human embryonic kidney hek 293 t cells  (ATCC)


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    ATCC human embryonic kidney hek 293 t cells
    Human Embryonic Kidney Hek 293 T Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human embryonic kidney hek 293  (ATCC)


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    ATCC human embryonic kidney hek 293
    Human Embryonic Kidney Hek 293, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human embryonic kidney hek 293  (ATCC)


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    ATCC human embryonic kidney hek 293
    Human Embryonic Kidney Hek 293, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human embryonic kidney 293 cell line  (ATCC)


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    ATCC human embryonic kidney 293 cell line
    Human Embryonic Kidney 293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human embryonic kidney hek 293 cells  (ATCC)


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    ATCC human embryonic kidney hek 293 cells
    Human Embryonic Kidney Hek 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human embryonic kidney normal hek 293 cells  (ATCC)


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    ATCC human embryonic kidney normal hek 293 cells
    a and b) Show that achillea fragrantissima induced cell cytotoxicity in breast cancer (MCF-7) and normal cells <t>(HEK-293),</t> respectively. c) Shows the production of dead cells beneath achillea fragrantissima administrated. d) The graph illustrates the achillea fragrantissima -induced decrease of cell viability in MCF-7 cells at different time intervals. e) Shows the spectrofluorimeter analysis of the intracellular generation of ROS in MCF-7 cells. f) The images represent the achillea fragrantissima caused cellular structural damages in MCF-7 cells with differenced magnification. Values denoted mean ± SD for three independent performings (n = 3). Values are statistically significant at *** P <0.0001, ** P <0.01, and ns. (0.05 Two Way ANOVA/ Turkey test).
    Human Embryonic Kidney Normal Hek 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney normal hek 293 cells/product/ATCC
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    1) Product Images from "Achillea fragrantissima (Forssk.) Sch.Bip instigates the ROS / FADD/c-PARP expression that triggers apoptosis in breast cancer cell (MCF-7)"

    Article Title: Achillea fragrantissima (Forssk.) Sch.Bip instigates the ROS / FADD/c-PARP expression that triggers apoptosis in breast cancer cell (MCF-7)

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0304072

    a and b) Show that achillea fragrantissima induced cell cytotoxicity in breast cancer (MCF-7) and normal cells (HEK-293), respectively. c) Shows the production of dead cells beneath achillea fragrantissima administrated. d) The graph illustrates the achillea fragrantissima -induced decrease of cell viability in MCF-7 cells at different time intervals. e) Shows the spectrofluorimeter analysis of the intracellular generation of ROS in MCF-7 cells. f) The images represent the achillea fragrantissima caused cellular structural damages in MCF-7 cells with differenced magnification. Values denoted mean ± SD for three independent performings (n = 3). Values are statistically significant at *** P <0.0001, ** P <0.01, and ns. (0.05 Two Way ANOVA/ Turkey test).
    Figure Legend Snippet: a and b) Show that achillea fragrantissima induced cell cytotoxicity in breast cancer (MCF-7) and normal cells (HEK-293), respectively. c) Shows the production of dead cells beneath achillea fragrantissima administrated. d) The graph illustrates the achillea fragrantissima -induced decrease of cell viability in MCF-7 cells at different time intervals. e) Shows the spectrofluorimeter analysis of the intracellular generation of ROS in MCF-7 cells. f) The images represent the achillea fragrantissima caused cellular structural damages in MCF-7 cells with differenced magnification. Values denoted mean ± SD for three independent performings (n = 3). Values are statistically significant at *** P <0.0001, ** P <0.01, and ns. (0.05 Two Way ANOVA/ Turkey test).

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    human embryonic kidney 293 cells  (ATCC)


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    ATCC human embryonic kidney 293 cells
    Human Embryonic Kidney 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney 293 cells/product/ATCC
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    human embryonic kidney 293 t cells  (ATCC)


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    ATCC human embryonic kidney 293 t cells
    Human Embryonic Kidney 293 T Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney 293 t cells/product/ATCC
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    human embryonic kidney 293 cells  (ATCC)


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    ATCC human embryonic kidney 293 cells
    FHL2 may regulate HIF-1 and β-catenin signaling simultaneously through interacting with GSK-3β in the cytoplasm and p300 in the nucleus. a-h NRK-52E cells were used. a-e Cells were transiently transfected with FHL2 expression vector (pFHL2) or empty vector (pcDNA3) for 6 h. a Co-immunoprecipitation (IP) assay reveals that ectopic expression of FHL2 induced FHL2/GSK-3β complex formation. The cell lysate was immunoprecipitated with antibody against GSK-3β or FHL2, followed by immunoblotting (IB) for FHL2 or GSK-3β, respectively. b, c Representative micrographs of immunofluorescence staining display the co-localization of FHL2 with GSK-3β. The enlarged box areas from b are presented in c . Scale bar in b, 25 μm, in c, 15 μm. d, e Ectopic expression of FHL2 led to the inhibition of GSK-3β. d Representative western blot shows the abundance of total GSK-3β and phosphorylated GSK-3β (Ser9). The ratio of phosphorylated GSK-3β (Ser9) to total GSK-3β protein is presented in e . * P < 0.05 ( n = 3). f Western blot analyses show the stabilization of HIF-1α and β-catenin induced by ectopic expression of FHL2 was blocked by GSK-3β activation. Cells were transiently transfected with pFHL2 or pcDNA3 for 6 h, followed by incubation with or without WO/GFX (10µM/10µM) for 12 h. g, h Cells were co-transfected with HRE g or TOPflash h reporter plasmid and either pFHL2 or pcDNA3, followed by incubation with or without WO/GFX (10µM/10µM) for 12 h. Relative luciferase activities are reported. ** P < 0.01 ( n = 3). i-k <t>HEK293</t> cells were used. i Co-immunoprecipitation (IP) assay shows that ectopic expression of FHL2 induced FHL2/p300 complex formation. Cells were transiently co-transfected with myc-tagged p300 expression vector (pp300) and either pFHL2 (DDK-tagged) or pcDNA3. The cell lysate was immunoprecipitated with antibody against myc or DDK, followed by immunoblotting (IB) for DDK or myc, respectively. j, k IP assay reveals that ectopic expression of FHL2 increased the acetylation of HIF-1α j and β‐catenin k by p300. j Cells were transiently transfected with pcDNA3, pFHL2, pp300 or pFHL2 plus pp300 as indicated, and then treated with hypoxia for 12 h. The cell lysate was immunoprecipitated with antibody against HIF-1α, followed by IB for acetylated Lysine. k Cells were transiently co-transfected with active β‐catenin expression vector (pβ‐cat-S675A, Flag-tagged) and pcDNA3, pFHL2, pp300 or pFHL2 plus pp300 as indicated. The cell lysate was immunoprecipitated with antibody against β‐catenin, followed by IB for acetylated Lysine. Representative blots of one ( a, i-k ), two or one ( f ) or three ( d ) independent experiments. Data are mean ± SD of three ( e, g, h ) independent experiments
    Human Embryonic Kidney 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Proximal tubular FHL2, a novel downstream target of hypoxia inducible factor 1, is a protector against ischemic acute kidney injury"

    Article Title: Proximal tubular FHL2, a novel downstream target of hypoxia inducible factor 1, is a protector against ischemic acute kidney injury

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-024-05289-x

    FHL2 may regulate HIF-1 and β-catenin signaling simultaneously through interacting with GSK-3β in the cytoplasm and p300 in the nucleus. a-h NRK-52E cells were used. a-e Cells were transiently transfected with FHL2 expression vector (pFHL2) or empty vector (pcDNA3) for 6 h. a Co-immunoprecipitation (IP) assay reveals that ectopic expression of FHL2 induced FHL2/GSK-3β complex formation. The cell lysate was immunoprecipitated with antibody against GSK-3β or FHL2, followed by immunoblotting (IB) for FHL2 or GSK-3β, respectively. b, c Representative micrographs of immunofluorescence staining display the co-localization of FHL2 with GSK-3β. The enlarged box areas from b are presented in c . Scale bar in b, 25 μm, in c, 15 μm. d, e Ectopic expression of FHL2 led to the inhibition of GSK-3β. d Representative western blot shows the abundance of total GSK-3β and phosphorylated GSK-3β (Ser9). The ratio of phosphorylated GSK-3β (Ser9) to total GSK-3β protein is presented in e . * P < 0.05 ( n = 3). f Western blot analyses show the stabilization of HIF-1α and β-catenin induced by ectopic expression of FHL2 was blocked by GSK-3β activation. Cells were transiently transfected with pFHL2 or pcDNA3 for 6 h, followed by incubation with or without WO/GFX (10µM/10µM) for 12 h. g, h Cells were co-transfected with HRE g or TOPflash h reporter plasmid and either pFHL2 or pcDNA3, followed by incubation with or without WO/GFX (10µM/10µM) for 12 h. Relative luciferase activities are reported. ** P < 0.01 ( n = 3). i-k HEK293 cells were used. i Co-immunoprecipitation (IP) assay shows that ectopic expression of FHL2 induced FHL2/p300 complex formation. Cells were transiently co-transfected with myc-tagged p300 expression vector (pp300) and either pFHL2 (DDK-tagged) or pcDNA3. The cell lysate was immunoprecipitated with antibody against myc or DDK, followed by immunoblotting (IB) for DDK or myc, respectively. j, k IP assay reveals that ectopic expression of FHL2 increased the acetylation of HIF-1α j and β‐catenin k by p300. j Cells were transiently transfected with pcDNA3, pFHL2, pp300 or pFHL2 plus pp300 as indicated, and then treated with hypoxia for 12 h. The cell lysate was immunoprecipitated with antibody against HIF-1α, followed by IB for acetylated Lysine. k Cells were transiently co-transfected with active β‐catenin expression vector (pβ‐cat-S675A, Flag-tagged) and pcDNA3, pFHL2, pp300 or pFHL2 plus pp300 as indicated. The cell lysate was immunoprecipitated with antibody against β‐catenin, followed by IB for acetylated Lysine. Representative blots of one ( a, i-k ), two or one ( f ) or three ( d ) independent experiments. Data are mean ± SD of three ( e, g, h ) independent experiments
    Figure Legend Snippet: FHL2 may regulate HIF-1 and β-catenin signaling simultaneously through interacting with GSK-3β in the cytoplasm and p300 in the nucleus. a-h NRK-52E cells were used. a-e Cells were transiently transfected with FHL2 expression vector (pFHL2) or empty vector (pcDNA3) for 6 h. a Co-immunoprecipitation (IP) assay reveals that ectopic expression of FHL2 induced FHL2/GSK-3β complex formation. The cell lysate was immunoprecipitated with antibody against GSK-3β or FHL2, followed by immunoblotting (IB) for FHL2 or GSK-3β, respectively. b, c Representative micrographs of immunofluorescence staining display the co-localization of FHL2 with GSK-3β. The enlarged box areas from b are presented in c . Scale bar in b, 25 μm, in c, 15 μm. d, e Ectopic expression of FHL2 led to the inhibition of GSK-3β. d Representative western blot shows the abundance of total GSK-3β and phosphorylated GSK-3β (Ser9). The ratio of phosphorylated GSK-3β (Ser9) to total GSK-3β protein is presented in e . * P < 0.05 ( n = 3). f Western blot analyses show the stabilization of HIF-1α and β-catenin induced by ectopic expression of FHL2 was blocked by GSK-3β activation. Cells were transiently transfected with pFHL2 or pcDNA3 for 6 h, followed by incubation with or without WO/GFX (10µM/10µM) for 12 h. g, h Cells were co-transfected with HRE g or TOPflash h reporter plasmid and either pFHL2 or pcDNA3, followed by incubation with or without WO/GFX (10µM/10µM) for 12 h. Relative luciferase activities are reported. ** P < 0.01 ( n = 3). i-k HEK293 cells were used. i Co-immunoprecipitation (IP) assay shows that ectopic expression of FHL2 induced FHL2/p300 complex formation. Cells were transiently co-transfected with myc-tagged p300 expression vector (pp300) and either pFHL2 (DDK-tagged) or pcDNA3. The cell lysate was immunoprecipitated with antibody against myc or DDK, followed by immunoblotting (IB) for DDK or myc, respectively. j, k IP assay reveals that ectopic expression of FHL2 increased the acetylation of HIF-1α j and β‐catenin k by p300. j Cells were transiently transfected with pcDNA3, pFHL2, pp300 or pFHL2 plus pp300 as indicated, and then treated with hypoxia for 12 h. The cell lysate was immunoprecipitated with antibody against HIF-1α, followed by IB for acetylated Lysine. k Cells were transiently co-transfected with active β‐catenin expression vector (pβ‐cat-S675A, Flag-tagged) and pcDNA3, pFHL2, pp300 or pFHL2 plus pp300 as indicated. The cell lysate was immunoprecipitated with antibody against β‐catenin, followed by IB for acetylated Lysine. Representative blots of one ( a, i-k ), two or one ( f ) or three ( d ) independent experiments. Data are mean ± SD of three ( e, g, h ) independent experiments

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Immunoprecipitation, Western Blot, Immunofluorescence, Staining, Inhibition, Activation Assay, Incubation, Luciferase

    human embryonic kidney 293 t cells  (ATCC)


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    ATCC human embryonic kidney 293 t cells
    Human Embryonic Kidney 293 T Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human embryonic kidney hek 293 t cells
    Human Embryonic Kidney Hek 293 T Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human embryonic kidney hek 293
    Human Embryonic Kidney Hek 293, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human embryonic kidney 293 cell line
    Human Embryonic Kidney 293 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC human embryonic kidney hek 293 cells
    Human Embryonic Kidney Hek 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney hek 293 cells/product/ATCC
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    ATCC human embryonic kidney normal hek 293 cells
    a and b) Show that achillea fragrantissima induced cell cytotoxicity in breast cancer (MCF-7) and normal cells <t>(HEK-293),</t> respectively. c) Shows the production of dead cells beneath achillea fragrantissima administrated. d) The graph illustrates the achillea fragrantissima -induced decrease of cell viability in MCF-7 cells at different time intervals. e) Shows the spectrofluorimeter analysis of the intracellular generation of ROS in MCF-7 cells. f) The images represent the achillea fragrantissima caused cellular structural damages in MCF-7 cells with differenced magnification. Values denoted mean ± SD for three independent performings (n = 3). Values are statistically significant at *** P <0.0001, ** P <0.01, and ns. (0.05 Two Way ANOVA/ Turkey test).
    Human Embryonic Kidney Normal Hek 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney normal hek 293 cells/product/ATCC
    Average 86 stars, based on 1 article reviews
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    ATCC human embryonic kidney 293 cells
    a and b) Show that achillea fragrantissima induced cell cytotoxicity in breast cancer (MCF-7) and normal cells <t>(HEK-293),</t> respectively. c) Shows the production of dead cells beneath achillea fragrantissima administrated. d) The graph illustrates the achillea fragrantissima -induced decrease of cell viability in MCF-7 cells at different time intervals. e) Shows the spectrofluorimeter analysis of the intracellular generation of ROS in MCF-7 cells. f) The images represent the achillea fragrantissima caused cellular structural damages in MCF-7 cells with differenced magnification. Values denoted mean ± SD for three independent performings (n = 3). Values are statistically significant at *** P <0.0001, ** P <0.01, and ns. (0.05 Two Way ANOVA/ Turkey test).
    Human Embryonic Kidney 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney 293 cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human embryonic kidney 293 cells - by Bioz Stars, 2024-06
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    ATCC human embryonic kidney 293 t cells
    a and b) Show that achillea fragrantissima induced cell cytotoxicity in breast cancer (MCF-7) and normal cells <t>(HEK-293),</t> respectively. c) Shows the production of dead cells beneath achillea fragrantissima administrated. d) The graph illustrates the achillea fragrantissima -induced decrease of cell viability in MCF-7 cells at different time intervals. e) Shows the spectrofluorimeter analysis of the intracellular generation of ROS in MCF-7 cells. f) The images represent the achillea fragrantissima caused cellular structural damages in MCF-7 cells with differenced magnification. Values denoted mean ± SD for three independent performings (n = 3). Values are statistically significant at *** P <0.0001, ** P <0.01, and ns. (0.05 Two Way ANOVA/ Turkey test).
    Human Embryonic Kidney 293 T Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic kidney 293 t cells/product/ATCC
    Average 86 stars, based on 1 article reviews
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    human embryonic kidney 293 t cells - by Bioz Stars, 2024-06
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    a and b) Show that achillea fragrantissima induced cell cytotoxicity in breast cancer (MCF-7) and normal cells (HEK-293), respectively. c) Shows the production of dead cells beneath achillea fragrantissima administrated. d) The graph illustrates the achillea fragrantissima -induced decrease of cell viability in MCF-7 cells at different time intervals. e) Shows the spectrofluorimeter analysis of the intracellular generation of ROS in MCF-7 cells. f) The images represent the achillea fragrantissima caused cellular structural damages in MCF-7 cells with differenced magnification. Values denoted mean ± SD for three independent performings (n = 3). Values are statistically significant at *** P <0.0001, ** P <0.01, and ns. (0.05 Two Way ANOVA/ Turkey test).

    Journal: PLOS ONE

    Article Title: Achillea fragrantissima (Forssk.) Sch.Bip instigates the ROS / FADD/c-PARP expression that triggers apoptosis in breast cancer cell (MCF-7)

    doi: 10.1371/journal.pone.0304072

    Figure Lengend Snippet: a and b) Show that achillea fragrantissima induced cell cytotoxicity in breast cancer (MCF-7) and normal cells (HEK-293), respectively. c) Shows the production of dead cells beneath achillea fragrantissima administrated. d) The graph illustrates the achillea fragrantissima -induced decrease of cell viability in MCF-7 cells at different time intervals. e) Shows the spectrofluorimeter analysis of the intracellular generation of ROS in MCF-7 cells. f) The images represent the achillea fragrantissima caused cellular structural damages in MCF-7 cells with differenced magnification. Values denoted mean ± SD for three independent performings (n = 3). Values are statistically significant at *** P <0.0001, ** P <0.01, and ns. (0.05 Two Way ANOVA/ Turkey test).

    Article Snippet: The human breast cancer MCF-7 cells and human embryonic kidney normal HEK-293 cells were purchased from American Type of Cell Culture (ATCC), USA.

    Techniques: