nhei restriction enzymes  (Jena Bioscience)


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  • 85
    Name:
    NheI
    Description:

    Catalog Number:
    en-146l
    Price:
    None
    Size:
    5 x 550 Units
    Category:
    Molecular Biology
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    Structured Review

    Jena Bioscience nhei restriction enzymes
    Digestion by <t>NheI</t> and <t>SalI</t> restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)

    https://www.bioz.com/result/nhei restriction enzymes/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhei restriction enzymes - by Bioz Stars, 2021-02
    85/100 stars

    Images

    1) Product Images from "Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay"

    Article Title: Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay

    Journal: Iranian Journal of Public Health

    doi:

    Digestion by NheI and SalI restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)
    Figure Legend Snippet: Digestion by NheI and SalI restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)

    Techniques Used: Marker, Polymerase Chain Reaction

    Related Articles

    Clone Assay:

    Article Title: Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay
    Article Snippet: .. The synthesized gene was amplified by M13 primers ( ) and digested by using SalI and NheI restriction enzymes then sub cloned into pLEXSY-hyg2 plasmid that encoded 6His tag gene (EGE-232, Jena Bioscience, Germany). .. Recombinant pLEXSY-hyg-2-hAm was transformed into E. coli XL1-Blue (stratagene, UK).

    Amplification:

    Article Title: Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay
    Article Snippet: .. The synthesized gene was amplified by M13 primers ( ) and digested by using SalI and NheI restriction enzymes then sub cloned into pLEXSY-hyg2 plasmid that encoded 6His tag gene (EGE-232, Jena Bioscience, Germany). .. Recombinant pLEXSY-hyg-2-hAm was transformed into E. coli XL1-Blue (stratagene, UK).

    Plasmid Preparation:

    Article Title: Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay
    Article Snippet: .. The synthesized gene was amplified by M13 primers ( ) and digested by using SalI and NheI restriction enzymes then sub cloned into pLEXSY-hyg2 plasmid that encoded 6His tag gene (EGE-232, Jena Bioscience, Germany). .. Recombinant pLEXSY-hyg-2-hAm was transformed into E. coli XL1-Blue (stratagene, UK).

    Synthesized:

    Article Title: Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay
    Article Snippet: .. The synthesized gene was amplified by M13 primers ( ) and digested by using SalI and NheI restriction enzymes then sub cloned into pLEXSY-hyg2 plasmid that encoded 6His tag gene (EGE-232, Jena Bioscience, Germany). .. Recombinant pLEXSY-hyg-2-hAm was transformed into E. coli XL1-Blue (stratagene, UK).

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  • 85
    Jena Bioscience nhei restriction enzymes
    Digestion by <t>NheI</t> and <t>SalI</t> restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)
    Nhei Restriction Enzymes, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei restriction enzymes/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhei restriction enzymes - by Bioz Stars, 2021-02
    85/100 stars
      Buy from Supplier

    Image Search Results


    Digestion by NheI and SalI restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)

    Journal: Iranian Journal of Public Health

    Article Title: Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay

    doi:

    Figure Lengend Snippet: Digestion by NheI and SalI restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)

    Article Snippet: The synthesized gene was amplified by M13 primers ( ) and digested by using SalI and NheI restriction enzymes then sub cloned into pLEXSY-hyg2 plasmid that encoded 6His tag gene (EGE-232, Jena Bioscience, Germany).

    Techniques: Marker, Polymerase Chain Reaction