Journal: Molecular & Cellular Proteomics : MCP

Article Title: Large-Scale Plasma Proteome Epitome Profiling is an Efficient Tool for the Discovery of Cancer Biomarkers

doi: 10.1016/j.mcpro.2023.100580

Figure Lengend Snippet: Mimotope heterogeneity and epitopes of human complement system components. SPR experiment ( A ) with BSI0442 mAb (factor H) and one of it’s cognate, biotinylated mimotope peptide (#5, immobilized). Binding inhibition curves obtained by competition with pooled human plasma ( left ) or natural CFH purified from plasma ( right ). Domain structure ( B ) of the CFH, CFHL1 and CFHR proteins, the N-terminal domains are shown in yellow . (C) The CFH concentration (µg/ml) of 37 samples was determined using selected BSI mAbs and a commercial CFH kit (Hycult) in a sandwich ELISA setup. Data for three pairwise comparisons in which seven individual samples were randomly chosen and colored (three red , four green ). Binding test summary ( D ) of QP mAbs that recognize CFH. mAb binding was tested on complete (FH), N-terminal (FH1-4), and C-terminal (FH15–20) fragments of factor H protein and on factor H related proteins (CFHR-1, CFHR-4B, and CFHR-5).

Article Snippet: The CFH concentration of selected samples was measured using a commercially available complement factor H, human, ELISA kit (Hycult Biotech), following the manufacturer’s protocol.

Techniques: Binding Assay, Inhibition, Purification, Concentration Assay, Sandwich ELISA