elisa kits  (Thermo Fisher)


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    Name:
    Ethylene Glycol
    Description:
    Thermo Scientific Pierce Ethylene Glycol Solution provides exceptional antifreeze protection and storage stability for antibody enzyme conjugates because it is purified to remove impurities commonly found traditional glycerol stocks Features of Ethylene Glycol • Specially purified to remove impurities such as aldehydes peroxides iron and UV absorbing hydrocarbons • Suitable for enzyme storage without the worry of losing enzymatic activity • Stable for months This product is a 50 w v aqueous solution of highly purified ethylene glycol When mixed in equal volume with purified protein samples such as primary antibodies the solution stabilizes and maintains the mixture as a liquid during freezer storage 20° Ethylene glycol is a suitable alternative to glycerol for most protein storage applications In fact this preparation of ethylene glycol is devoid of various common glycerol impurities and potiential degradation products that are damaging to certain enzymes
    Catalog Number:
    29810
    Price:
    None
    Category:
    Lab Reagents and Chemicals
    Applications:
    Antibody Labeling|Antibody Production and Labeling|Build Your Own Immunoassay|Cell Analysis|ELISA|Protein Assays and Analysis|Protein Biology|Western Blotting
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    Structured Review

    Thermo Fisher elisa kits
    Cytokine production by HSPCs in response to C. albicans and the TLR2 agonist. (A) Schematic protocol (as described in section Materials and Methods) to obtain <t>secretomes.</t> WT Lin − cells were cultured at a density of 50,000 cells in 250 μl of a serum-free medium containing FL and SCF, in the presence or absence of Pam 3 CSK 4 or inactivated C. albicans yeasts for 3 days. Then, secretomes (conditioned media) were collected. (B) TNF-α and IL-6 levels in the secretomes were measured by <t>ELISA.</t> (C) CCL2, CCL3, and CCL9 levels were determined using a mouse cytokine array (as described in section Materials and Methods). Data are expressed as means ± SD of pooled data from two experiments. ** P
    Thermo Scientific Pierce Ethylene Glycol Solution provides exceptional antifreeze protection and storage stability for antibody enzyme conjugates because it is purified to remove impurities commonly found traditional glycerol stocks Features of Ethylene Glycol • Specially purified to remove impurities such as aldehydes peroxides iron and UV absorbing hydrocarbons • Suitable for enzyme storage without the worry of losing enzymatic activity • Stable for months This product is a 50 w v aqueous solution of highly purified ethylene glycol When mixed in equal volume with purified protein samples such as primary antibodies the solution stabilizes and maintains the mixture as a liquid during freezer storage 20° Ethylene glycol is a suitable alternative to glycerol for most protein storage applications In fact this preparation of ethylene glycol is devoid of various common glycerol impurities and potiential degradation products that are damaging to certain enzymes
    https://www.bioz.com/result/elisa kits/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kits - by Bioz Stars, 2021-03
    99/100 stars

    Images

    1) Product Images from "Systemic Candidiasis and TLR2 Agonist Exposure Impact the Antifungal Response of Hematopoietic Stem and Progenitor Cells"

    Article Title: Systemic Candidiasis and TLR2 Agonist Exposure Impact the Antifungal Response of Hematopoietic Stem and Progenitor Cells

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2018.00309

    Cytokine production by HSPCs in response to C. albicans and the TLR2 agonist. (A) Schematic protocol (as described in section Materials and Methods) to obtain secretomes. WT Lin − cells were cultured at a density of 50,000 cells in 250 μl of a serum-free medium containing FL and SCF, in the presence or absence of Pam 3 CSK 4 or inactivated C. albicans yeasts for 3 days. Then, secretomes (conditioned media) were collected. (B) TNF-α and IL-6 levels in the secretomes were measured by ELISA. (C) CCL2, CCL3, and CCL9 levels were determined using a mouse cytokine array (as described in section Materials and Methods). Data are expressed as means ± SD of pooled data from two experiments. ** P
    Figure Legend Snippet: Cytokine production by HSPCs in response to C. albicans and the TLR2 agonist. (A) Schematic protocol (as described in section Materials and Methods) to obtain secretomes. WT Lin − cells were cultured at a density of 50,000 cells in 250 μl of a serum-free medium containing FL and SCF, in the presence or absence of Pam 3 CSK 4 or inactivated C. albicans yeasts for 3 days. Then, secretomes (conditioned media) were collected. (B) TNF-α and IL-6 levels in the secretomes were measured by ELISA. (C) CCL2, CCL3, and CCL9 levels were determined using a mouse cytokine array (as described in section Materials and Methods). Data are expressed as means ± SD of pooled data from two experiments. ** P

    Techniques Used: Cell Culture, Enzyme-linked Immunosorbent Assay

    C. albicans infection impacts the cytokine production and the fungicidal activity of the ex vivo produced macrophages. (A) Schematic protocol (as described in section Materials and Methods). WT mice were injected intraperitoneally with 45 × 10 6 yeasts of C. albicans ATCC 26555. 1 or 3 days post-infection mice were sacrificed to assess the outgrowth of the yeasts in the kidney, and to isolate the bone marrow. Lin − HSPCs were purified from bone marrow, plated at a density of 200,000 cells in 4 ml of culture medium containing SCF, M-CSF and amphotericin B, and incubated for 7 days to induce macrophage differentiation. (B) The fungal burden in the kidneys is expressed as CFUs per gram of tissue. For cytokine assays, macrophages were plated at a density of 50,000 cells in 200 μl of complete cell culture medium and challenged with Pam 3 CSK 4 (100 ng/ml) or LPS (100 ng/ml) for 24 h. TNF-α levels in cell-free culture supernatants were measured by ELISA. For fungicidal activity determination, macrophages were plated at a density of 200,000 cells in 200 μl of complete cell culture medium and challenged with viable PCA2 yeasts at a 1:3 ratio (murine cell:yeast) for 1 h. C. albicans cells were also inoculated in culture medium without murine cells (control). After incubation, samples were diluted, plated on Sabouraud dextrose agar and incubated overnight at 37°C; CFUs were counted and killing percentages were determined as follows: % killing = [1 – (CFUs sample at t = 1 h)/(CFUs control at t = 1 h)] × 100. Triplicate samples were analyzed in each assay. Results are expressed as means ± SD of pooled data from two experiments. * P
    Figure Legend Snippet: C. albicans infection impacts the cytokine production and the fungicidal activity of the ex vivo produced macrophages. (A) Schematic protocol (as described in section Materials and Methods). WT mice were injected intraperitoneally with 45 × 10 6 yeasts of C. albicans ATCC 26555. 1 or 3 days post-infection mice were sacrificed to assess the outgrowth of the yeasts in the kidney, and to isolate the bone marrow. Lin − HSPCs were purified from bone marrow, plated at a density of 200,000 cells in 4 ml of culture medium containing SCF, M-CSF and amphotericin B, and incubated for 7 days to induce macrophage differentiation. (B) The fungal burden in the kidneys is expressed as CFUs per gram of tissue. For cytokine assays, macrophages were plated at a density of 50,000 cells in 200 μl of complete cell culture medium and challenged with Pam 3 CSK 4 (100 ng/ml) or LPS (100 ng/ml) for 24 h. TNF-α levels in cell-free culture supernatants were measured by ELISA. For fungicidal activity determination, macrophages were plated at a density of 200,000 cells in 200 μl of complete cell culture medium and challenged with viable PCA2 yeasts at a 1:3 ratio (murine cell:yeast) for 1 h. C. albicans cells were also inoculated in culture medium without murine cells (control). After incubation, samples were diluted, plated on Sabouraud dextrose agar and incubated overnight at 37°C; CFUs were counted and killing percentages were determined as follows: % killing = [1 – (CFUs sample at t = 1 h)/(CFUs control at t = 1 h)] × 100. Triplicate samples were analyzed in each assay. Results are expressed as means ± SD of pooled data from two experiments. * P

    Techniques Used: Infection, Activity Assay, Ex Vivo, Produced, Mouse Assay, Injection, Purification, Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Glycyrrhizin Attenuates Salmonella enterica Serovar Typhimurium Infection: New Insights Into Its Protective Mechanism"

    Article Title: Glycyrrhizin Attenuates Salmonella enterica Serovar Typhimurium Infection: New Insights Into Its Protective Mechanism

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.02321

    Effect of GL on serum cytokine secretion in normal and ST-infected mice. The mice were infected orally with GL, ST, GL plus ST (GL prevention group), or GM plus ST (GM prevention group). After 72 h of infection, body weight was monitored every 3 days over 21 days. PBS served as a negative control. IFN-γ (A) , IL-12p70 (B) , TNF-α (C) , IL-6 (D) and IL-10 (E) secretion in serum was quantified by ELISA, and the data are expressed as the mean ± SD ( n = 5/group; ns, not significant; * P
    Figure Legend Snippet: Effect of GL on serum cytokine secretion in normal and ST-infected mice. The mice were infected orally with GL, ST, GL plus ST (GL prevention group), or GM plus ST (GM prevention group). After 72 h of infection, body weight was monitored every 3 days over 21 days. PBS served as a negative control. IFN-γ (A) , IL-12p70 (B) , TNF-α (C) , IL-6 (D) and IL-10 (E) secretion in serum was quantified by ELISA, and the data are expressed as the mean ± SD ( n = 5/group; ns, not significant; * P

    Techniques Used: Infection, Mouse Assay, Negative Control, Enzyme-linked Immunosorbent Assay

    Effect of GL on colonic cytokine secretion in normal and ST-infected mice. The mice were infected orally with GL, ST, GL plus ST (glycyrrhizin prevention group), or GM plus ST (gentamicin prevention group). After 72 h of infection, body weight was monitored every 3 days over 21 days. PBS served as a negative control. NO (A) , IFN-γ (B) , IL-12p70 (C) , TNF-α (D) , IL-6 (E) and IL-10 (F) secretion in colon were quantified by ELISA, and the data are expressed as the mean ± SD ( n = 5/group; ns, not significant; * P
    Figure Legend Snippet: Effect of GL on colonic cytokine secretion in normal and ST-infected mice. The mice were infected orally with GL, ST, GL plus ST (glycyrrhizin prevention group), or GM plus ST (gentamicin prevention group). After 72 h of infection, body weight was monitored every 3 days over 21 days. PBS served as a negative control. NO (A) , IFN-γ (B) , IL-12p70 (C) , TNF-α (D) , IL-6 (E) and IL-10 (F) secretion in colon were quantified by ELISA, and the data are expressed as the mean ± SD ( n = 5/group; ns, not significant; * P

    Techniques Used: Infection, Mouse Assay, Negative Control, Enzyme-linked Immunosorbent Assay

    Effect of GL on ileac cytokine secretion in normal and ST-infected mice. The mice were infected orally with GL, ST, GL plus ST (glycyrrhizin prevention group) and GM plus ST (gentamicin prevention group). Body weight was monitored every 3 days over 21 days. PBS served as a negative control. After 72 h of infection, IFN-γ (A) , IL-12p70 (B) , TNF-α (C) , IL-6 (D) and IL-10 (E) secretion in the ileum were quantified by ELISA, and the data are expressed as the mean ± SD ( n = 5/group; ns, not significant; * P
    Figure Legend Snippet: Effect of GL on ileac cytokine secretion in normal and ST-infected mice. The mice were infected orally with GL, ST, GL plus ST (glycyrrhizin prevention group) and GM plus ST (gentamicin prevention group). Body weight was monitored every 3 days over 21 days. PBS served as a negative control. After 72 h of infection, IFN-γ (A) , IL-12p70 (B) , TNF-α (C) , IL-6 (D) and IL-10 (E) secretion in the ileum were quantified by ELISA, and the data are expressed as the mean ± SD ( n = 5/group; ns, not significant; * P

    Techniques Used: Infection, Mouse Assay, Negative Control, Enzyme-linked Immunosorbent Assay

    3) Product Images from "Downregulated expression of microRNA-124 in pediatric intestinal failure patients modulates macrophages activation by inhibiting STAT3 and AChE"

    Article Title: Downregulated expression of microRNA-124 in pediatric intestinal failure patients modulates macrophages activation by inhibiting STAT3 and AChE

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2016.426

    miR-124 attenuates the IL-6 and TNF- α production in macrophages. ELISA assay showed that overexpression of miR-124 significantly suppressed the LPS-induced IL-6 and TNF- α production in macrophages. ( a and b ) The human macrophages derived from THP-1 cells. ( c and d ) The mice macrophages RAW264.7. The static, Ach and itopride inhibited the IL-6 and TNF- α production in macrophages. ( e and f ) The human macrophages induced from THP-1 cells. ( g and h ) The RAW264.7 cells. ( i - l ) The expression of p-STAT3 and AChE proteins. * P
    Figure Legend Snippet: miR-124 attenuates the IL-6 and TNF- α production in macrophages. ELISA assay showed that overexpression of miR-124 significantly suppressed the LPS-induced IL-6 and TNF- α production in macrophages. ( a and b ) The human macrophages derived from THP-1 cells. ( c and d ) The mice macrophages RAW264.7. The static, Ach and itopride inhibited the IL-6 and TNF- α production in macrophages. ( e and f ) The human macrophages induced from THP-1 cells. ( g and h ) The RAW264.7 cells. ( i - l ) The expression of p-STAT3 and AChE proteins. * P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Over Expression, Derivative Assay, Mouse Assay, Expressing

    4) Product Images from "The role of synovial macrophages and macrophage-produced cytokines in driving aggrecanases, matrix metalloproteinases, and other destructive and inflammatory responses in osteoarthritis"

    Article Title: The role of synovial macrophages and macrophage-produced cytokines in driving aggrecanases, matrix metalloproteinases, and other destructive and inflammatory responses in osteoarthritis

    Journal: Arthritis Research & Therapy

    doi: 10.1186/ar2099

    Effect of macrophage depletion on cytokine and matrix metalloproteinase (MMP) production in osteoarthritis (OA) synovial cells. OA cultures of synovial cells were either left intact or macrophage-depleted, as described in Materials and methods. Cells were left to adhere for 24 hours before the supernatants were removed for enzyme-linked immunosorbent assay analysis of cytokines and MMPs. Data are expressed as the percentage of cytokine/MMP production in the depleted culture as compared with the undepleted one. The standard error of the mean is given ( n = 7–9). IL, interleukin; MCP, monocyte chemoattractant protein; TNF, tumour necrosis factor.
    Figure Legend Snippet: Effect of macrophage depletion on cytokine and matrix metalloproteinase (MMP) production in osteoarthritis (OA) synovial cells. OA cultures of synovial cells were either left intact or macrophage-depleted, as described in Materials and methods. Cells were left to adhere for 24 hours before the supernatants were removed for enzyme-linked immunosorbent assay analysis of cytokines and MMPs. Data are expressed as the percentage of cytokine/MMP production in the depleted culture as compared with the undepleted one. The standard error of the mean is given ( n = 7–9). IL, interleukin; MCP, monocyte chemoattractant protein; TNF, tumour necrosis factor.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Effect of neutralisation of tumour necrosis factor (TNF)-α and/or interleukin (IL)-1 on cytokine and matrix metalloproteinase (MMP) production in osteoarthritis synovial cells. In these experiments, 2 × 10 6 cells per well were plated into 4 wells on a 24-well plate in 1 ml of RPMI 1640 supplemented with 10% foetal calf serum. The cells in these 4 wells were left untreated, incubated with the p75 TNF-soluble receptor-immunoglobulin fusion protein etanercept (Enbrel), incubated with a neutralising anti-IL-1β antibody, or incubated with a combination of etanercept and anti-IL-1β, as described in Materials and methods. After incubation for 48 hours, the supernatants were removed for enzyme-linked immunosorbent assay analysis of various cytokines (a) and MMPs (b) . The data are expressed as percentage of the production of untreated cells, and the standard error of the mean is given ( n = 6–7).
    Figure Legend Snippet: Effect of neutralisation of tumour necrosis factor (TNF)-α and/or interleukin (IL)-1 on cytokine and matrix metalloproteinase (MMP) production in osteoarthritis synovial cells. In these experiments, 2 × 10 6 cells per well were plated into 4 wells on a 24-well plate in 1 ml of RPMI 1640 supplemented with 10% foetal calf serum. The cells in these 4 wells were left untreated, incubated with the p75 TNF-soluble receptor-immunoglobulin fusion protein etanercept (Enbrel), incubated with a neutralising anti-IL-1β antibody, or incubated with a combination of etanercept and anti-IL-1β, as described in Materials and methods. After incubation for 48 hours, the supernatants were removed for enzyme-linked immunosorbent assay analysis of various cytokines (a) and MMPs (b) . The data are expressed as percentage of the production of untreated cells, and the standard error of the mean is given ( n = 6–7).

    Techniques Used: Incubation, Enzyme-linked Immunosorbent Assay

    5) Product Images from "MAP1981c, a Putative Nucleic Acid-Binding Protein, Produced by Mycobacterium avium subsp. paratuberculosis, Induces Maturation of Dendritic Cells and Th1-Polarization"

    Article Title: MAP1981c, a Putative Nucleic Acid-Binding Protein, Produced by Mycobacterium avium subsp. paratuberculosis, Induces Maturation of Dendritic Cells and Th1-Polarization

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2018.00206

    Cytokine production by MAP1981c stimulation in DCs. (A) The DCs were stimulated with MAP1981c (1, 2, or 5 μg/mL), LPS (100 ng/mL), or PBS (Con) for 24 h and the culture supernatants were analyzed for the amounts of TNF-α, IL-6, IL-1β, L-12p70, and IL-10 by using quantitative ELISA. (B) Dot plots of intracellular IL-12p70 and IL-10 in CD11c + DCs. DCs were stimulated with MAP1981c (1, 2, or 5 μg/mL), LPS (100 ng/mL) for 12 h in the presence of GolgiPlug. The intracellular IL-12p70 and IL-10 levels were analyzed in CD11c + DCs by flow cytometry. All bar graphs show the means ± SD of 3 samples. One representative plot out of three independent experiments is shown; * p
    Figure Legend Snippet: Cytokine production by MAP1981c stimulation in DCs. (A) The DCs were stimulated with MAP1981c (1, 2, or 5 μg/mL), LPS (100 ng/mL), or PBS (Con) for 24 h and the culture supernatants were analyzed for the amounts of TNF-α, IL-6, IL-1β, L-12p70, and IL-10 by using quantitative ELISA. (B) Dot plots of intracellular IL-12p70 and IL-10 in CD11c + DCs. DCs were stimulated with MAP1981c (1, 2, or 5 μg/mL), LPS (100 ng/mL) for 12 h in the presence of GolgiPlug. The intracellular IL-12p70 and IL-10 levels were analyzed in CD11c + DCs by flow cytometry. All bar graphs show the means ± SD of 3 samples. One representative plot out of three independent experiments is shown; * p

    Techniques Used: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Cytometry

    Confirmation of no LPS contamination of the recombinant MAP1981c. (A) DCs were treated with MAP1981c (5 μg/mL) and LPS (100 ng/mL) in presence and absence of PMB. (B) DCs were treated with MAP1981c, LPS, boiled MAP1981c, and boiled LPS. (C) DCs were treated with MAP1981c and LPS in presence and absence of PK. After 24 h treatment, the culture supernatants were analyzed for the amounts of TNF-α, IL-6, and IL-1β by using ELISA. All bar graphs show the means ± SD of 3 samples. One representative plot out of three independent experiments is shown. The value of n. s. was defined as having no significant effect. The value of *** p
    Figure Legend Snippet: Confirmation of no LPS contamination of the recombinant MAP1981c. (A) DCs were treated with MAP1981c (5 μg/mL) and LPS (100 ng/mL) in presence and absence of PMB. (B) DCs were treated with MAP1981c, LPS, boiled MAP1981c, and boiled LPS. (C) DCs were treated with MAP1981c and LPS in presence and absence of PK. After 24 h treatment, the culture supernatants were analyzed for the amounts of TNF-α, IL-6, and IL-1β by using ELISA. All bar graphs show the means ± SD of 3 samples. One representative plot out of three independent experiments is shown. The value of n. s. was defined as having no significant effect. The value of *** p

    Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay

    MAP1981c-induced DC maturation via TLR4 signaling. (A,B) WT-, TLR2 K/O, and TLR4 K/O-derived DCs were stimulated with MAP1981c (5 μg/mL) and LPS (100 ng/mL) for 24 h and analyzed for the expression of surface molecules (CD86 and MHC-II) and the production of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) by flow cytometry (A) and ELISA (B) , respectively. (C) WT-, MyD88 K/O, and TRIF K/O-derived DCs were stimulated with MAP1981c (5 μg/mL) and LPS (100 ng/mL) for 24 h and analyzed for the production of TNF-α, IL-6, and IL-1β by ELISA. All bar graphs show the means ± SD of 3 samples. One representative plot out of three independent experiments is shown; *** p
    Figure Legend Snippet: MAP1981c-induced DC maturation via TLR4 signaling. (A,B) WT-, TLR2 K/O, and TLR4 K/O-derived DCs were stimulated with MAP1981c (5 μg/mL) and LPS (100 ng/mL) for 24 h and analyzed for the expression of surface molecules (CD86 and MHC-II) and the production of pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β) by flow cytometry (A) and ELISA (B) , respectively. (C) WT-, MyD88 K/O, and TRIF K/O-derived DCs were stimulated with MAP1981c (5 μg/mL) and LPS (100 ng/mL) for 24 h and analyzed for the production of TNF-α, IL-6, and IL-1β by ELISA. All bar graphs show the means ± SD of 3 samples. One representative plot out of three independent experiments is shown; *** p

    Techniques Used: Derivative Assay, Expressing, Flow Cytometry, Cytometry, Enzyme-linked Immunosorbent Assay

    6) Product Images from "The host response to smallpox: Analysis of the gene expression program in peripheral blood cells in a nonhuman primate model"

    Article Title: The host response to smallpox: Analysis of the gene expression program in peripheral blood cells in a nonhuman primate model

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.0405759101

    IFN response. ( A ) Gene cluster associated with the IFN response. Colored bars indicate rows of data from two or more independent array elements representing the same gene. ( B and C ) IFN-γ ( B ) and IFN-α ( C ) production, as measured by ELISA
    Figure Legend Snippet: IFN response. ( A ) Gene cluster associated with the IFN response. Colored bars indicate rows of data from two or more independent array elements representing the same gene. ( B and C ) IFN-γ ( B ) and IFN-α ( C ) production, as measured by ELISA

    Techniques Used: Enzyme-linked Immunosorbent Assay

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    BAC Assay:

    Article Title: Poleward transport of Eg5 by dynein-dynactin in Xenopus laevis egg extract spindles
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    Expressing:

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    Isolation:

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    Enzyme-linked Immunosorbent Assay:

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    other:

    Article Title: Crowder-Induced Conformational Ensemble Shift in Escherichia coli Prolyl-tRNA Synthetase
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    Article Title: Cross-linked Polystyrene Sulfonic Acid and Polyethylene Glycol as a Low-fouling Material
    Article Snippet: 75,000 g/mol Polystyrene sulfonic acid (PSS) was purchased from Sigma; 20,000 g/mol Polyethylene glycol (PEG) was purchased from Alfa Aesar.

    Incubation:

    Article Title: Allele-specific siRNA silencing for the common Keratin 12 founder mutation in Meesmann epithelial corneal dystrophy
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    Transfection:

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    Plasmid Preparation:

    Article Title: Allele-specific siRNA silencing for the common Keratin 12 founder mutation in Meesmann epithelial corneal dystrophy
    Article Snippet: All PCR reactions were carried out using GoTaq Flexi DNA polymerase system (Promega, Southampton, UK) according to manufacturer’s instructions using the following cycling conditions: 94°C for 4 min, then 32 cycles of 95°C for 30s, 62°C for 30s and 72°C for 15s, followed by 5 min at 72°C and a 4°C hold. .. 3.5 × 105 AD293 cells were seeded in 2ml DMEM (10% FBS) in a 6 well plate, incubated for 24h and transfected with 1μg of plasmid DNA expressing K12-Arg135Thr-eGFP fusion protein using 3.3μl Lipofectamine 2000 (Invitrogen, Paisley, UK) in Optimem serum-free medium following manufacturer’s instructions. .. After 24h the medium was replaced and the cells transfected with siRNA K12-Arg135Thr-5 to a final concentration of 3nM using 6μl of Lipofectamine RNAiMAX (Invitrogen, Paisley, UK) according to the manufacturer’s instructions for 6h.

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    Thermo Fisher adam17
    Hypercapnia reduces shedding of EGFR ligands in response to stretch via inhibition of <t>ADAM17</t> activity
    Adam17, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    adam17 - by Bioz Stars, 2021-03
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    Hypercapnia reduces shedding of EGFR ligands in response to stretch via inhibition of ADAM17 activity

    Journal: The Journal of Physiology

    Article Title: Hypercapnia attenuates ventilator-induced lung injury via a disintegrin and metalloprotease-17

    doi: 10.1113/jphysiol.2014.277616

    Figure Lengend Snippet: Hypercapnia reduces shedding of EGFR ligands in response to stretch via inhibition of ADAM17 activity

    Article Snippet: Effective blockade of ADAM17 was confirmed in the TAPI-2 group by greater than 10-fold suppression of soluble TNFα in BAL fluid (Fig. C ).

    Techniques: Inhibition, Activity Assay

    Cyclic stretch activation of EGFR and p44/42 MAPK is dependent on ADAM17 activity

    Journal: The Journal of Physiology

    Article Title: Hypercapnia attenuates ventilator-induced lung injury via a disintegrin and metalloprotease-17

    doi: 10.1113/jphysiol.2014.277616

    Figure Lengend Snippet: Cyclic stretch activation of EGFR and p44/42 MAPK is dependent on ADAM17 activity

    Article Snippet: Effective blockade of ADAM17 was confirmed in the TAPI-2 group by greater than 10-fold suppression of soluble TNFα in BAL fluid (Fig. C ).

    Techniques: Activation Assay, Activity Assay

    Inhibition of ADAM17 with TAPI-2 protects mouse lung from LPS/VILI induced injury and decreases p44/42 MAPK activation

    Journal: The Journal of Physiology

    Article Title: Hypercapnia attenuates ventilator-induced lung injury via a disintegrin and metalloprotease-17

    doi: 10.1113/jphysiol.2014.277616

    Figure Lengend Snippet: Inhibition of ADAM17 with TAPI-2 protects mouse lung from LPS/VILI induced injury and decreases p44/42 MAPK activation

    Article Snippet: Effective blockade of ADAM17 was confirmed in the TAPI-2 group by greater than 10-fold suppression of soluble TNFα in BAL fluid (Fig. C ).

    Techniques: Inhibition, Activation Assay