = 36 µM (for c ), [RNase A] = 0.25 mg/mL (for e ), [ TMR-Gua ] = 10 µM (for c ), [bCAII] = 10 µM, [ EAT(avidin) ] = 20 µM (for d ), [avidin] = 20 µM (for d ), [ EAT(DNP IgG) ] = 15 µM (for e ), [anti-DNP IgG] = 15 µM (for e ), 100 mM HEPES, pH 8.0. The data represent the mean ± standard deviation.

    Techniques Used: Confocal Laser Scanning Microscopy, Avidin-Biotin Assay, Standard Deviation

    Related Articles

    Activity Assay:

    Article Title: Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems
    Article Snippet: .. Determination of the activity of RNase AThe enzymatic activity of RNase A was monitored by DNase+RNase detection kit (Jena Bioscience). ..

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    Jena Bioscience rnase a
    Protein-responsive protein release from the composite hydrogel. a Schematic illustration of the protocol of protein-responsive protein release from the composite hydrogel. PDB ID: 5ZZE (myoglobin, Mb) and 1AFK (RNase A). b The Mb entrapment ratio of agarose and composite hydrogels ( n = 3). APmoc-F(CF 3 )F hydrogel could not be taken out of the mold. c High-resolution Airyscan CLSM images of the composite hydrogel containing Ax647-Mb (Ax647-Mb⊂ APmoc-F(CF 3 )F /FL-agarose/ TMR-Gua ). r : Pearson’s correlation coefficient. As shown in Supplementary Fig. 31 , Ax647-Mb was trapped more efficiently in the composite hydrogel than the agarose hydrogel. The fibrous morphology of the Alexa Fluor 647 dye could not been observed, implying that interaction between APmoc-F(CF 3 )F fibers and Mb is crucial (Supplementary Fig. 32 ). Please see Supplementary Fig. 33 for other control conditions. d Avidin-responsive Mb release and ( e ) anti-DNP IgG-responsive <t>RNase</t> A release from the composite hydrogel ( n = 3). Condition: [ APmoc-F(CF 3 )F ] = 0.6 wt%, [agarose] = 0.5 wt% (for b , d and e ), [FL-agarose] = 0.5 wt% (for c ), [Mb] = 36 µM (for b and d ), [Ax647-Mb] = 36 µM (for c ), [RNase A] = 0.25 mg/mL (for e ), [ TMR-Gua ] = 10 µM (for c ), [bCAII] = 10 µM, [ EAT(avidin) ] = 20 µM (for d ), [avidin] = 20 µM (for d ), [ EAT(DNP IgG) ] = 15 µM (for e ), [anti-DNP IgG] = 15 µM (for e ), 100 mM HEPES, pH 8.0. The data represent the mean ± standard deviation.
    Rnase A, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase a/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase a - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

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    Protein-responsive protein release from the composite hydrogel. a Schematic illustration of the protocol of protein-responsive protein release from the composite hydrogel. PDB ID: 5ZZE (myoglobin, Mb) and 1AFK (RNase A). b The Mb entrapment ratio of agarose and composite hydrogels ( n = 3). APmoc-F(CF 3 )F hydrogel could not be taken out of the mold. c High-resolution Airyscan CLSM images of the composite hydrogel containing Ax647-Mb (Ax647-Mb⊂ APmoc-F(CF 3 )F /FL-agarose/ TMR-Gua ). r : Pearson’s correlation coefficient. As shown in Supplementary Fig. 31 , Ax647-Mb was trapped more efficiently in the composite hydrogel than the agarose hydrogel. The fibrous morphology of the Alexa Fluor 647 dye could not been observed, implying that interaction between APmoc-F(CF 3 )F fibers and Mb is crucial (Supplementary Fig. 32 ). Please see Supplementary Fig. 33 for other control conditions. d Avidin-responsive Mb release and ( e ) anti-DNP IgG-responsive RNase A release from the composite hydrogel ( n = 3). Condition: [ APmoc-F(CF 3 )F ] = 0.6 wt%, [agarose] = 0.5 wt% (for b , d and e ), [FL-agarose] = 0.5 wt% (for c ), [Mb] = 36 µM (for b and d ), [Ax647-Mb] = 36 µM (for c ), [RNase A] = 0.25 mg/mL (for e ), [ TMR-Gua ] = 10 µM (for c ), [bCAII] = 10 µM, [ EAT(avidin) ] = 20 µM (for d ), [avidin] = 20 µM (for d ), [ EAT(DNP IgG) ] = 15 µM (for e ), [anti-DNP IgG] = 15 µM (for e ), 100 mM HEPES, pH 8.0. The data represent the mean ± standard deviation.

    Journal: Nature Communications

    Article Title: Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems

    doi: 10.1038/s41467-020-17698-0

    Figure Lengend Snippet: Protein-responsive protein release from the composite hydrogel. a Schematic illustration of the protocol of protein-responsive protein release from the composite hydrogel. PDB ID: 5ZZE (myoglobin, Mb) and 1AFK (RNase A). b The Mb entrapment ratio of agarose and composite hydrogels ( n = 3). APmoc-F(CF 3 )F hydrogel could not be taken out of the mold. c High-resolution Airyscan CLSM images of the composite hydrogel containing Ax647-Mb (Ax647-Mb⊂ APmoc-F(CF 3 )F /FL-agarose/ TMR-Gua ). r : Pearson’s correlation coefficient. As shown in Supplementary Fig. 31 , Ax647-Mb was trapped more efficiently in the composite hydrogel than the agarose hydrogel. The fibrous morphology of the Alexa Fluor 647 dye could not been observed, implying that interaction between APmoc-F(CF 3 )F fibers and Mb is crucial (Supplementary Fig. 32 ). Please see Supplementary Fig. 33 for other control conditions. d Avidin-responsive Mb release and ( e ) anti-DNP IgG-responsive RNase A release from the composite hydrogel ( n = 3). Condition: [ APmoc-F(CF 3 )F ] = 0.6 wt%, [agarose] = 0.5 wt% (for b , d and e ), [FL-agarose] = 0.5 wt% (for c ), [Mb] = 36 µM (for b and d ), [Ax647-Mb] = 36 µM (for c ), [RNase A] = 0.25 mg/mL (for e ), [ TMR-Gua ] = 10 µM (for c ), [bCAII] = 10 µM, [ EAT(avidin) ] = 20 µM (for d ), [avidin] = 20 µM (for d ), [ EAT(DNP IgG) ] = 15 µM (for e ), [anti-DNP IgG] = 15 µM (for e ), 100 mM HEPES, pH 8.0. The data represent the mean ± standard deviation.

    Article Snippet: Determination of the activity of RNase AThe enzymatic activity of RNase A was monitored by DNase+RNase detection kit (Jena Bioscience).

    Techniques: Confocal Laser Scanning Microscopy, Avidin-Biotin Assay, Standard Deviation