elisa  (Thermo Fisher)


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    Name:
    eBioscience ELISA Wash Buffer
    Description:
    The eBioscience ELISA Wash Buffer Powder is a dried powder formulation of phosphate buffered saline with 0 05 Tween 20 Each packet is reconstituted in 1 Liter of distilled deionized water the resulting 1x PBS Tween 20 buffer pH 7 4 is suitable for washing ELISA plate microwells 10 packets of ELISA Wash Buffer Powder are supplied yielding a total of 10 liters of 1x Wash Buffer Reported ApplicationELISA
    Catalog Number:
    00-0400-46
    Price:
    None
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher elisa
    The eBioscience ELISA Wash Buffer Powder is a dried powder formulation of phosphate buffered saline with 0 05 Tween 20 Each packet is reconstituted in 1 Liter of distilled deionized water the resulting 1x PBS Tween 20 buffer pH 7 4 is suitable for washing ELISA plate microwells 10 packets of ELISA Wash Buffer Powder are supplied yielding a total of 10 liters of 1x Wash Buffer Reported ApplicationELISA
    https://www.bioz.com/result/elisa/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Epstein–Barr Virus Lytic Reactivation Induces IgG4 Production by Host B Lymphocytes in Graves' Disease Patients and Controls: A Subset of Graves' Disease Is an IgG4-Related Disease-Like Condition
    Article Snippet: .. Ig measurements in culture medium and serum We measured IgG4 concentrations in the culture medium using an ELISA kit (Affymetrix eBioscience, Vienna, Austria) and IgG concentrations using ELISA (Bethyl No. E80-104, E80-100, E80-108; Montgomery, TX) according to the manufacturer's instructions. ..

    Article Title: The α7 nicotinic acetylcholine receptor positive allosteric modulator prevents lipopolysaccharide-induced allodynia, hyperalgesia and TNF-α in the hippocampus in mice
    Article Snippet: .. Quantification of hippocampal TNF-α was assessed by ELISA kit (eBioscience, San Diego, CA, USA), according to manufacturer’s instructions. .. NE assay NE assay was performed as described previously with minor modification [ ].

    Article Title: Modulation of monosodium urate crystal-induced responses in neutrophils by the myeloid inhibitory C-type lectin-like receptor: potential therapeutic implications
    Article Snippet: Extracellular IL-8 was quantified using commercially available ELISA kits (BD Biosciences). .. For the quantitation of extracellular IL-1β, neutrophils primed with TNF-α or incubated in buffer were stimulated for 8 h with MSU at 37°C, and IL-1β was quantified in the cell-free supernatant using a commercially available ELISA kit obtained from eBioscience (catalog no. 88-7010-22; San Diego, CA, USA). ..

    Article Title: Skin Allografting Activates Anti-tumor Immunity and Suppresses Growth of Colon Cancer in Mice
    Article Snippet: The percentage of each phenotype of immune cells or antibodies was analyzed by using Flowjo software. .. The serum levels of tumor necrosis factor (TNF)-α and interferon (IFN)-γ in BALB/c mice in each group were measured by the ELISA kit ( e Biosciences, San Diego, CA, USA) according to the manufacturer's instructions. .. The optical density (OD) value was measured through the Microplate Reader (Tecan, Männedorf, Switzerland).

    Article Title: Roles of TLR3 and RIG-I in Mediating the Inflammatory Response in Mouse Microglia following Japanese Encephalitis Virus Infection
    Article Snippet: .. Enzyme-Linked Immunosorbent Assay (ELISA) Commercially available ELISA kits (Ebioscience) were used to quantify TNF-α , IL-6, and CCL-2 in the supernatants of BV-2 cells. .. Western Blotting Cells were washed twice with PBS and harvested in RIPA buffer.

    Article Title: Monocytes/Macrophages promote vascular CXCR4 expression via the ERK pathway in hepatocellular carcinoma
    Article Snippet: In some experiments, before stimulating the HUVECs, the conditioned medium was pretreated with neutralizing mAbs against TNF-α or IL-1β (R & D Systems) for 15 min at room temperature. .. The cells were subjected to FACS analysis to detect the surface expression of CXCR4 protein, and the level of IL-6, IL-10, IL-1β, and TNF-α in the conditioned media of the monocytes was determined using commercial ELISA kits, in accordance with the manufacturer's instructions (eBioscience, San Diego, CA). .. To explore the signaling pathways, HUVECs in SFM without ECGS were plated at a density of 4 × 104 cells per well and treated with the Erk1/2 inhibitor, U0126 (Selleck, S1102), or the IκB inhibitor, Bay11-7082 (Selleck, S2913), for 1 hrs followed by the administration of TNF-α (10 ng/mL) for 24 h.

    Article Title: Repeated Low-Dose Influenza Virus Infection Causes Severe Disease in Mice: a Model for Vaccine Evaluation
    Article Snippet: Flow cytometry was performed on a BD LSRII instrument (BD Biosciences, CA, USA), and fluorescence-activated cell sorter (FACS) data were analyzed by using FlowJo software (TreeStar, Ashland, OR, USA). .. Inflammasome responses were determined by detection of interleukin-1β (IL-1β) and IL-18 production in both bronchoalveolar lavage fluid (BALF) and lung homogenates using ELISA kits purchased from eBioscience (San Diego, CA, USA), according to the manufacturer's instructions. ..

    Quantitation Assay:

    Article Title: Modulation of monosodium urate crystal-induced responses in neutrophils by the myeloid inhibitory C-type lectin-like receptor: potential therapeutic implications
    Article Snippet: Extracellular IL-8 was quantified using commercially available ELISA kits (BD Biosciences). .. For the quantitation of extracellular IL-1β, neutrophils primed with TNF-α or incubated in buffer were stimulated for 8 h with MSU at 37°C, and IL-1β was quantified in the cell-free supernatant using a commercially available ELISA kit obtained from eBioscience (catalog no. 88-7010-22; San Diego, CA, USA). ..

    Incubation:

    Article Title: Modulation of monosodium urate crystal-induced responses in neutrophils by the myeloid inhibitory C-type lectin-like receptor: potential therapeutic implications
    Article Snippet: Extracellular IL-8 was quantified using commercially available ELISA kits (BD Biosciences). .. For the quantitation of extracellular IL-1β, neutrophils primed with TNF-α or incubated in buffer were stimulated for 8 h with MSU at 37°C, and IL-1β was quantified in the cell-free supernatant using a commercially available ELISA kit obtained from eBioscience (catalog no. 88-7010-22; San Diego, CA, USA). ..

    Mouse Assay:

    Article Title: Skin Allografting Activates Anti-tumor Immunity and Suppresses Growth of Colon Cancer in Mice
    Article Snippet: The percentage of each phenotype of immune cells or antibodies was analyzed by using Flowjo software. .. The serum levels of tumor necrosis factor (TNF)-α and interferon (IFN)-γ in BALB/c mice in each group were measured by the ELISA kit ( e Biosciences, San Diego, CA, USA) according to the manufacturer's instructions. .. The optical density (OD) value was measured through the Microplate Reader (Tecan, Männedorf, Switzerland).

    FACS:

    Article Title: Monocytes/Macrophages promote vascular CXCR4 expression via the ERK pathway in hepatocellular carcinoma
    Article Snippet: In some experiments, before stimulating the HUVECs, the conditioned medium was pretreated with neutralizing mAbs against TNF-α or IL-1β (R & D Systems) for 15 min at room temperature. .. The cells were subjected to FACS analysis to detect the surface expression of CXCR4 protein, and the level of IL-6, IL-10, IL-1β, and TNF-α in the conditioned media of the monocytes was determined using commercial ELISA kits, in accordance with the manufacturer's instructions (eBioscience, San Diego, CA). .. To explore the signaling pathways, HUVECs in SFM without ECGS were plated at a density of 4 × 104 cells per well and treated with the Erk1/2 inhibitor, U0126 (Selleck, S1102), or the IκB inhibitor, Bay11-7082 (Selleck, S2913), for 1 hrs followed by the administration of TNF-α (10 ng/mL) for 24 h.

    Expressing:

    Article Title: Monocytes/Macrophages promote vascular CXCR4 expression via the ERK pathway in hepatocellular carcinoma
    Article Snippet: In some experiments, before stimulating the HUVECs, the conditioned medium was pretreated with neutralizing mAbs against TNF-α or IL-1β (R & D Systems) for 15 min at room temperature. .. The cells were subjected to FACS analysis to detect the surface expression of CXCR4 protein, and the level of IL-6, IL-10, IL-1β, and TNF-α in the conditioned media of the monocytes was determined using commercial ELISA kits, in accordance with the manufacturer's instructions (eBioscience, San Diego, CA). .. To explore the signaling pathways, HUVECs in SFM without ECGS were plated at a density of 4 × 104 cells per well and treated with the Erk1/2 inhibitor, U0126 (Selleck, S1102), or the IκB inhibitor, Bay11-7082 (Selleck, S2913), for 1 hrs followed by the administration of TNF-α (10 ng/mL) for 24 h.

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  • 99
    Thermo Fisher anti rpp25 elisa nunc immobilizer amino plates
    Correlation between enzyme-linked immunosorbent assay <t>(ELISA)</t> and chemiluminescent immunoassay (CLIA) . The results of 53 samples tested by <t>anti-Rpp25</t> ELISA and CLIA (QUANTA Flash) showed good agreement ( rho = 0.71; P
    Anti Rpp25 Elisa Nunc Immobilizer Amino Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rpp25 elisa nunc immobilizer amino plates/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rpp25 elisa nunc immobilizer amino plates - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher adam17
    Hypercapnia reduces shedding of EGFR ligands in response to stretch via inhibition of <t>ADAM17</t> activity
    Adam17, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adam17/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    adam17 - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    Correlation between enzyme-linked immunosorbent assay (ELISA) and chemiluminescent immunoassay (CLIA) . The results of 53 samples tested by anti-Rpp25 ELISA and CLIA (QUANTA Flash) showed good agreement ( rho = 0.71; P

    Journal: Arthritis Research & Therapy

    Article Title: Rpp25 is a major target of autoantibodies to the Th/To complex as measured by a novel chemiluminescent assay

    doi: 10.1186/ar4210

    Figure Lengend Snippet: Correlation between enzyme-linked immunosorbent assay (ELISA) and chemiluminescent immunoassay (CLIA) . The results of 53 samples tested by anti-Rpp25 ELISA and CLIA (QUANTA Flash) showed good agreement ( rho = 0.71; P

    Article Snippet: Anti-Rpp25 ELISA Nunc Immobilizer Amino plates (Thermo Fisher Scientific, Waltham, MA, USA) were coated with 2 microgram/ml of Rpp25 antigen in PBS, 50 μl/well 2 h at 22°C for 2 h and blocked with 0.5% bovine serum albumin (BSA) in NET/NP40 (150 mM NaCl, 2 mM ethylenediaminetetraacetic acid (EDTA), 50 mM Tris-HCl pH 7.5, 0.3% NP40) for 1 h. Wells were then incubated with sera diluted 1:500 in blocking buffer for 1 h at 22°C.

    Techniques: Enzyme-linked Immunosorbent Assay

    Anti-Rpp25 antibodies in Th/To immunoprecipitation (IP)-positive and IP-negative samples measured by enzyme-linked immunosorbent assay (ELISA) . Comparative descriptive analysis is shown in a). In b) the receiver operating characteristic (ROC) analysis shows good discrimination between anti-Th/To IP-positive patients ( n = 14) and controls (anti-Th/To-negative SSc and healthy individuals, n = 156). AUC, area under the curve.

    Journal: Arthritis Research & Therapy

    Article Title: Rpp25 is a major target of autoantibodies to the Th/To complex as measured by a novel chemiluminescent assay

    doi: 10.1186/ar4210

    Figure Lengend Snippet: Anti-Rpp25 antibodies in Th/To immunoprecipitation (IP)-positive and IP-negative samples measured by enzyme-linked immunosorbent assay (ELISA) . Comparative descriptive analysis is shown in a). In b) the receiver operating characteristic (ROC) analysis shows good discrimination between anti-Th/To IP-positive patients ( n = 14) and controls (anti-Th/To-negative SSc and healthy individuals, n = 156). AUC, area under the curve.

    Article Snippet: Anti-Rpp25 ELISA Nunc Immobilizer Amino plates (Thermo Fisher Scientific, Waltham, MA, USA) were coated with 2 microgram/ml of Rpp25 antigen in PBS, 50 μl/well 2 h at 22°C for 2 h and blocked with 0.5% bovine serum albumin (BSA) in NET/NP40 (150 mM NaCl, 2 mM ethylenediaminetetraacetic acid (EDTA), 50 mM Tris-HCl pH 7.5, 0.3% NP40) for 1 h. Wells were then incubated with sera diluted 1:500 in blocking buffer for 1 h at 22°C.

    Techniques: Immunoprecipitation, Enzyme-linked Immunosorbent Assay

    Hypercapnia reduces shedding of EGFR ligands in response to stretch via inhibition of ADAM17 activity

    Journal: The Journal of Physiology

    Article Title: Hypercapnia attenuates ventilator-induced lung injury via a disintegrin and metalloprotease-17

    doi: 10.1113/jphysiol.2014.277616

    Figure Lengend Snippet: Hypercapnia reduces shedding of EGFR ligands in response to stretch via inhibition of ADAM17 activity

    Article Snippet: Effective blockade of ADAM17 was confirmed in the TAPI-2 group by greater than 10-fold suppression of soluble TNFα in BAL fluid (Fig. C ).

    Techniques: Inhibition, Activity Assay

    Cyclic stretch activation of EGFR and p44/42 MAPK is dependent on ADAM17 activity

    Journal: The Journal of Physiology

    Article Title: Hypercapnia attenuates ventilator-induced lung injury via a disintegrin and metalloprotease-17

    doi: 10.1113/jphysiol.2014.277616

    Figure Lengend Snippet: Cyclic stretch activation of EGFR and p44/42 MAPK is dependent on ADAM17 activity

    Article Snippet: Effective blockade of ADAM17 was confirmed in the TAPI-2 group by greater than 10-fold suppression of soluble TNFα in BAL fluid (Fig. C ).

    Techniques: Activation Assay, Activity Assay

    Inhibition of ADAM17 with TAPI-2 protects mouse lung from LPS/VILI induced injury and decreases p44/42 MAPK activation

    Journal: The Journal of Physiology

    Article Title: Hypercapnia attenuates ventilator-induced lung injury via a disintegrin and metalloprotease-17

    doi: 10.1113/jphysiol.2014.277616

    Figure Lengend Snippet: Inhibition of ADAM17 with TAPI-2 protects mouse lung from LPS/VILI induced injury and decreases p44/42 MAPK activation

    Article Snippet: Effective blockade of ADAM17 was confirmed in the TAPI-2 group by greater than 10-fold suppression of soluble TNFα in BAL fluid (Fig. C ).

    Techniques: Inhibition, Activation Assay