il 1β  (Elabscience Biotechnology)

 
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    Elabscience Biotechnology il 1β
    Honokiol suppresses NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. A The molecular formula of honokiol (HKL). B BEAS-2B cells were treated with HKL (0, 6.25, 12.5, 25, 50,100 μM) for 24 or 48 h, and then cytotoxicity was detected by CCK-8 assay. BEAS-2B cells were divided into five groups: control group; LPS + ATP group; LPS + ATP + HKL groups (12.5, 25, 50 μM). BEAS-2B cells in LPS + ATP + HKL groups were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with HKL ( C - D ) using Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×). Nucleus was stained using Hoechst33342. PI staining indicated the loss of plasma membrane integrity. The rate of PI-positive cells was calculated using ImageJ software. E LDH release in supernatant. F , G The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. H The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. I , J The production of IL-18 <t>and</t> <t>IL-1β</t> in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group
    Il 1β, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 1β/product/Elabscience Biotechnology
    Average 86 stars, based on 1 article reviews
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    il 1β - by Bioz Stars, 2023-09
    86/100 stars

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    1) Product Images from "Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo"

    Article Title: Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00541-z

    Honokiol suppresses NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. A The molecular formula of honokiol (HKL). B BEAS-2B cells were treated with HKL (0, 6.25, 12.5, 25, 50,100 μM) for 24 or 48 h, and then cytotoxicity was detected by CCK-8 assay. BEAS-2B cells were divided into five groups: control group; LPS + ATP group; LPS + ATP + HKL groups (12.5, 25, 50 μM). BEAS-2B cells in LPS + ATP + HKL groups were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with HKL ( C - D ) using Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×). Nucleus was stained using Hoechst33342. PI staining indicated the loss of plasma membrane integrity. The rate of PI-positive cells was calculated using ImageJ software. E LDH release in supernatant. F , G The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. H The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. I , J The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group
    Figure Legend Snippet: Honokiol suppresses NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. A The molecular formula of honokiol (HKL). B BEAS-2B cells were treated with HKL (0, 6.25, 12.5, 25, 50,100 μM) for 24 or 48 h, and then cytotoxicity was detected by CCK-8 assay. BEAS-2B cells were divided into five groups: control group; LPS + ATP group; LPS + ATP + HKL groups (12.5, 25, 50 μM). BEAS-2B cells in LPS + ATP + HKL groups were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with HKL ( C - D ) using Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×). Nucleus was stained using Hoechst33342. PI staining indicated the loss of plasma membrane integrity. The rate of PI-positive cells was calculated using ImageJ software. E LDH release in supernatant. F , G The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. H The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. I , J The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group

    Techniques Used: CCK-8 Assay, Staining, Software, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Activation of Nrf2 inhibits NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. BEAS-2B cells were divided into four groups: control group; TBHQ group; LPS + ATP group; LPS + ATP + TBHQ (20 μM) group. BEAS-2B cells in LPS + ATP + TBHQ group were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with TBHQ. A , B Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×) and the rate of PI-positive cell. C , D The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. E LDH release in supernatant. F The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. G , H The production of IL-18 and IL-1β in supernatant were measured by ELISA. I Immunofluorescence staining of GSDMD (original magnification 400 ×). Merged images of DAPI for nucleus (blue) and GSDMD immunofluorescence (green). J The mean fluorescence intensity (MFI) was quantified using ImageJ software. MFI = Integrated Density/Area. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group
    Figure Legend Snippet: Activation of Nrf2 inhibits NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. BEAS-2B cells were divided into four groups: control group; TBHQ group; LPS + ATP group; LPS + ATP + TBHQ (20 μM) group. BEAS-2B cells in LPS + ATP + TBHQ group were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with TBHQ. A , B Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×) and the rate of PI-positive cell. C , D The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. E LDH release in supernatant. F The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. G , H The production of IL-18 and IL-1β in supernatant were measured by ELISA. I Immunofluorescence staining of GSDMD (original magnification 400 ×). Merged images of DAPI for nucleus (blue) and GSDMD immunofluorescence (green). J The mean fluorescence intensity (MFI) was quantified using ImageJ software. MFI = Integrated Density/Area. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group

    Techniques Used: Activation Assay, Staining, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Fluorescence, Software

    Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis through activation of Nrf2 in BEAS-2B cells. A , B Hoechst 33,342 /PI double-fluorescent staining was used to assess the pyroptosis of LPS and ATP-stimulated BEAS-2B cells (original magnification × 100). C The content of LDH in supernatant was measured by an LDH assay kit. D , E The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N and (F) mRNA expression of NLRP3, ASC, CASP1, GSDMD were analyzed by western blot and RT-PCR. G , H The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. ** P < 0.01 compared with the siNC group; # P < 0.05, ## P < 0.01 compared with siNC + LPS + ATP group; & P < 0.05, && P < 0.01 compared with siNrf2 group
    Figure Legend Snippet: Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis through activation of Nrf2 in BEAS-2B cells. A , B Hoechst 33,342 /PI double-fluorescent staining was used to assess the pyroptosis of LPS and ATP-stimulated BEAS-2B cells (original magnification × 100). C The content of LDH in supernatant was measured by an LDH assay kit. D , E The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N and (F) mRNA expression of NLRP3, ASC, CASP1, GSDMD were analyzed by western blot and RT-PCR. G , H The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. ** P < 0.01 compared with the siNC group; # P < 0.05, ## P < 0.01 compared with siNC + LPS + ATP group; & P < 0.05, && P < 0.01 compared with siNrf2 group

    Techniques Used: Activation Assay, Staining, Lactate Dehydrogenase Assay, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis in rats with ALI via activation of Nrf2. A – B , D – E Relative expression levels of NLRP3, ASC, CASP1-P20, GSDMD-N protein in lung tissues were analyzed by western blot. C , F Relative expression levels of NLRP3, ASC, CASP1, GSDMD mRNA in lung tissues were analyzed by RT-PCR. G – I The content of IL-18, IL-1β and LDH in bronchoalveolar lavage fluid of each group were detected by ELISA. J Immunohistochemical staining of GSDMD (original magnification 400 ×) in the rat lungs. K The average optical density (AOD) was used to evaluate the intensity of IHC staining and measured by Image-Pro Plus 6.0 software. Data were analyzed using one-way ANOVA; n = 7–8 rats per group. ** P < 0.01 compared with the control group; # P < 0.05, ## P < 0.01 compared with LPS group
    Figure Legend Snippet: Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis in rats with ALI via activation of Nrf2. A – B , D – E Relative expression levels of NLRP3, ASC, CASP1-P20, GSDMD-N protein in lung tissues were analyzed by western blot. C , F Relative expression levels of NLRP3, ASC, CASP1, GSDMD mRNA in lung tissues were analyzed by RT-PCR. G – I The content of IL-18, IL-1β and LDH in bronchoalveolar lavage fluid of each group were detected by ELISA. J Immunohistochemical staining of GSDMD (original magnification 400 ×) in the rat lungs. K The average optical density (AOD) was used to evaluate the intensity of IHC staining and measured by Image-Pro Plus 6.0 software. Data were analyzed using one-way ANOVA; n = 7–8 rats per group. ** P < 0.01 compared with the control group; # P < 0.05, ## P < 0.01 compared with LPS group

    Techniques Used: Activation Assay, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Immunohistochemistry, Software

    il 18  (Elabscience Biotechnology)

     
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    Elabscience Biotechnology il 18
    Honokiol suppresses NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. A The molecular formula of honokiol (HKL). B BEAS-2B cells were treated with HKL (0, 6.25, 12.5, 25, 50,100 μM) for 24 or 48 h, and then cytotoxicity was detected by CCK-8 assay. BEAS-2B cells were divided into five groups: control group; LPS + ATP group; LPS + ATP + HKL groups (12.5, 25, 50 μM). BEAS-2B cells in LPS + ATP + HKL groups were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with HKL ( C - D ) using Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×). Nucleus was stained using Hoechst33342. PI staining indicated the loss of plasma membrane integrity. The rate of PI-positive cells was calculated using ImageJ software. E LDH release in supernatant. F , G The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. H The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. I , J The production of <t>IL-18</t> and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group
    Il 18, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 18/product/Elabscience Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 18 - by Bioz Stars, 2023-09
    86/100 stars

    Images

    1) Product Images from "Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo"

    Article Title: Honokiol alleviates LPS-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis via Nrf2 activation in vitro and in vivo

    Journal: Chinese Medicine

    doi: 10.1186/s13020-021-00541-z

    Honokiol suppresses NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. A The molecular formula of honokiol (HKL). B BEAS-2B cells were treated with HKL (0, 6.25, 12.5, 25, 50,100 μM) for 24 or 48 h, and then cytotoxicity was detected by CCK-8 assay. BEAS-2B cells were divided into five groups: control group; LPS + ATP group; LPS + ATP + HKL groups (12.5, 25, 50 μM). BEAS-2B cells in LPS + ATP + HKL groups were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with HKL ( C - D ) using Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×). Nucleus was stained using Hoechst33342. PI staining indicated the loss of plasma membrane integrity. The rate of PI-positive cells was calculated using ImageJ software. E LDH release in supernatant. F , G The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. H The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. I , J The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group
    Figure Legend Snippet: Honokiol suppresses NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. A The molecular formula of honokiol (HKL). B BEAS-2B cells were treated with HKL (0, 6.25, 12.5, 25, 50,100 μM) for 24 or 48 h, and then cytotoxicity was detected by CCK-8 assay. BEAS-2B cells were divided into five groups: control group; LPS + ATP group; LPS + ATP + HKL groups (12.5, 25, 50 μM). BEAS-2B cells in LPS + ATP + HKL groups were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with HKL ( C - D ) using Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×). Nucleus was stained using Hoechst33342. PI staining indicated the loss of plasma membrane integrity. The rate of PI-positive cells was calculated using ImageJ software. E LDH release in supernatant. F , G The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. H The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. I , J The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group

    Techniques Used: CCK-8 Assay, Staining, Software, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Activation of Nrf2 inhibits NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. BEAS-2B cells were divided into four groups: control group; TBHQ group; LPS + ATP group; LPS + ATP + TBHQ (20 μM) group. BEAS-2B cells in LPS + ATP + TBHQ group were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with TBHQ. A , B Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×) and the rate of PI-positive cell. C , D The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. E LDH release in supernatant. F The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. G , H The production of IL-18 and IL-1β in supernatant were measured by ELISA. I Immunofluorescence staining of GSDMD (original magnification 400 ×). Merged images of DAPI for nucleus (blue) and GSDMD immunofluorescence (green). J The mean fluorescence intensity (MFI) was quantified using ImageJ software. MFI = Integrated Density/Area. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group
    Figure Legend Snippet: Activation of Nrf2 inhibits NLRP3 inflammasome-mediated pyroptosis in BEAS-2B cells. BEAS-2B cells were divided into four groups: control group; TBHQ group; LPS + ATP group; LPS + ATP + TBHQ (20 μM) group. BEAS-2B cells in LPS + ATP + TBHQ group were stimulated with LPS (1 μg/ml) for 4 h and ATP (5 mM) for 30 min following 20 h pretreatment with TBHQ. A , B Hoechst 33,342 (blue)/PI (red) double-fluorescent staining (100 ×) and the rate of PI-positive cell. C , D The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N were detected by western blot. E LDH release in supernatant. F The mRNA expression of NLRP3, ASC, CASP1, GSDMD were detected by RT-PCR. G , H The production of IL-18 and IL-1β in supernatant were measured by ELISA. I Immunofluorescence staining of GSDMD (original magnification 400 ×). Merged images of DAPI for nucleus (blue) and GSDMD immunofluorescence (green). J The mean fluorescence intensity (MFI) was quantified using ImageJ software. MFI = Integrated Density/Area. Values are expressed as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01 compared with the control group, # P < 0.05, ## P < 0.01 compared with LPS + ATP group

    Techniques Used: Activation Assay, Staining, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Fluorescence, Software

    Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis through activation of Nrf2 in BEAS-2B cells. A , B Hoechst 33,342 /PI double-fluorescent staining was used to assess the pyroptosis of LPS and ATP-stimulated BEAS-2B cells (original magnification × 100). C The content of LDH in supernatant was measured by an LDH assay kit. D , E The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N and (F) mRNA expression of NLRP3, ASC, CASP1, GSDMD were analyzed by western blot and RT-PCR. G , H The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. ** P < 0.01 compared with the siNC group; # P < 0.05, ## P < 0.01 compared with siNC + LPS + ATP group; & P < 0.05, && P < 0.01 compared with siNrf2 group
    Figure Legend Snippet: Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis through activation of Nrf2 in BEAS-2B cells. A , B Hoechst 33,342 /PI double-fluorescent staining was used to assess the pyroptosis of LPS and ATP-stimulated BEAS-2B cells (original magnification × 100). C The content of LDH in supernatant was measured by an LDH assay kit. D , E The protein expression of NLRP3, ASC, CASP1-P20, GSDMD-N and (F) mRNA expression of NLRP3, ASC, CASP1, GSDMD were analyzed by western blot and RT-PCR. G , H The production of IL-18 and IL-1β in supernatant were measured by ELISA. Values are expressed as the mean ± SD of three independent experiments. ** P < 0.01 compared with the siNC group; # P < 0.05, ## P < 0.01 compared with siNC + LPS + ATP group; & P < 0.05, && P < 0.01 compared with siNrf2 group

    Techniques Used: Activation Assay, Staining, Lactate Dehydrogenase Assay, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis in rats with ALI via activation of Nrf2. A – B , D – E Relative expression levels of NLRP3, ASC, CASP1-P20, GSDMD-N protein in lung tissues were analyzed by western blot. C , F Relative expression levels of NLRP3, ASC, CASP1, GSDMD mRNA in lung tissues were analyzed by RT-PCR. G – I The content of IL-18, IL-1β and LDH in bronchoalveolar lavage fluid of each group were detected by ELISA. J Immunohistochemical staining of GSDMD (original magnification 400 ×) in the rat lungs. K The average optical density (AOD) was used to evaluate the intensity of IHC staining and measured by Image-Pro Plus 6.0 software. Data were analyzed using one-way ANOVA; n = 7–8 rats per group. ** P < 0.01 compared with the control group; # P < 0.05, ## P < 0.01 compared with LPS group
    Figure Legend Snippet: Honokiol inhibits NLRP3 inflammasome-mediated pyroptosis in rats with ALI via activation of Nrf2. A – B , D – E Relative expression levels of NLRP3, ASC, CASP1-P20, GSDMD-N protein in lung tissues were analyzed by western blot. C , F Relative expression levels of NLRP3, ASC, CASP1, GSDMD mRNA in lung tissues were analyzed by RT-PCR. G – I The content of IL-18, IL-1β and LDH in bronchoalveolar lavage fluid of each group were detected by ELISA. J Immunohistochemical staining of GSDMD (original magnification 400 ×) in the rat lungs. K The average optical density (AOD) was used to evaluate the intensity of IHC staining and measured by Image-Pro Plus 6.0 software. Data were analyzed using one-way ANOVA; n = 7–8 rats per group. ** P < 0.01 compared with the control group; # P < 0.05, ## P < 0.01 compared with LPS group

    Techniques Used: Activation Assay, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Immunohistochemistry, Software

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