restriction enzymes bglii  (Jena Bioscience)


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    Name:
    BglII
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    Catalog Number:
    en-106l
    Price:
    114.8
    Category:
    Molecular Biology
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    Structured Review

    Jena Bioscience restriction enzymes bglii

    https://www.bioz.com/result/restriction enzymes bglii/product/Jena Bioscience
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bglii - by Bioz Stars, 2020-09
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A non-pathogenic live vector as an efficient delivery system in vaccine design for the prevention of HPV16 E7-overexpressing cancers.
    Article Snippet: .. Plasmid DNA constructs and preparation For the generation of E7-NT (gp96)-GFP-expressing plasmid [pLEXSY-E7-NT (gp96)-GFP], the E7-NT (gp96)-GFP gene was sub-cloned from pcDNA-E7-NT (gp96)-GFP (previously provided by our groups in Molecular Immunology and Vaccine Research Lab.) into the BglII and KpnI cloning sites of pLEXSY-neo2 expression vector (Jena bioscience). .. Furthermore, to make the E7-CT (gp96)-GFP-expressing plasmid [pLEXSY-E7-CT (gp96)-GFP], the E7-CT (gp96) gene was sub-cloned from pUC-E7-CT (gp96) into the BglII and KpnI cloning sites of pLEXSY-GFP expression vector.

    Article Title: Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae.
    Article Snippet: .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway. .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway.

    Amplification:

    Article Title: Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae.
    Article Snippet: .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway. .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway.

    Article Title: Cloning, overexpression, and purification of glucose-6-phosphate dehydrogenase of Pseudomonas aeruginosa.
    Article Snippet: .. Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.363) plays an important role in the human pathogen Pseudomonas aeruginosa because it generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes. .. Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.363) plays an important role in the human pathogen Pseudomonas aeruginosa because it generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes.

    Construct:

    Article Title: A non-pathogenic live vector as an efficient delivery system in vaccine design for the prevention of HPV16 E7-overexpressing cancers.
    Article Snippet: .. Plasmid DNA constructs and preparation For the generation of E7-NT (gp96)-GFP-expressing plasmid [pLEXSY-E7-NT (gp96)-GFP], the E7-NT (gp96)-GFP gene was sub-cloned from pcDNA-E7-NT (gp96)-GFP (previously provided by our groups in Molecular Immunology and Vaccine Research Lab.) into the BglII and KpnI cloning sites of pLEXSY-neo2 expression vector (Jena bioscience). .. Furthermore, to make the E7-CT (gp96)-GFP-expressing plasmid [pLEXSY-E7-CT (gp96)-GFP], the E7-CT (gp96) gene was sub-cloned from pUC-E7-CT (gp96) into the BglII and KpnI cloning sites of pLEXSY-GFP expression vector.

    Purification:

    Article Title: Fluorescent Leishmania species: development of stable GFP expression and its application for in vitro and in vivo studies.
    Article Snippet: .. Reporter genes have proved to be an excellent tool for studying disease progression. .. Reporter genes have proved to be an excellent tool for studying disease progression.

    Article Title: Cloning, overexpression, and purification of glucose-6-phosphate dehydrogenase of Pseudomonas aeruginosa.
    Article Snippet: .. Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.363) plays an important role in the human pathogen Pseudomonas aeruginosa because it generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes. .. Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.363) plays an important role in the human pathogen Pseudomonas aeruginosa because it generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes.

    Polymerase Chain Reaction:

    Article Title: Chromatin-associated protein complexes link DNA base J and transcription termination in Leishmania
    Article Snippet: .. Following cleavage with BamHI and NotI, the PCR fragment was inserted into BglII+Not1-digested pLEXSY-I-bleCherry3 (Jena Biosciences). .. The resulting plasmid (pLEXSY-MHTAP) allows the TAP-tagging of introduced coding regions under the control of a Tet-regulated T7 promoter, and insertion into the ODC locus on chromosome 12 of L. tarentolae .

    Article Title: Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis
    Article Snippet: .. PCR product was cut with appropriate restriction enzymes and ligated into the Bgl II and Not I sites of the pLEXSY-hyg2 expression vector (Jena Bioscience GmbH, Germany). .. Parasites expressing mCherry Open Reading Frame (ORF) were obtained by transfection of L. major with the large Swa I targeting fragment derived from pLEXSY-mCherry by electroporation and subsequent plating on semisolid media containing 200 µg/ml hygromycin B (Sigma-Aldrich) as previously described .

    Article Title: Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5' sequence context.
    Article Snippet: .. To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of expression plasmids randomized in the three nucleotides prefacing ATG of a reporter gene encoding enhanced green fluorescent protein (EGFP). .. To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of expression plasmids randomized in the three nucleotides prefacing ATG of a reporter gene encoding enhanced green fluorescent protein (EGFP).

    Article Title: Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae.
    Article Snippet: .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway. .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway.

    Article Title: Fluorescent Leishmania species: development of stable GFP expression and its application for in vitro and in vivo studies.
    Article Snippet: .. Reporter genes have proved to be an excellent tool for studying disease progression. .. Reporter genes have proved to be an excellent tool for studying disease progression.

    Expressing:

    Article Title: A non-pathogenic live vector as an efficient delivery system in vaccine design for the prevention of HPV16 E7-overexpressing cancers.
    Article Snippet: .. Plasmid DNA constructs and preparation For the generation of E7-NT (gp96)-GFP-expressing plasmid [pLEXSY-E7-NT (gp96)-GFP], the E7-NT (gp96)-GFP gene was sub-cloned from pcDNA-E7-NT (gp96)-GFP (previously provided by our groups in Molecular Immunology and Vaccine Research Lab.) into the BglII and KpnI cloning sites of pLEXSY-neo2 expression vector (Jena bioscience). .. Furthermore, to make the E7-CT (gp96)-GFP-expressing plasmid [pLEXSY-E7-CT (gp96)-GFP], the E7-CT (gp96) gene was sub-cloned from pUC-E7-CT (gp96) into the BglII and KpnI cloning sites of pLEXSY-GFP expression vector.

    Article Title: Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis
    Article Snippet: .. PCR product was cut with appropriate restriction enzymes and ligated into the Bgl II and Not I sites of the pLEXSY-hyg2 expression vector (Jena Bioscience GmbH, Germany). .. Parasites expressing mCherry Open Reading Frame (ORF) were obtained by transfection of L. major with the large Swa I targeting fragment derived from pLEXSY-mCherry by electroporation and subsequent plating on semisolid media containing 200 µg/ml hygromycin B (Sigma-Aldrich) as previously described .

    Article Title: Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5' sequence context.
    Article Snippet: .. To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of expression plasmids randomized in the three nucleotides prefacing ATG of a reporter gene encoding enhanced green fluorescent protein (EGFP). .. To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of expression plasmids randomized in the three nucleotides prefacing ATG of a reporter gene encoding enhanced green fluorescent protein (EGFP).

    Article Title: Fluorescent Leishmania species: development of stable GFP expression and its application for in vitro and in vivo studies.
    Article Snippet: .. Reporter genes have proved to be an excellent tool for studying disease progression. .. Reporter genes have proved to be an excellent tool for studying disease progression.

    Sequencing:

    Article Title: Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae.
    Article Snippet: .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway. .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway.

    Gel Extraction:

    Article Title: Fluorescent Leishmania species: development of stable GFP expression and its application for in vitro and in vivo studies.
    Article Snippet: .. Reporter genes have proved to be an excellent tool for studying disease progression. .. Reporter genes have proved to be an excellent tool for studying disease progression.

    Article Title: Cloning, overexpression, and purification of glucose-6-phosphate dehydrogenase of Pseudomonas aeruginosa.
    Article Snippet: .. Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.363) plays an important role in the human pathogen Pseudomonas aeruginosa because it generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes. .. Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.363) plays an important role in the human pathogen Pseudomonas aeruginosa because it generates NADPH, an essential cofactor for several biosynthetic pathways and antioxidant enzymes.

    Plasmid Preparation:

    Article Title: A non-pathogenic live vector as an efficient delivery system in vaccine design for the prevention of HPV16 E7-overexpressing cancers.
    Article Snippet: .. Plasmid DNA constructs and preparation For the generation of E7-NT (gp96)-GFP-expressing plasmid [pLEXSY-E7-NT (gp96)-GFP], the E7-NT (gp96)-GFP gene was sub-cloned from pcDNA-E7-NT (gp96)-GFP (previously provided by our groups in Molecular Immunology and Vaccine Research Lab.) into the BglII and KpnI cloning sites of pLEXSY-neo2 expression vector (Jena bioscience). .. Furthermore, to make the E7-CT (gp96)-GFP-expressing plasmid [pLEXSY-E7-CT (gp96)-GFP], the E7-CT (gp96) gene was sub-cloned from pUC-E7-CT (gp96) into the BglII and KpnI cloning sites of pLEXSY-GFP expression vector.

    Article Title: Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis
    Article Snippet: .. PCR product was cut with appropriate restriction enzymes and ligated into the Bgl II and Not I sites of the pLEXSY-hyg2 expression vector (Jena Bioscience GmbH, Germany). .. Parasites expressing mCherry Open Reading Frame (ORF) were obtained by transfection of L. major with the large Swa I targeting fragment derived from pLEXSY-mCherry by electroporation and subsequent plating on semisolid media containing 200 µg/ml hygromycin B (Sigma-Aldrich) as previously described .

    Article Title: Development of Novel Prime-Boost Strategies Based on a Tri-Gene Fusion Recombinant L. tarentolae Vaccine against Experimental Murine Visceral Leishmaniasis
    Article Snippet: .. Then, the A2-CPA-CPB-CTE -GFP fragment was subcloned into the Bgl II and Not I sites of vector pLEXSY-NEO2 (EGE-233, Jena Bioscience, Germany) to generate pLEXSY-A2-CPA-CPB-CTE -GFP. .. Also the A2-CPA-CPB-CTE fragment was subcloned into the Eco RI and Bam HI sites of vector pcDNA3.1(−) (Invitrogen, Germany) to generate pcDNA-A2-CPA-CPB-CTE as a DNA vaccine. pcDNA-A2-CPA-CPB-CTE plasmid was purified by ion exchange chromatography with Endofree Mega kit (QIAGEN, Germany).

    Article Title: Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5' sequence context.
    Article Snippet: .. To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of expression plasmids randomized in the three nucleotides prefacing ATG of a reporter gene encoding enhanced green fluorescent protein (EGFP). .. To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of expression plasmids randomized in the three nucleotides prefacing ATG of a reporter gene encoding enhanced green fluorescent protein (EGFP).

    Article Title: Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae.
    Article Snippet: .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway. .. N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway.

    Article Title: Fluorescent Leishmania species: development of stable GFP expression and its application for in vitro and in vivo studies.
    Article Snippet: .. Reporter genes have proved to be an excellent tool for studying disease progression. .. Reporter genes have proved to be an excellent tool for studying disease progression.

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  • 94
    Jena Bioscience restriction enzymes bglii
    Restriction Enzymes Bglii, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bglii/product/Jena Bioscience
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bglii - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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