emv free foetal bovine serum fbs  (Millipore)


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    Name:
    Bovine serum
    Description:
    Bovine serum is a pooled serum sample obtained from a normal donor herd It is specific for anti bovine serum and yields multiple arcs of precipitation during immunoelectrophoresis versus anti bovine serum Bovine serum has not been assayed for the presence of endogenous antibodies
    Catalog Number:
    b8655
    Price:
    None
    Applications:
    Normal bovine serum is generally used as a negative control or blocking agent in immunoassays. It may also be used as controls for gas chromatography/ high-resolution mass spectrometry analysis .
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    Structured Review

    Millipore emv free foetal bovine serum fbs
    Bovine serum is a pooled serum sample obtained from a normal donor herd It is specific for anti bovine serum and yields multiple arcs of precipitation during immunoelectrophoresis versus anti bovine serum Bovine serum has not been assayed for the presence of endogenous antibodies
    https://www.bioz.com/result/emv free foetal bovine serum fbs/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    emv free foetal bovine serum fbs - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Infection:

    Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
    Article Snippet: .. The membrane was incubated with infected PmCQ2 bovine serum (PmCQ2-IS; 1:16) and horseradish peroxidase-conjugated rabbit anti-bovine IgG antibodies (cat. no. SAB3700018; 1:20,000; Sigma-Aldrich; Merck KGaA). .. An Enhanced Chemiluminescent reagent (Pierce; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was applied for a chromogenic reaction.

    Incubation:

    Article Title: Two novel cross-protective antigens for bovine Pasteurella multocida
    Article Snippet: .. The membrane was incubated with infected PmCQ2 bovine serum (PmCQ2-IS; 1:16) and horseradish peroxidase-conjugated rabbit anti-bovine IgG antibodies (cat. no. SAB3700018; 1:20,000; Sigma-Aldrich; Merck KGaA). .. An Enhanced Chemiluminescent reagent (Pierce; Thermo Fisher Scientific, Inc., Waltham, MA, USA) was applied for a chromogenic reaction.

    other:

    Article Title: Formation of cholesterol ozonolysis products in vitro and in vivo through a myeloperoxidase-dependent pathway
    Article Snippet: Allopurinol, apocynin, cholesterol, 3,4-13 C-cholesterol, catalase (bovine), cell permeable catalase, covalently linked to polyethylene glycol (PEG-catalase), superoxide dismutase (SOD) (bovine), PEG-SOD, PMA, immunoglobulin G (bovine serum), p -toluenesulfonic acid, sodium azide, and LDL were purchased from Sigma, St. Louis, MO.

    Article Title: Activation of factor XI by products of prothrombin activation
    Article Snippet: Others included the following: FVIIa, FIXa, FXa, FXI, FXIa, α-IIa, β-IIa, Phe-Pro-Arg-chloromethylketone (FPR-CK), Phe-Phe-Arg-chloromethylketone (FFR-CK) (Haematologic Technologies). β-IIa was prepared by limited digestion of α-IIa with bovine trypsin using the method of Braun et al. Others included: Argatroban (GlaxoSmithKline), Lepirudin (hirudin) (Bayer), dextran sulfate (average molecular mass, 500 000 Da), hirugen, bovine serum albumin, bovine trypsin, Oxyuranus scutellatus venom, Echis carinatus venom (ecarin), rabbit brain cephalin, ovomucoid trypsin inhibitor (OTI), soybean trypsin inhibitor, and phenylmethylsulfonyl fluoride (Sigma-Aldrich); S-2366 (L-pyro-Glu-L-Pro-L-Arg- p -nitroanilide), S-2302 (H-D-Pro-L-Phe-L-Arg- p -nitroanilide, S-2238 (H-D-Phe-L-Pip-L-Arg- p -nitroanilide), DiaPharma.

    Inhibition:

    Article Title: Behavioral, Pharmacological, and Immunological Abnormalities after Streptococcal Exposure: A Novel Rat Model of Sydenham Chorea and Related Neuropsychiatric Disorders
    Article Snippet: .. IgG inhibition was performed by adding either anti-rat IgG agarose beads (Sigma, St Louis, MO) or control bovine serum albumin-agarose beads (Sigma) to experimental (GAS) sera (see ). ..

    Derivative Assay:

    Article Title: Exosomes mediated pentose phosphate pathway in ovarian cancer metastasis: a proteomics analysis
    Article Snippet: .. All fetal bovine serum derived exosomes had been depleted by ultracentrifugation at 120,000 g overnight, and then filtrated through a 0.2 um filter (Millipore, USA). ..

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  • 90
    Millipore guava viacount assay
    CBD does not affect cell viability of PC3, HEPG2, and MDA-MB-231 cells after 1 h treatment. The Guava EasyCyte 8HT flow cytometer (Millipore) and <t>ViaCount</t> assay were used to count and determine viability of CBD treated cells compared to EMV inhibitor Cl-amidine and DMSO treated control cells after 1 h incubation ( ∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).
    Guava Viacount Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guava viacount assay/product/Millipore
    Average 90 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    guava viacount assay - by Bioz Stars, 2021-01
    90/100 stars
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    CBD does not affect cell viability of PC3, HEPG2, and MDA-MB-231 cells after 1 h treatment. The Guava EasyCyte 8HT flow cytometer (Millipore) and ViaCount assay were used to count and determine viability of CBD treated cells compared to EMV inhibitor Cl-amidine and DMSO treated control cells after 1 h incubation ( ∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Cannabidiol (CBD) Is a Novel Inhibitor for Exosome and Microvesicle (EMV) Release in Cancer

    doi: 10.3389/fphar.2018.00889

    Figure Lengend Snippet: CBD does not affect cell viability of PC3, HEPG2, and MDA-MB-231 cells after 1 h treatment. The Guava EasyCyte 8HT flow cytometer (Millipore) and ViaCount assay were used to count and determine viability of CBD treated cells compared to EMV inhibitor Cl-amidine and DMSO treated control cells after 1 h incubation ( ∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Article Snippet: The cells were split every 3–5 days, depending on confluence, washed twice with EMV-free Dulbecco’s Phosphate Buffered Saline (DPBS), prepared as described before ( ) and detached by incubation for 10–15 min at 37o C with 0.25% (v/v) trypsin/EDTA, followed by two washes by centrifugation using EMV-free DPBS at 200 g /5 min. Before the start of every experiment, cell numbers and viability were determined by Guava ViaCount assay (Guava Millipore) and exponentially growing cells with viabilities of ≥95% were used.

    Techniques: Multiple Displacement Amplification, Flow Cytometry, Cytometry, Incubation

    CBD does not affect cell viability of PC3, HEPG2, and MDA-MB-231 cells after 1 h treatment. The Guava EasyCyte 8HT flow cytometer (Millipore) and ViaCount assay were used to count and determine viability of CBD treated cells compared to EMV inhibitor Cl-amidine and DMSO treated control cells after 1 h incubation ( ∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Cannabidiol (CBD) Is a Novel Inhibitor for Exosome and Microvesicle (EMV) Release in Cancer

    doi: 10.3389/fphar.2018.00889

    Figure Lengend Snippet: CBD does not affect cell viability of PC3, HEPG2, and MDA-MB-231 cells after 1 h treatment. The Guava EasyCyte 8HT flow cytometer (Millipore) and ViaCount assay were used to count and determine viability of CBD treated cells compared to EMV inhibitor Cl-amidine and DMSO treated control cells after 1 h incubation ( ∗ p ≤ 0.05; ∗∗ p ≤ 0.01; ∗∗∗ p ≤ 0.001).

    Article Snippet: The cells were split every 3–5 days, depending on confluence, washed twice with EMV-free Dulbecco’s Phosphate Buffered Saline (DPBS), prepared as described before ( ) and detached by incubation for 10–15 min at 37o C with 0.25% (v/v) trypsin/EDTA, followed by two washes by centrifugation using EMV-free DPBS at 200 g /5 min. Before the start of every experiment, cell numbers and viability were determined by Guava ViaCount assay (Guava Millipore) and exponentially growing cells with viabilities of ≥95% were used.

    Techniques: Multiple Displacement Amplification, Flow Cytometry, Cytometry, Incubation