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ATCC
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ATCC
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ATCC
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ATCC
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Journal: Journal of Bacteriology
Article Title: A 3D-printed capillary tube holder for high-throughput chemotaxis assays
doi: 10.1128/jb.00384-25
Figure Lengend Snippet: Constructing standard curves. Standard curves were constructed for each species in both 96- and 384-well plates by plotting the lag times of each well by the number of cells in each well. ( A ) Representative growth curves for the twofold serial dilutions of Bacillus subtilis 3610 and Escherichia coli K12 in 96- and 384-well plates. The solid lines indicate the mean of the technical replicates, while the shaded regions represent the 95% CI. Growth curves are colored by dilution with the wells with the highest concentration of bacterial cells shown in the darkest shade. For 96-well plates, three technical replicates were included for each condition. For 384-well plates, eight technical replicates were included for each condition. ( B ) Standard curves are calculated from growth curves by plotting the number of cells in each well against the lag time. Equations for the best fit line and R 2 value are shown in the figure. Results in this figure are representative of two biological replicates, each containing three or eight technical replicates, for 96- or 384-well plates, respectively.
Article Snippet: E. coli K12 (ATCC 25404) and
Techniques: Construct, Concentration Assay