elisa  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    Human respiratory syncytial virus C GFPSpark tagged
    Description:
    Full length Clone DNA of Human RSV subtype A strain RSS 2 Fusion glycoprotein RSV F with C terminal GFPSpark tag
    Catalog Number:
    VG40037-ACG
    Price:
    345.0
    Category:
    cDNA Clone
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Product Aliases:
    F cDNA ORF Clone RSV, HRSVgp08 cDNA ORF Clone RSV
    Molecule Name:
    RSV-F,F,RSV Fusion,
    Buy from Supplier


    Structured Review

    Sino Biological elisa
    Human respiratory syncytial virus C GFPSpark tagged
    Full length Clone DNA of Human RSV subtype A strain RSS 2 Fusion glycoprotein RSV F with C terminal GFPSpark tag
    https://www.bioz.com/result/elisa/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa - by Bioz Stars, 2021-05
    86/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: Antibody-drug conjugate library prepared by scanning insertion of the aldehyde tag into IgG1 constant regions
    Article Snippet: All ADCs were also analyzed by size exclusion chromatography to determine the % aggregate of the final product (Tosoh column 08541; mobile phase: 300 mM Nacl, 25 mM NaPO4, pH 6.8). .. Eleven 1:2 serial dilutions (ranging from 200 to 0.2 ng/mL) of untagged or aldehyde tagged antibodies were captured with HIS-tagged recombinant human antigen (Sino Biological; coated at 1 µg/mL) and detected with a horseradish peroxidase-conjugated goat anti-human IgG Fcg specific secondary antibody (Jackson ImmunoResearch #709–035-098). .. Bound secondary antibody was detected using Ultra TMB One-Step ELISA substrate (Thermo Fisher).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Sino Biological dcr 2
    Cross‐blocking studies with  DCR ‐2 and  UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with  UP ‐D2  PE  at 80 ng·mL −1 . The  MFI  ratio is the comparison between primary antibody and isotype control groups. (A) The change in  UP ‐D2  PE  binding to  CD 300f C CHO  transfectants with different primary antibody staining. (B) Difference in  UP ‐D2 binding to  CD 300f SI 4 ‐ and  CD 300f C ‐transfected  CHO  cells, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . (C) Difference in  UP ‐D2 binding to  AML  cell lines, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . Error bars represent  SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way  ANOVA  with multiple comparisons between groups. *** P
    Dcr 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dcr 2/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dcr 2 - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    85
    Sino Biological human vwf elisa kit
    DIDS inhibits stimulus induced <t>vWF</t> release from normoxic HUVECs. The Ca 2+ ionophore A23187 induces vWF extrusion from HUVECs in a non-pathological model of vesicular release. DIDS abolishes stimulus-evoked vWF release. ( A ) Confocal Z-stack projection fluorescent images of vWF localization (red) in HUVECs treated as indicated. Arrows indicate vWF released extracellularly. ( B ) Summary of supernatant vWF expression measured by <t>ELISA.</t> Data are mean ±SEM from 3 separate 15-min experiments. Asterisks (*) indicate significant difference from normoxic controls; black bars indicate significance between connected treatments ( p
    Human Vwf Elisa Kit, supplied by Sino Biological, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human vwf elisa kit/product/Sino Biological
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human vwf elisa kit - by Bioz Stars, 2021-05
    85/100 stars
      Buy from Supplier

    88
    Sino Biological ceacam6 matched elisa antibody pair set human
    Qualitative Western Blot analysis of bile from patients with benign and malignant disease. The Western Blot analysis utilizes the detection antibody provided in the commercial ELISA kit used in these experiments. The 90kDa <t>CEACAM6</t> is detected between the 95kDa and 72kDa protein ladder bands.
    Ceacam6 Matched Elisa Antibody Pair Set Human, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ceacam6 matched elisa antibody pair set human/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ceacam6 matched elisa antibody pair set human - by Bioz Stars, 2021-05
    88/100 stars
      Buy from Supplier

    93
    Sino Biological hiv 1 infection
    NSC- and A549-derived exosomes significantly enhance <t>HIV-1</t> entry into human immune cell lines. Notes: ( A , C ) YU-2 virus entry into A3R5.7 cells was evaluated in the presence or absence of ( A ) NSC-derived exosomes (0.1 μg) or ( C ) A549-dervied exosomes (0.1 μg). ( B , D ) The differentiated THP2574 cell line was used for entry experiments with YU-2 in the presence or absence of ( B ) NSC-derived exosomes (0.1 μg) or ( D ) A549-derived exosomes (0.1 μg). Virus entry was also evaluated in the presence of exosomes and anti-TIM-4 antibody. Viral gene expression in all control and treatment groups was assessed by Renilla luciferase activity at 72 h post-infection. Data represent 12 independent experiments. Significant differences between treatment groups were determined by one-way ANOVA * P
    Hiv 1 Infection, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiv 1 infection/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiv 1 infection - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Cross‐blocking studies with  DCR ‐2 and  UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with  UP ‐D2  PE  at 80 ng·mL −1 . The  MFI  ratio is the comparison between primary antibody and isotype control groups. (A) The change in  UP ‐D2  PE  binding to  CD 300f C CHO  transfectants with different primary antibody staining. (B) Difference in  UP ‐D2 binding to  CD 300f SI 4 ‐ and  CD 300f C ‐transfected  CHO  cells, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . (C) Difference in  UP ‐D2 binding to  AML  cell lines, which were incubated with a saturating amount of  DCR ‐2 or isotype control prior to  UP ‐D2  PE . Error bars represent  SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way  ANOVA  with multiple comparisons between groups. *** P

    Journal: Molecular Oncology

    Article Title: CD300f epitopes are specific targets for acute myeloid leukemia with monocytic differentiation

    doi: 10.1002/1878-0261.12549

    Figure Lengend Snippet: Cross‐blocking studies with DCR ‐2 and UP ‐D2. Cells were incubated at the saturation point of a primary antibody or an equal concentration of an isotype control and then stained with UP ‐D2 PE at 80 ng·mL −1 . The MFI ratio is the comparison between primary antibody and isotype control groups. (A) The change in UP ‐D2 PE binding to CD 300f C CHO transfectants with different primary antibody staining. (B) Difference in UP ‐D2 binding to CD 300f SI 4 ‐ and CD 300f C ‐transfected CHO cells, which were incubated with a saturating amount of DCR ‐2 or isotype control prior to UP ‐D2 PE . (C) Difference in UP ‐D2 binding to AML cell lines, which were incubated with a saturating amount of DCR ‐2 or isotype control prior to UP ‐D2 PE . Error bars represent SEM . Panel B was analyzed using a t‐test. Panel C was analyzed using a one‐way ANOVA with multiple comparisons between groups. *** P

    Article Snippet: An in‐house mAb, DCR‐2 (IgG1,κ), was generated from a mouse immunized with CD300f Chinese hamster ovary (CHO) transfectants and boosted with recombinant human CD300f‐Fc protein (Sino Biologicals, Beijing, China).

    Techniques: Blocking Assay, Incubation, Concentration Assay, Staining, Binding Assay, Transfection

    Binding of  DCR ‐2 to  CD 300f +  cells enhances the binding of  UP ‐D2 to monocytes, monocytic  AML , but not  CD 34 + HSPC  or nonmonocytic  AML .  CB  or primary frozen  AML  cells were incubated with  PBS , the saturation point of  DCR ‐2 (10 μg·mL −1 ), or an equal concentration of an isotype control. Following primary incubation, samples were stained with  UP ‐D2  PE  at 80 ng·mL −1 . Data for monocytes, lymphocytes, and  CD 34 + HSPC  were obtained from  CB . (A) Difference in  UP ‐D2  PE  binding across cell types when saturated with  DCR ‐2 or isotype control, compared to  PBS . (B) Differences in  UP ‐D2  PE  binding across  CD 34 +  cells between  CB , monocytic  AML , and nonmonocytic  AML . Error bars represent  SEM . Statistical analysis was performed with one‐way  ANOVA  with multiple comparisons between groups. * P

    Journal: Molecular Oncology

    Article Title: CD300f epitopes are specific targets for acute myeloid leukemia with monocytic differentiation

    doi: 10.1002/1878-0261.12549

    Figure Lengend Snippet: Binding of DCR ‐2 to CD 300f + cells enhances the binding of UP ‐D2 to monocytes, monocytic AML , but not CD 34 + HSPC or nonmonocytic AML . CB or primary frozen AML cells were incubated with PBS , the saturation point of DCR ‐2 (10 μg·mL −1 ), or an equal concentration of an isotype control. Following primary incubation, samples were stained with UP ‐D2 PE at 80 ng·mL −1 . Data for monocytes, lymphocytes, and CD 34 + HSPC were obtained from CB . (A) Difference in UP ‐D2 PE binding across cell types when saturated with DCR ‐2 or isotype control, compared to PBS . (B) Differences in UP ‐D2 PE binding across CD 34 + cells between CB , monocytic AML , and nonmonocytic AML . Error bars represent SEM . Statistical analysis was performed with one‐way ANOVA with multiple comparisons between groups. * P

    Article Snippet: An in‐house mAb, DCR‐2 (IgG1,κ), was generated from a mouse immunized with CD300f Chinese hamster ovary (CHO) transfectants and boosted with recombinant human CD300f‐Fc protein (Sino Biologicals, Beijing, China).

    Techniques: Binding Assay, Incubation, Concentration Assay, Staining

    DIDS inhibits stimulus induced vWF release from normoxic HUVECs. The Ca 2+ ionophore A23187 induces vWF extrusion from HUVECs in a non-pathological model of vesicular release. DIDS abolishes stimulus-evoked vWF release. ( A ) Confocal Z-stack projection fluorescent images of vWF localization (red) in HUVECs treated as indicated. Arrows indicate vWF released extracellularly. ( B ) Summary of supernatant vWF expression measured by ELISA. Data are mean ±SEM from 3 separate 15-min experiments. Asterisks (*) indicate significant difference from normoxic controls; black bars indicate significance between connected treatments ( p

    Journal: PLoS ONE

    Article Title: DIDS Prevents Ischemic Membrane Degradation in Cultured Hippocampal Neurons by Inhibiting Matrix Metalloproteinase Release

    doi: 10.1371/journal.pone.0043995

    Figure Lengend Snippet: DIDS inhibits stimulus induced vWF release from normoxic HUVECs. The Ca 2+ ionophore A23187 induces vWF extrusion from HUVECs in a non-pathological model of vesicular release. DIDS abolishes stimulus-evoked vWF release. ( A ) Confocal Z-stack projection fluorescent images of vWF localization (red) in HUVECs treated as indicated. Arrows indicate vWF released extracellularly. ( B ) Summary of supernatant vWF expression measured by ELISA. Data are mean ±SEM from 3 separate 15-min experiments. Asterisks (*) indicate significant difference from normoxic controls; black bars indicate significance between connected treatments ( p

    Article Snippet: ELISA HUVEC vWF release was quantified using a human vWF ELISA kit according to the manufacturer's protocol (Sino Biological Inc., Beijing, CH), using mouse anti-vWF monoclonal antibody and biotinylated rabbit anti-vWF polyclonal antibody as the capture and detection antibodies, respectively.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay

    Qualitative Western Blot analysis of bile from patients with benign and malignant disease. The Western Blot analysis utilizes the detection antibody provided in the commercial ELISA kit used in these experiments. The 90kDa CEACAM6 is detected between the 95kDa and 72kDa protein ladder bands.

    Journal: PLoS ONE

    Article Title: The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma

    doi: 10.1371/journal.pone.0150195

    Figure Lengend Snippet: Qualitative Western Blot analysis of bile from patients with benign and malignant disease. The Western Blot analysis utilizes the detection antibody provided in the commercial ELISA kit used in these experiments. The 90kDa CEACAM6 is detected between the 95kDa and 72kDa protein ladder bands.

    Article Snippet: Bile AnalysisCEACAM6 concentrations were determined by a commercial enzyme-linked immunosorbent assay (SEK10823, Sino Biological, Beijing, China).

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

    Receiver operating characteristic (ROC) curve analysis of CEACAM6 to evaluate presence of cholangiocarcinoma. The ROC analysis of all cholangiocarcinomas is depicted by graph A. with a resultant Area Under the Curve (AUC) of 0.738. Graphs B and C respectively illustrate the intrahepatic and extrahepatic subtypes with corresponding AUCs of 0.663 and 0.791.

    Journal: PLoS ONE

    Article Title: The Role of Biliary Carcinoembryonic Antigen-Related Cellular Adhesion Molecule 6 (CEACAM6) as a Biomarker in Cholangiocarcinoma

    doi: 10.1371/journal.pone.0150195

    Figure Lengend Snippet: Receiver operating characteristic (ROC) curve analysis of CEACAM6 to evaluate presence of cholangiocarcinoma. The ROC analysis of all cholangiocarcinomas is depicted by graph A. with a resultant Area Under the Curve (AUC) of 0.738. Graphs B and C respectively illustrate the intrahepatic and extrahepatic subtypes with corresponding AUCs of 0.663 and 0.791.

    Article Snippet: Bile AnalysisCEACAM6 concentrations were determined by a commercial enzyme-linked immunosorbent assay (SEK10823, Sino Biological, Beijing, China).

    Techniques:

    NSC- and A549-derived exosomes significantly enhance HIV-1 entry into human immune cell lines. Notes: ( A , C ) YU-2 virus entry into A3R5.7 cells was evaluated in the presence or absence of ( A ) NSC-derived exosomes (0.1 μg) or ( C ) A549-dervied exosomes (0.1 μg). ( B , D ) The differentiated THP2574 cell line was used for entry experiments with YU-2 in the presence or absence of ( B ) NSC-derived exosomes (0.1 μg) or ( D ) A549-derived exosomes (0.1 μg). Virus entry was also evaluated in the presence of exosomes and anti-TIM-4 antibody. Viral gene expression in all control and treatment groups was assessed by Renilla luciferase activity at 72 h post-infection. Data represent 12 independent experiments. Significant differences between treatment groups were determined by one-way ANOVA * P

    Journal: International Journal of Nanomedicine

    Article Title: Role of TIM-4 in exosome-dependent entry of HIV-1 into human immune cells

    doi: 10.2147/IJN.S132762

    Figure Lengend Snippet: NSC- and A549-derived exosomes significantly enhance HIV-1 entry into human immune cell lines. Notes: ( A , C ) YU-2 virus entry into A3R5.7 cells was evaluated in the presence or absence of ( A ) NSC-derived exosomes (0.1 μg) or ( C ) A549-dervied exosomes (0.1 μg). ( B , D ) The differentiated THP2574 cell line was used for entry experiments with YU-2 in the presence or absence of ( B ) NSC-derived exosomes (0.1 μg) or ( D ) A549-derived exosomes (0.1 μg). Virus entry was also evaluated in the presence of exosomes and anti-TIM-4 antibody. Viral gene expression in all control and treatment groups was assessed by Renilla luciferase activity at 72 h post-infection. Data represent 12 independent experiments. Significant differences between treatment groups were determined by one-way ANOVA * P

    Article Snippet: Blocking of HIV-1 infection A protocol similar to HIV-1 infection was performed but with addition of 0.2 μg/well anti-mouse TIM-4 or anti-human TIM-4 (Sino Biological, Inc.) to the YU-2/exosome/cell incubation or with YU-2 only as a control.

    Techniques: Derivative Assay, Expressing, Luciferase, Activity Assay, Infection

    Breast milk- and plasma-derived exosomes enhance HIV-1 entry into human immune cell lines. Notes: ( A , C ) YU-2 virus entry into A3R5.7 cells was evaluated in the presence or absence of ( A ) breast milk-derived exosomes (0.035 μg) or ( C ) plasma-derived exosomes (0.05 μg). Virus entry was evaluated in the presence of exosomes and anti-TIM-4 antibodies. ( B , D ) The differentiated THP2574 cell line was used for entry experiments with YU-2 in the presence or absence of ( B ) breast milk-derived exosomes (0.035 μg) or ( D ) plasma-derived exosomes (0.05 μg). Virus entry was also evaluated in the presence of exosomes and anti-TIM-4 antibody. Viral gene expression in all control and treatment groups was assessed by Renilla luciferase activity at 72 h post-infection. Data represent 12 independent experiments. Significant differences between treatment groups were determined by one-way ANOVA. ** P

    Journal: International Journal of Nanomedicine

    Article Title: Role of TIM-4 in exosome-dependent entry of HIV-1 into human immune cells

    doi: 10.2147/IJN.S132762

    Figure Lengend Snippet: Breast milk- and plasma-derived exosomes enhance HIV-1 entry into human immune cell lines. Notes: ( A , C ) YU-2 virus entry into A3R5.7 cells was evaluated in the presence or absence of ( A ) breast milk-derived exosomes (0.035 μg) or ( C ) plasma-derived exosomes (0.05 μg). Virus entry was evaluated in the presence of exosomes and anti-TIM-4 antibodies. ( B , D ) The differentiated THP2574 cell line was used for entry experiments with YU-2 in the presence or absence of ( B ) breast milk-derived exosomes (0.035 μg) or ( D ) plasma-derived exosomes (0.05 μg). Virus entry was also evaluated in the presence of exosomes and anti-TIM-4 antibody. Viral gene expression in all control and treatment groups was assessed by Renilla luciferase activity at 72 h post-infection. Data represent 12 independent experiments. Significant differences between treatment groups were determined by one-way ANOVA. ** P

    Article Snippet: Blocking of HIV-1 infection A protocol similar to HIV-1 infection was performed but with addition of 0.2 μg/well anti-mouse TIM-4 or anti-human TIM-4 (Sino Biological, Inc.) to the YU-2/exosome/cell incubation or with YU-2 only as a control.

    Techniques: Derivative Assay, Expressing, Luciferase, Activity Assay, Infection