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elisa maxstandard set human gm csf  (Revvity)


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    Structured Review

    Revvity elisa maxstandard set human gm csf
    HPEKs were treated with SGE, AG, GM-CSF, and anti-GM-CSF antibody, incubated for 30 min, and then exposed to UVB irradiation at a dose of 5 mJ/cm 2 . Subsequently, co-culturing with NHEMs was initiated in CnT-PR medium. Forty-eight hours after starting the co-culture, the following experiments were performed. (a) Quantitative PCR (q-PCR) analysis of CSF2 expression in HPEKs. Data are presented as mean ± SEM values from five independent experiments. (b) <t>ELISA</t> analysis of GM-CSF released from HPEKs. Data are presented as mean±SEM values from four independent experiments. (c) Measurement of melanin content in HPEKs, normalized to protein content. Data are represented as mean ± SEM values from four independent experiments. Statistical significance was calculated using one-way ANOVA followed by Dunnett’s test for (a) and (b), and Tukey’s test for (c).
    Elisa Maxstandard Set Human Gm Csf, supplied by Revvity, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa maxstandard set human gm csf/product/Revvity
    Average 86 stars, based on 1 article reviews
    elisa maxstandard set human gm csf - by Bioz Stars, 2025-07
    86/100 stars

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    1) Product Images from "Sage extract and ascorbic acid derivative inhibit melanogenesis via downregulating keratinocyte-derived GM-CSF"

    Article Title: Sage extract and ascorbic acid derivative inhibit melanogenesis via downregulating keratinocyte-derived GM-CSF

    Journal: PLOS One

    doi: 10.1371/journal.pone.0325242

    HPEKs were treated with SGE, AG, GM-CSF, and anti-GM-CSF antibody, incubated for 30 min, and then exposed to UVB irradiation at a dose of 5 mJ/cm 2 . Subsequently, co-culturing with NHEMs was initiated in CnT-PR medium. Forty-eight hours after starting the co-culture, the following experiments were performed. (a) Quantitative PCR (q-PCR) analysis of CSF2 expression in HPEKs. Data are presented as mean ± SEM values from five independent experiments. (b) ELISA analysis of GM-CSF released from HPEKs. Data are presented as mean±SEM values from four independent experiments. (c) Measurement of melanin content in HPEKs, normalized to protein content. Data are represented as mean ± SEM values from four independent experiments. Statistical significance was calculated using one-way ANOVA followed by Dunnett’s test for (a) and (b), and Tukey’s test for (c).
    Figure Legend Snippet: HPEKs were treated with SGE, AG, GM-CSF, and anti-GM-CSF antibody, incubated for 30 min, and then exposed to UVB irradiation at a dose of 5 mJ/cm 2 . Subsequently, co-culturing with NHEMs was initiated in CnT-PR medium. Forty-eight hours after starting the co-culture, the following experiments were performed. (a) Quantitative PCR (q-PCR) analysis of CSF2 expression in HPEKs. Data are presented as mean ± SEM values from five independent experiments. (b) ELISA analysis of GM-CSF released from HPEKs. Data are presented as mean±SEM values from four independent experiments. (c) Measurement of melanin content in HPEKs, normalized to protein content. Data are represented as mean ± SEM values from four independent experiments. Statistical significance was calculated using one-way ANOVA followed by Dunnett’s test for (a) and (b), and Tukey’s test for (c).

    Techniques Used: Incubation, Irradiation, Co-Culture Assay, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay



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    Revvity elisa maxstandard set human gm csf
    HPEKs were treated with SGE, AG, GM-CSF, and anti-GM-CSF antibody, incubated for 30 min, and then exposed to UVB irradiation at a dose of 5 mJ/cm 2 . Subsequently, co-culturing with NHEMs was initiated in CnT-PR medium. Forty-eight hours after starting the co-culture, the following experiments were performed. (a) Quantitative PCR (q-PCR) analysis of CSF2 expression in HPEKs. Data are presented as mean ± SEM values from five independent experiments. (b) <t>ELISA</t> analysis of GM-CSF released from HPEKs. Data are presented as mean±SEM values from four independent experiments. (c) Measurement of melanin content in HPEKs, normalized to protein content. Data are represented as mean ± SEM values from four independent experiments. Statistical significance was calculated using one-way ANOVA followed by Dunnett’s test for (a) and (b), and Tukey’s test for (c).
    Elisa Maxstandard Set Human Gm Csf, supplied by Revvity, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa maxstandard set human gm csf/product/Revvity
    Average 86 stars, based on 1 article reviews
    elisa maxstandard set human gm csf - by Bioz Stars, 2025-07
    86/100 stars
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    HPEKs were treated with SGE, AG, GM-CSF, and anti-GM-CSF antibody, incubated for 30 min, and then exposed to UVB irradiation at a dose of 5 mJ/cm 2 . Subsequently, co-culturing with NHEMs was initiated in CnT-PR medium. Forty-eight hours after starting the co-culture, the following experiments were performed. (a) Quantitative PCR (q-PCR) analysis of CSF2 expression in HPEKs. Data are presented as mean ± SEM values from five independent experiments. (b) ELISA analysis of GM-CSF released from HPEKs. Data are presented as mean±SEM values from four independent experiments. (c) Measurement of melanin content in HPEKs, normalized to protein content. Data are represented as mean ± SEM values from four independent experiments. Statistical significance was calculated using one-way ANOVA followed by Dunnett’s test for (a) and (b), and Tukey’s test for (c).

    Journal: PLOS One

    Article Title: Sage extract and ascorbic acid derivative inhibit melanogenesis via downregulating keratinocyte-derived GM-CSF

    doi: 10.1371/journal.pone.0325242

    Figure Lengend Snippet: HPEKs were treated with SGE, AG, GM-CSF, and anti-GM-CSF antibody, incubated for 30 min, and then exposed to UVB irradiation at a dose of 5 mJ/cm 2 . Subsequently, co-culturing with NHEMs was initiated in CnT-PR medium. Forty-eight hours after starting the co-culture, the following experiments were performed. (a) Quantitative PCR (q-PCR) analysis of CSF2 expression in HPEKs. Data are presented as mean ± SEM values from five independent experiments. (b) ELISA analysis of GM-CSF released from HPEKs. Data are presented as mean±SEM values from four independent experiments. (c) Measurement of melanin content in HPEKs, normalized to protein content. Data are represented as mean ± SEM values from four independent experiments. Statistical significance was calculated using one-way ANOVA followed by Dunnett’s test for (a) and (b), and Tukey’s test for (c).

    Article Snippet: The levels of human Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in the cell culture supernatants of HPEKs were measured using the ELISA MAXStandard Set Human GM-CSF (BIOLEGEND, San Diego, CA, USA) according to the manufacturer’s instructions.

    Techniques: Incubation, Irradiation, Co-Culture Assay, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay