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NanoSight emvs
NTA and TEM of 143B <t>EMVs.</t> A shows NTA measurements of size and concentration of EMVs in three different samples. B shows a screenshot of video from NanoSight LM14 showing light scatter caused by 143 EMVs. C and D show electron micrographs of 143B <t>EMV</t>
Emvs, supplied by NanoSight, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
emvs - by Bioz Stars, 2020-09
88/100 stars

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1) Product Images from "Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment"

Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment

Journal: Translational Oncology

doi: 10.1016/j.tranon.2014.04.011

NTA and TEM of 143B EMVs. A shows NTA measurements of size and concentration of EMVs in three different samples. B shows a screenshot of video from NanoSight LM14 showing light scatter caused by 143 EMVs. C and D show electron micrographs of 143B EMV
Figure Legend Snippet: NTA and TEM of 143B EMVs. A shows NTA measurements of size and concentration of EMVs in three different samples. B shows a screenshot of video from NanoSight LM14 showing light scatter caused by 143 EMVs. C and D show electron micrographs of 143B EMV

Techniques Used: Transmission Electron Microscopy, Concentration Assay

Comparison of EMV output between 143B, an aggressive osteosarcoma cell line versus HOS, a nonaggressive nonmetastatic cell line. A shows the number of EMVs (× 10 8 /ml) as determined by NTA, and B compares the protein concentration (mg/ml) of EMVs
Figure Legend Snippet: Comparison of EMV output between 143B, an aggressive osteosarcoma cell line versus HOS, a nonaggressive nonmetastatic cell line. A shows the number of EMVs (× 10 8 /ml) as determined by NTA, and B compares the protein concentration (mg/ml) of EMVs

Techniques Used: Protein Concentration

Related Articles

Electron Microscopy:

Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment
Article Snippet: .. We thank Shrikant Anant for access to ultracentrifuge for isolation of EMVs and helpful suggestions, Lane Christenson and Peggy Petroff for allowing us to use the NanoSight equipment for NTA, Jeremy Chien for access to the ChemiDoc MP system, Marsha Danley for helping with IHC, Barbara Fegley for assistance with the electron microscopy at the Electron Microscopy Research Laboratory, which is supported, in part, by funds from NIH Centers of Biomedical Research Excellence (COBRE) grant 9P20GM104936 and NIH grant S10RR027564. ..

Immunohistochemistry:

Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment
Article Snippet: .. We thank Shrikant Anant for access to ultracentrifuge for isolation of EMVs and helpful suggestions, Lane Christenson and Peggy Petroff for allowing us to use the NanoSight equipment for NTA, Jeremy Chien for access to the ChemiDoc MP system, Marsha Danley for helping with IHC, Barbara Fegley for assistance with the electron microscopy at the Electron Microscopy Research Laboratory, which is supported, in part, by funds from NIH Centers of Biomedical Research Excellence (COBRE) grant 9P20GM104936 and NIH grant S10RR027564. ..

Software:

Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment
Article Snippet: .. To determine the EMV concentration and size distribution profile of EMVs isolated from CM of osteosarcoma cell cultures, vesicles were analyzed using the NanoSight (Amesbury, UK) NTA 2.3: Nanoparticle Tracking and Analysis instrument and software (release version build 11 RC1, 2012, hardware: LM14). ..

Isolation:

Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment
Article Snippet: .. To determine the EMV concentration and size distribution profile of EMVs isolated from CM of osteosarcoma cell cultures, vesicles were analyzed using the NanoSight (Amesbury, UK) NTA 2.3: Nanoparticle Tracking and Analysis instrument and software (release version build 11 RC1, 2012, hardware: LM14). ..

Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment
Article Snippet: .. We thank Shrikant Anant for access to ultracentrifuge for isolation of EMVs and helpful suggestions, Lane Christenson and Peggy Petroff for allowing us to use the NanoSight equipment for NTA, Jeremy Chien for access to the ChemiDoc MP system, Marsha Danley for helping with IHC, Barbara Fegley for assistance with the electron microscopy at the Electron Microscopy Research Laboratory, which is supported, in part, by funds from NIH Centers of Biomedical Research Excellence (COBRE) grant 9P20GM104936 and NIH grant S10RR027564. ..

Concentration Assay:

Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment
Article Snippet: .. To determine the EMV concentration and size distribution profile of EMVs isolated from CM of osteosarcoma cell cultures, vesicles were analyzed using the NanoSight (Amesbury, UK) NTA 2.3: Nanoparticle Tracking and Analysis instrument and software (release version build 11 RC1, 2012, hardware: LM14). ..

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    NanoSight emvs
    NTA and TEM of 143B <t>EMVs.</t> A shows NTA measurements of size and concentration of EMVs in three different samples. B shows a screenshot of video from NanoSight LM14 showing light scatter caused by 143 EMVs. C and D show electron micrographs of 143B <t>EMV</t>
    Emvs, supplied by NanoSight, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/emvs/product/NanoSight
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    emvs - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

    86
    NanoSight urinary emv
    Performance Characterization using Isolated Urinary <t>EMV.</t> A. Urinary EMV were isolated from human urine using a differential centrifugation method and analyzed by <t>nanoparticle</t> tracking analysis as described in Materials and Methods . B–D. Urinary EMV was diluted in phosphate buffer saline (PBS) with different EMV concentrations (B), pH (C) or buffer strength (D), then applied to the filter material. ACTB (○) and GAPDH (□) were quantified in triplicate as described in Materials and Methods . Mean Ct values were plotted and error bars are standard deviations.
    Urinary Emv, supplied by NanoSight, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/urinary emv/product/NanoSight
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    urinary emv - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    NTA and TEM of 143B EMVs. A shows NTA measurements of size and concentration of EMVs in three different samples. B shows a screenshot of video from NanoSight LM14 showing light scatter caused by 143 EMVs. C and D show electron micrographs of 143B EMV

    Journal: Translational Oncology

    Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment

    doi: 10.1016/j.tranon.2014.04.011

    Figure Lengend Snippet: NTA and TEM of 143B EMVs. A shows NTA measurements of size and concentration of EMVs in three different samples. B shows a screenshot of video from NanoSight LM14 showing light scatter caused by 143 EMVs. C and D show electron micrographs of 143B EMV

    Article Snippet: To determine the EMV concentration and size distribution profile of EMVs isolated from CM of osteosarcoma cell cultures, vesicles were analyzed using the NanoSight (Amesbury, UK) NTA 2.3: Nanoparticle Tracking and Analysis instrument and software (release version build 11 RC1, 2012, hardware: LM14).

    Techniques: Transmission Electron Microscopy, Concentration Assay

    Comparison of EMV output between 143B, an aggressive osteosarcoma cell line versus HOS, a nonaggressive nonmetastatic cell line. A shows the number of EMVs (× 10 8 /ml) as determined by NTA, and B compares the protein concentration (mg/ml) of EMVs

    Journal: Translational Oncology

    Article Title: Extracellular Membrane Vesicles Derived from 143B Osteosarcoma Cells Contain Pro-Osteoclastogenic Cargo: A Novel Communication Mechanism in Osteosarcoma Bone Microenvironment

    doi: 10.1016/j.tranon.2014.04.011

    Figure Lengend Snippet: Comparison of EMV output between 143B, an aggressive osteosarcoma cell line versus HOS, a nonaggressive nonmetastatic cell line. A shows the number of EMVs (× 10 8 /ml) as determined by NTA, and B compares the protein concentration (mg/ml) of EMVs

    Article Snippet: To determine the EMV concentration and size distribution profile of EMVs isolated from CM of osteosarcoma cell cultures, vesicles were analyzed using the NanoSight (Amesbury, UK) NTA 2.3: Nanoparticle Tracking and Analysis instrument and software (release version build 11 RC1, 2012, hardware: LM14).

    Techniques: Protein Concentration

    Pharmacological inhibition of EMV release from PC3 cells is highest with Cl-amidine, bisindolylmaleimide-I, and imipramine. ( A ) Using NTA, the most significant inhibition of EMV release from PC3 cells after 24 h was observed in the presence of Cl-amidine, bisindolylmaleimide-I, and imipramine. After 24 h, none of the inhibitors caused any significant reduction in cell viability, ( B ) except for d -pantethine. The experiments were repeated three times, and the data presented are mean ± SEM of the results. (* p ≤ 0.05; **** p ≤ 0.0001).

    Journal: International Journal of Molecular Sciences

    Article Title: Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy

    doi: 10.3390/ijms18051007

    Figure Lengend Snippet: Pharmacological inhibition of EMV release from PC3 cells is highest with Cl-amidine, bisindolylmaleimide-I, and imipramine. ( A ) Using NTA, the most significant inhibition of EMV release from PC3 cells after 24 h was observed in the presence of Cl-amidine, bisindolylmaleimide-I, and imipramine. After 24 h, none of the inhibitors caused any significant reduction in cell viability, ( B ) except for d -pantethine. The experiments were repeated three times, and the data presented are mean ± SEM of the results. (* p ≤ 0.05; **** p ≤ 0.0001).

    Article Snippet: In summary, Cl-amidine, and bisindolylmaleimide-I gave the highest inhibition of EMV release followed by imipramine and d -pantethine as detected by NanoSight analysis ( and A).

    Techniques: Inhibition

    Cl-amidine and bisindolylmaleimide-1-mediated inhibition of EMV release increases the apoptosis of PC3 and MCF-7 cells treated with 5-FU. The Guava Viacount Cell Death Assay shows that PC3 and MCF-7 cells that were given 5-FU together with Cl-amidine, bisindolylmaleimide-I, or with a combination of Cl-amidine and bisindolylmaleimide-I, had significantly reduced levels of cell viability within 24 h compared to PC3 and MCF-7 cells receiving no EMV inhibitors and given only 5-FU. Bisindolylmaleimide-I and Cl-amidine had no significant effect on cell viability on their own. Data presented are the mean ± SEM of three independent experiments performed in triplicate (** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 were considered statistically significant compared to the drug-treated control in the absence of inhibitors).

    Journal: International Journal of Molecular Sciences

    Article Title: Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy

    doi: 10.3390/ijms18051007

    Figure Lengend Snippet: Cl-amidine and bisindolylmaleimide-1-mediated inhibition of EMV release increases the apoptosis of PC3 and MCF-7 cells treated with 5-FU. The Guava Viacount Cell Death Assay shows that PC3 and MCF-7 cells that were given 5-FU together with Cl-amidine, bisindolylmaleimide-I, or with a combination of Cl-amidine and bisindolylmaleimide-I, had significantly reduced levels of cell viability within 24 h compared to PC3 and MCF-7 cells receiving no EMV inhibitors and given only 5-FU. Bisindolylmaleimide-I and Cl-amidine had no significant effect on cell viability on their own. Data presented are the mean ± SEM of three independent experiments performed in triplicate (** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 were considered statistically significant compared to the drug-treated control in the absence of inhibitors).

    Article Snippet: In summary, Cl-amidine, and bisindolylmaleimide-I gave the highest inhibition of EMV release followed by imipramine and d -pantethine as detected by NanoSight analysis ( and A).

    Techniques: Inhibition

    Nanoparticle tracking analysis (NTA) of exosomes and microvesicles (EMVs) released from PC3 cells in the presence of a range of EMV inhibitors. Plots presenting NTA analysis show the concentration of vesicles (0–900 nm in diameter) released from PC3 cells in the absence of any EMV inhibitors ( A ); In ( B ) the EMVs are shown to comprise exosomes and microvesicles (MVs) by electron microscopy, by Western blotting (for CD63 expression), and by the degree of phosphatidylserine (PS) exposition. NTA analysis for released EMVs from PC3 cells are presented in the presence of Cl-amidine ( C ); bisindolylmaleimide-I ( D ); and imipramine ( E ). Vesicles outside the size range of 0–900 nm were excluded to avoid including larger vesicles such as MV aggregates or apoptotic bodies. The experiment was repeated three times in total (error bars ± SEM, indicated in red).

    Journal: International Journal of Molecular Sciences

    Article Title: Chloramidine/Bisindolylmaleimide-I-Mediated Inhibition of Exosome and Microvesicle Release and Enhanced Efficacy of Cancer Chemotherapy

    doi: 10.3390/ijms18051007

    Figure Lengend Snippet: Nanoparticle tracking analysis (NTA) of exosomes and microvesicles (EMVs) released from PC3 cells in the presence of a range of EMV inhibitors. Plots presenting NTA analysis show the concentration of vesicles (0–900 nm in diameter) released from PC3 cells in the absence of any EMV inhibitors ( A ); In ( B ) the EMVs are shown to comprise exosomes and microvesicles (MVs) by electron microscopy, by Western blotting (for CD63 expression), and by the degree of phosphatidylserine (PS) exposition. NTA analysis for released EMVs from PC3 cells are presented in the presence of Cl-amidine ( C ); bisindolylmaleimide-I ( D ); and imipramine ( E ). Vesicles outside the size range of 0–900 nm were excluded to avoid including larger vesicles such as MV aggregates or apoptotic bodies. The experiment was repeated three times in total (error bars ± SEM, indicated in red).

    Article Snippet: In summary, Cl-amidine, and bisindolylmaleimide-I gave the highest inhibition of EMV release followed by imipramine and d -pantethine as detected by NanoSight analysis ( and A).

    Techniques: Concentration Assay, Electron Microscopy, Western Blot, Expressing

    Performance Characterization using Isolated Urinary EMV. A. Urinary EMV were isolated from human urine using a differential centrifugation method and analyzed by nanoparticle tracking analysis as described in Materials and Methods . B–D. Urinary EMV was diluted in phosphate buffer saline (PBS) with different EMV concentrations (B), pH (C) or buffer strength (D), then applied to the filter material. ACTB (○) and GAPDH (□) were quantified in triplicate as described in Materials and Methods . Mean Ct values were plotted and error bars are standard deviations.

    Journal: PLoS ONE

    Article Title: Development of Glomerulus-, Tubule-, and Collecting Duct-Specific mRNA Assay in Human Urinary Exosomes and Microvesicles

    doi: 10.1371/journal.pone.0109074

    Figure Lengend Snippet: Performance Characterization using Isolated Urinary EMV. A. Urinary EMV were isolated from human urine using a differential centrifugation method and analyzed by nanoparticle tracking analysis as described in Materials and Methods . B–D. Urinary EMV was diluted in phosphate buffer saline (PBS) with different EMV concentrations (B), pH (C) or buffer strength (D), then applied to the filter material. ACTB (○) and GAPDH (□) were quantified in triplicate as described in Materials and Methods . Mean Ct values were plotted and error bars are standard deviations.

    Article Snippet: Nanoparticle tracking analysis of the obtained urinary EMV was conducted by Nanosight LM20 (Nanosight, Novato, CA).

    Techniques: Isolation, Centrifugation