rnase inhibitor  (Jena Bioscience)


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    Name:
    RNase I
    Description:

    Catalog Number:
    EN-176L
    Price:
    134.3
    Category:
    Molecular Biology
    Size:
    5 x 2000 units
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    Structured Review

    Jena Bioscience rnase inhibitor
    The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and <t>RNase</t> inhibitor. Yellow colour – positive result – presence of <t>RNA,</t> pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.

    https://www.bioz.com/result/rnase inhibitor/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva"

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva

    Journal: Microbial Biotechnology

    doi: 10.1111/1751-7915.13737

    The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.
    Figure Legend Snippet: The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.

    Techniques Used: Positive Control, Negative Control

    Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.
    Figure Legend Snippet: Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.

    Techniques Used: Serial Dilution, Negative Control

    2) Product Images from "Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva"

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva

    Journal: Microbial Biotechnology

    doi: 10.1111/1751-7915.13737

    The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.
    Figure Legend Snippet: The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.

    Techniques Used: Positive Control, Negative Control

    Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.
    Figure Legend Snippet: Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.

    Techniques Used: Serial Dilution, Negative Control

    3) Product Images from "Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva"

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva

    Journal: Microbial Biotechnology

    doi: 10.1111/1751-7915.13737

    The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.
    Figure Legend Snippet: The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.

    Techniques Used: Positive Control, Negative Control

    Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.
    Figure Legend Snippet: Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.

    Techniques Used: Serial Dilution, Negative Control

    4) Product Images from "Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva"

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva

    Journal: Microbial Biotechnology

    doi: 10.1111/1751-7915.13737

    The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.
    Figure Legend Snippet: The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.

    Techniques Used: Positive Control, Negative Control

    Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.
    Figure Legend Snippet: Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.

    Techniques Used: Serial Dilution, Negative Control

    Related Articles

    other:

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva
    Article Snippet: Successful detection of the SARS‐CoV‐2 RNA was possible after addition of the RNase inhibitor with very high sensitivity.

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva
    Article Snippet: While saliva from both interfered with RT‐LAMP, RNase inhibitor was able to prevent the interference from saliva collected using cotton Salivette, but not using polyester‐based Salivette cortisol.

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva
    Article Snippet: We obtained positive results for whole saliva samples where the limit of detection after addition of the RNase inhibitor was six copies of RNA per reaction and for Salivette® samples 12 copies of RNA per reaction (Fig. ).

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva
    Article Snippet: RNase inhibitor (8U, Jena Bioscience, Jena, Germany) was added as we found that it improved detection of SARS‐CoV‐2 in saliva samples.

    RT Lamp Assay:

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva
    Article Snippet: We have rather tested a different approach based on the premise that saliva as a digestive fluid has strong nuclease and especially RNase activity (Robinovitch et al ., ; Ceder et al ., ). .. Indeed, the addition of the RNase inhibitor improved the sensitivity of the RT‐LAMP assay on spiked saliva samples. .. This indicates that the RNase activity of saliva is one of the major issues in salivary RNA diagnostics (Fábryová and Celec, ; Sullivan et al., ).

    Concentration Assay:

    Article Title: Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling
    Article Snippet: .. Briefly, a standard 25 µl reaction mixture contained in final concentration of 1x AMV/TF1 reaction buffer, 0.2 mM dNTPs mix, 3 mM MgSO4 , 0.1 U AMV reverse transcriptase, 0.1 U Tf1 DNA polymerase, 0.8 U RNase inhibitor (Jena Bioscience Germany), 0.5 µM of each reverse and forward primers GPX R. AGA GCG GGT GAG CCT TCT and F. GGG CAA AGA AGA TTC CAG GTT, GHST R. GTC AGC CTG TTC CCT ACA AG and F. GCC TTC TAC CCG AAG ACA CCT T and CAT R. GTA CGA CTC ACT ATA GGG ACA CGA GGT CCC AGT TAC CAT and F. AGG TGA CAC TAT AGA ATA GTG GTT TTC ACC GAC GAG AT (Jena Bioscience, Germany), 100 ng of cDNA and RNase free water. .. Target genes were amplified in low-profile 0.2 ml tube stripes (Axygen, CA).

    Cellular Antioxidant Activity Assay:

    Article Title: Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling
    Article Snippet: .. Briefly, a standard 25 µl reaction mixture contained in final concentration of 1x AMV/TF1 reaction buffer, 0.2 mM dNTPs mix, 3 mM MgSO4 , 0.1 U AMV reverse transcriptase, 0.1 U Tf1 DNA polymerase, 0.8 U RNase inhibitor (Jena Bioscience Germany), 0.5 µM of each reverse and forward primers GPX R. AGA GCG GGT GAG CCT TCT and F. GGG CAA AGA AGA TTC CAG GTT, GHST R. GTC AGC CTG TTC CCT ACA AG and F. GCC TTC TAC CCG AAG ACA CCT T and CAT R. GTA CGA CTC ACT ATA GGG ACA CGA GGT CCC AGT TAC CAT and F. AGG TGA CAC TAT AGA ATA GTG GTT TTC ACC GAC GAG AT (Jena Bioscience, Germany), 100 ng of cDNA and RNase free water. .. Target genes were amplified in low-profile 0.2 ml tube stripes (Axygen, CA).

    CTG Assay:

    Article Title: Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling
    Article Snippet: .. Briefly, a standard 25 µl reaction mixture contained in final concentration of 1x AMV/TF1 reaction buffer, 0.2 mM dNTPs mix, 3 mM MgSO4 , 0.1 U AMV reverse transcriptase, 0.1 U Tf1 DNA polymerase, 0.8 U RNase inhibitor (Jena Bioscience Germany), 0.5 µM of each reverse and forward primers GPX R. AGA GCG GGT GAG CCT TCT and F. GGG CAA AGA AGA TTC CAG GTT, GHST R. GTC AGC CTG TTC CCT ACA AG and F. GCC TTC TAC CCG AAG ACA CCT T and CAT R. GTA CGA CTC ACT ATA GGG ACA CGA GGT CCC AGT TAC CAT and F. AGG TGA CAC TAT AGA ATA GTG GTT TTC ACC GAC GAG AT (Jena Bioscience, Germany), 100 ng of cDNA and RNase free water. .. Target genes were amplified in low-profile 0.2 ml tube stripes (Axygen, CA).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling
    Article Snippet: .. Briefly, a standard 25 µl reaction mixture contained in final concentration of 1x AMV/TF1 reaction buffer, 0.2 mM dNTPs mix, 3 mM MgSO4 , 0.1 U AMV reverse transcriptase, 0.1 U Tf1 DNA polymerase, 0.8 U RNase inhibitor (Jena Bioscience Germany), 0.5 µM of each reverse and forward primers GPX R. AGA GCG GGT GAG CCT TCT and F. GGG CAA AGA AGA TTC CAG GTT, GHST R. GTC AGC CTG TTC CCT ACA AG and F. GCC TTC TAC CCG AAG ACA CCT T and CAT R. GTA CGA CTC ACT ATA GGG ACA CGA GGT CCC AGT TAC CAT and F. AGG TGA CAC TAT AGA ATA GTG GTT TTC ACC GAC GAG AT (Jena Bioscience, Germany), 100 ng of cDNA and RNase free water. .. Target genes were amplified in low-profile 0.2 ml tube stripes (Axygen, CA).

    Activated Clotting Time Assay:

    Article Title: Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling
    Article Snippet: .. Briefly, a standard 25 µl reaction mixture contained in final concentration of 1x AMV/TF1 reaction buffer, 0.2 mM dNTPs mix, 3 mM MgSO4 , 0.1 U AMV reverse transcriptase, 0.1 U Tf1 DNA polymerase, 0.8 U RNase inhibitor (Jena Bioscience Germany), 0.5 µM of each reverse and forward primers GPX R. AGA GCG GGT GAG CCT TCT and F. GGG CAA AGA AGA TTC CAG GTT, GHST R. GTC AGC CTG TTC CCT ACA AG and F. GCC TTC TAC CCG AAG ACA CCT T and CAT R. GTA CGA CTC ACT ATA GGG ACA CGA GGT CCC AGT TAC CAT and F. AGG TGA CAC TAT AGA ATA GTG GTT TTC ACC GAC GAG AT (Jena Bioscience, Germany), 100 ng of cDNA and RNase free water. .. Target genes were amplified in low-profile 0.2 ml tube stripes (Axygen, CA).

    Countercurrent Chromatography:

    Article Title: Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling
    Article Snippet: .. Briefly, a standard 25 µl reaction mixture contained in final concentration of 1x AMV/TF1 reaction buffer, 0.2 mM dNTPs mix, 3 mM MgSO4 , 0.1 U AMV reverse transcriptase, 0.1 U Tf1 DNA polymerase, 0.8 U RNase inhibitor (Jena Bioscience Germany), 0.5 µM of each reverse and forward primers GPX R. AGA GCG GGT GAG CCT TCT and F. GGG CAA AGA AGA TTC CAG GTT, GHST R. GTC AGC CTG TTC CCT ACA AG and F. GCC TTC TAC CCG AAG ACA CCT T and CAT R. GTA CGA CTC ACT ATA GGG ACA CGA GGT CCC AGT TAC CAT and F. AGG TGA CAC TAT AGA ATA GTG GTT TTC ACC GAC GAG AT (Jena Bioscience, Germany), 100 ng of cDNA and RNase free water. .. Target genes were amplified in low-profile 0.2 ml tube stripes (Axygen, CA).

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  • Team
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  • Bioz Stars
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  • 94
    Jena Bioscience rnase inhibitor
    The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and <t>RNase</t> inhibitor. Yellow colour – positive result – presence of <t>RNA,</t> pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.
    Rnase Inhibitor, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase inhibitor/product/Jena Bioscience
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    Image Search Results


    The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.

    Journal: Microbial Biotechnology

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva

    doi: 10.1111/1751-7915.13737

    Figure Lengend Snippet: The RT‐LAMP on clinical saliva samples performed with Yan et al . (S‐123) primer mix and RNase inhibitor. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. PC, positive control; NC, negative control.

    Article Snippet: We obtained positive results for whole saliva samples where the limit of detection after addition of the RNase inhibitor was six copies of RNA per reaction and for Salivette® samples 12 copies of RNA per reaction (Fig. ).

    Techniques: Positive Control, Negative Control

    Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.

    Journal: Microbial Biotechnology

    Article Title: Loop‐mediated isothermal amplification for the detection of SARS‐CoV‐2 in saliva

    doi: 10.1111/1751-7915.13737

    Figure Lengend Snippet: Effect of RNase inhibitor on RT‐LAMP on saliva samples with a serial dilution of the EDX SARS‐CoV‐2 RNA Standard as the RNA template performed with Yan et al . (S‐123) primer mix. Yellow colour – positive result – presence of RNA, pink colour – negative result – absence of RNA. NC, negative control.

    Article Snippet: We obtained positive results for whole saliva samples where the limit of detection after addition of the RNase inhibitor was six copies of RNA per reaction and for Salivette® samples 12 copies of RNA per reaction (Fig. ).

    Techniques: Serial Dilution, Negative Control