elisa kits  (Abcam)

 
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    Name:
    Mouse TNF alpha ELISA Kit
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    Catalog Number:
    ab100747
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    Abcam elisa kits
    Autophagy induces transforming growth factor ( <t>TGF</t> )‐β1 expression via cAMP / PKA /cAMP response element binding protein (CREB) signalling in hepatocarcinoma cells. HepG2 and BEL 7402 cells transfected with si RNA ‐control or si RNA ‐Atgs (3 and 7) were incubated in complete medium ( CM ) and HBSS for 24 h, and the cells without transfection were treated with chloroquine (5 μmol/L) or H 89 2 HC l (30 μmol/L) in complete medium and HBSS for 24 h. Expression of TGF ‐β1 in hepatocarcinoma cells was induced by autophagy induction in HBSS and was reduced by autophagy inhibition through Atg3/7 knockdown or chloroquine (5 μmol/L) treatment compared to that of cells in CM . Inhibiting PKA activation by H 89 2 HC l (30 μmol/L) significantly downregulated autophagy‐induced TGF ‐β1 expression in hepatocarcinoma cells under starvation. A, Expression of mRNA for TGF ‐β1 in HepG2 and BEL 7402 cells was determined by quantitative RT ‐ PCR . mRNA levels were normalized to that of β‐actin. B, Representative Western blots and (C) densitometric analysis for TGF ‐β1 normalized to β‐actin in HepG2 (left panel) and BEL 7402 cells (right panel). D, Representative immunofluorescence staining for TGF ‐β1 in HepG2 and BEL 7402 cells (scale bar: 10 μm, magnification ×200). E, Concentrations (pg/mL) of TGF ‐β1 in the above CM or HBSS after 24 h incubation were measured by <t>ELISA</t> . Cells cultured in CM without treatment served as the control. Data are representative images or are expressed as the mean ± SEM (n = 3) from 3 separate experiments. * P

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    1) Product Images from "Autophagy induces transforming growth factor‐β‐dependent epithelial‐mesenchymal transition in hepatocarcinoma cells through cAMP response element binding signalling. Autophagy induces transforming growth factor‐β‐dependent epithelial‐mesenchymal transition in hepatocarcinoma cells through cAMP response element binding signalling"

    Article Title: Autophagy induces transforming growth factor‐β‐dependent epithelial‐mesenchymal transition in hepatocarcinoma cells through cAMP response element binding signalling. Autophagy induces transforming growth factor‐β‐dependent epithelial‐mesenchymal transition in hepatocarcinoma cells through cAMP response element binding signalling

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.13825

    Autophagy induces transforming growth factor ( TGF )‐β1 expression via cAMP / PKA /cAMP response element binding protein (CREB) signalling in hepatocarcinoma cells. HepG2 and BEL 7402 cells transfected with si RNA ‐control or si RNA ‐Atgs (3 and 7) were incubated in complete medium ( CM ) and HBSS for 24 h, and the cells without transfection were treated with chloroquine (5 μmol/L) or H 89 2 HC l (30 μmol/L) in complete medium and HBSS for 24 h. Expression of TGF ‐β1 in hepatocarcinoma cells was induced by autophagy induction in HBSS and was reduced by autophagy inhibition through Atg3/7 knockdown or chloroquine (5 μmol/L) treatment compared to that of cells in CM . Inhibiting PKA activation by H 89 2 HC l (30 μmol/L) significantly downregulated autophagy‐induced TGF ‐β1 expression in hepatocarcinoma cells under starvation. A, Expression of mRNA for TGF ‐β1 in HepG2 and BEL 7402 cells was determined by quantitative RT ‐ PCR . mRNA levels were normalized to that of β‐actin. B, Representative Western blots and (C) densitometric analysis for TGF ‐β1 normalized to β‐actin in HepG2 (left panel) and BEL 7402 cells (right panel). D, Representative immunofluorescence staining for TGF ‐β1 in HepG2 and BEL 7402 cells (scale bar: 10 μm, magnification ×200). E, Concentrations (pg/mL) of TGF ‐β1 in the above CM or HBSS after 24 h incubation were measured by ELISA . Cells cultured in CM without treatment served as the control. Data are representative images or are expressed as the mean ± SEM (n = 3) from 3 separate experiments. * P
    Figure Legend Snippet: Autophagy induces transforming growth factor ( TGF )‐β1 expression via cAMP / PKA /cAMP response element binding protein (CREB) signalling in hepatocarcinoma cells. HepG2 and BEL 7402 cells transfected with si RNA ‐control or si RNA ‐Atgs (3 and 7) were incubated in complete medium ( CM ) and HBSS for 24 h, and the cells without transfection were treated with chloroquine (5 μmol/L) or H 89 2 HC l (30 μmol/L) in complete medium and HBSS for 24 h. Expression of TGF ‐β1 in hepatocarcinoma cells was induced by autophagy induction in HBSS and was reduced by autophagy inhibition through Atg3/7 knockdown or chloroquine (5 μmol/L) treatment compared to that of cells in CM . Inhibiting PKA activation by H 89 2 HC l (30 μmol/L) significantly downregulated autophagy‐induced TGF ‐β1 expression in hepatocarcinoma cells under starvation. A, Expression of mRNA for TGF ‐β1 in HepG2 and BEL 7402 cells was determined by quantitative RT ‐ PCR . mRNA levels were normalized to that of β‐actin. B, Representative Western blots and (C) densitometric analysis for TGF ‐β1 normalized to β‐actin in HepG2 (left panel) and BEL 7402 cells (right panel). D, Representative immunofluorescence staining for TGF ‐β1 in HepG2 and BEL 7402 cells (scale bar: 10 μm, magnification ×200). E, Concentrations (pg/mL) of TGF ‐β1 in the above CM or HBSS after 24 h incubation were measured by ELISA . Cells cultured in CM without treatment served as the control. Data are representative images or are expressed as the mean ± SEM (n = 3) from 3 separate experiments. * P

    Techniques Used: Expressing, Binding Assay, Transfection, Incubation, Inhibition, Activation Assay, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Cell Culture

    2) Product Images from "Increased receptor activator of nuclear factor κβ ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritis in vitro"

    Article Title: Increased receptor activator of nuclear factor κβ ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritis in vitro

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2016.3638

    (A) MMP-13 protein expression in cultured supernatant by enzyme-linked immunosorbent assay. *P
    Figure Legend Snippet: (A) MMP-13 protein expression in cultured supernatant by enzyme-linked immunosorbent assay. *P

    Techniques Used: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

    3) Product Images from "Allogeneic human umbilical cord-derived mesenchymal stem cells reduce lipopolysaccharide-induced inflammation and acute lung injury"

    Article Title: Allogeneic human umbilical cord-derived mesenchymal stem cells reduce lipopolysaccharide-induced inflammation and acute lung injury

    Journal: American Journal of Translational Research

    doi:

    Change of inflammatory cytokines in the BAL fluids of mice after LPS injection. The BAL fluids were collected 24 hours after delivery of vehicle (sham) or LPS (5 and 10 mg/kg) via intraperitoneal or intratracheal injection. The concentrations of the pro-inflammatory cytokines, IL-6, IL-1β and TNF-α in the BAL fluids collected from mice were detected by ELISA. Data are presented as the mean ± SD. Wilcoxon two-sample rank sum test, * P
    Figure Legend Snippet: Change of inflammatory cytokines in the BAL fluids of mice after LPS injection. The BAL fluids were collected 24 hours after delivery of vehicle (sham) or LPS (5 and 10 mg/kg) via intraperitoneal or intratracheal injection. The concentrations of the pro-inflammatory cytokines, IL-6, IL-1β and TNF-α in the BAL fluids collected from mice were detected by ELISA. Data are presented as the mean ± SD. Wilcoxon two-sample rank sum test, * P

    Techniques Used: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay

    4) Product Images from "Intravenous C16 and angiopoietin-1 improve the efficacy of placenta-derived mesenchymal stem cell therapy for EAE"

    Article Title: Intravenous C16 and angiopoietin-1 improve the efficacy of placenta-derived mesenchymal stem cell therapy for EAE

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-22867-9

    Intravenous C16 and Ang-1 enhanced the anti-inflammatory effects of PMSCs in rats with EAE as indicated in serum levels of TNF-α ( A ), IL-17 ( B ), IFN-γ ( C ) and TGF-β ( D ) at 3 and 8 weeks pi by ELISA. n = 5, # P
    Figure Legend Snippet: Intravenous C16 and Ang-1 enhanced the anti-inflammatory effects of PMSCs in rats with EAE as indicated in serum levels of TNF-α ( A ), IL-17 ( B ), IFN-γ ( C ) and TGF-β ( D ) at 3 and 8 weeks pi by ELISA. n = 5, # P

    Techniques Used: Enzyme-linked Immunosorbent Assay

    5) Product Images from "Salidroside regulates inflammatory pathway of alveolar macrophages by influencing the secretion of miRNA-146a exosomes by lung epithelial cells"

    Article Title: Salidroside regulates inflammatory pathway of alveolar macrophages by influencing the secretion of miRNA-146a exosomes by lung epithelial cells

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-77448-6

    Effects of SAL on the expression of miR-146a and TLR4 related inflammatory factors in lung tissue of rats. ( A ) Expression of miR-146a-TLR4 pathway was detected by RT-PCR. ( B ) Detection of target gene and signal pathway related gene expression by WB. ( C ) Detection of TNF-α, IL-6, IL-8 and IL-1 β inflammatory factors by ELISA.
    Figure Legend Snippet: Effects of SAL on the expression of miR-146a and TLR4 related inflammatory factors in lung tissue of rats. ( A ) Expression of miR-146a-TLR4 pathway was detected by RT-PCR. ( B ) Detection of target gene and signal pathway related gene expression by WB. ( C ) Detection of TNF-α, IL-6, IL-8 and IL-1 β inflammatory factors by ELISA.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay

    Effects of SAL on the expression of miR-146a and TLR4 related inflammatory factors in NR8383 cells. ( A ) Expression of miR-146a-TLR4 pathway was detected by RT-PCR. ( B ) Detection of target gene and signal pathway related gene expression by WB. ( C ) Detection of TNF-α, IL-6, IL-8 and IL-1 β inflammatory factors by ELISA.
    Figure Legend Snippet: Effects of SAL on the expression of miR-146a and TLR4 related inflammatory factors in NR8383 cells. ( A ) Expression of miR-146a-TLR4 pathway was detected by RT-PCR. ( B ) Detection of target gene and signal pathway related gene expression by WB. ( C ) Detection of TNF-α, IL-6, IL-8 and IL-1 β inflammatory factors by ELISA.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay

    6) Product Images from "Berberine ameliorates diabetic nephropathy by inhibiting TLR4/NF-κB pathway"

    Article Title: Berberine ameliorates diabetic nephropathy by inhibiting TLR4/NF-κB pathway

    Journal: Biological Research

    doi: 10.1186/s40659-018-0157-8

    BBR ameliorated the inflammatory response in STZ-induced DN rats. The concentrations of inflammatory factors IL-1β ( a , d ), IL-6 ( b , e ), and MCP-1 ( c , f ) in serum and renal cortex of NC group, DN group, DN + BBR (50 mg/kg) group, DN + BBR (100 mg/kg) group, and DN + BBR (200 mg/kg) group were measured by ELISA. * P
    Figure Legend Snippet: BBR ameliorated the inflammatory response in STZ-induced DN rats. The concentrations of inflammatory factors IL-1β ( a , d ), IL-6 ( b , e ), and MCP-1 ( c , f ) in serum and renal cortex of NC group, DN group, DN + BBR (50 mg/kg) group, DN + BBR (100 mg/kg) group, and DN + BBR (200 mg/kg) group were measured by ELISA. * P

    Techniques Used: Enzyme-linked Immunosorbent Assay

    7) Product Images from "Adiponectin reduces ER stress-induced apoptosis through PPARα transcriptional regulation of ATF2 in mouse adipose"

    Article Title: Adiponectin reduces ER stress-induced apoptosis through PPARα transcriptional regulation of ATF2 in mouse adipose

    Journal: Cell Death & Disease

    doi: 10.1038/cddis.2016.388

    Adiponectin alleviated adipocyte ER stress and reduced cytosolic Ca 2+ . Adipocytes were first incubated with TM; recombinant murine adiponectin was then added to the medium at 10 μ g/ml. n =3 for each treatment. ( a ) Relative AdipoR1 level in adipocytes. ( b ) Protein levels of ER stress and apoptosis. ( c ) Cytosolic Ca 2+ in adipocytes. ( d ) Flow cytometry (FCM) analysis of Cytosolic Ca 2+ in Figure 4c. ( e ) FFA level in adipocytes. ( f ) Protein levels of FATP1, CPT-1, PGC-1- α , Bax and Bcl-2 in adipocytes measured by using ELISA method. Values are means±S.D. * P
    Figure Legend Snippet: Adiponectin alleviated adipocyte ER stress and reduced cytosolic Ca 2+ . Adipocytes were first incubated with TM; recombinant murine adiponectin was then added to the medium at 10 μ g/ml. n =3 for each treatment. ( a ) Relative AdipoR1 level in adipocytes. ( b ) Protein levels of ER stress and apoptosis. ( c ) Cytosolic Ca 2+ in adipocytes. ( d ) Flow cytometry (FCM) analysis of Cytosolic Ca 2+ in Figure 4c. ( e ) FFA level in adipocytes. ( f ) Protein levels of FATP1, CPT-1, PGC-1- α , Bax and Bcl-2 in adipocytes measured by using ELISA method. Values are means±S.D. * P

    Techniques Used: Incubation, Recombinant, Flow Cytometry, Cytometry, Cycling Probe Technology, Pyrolysis Gas Chromatography, Enzyme-linked Immunosorbent Assay

    8) Product Images from "Teasaponin Ameliorates Murine Colitis by Regulating Gut Microbiota and Suppressing the Immune System Response"

    Article Title: Teasaponin Ameliorates Murine Colitis by Regulating Gut Microbiota and Suppressing the Immune System Response

    Journal: Frontiers in Medicine

    doi: 10.3389/fmed.2020.584369

    The association of microbiota induced by TS with gut inflammation. (A,B) the protein levels of IL-6 and TNF-α by ELISA; (C) the protein levels of MPO by ELISA. Data are expressed as mean ± SEM ( n = 5), statistical significance was determined with one-way ANOVA followed by Tukey test, and p -values are as follows: * p
    Figure Legend Snippet: The association of microbiota induced by TS with gut inflammation. (A,B) the protein levels of IL-6 and TNF-α by ELISA; (C) the protein levels of MPO by ELISA. Data are expressed as mean ± SEM ( n = 5), statistical significance was determined with one-way ANOVA followed by Tukey test, and p -values are as follows: * p

    Techniques Used: Enzyme-linked Immunosorbent Assay

    9) Product Images from "Differentially Expressed RNA from Public Microarray Data Identifies Serum Protein Biomarkers for Cross-Organ Transplant Rejection and Other Conditions"

    Article Title: Differentially Expressed RNA from Public Microarray Data Identifies Serum Protein Biomarkers for Cross-Organ Transplant Rejection and Other Conditions

    Journal: PLoS Computational Biology

    doi: 10.1371/journal.pcbi.1000940

    Serum ELISA results of three protein biomarkers in renal transplantation. We measured the protein concentration of ten genes by ELISA in independent serum samples of 19 AR patients and 20 patients with stable (STA) graft function after renal transplant. The protein concentrations of PECAM1 (A), CXCL9 (B), and CD44 (C) were higher in the AR serum samples, as shown in the notched boxplots. When the notches about two medians do not overlap, the medians are roughly significantly different at about a 95% confidence level [54] . The circles are outliers. P-values were calculated using the Mann-Whitney U test. One of the AR samples was inadvertently lost during the PECAM1 experiment and could not be recovered. (D) Areas under the Receiver Operating Characteristic (ROC) curves used to distinguish AR from STA were 0.811, 0.864, and 0.761 for PECAM1, CXCL9, and CD44, respectively.
    Figure Legend Snippet: Serum ELISA results of three protein biomarkers in renal transplantation. We measured the protein concentration of ten genes by ELISA in independent serum samples of 19 AR patients and 20 patients with stable (STA) graft function after renal transplant. The protein concentrations of PECAM1 (A), CXCL9 (B), and CD44 (C) were higher in the AR serum samples, as shown in the notched boxplots. When the notches about two medians do not overlap, the medians are roughly significantly different at about a 95% confidence level [54] . The circles are outliers. P-values were calculated using the Mann-Whitney U test. One of the AR samples was inadvertently lost during the PECAM1 experiment and could not be recovered. (D) Areas under the Receiver Operating Characteristic (ROC) curves used to distinguish AR from STA were 0.811, 0.864, and 0.761 for PECAM1, CXCL9, and CD44, respectively.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Transplantation Assay, Protein Concentration, MANN-WHITNEY

    Plasma ELISA of three protein biomarkers in cardiac transplantation. We measured the protein concentrations of PECAM1 (A), CXCL9 (B) and CD44 (C) by ELISA in the plasma of 32 AR patients and 31 STA patients after cardiac transplantation. All three proteins have statistically significantly higher concentration in the AR serum samples, compared to STA as shown in the notched box plots. P-values were calculated using the Mann-Whitney U test. (D) In ROC curves used to distinguish AR from STA, the areas under the curves were 0.716, 0.672, and 0.711 for PECAM1, CXCL, and CD44, respectively.
    Figure Legend Snippet: Plasma ELISA of three protein biomarkers in cardiac transplantation. We measured the protein concentrations of PECAM1 (A), CXCL9 (B) and CD44 (C) by ELISA in the plasma of 32 AR patients and 31 STA patients after cardiac transplantation. All three proteins have statistically significantly higher concentration in the AR serum samples, compared to STA as shown in the notched box plots. P-values were calculated using the Mann-Whitney U test. (D) In ROC curves used to distinguish AR from STA, the areas under the curves were 0.716, 0.672, and 0.711 for PECAM1, CXCL, and CD44, respectively.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Transplantation Assay, Concentration Assay, MANN-WHITNEY

    Identification of cross-organ AR protein biomarkers through integration of gene expression data. We integrated three microarray studies examining gene expression after rejection in the biopsy samples from pediatric renal, adult renal, and adult heart transplants (the latter two were retrieved from GEO). We identified 45 genes that were upregulated in common in acute rejection compared to stable graft function. Among ten proteins we tested by ELISA, the concentrations of three were higher in serum samples from AR patients. The concentrations of the same three proteins were also higher in AR samples from cardiac transplantation. Immunohistochemistry showed that PECAM1 was increased in AR vs. stable biopsies in renal, hepatic and cardiac transplantation. All three biomarkers were from our identified AR pathway, and two of them showed detectable protein abundance in the biofluid proteome database we constructed before. CXCL9 was not listed in our biofluid proteome database, but is known to have detectable protein abundance [24] .
    Figure Legend Snippet: Identification of cross-organ AR protein biomarkers through integration of gene expression data. We integrated three microarray studies examining gene expression after rejection in the biopsy samples from pediatric renal, adult renal, and adult heart transplants (the latter two were retrieved from GEO). We identified 45 genes that were upregulated in common in acute rejection compared to stable graft function. Among ten proteins we tested by ELISA, the concentrations of three were higher in serum samples from AR patients. The concentrations of the same three proteins were also higher in AR samples from cardiac transplantation. Immunohistochemistry showed that PECAM1 was increased in AR vs. stable biopsies in renal, hepatic and cardiac transplantation. All three biomarkers were from our identified AR pathway, and two of them showed detectable protein abundance in the biofluid proteome database we constructed before. CXCL9 was not listed in our biofluid proteome database, but is known to have detectable protein abundance [24] .

    Techniques Used: Expressing, Microarray, Enzyme-linked Immunosorbent Assay, Transplantation Assay, Immunohistochemistry, Construct

    10) Product Images from "Hepatitis B virus X protein binding to hepsin promotes C3 production by inducing IL-6 secretion from hepatocytes"

    Article Title: Hepatitis B virus X protein binding to hepsin promotes C3 production by inducing IL-6 secretion from hepatocytes

    Journal: Oncotarget

    doi: 10.18632/oncotarget.6846

    HBx binding to hepsin induces C3 production in human hepatocytes A. LO2 (left panel) and mouse primary liver cells (right panel) were transfected with flag-HBx or empty vector and C3 levels in the culture medium were measured by ELISA. Data are presented as mean ±SEM of three independent experiments. * P
    Figure Legend Snippet: HBx binding to hepsin induces C3 production in human hepatocytes A. LO2 (left panel) and mouse primary liver cells (right panel) were transfected with flag-HBx or empty vector and C3 levels in the culture medium were measured by ELISA. Data are presented as mean ±SEM of three independent experiments. * P

    Techniques Used: Binding Assay, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay

    IL-6 is the major activator of C3 production in hepatocytes A. HBx and hepsin in LO2 and mouse primary liver cells synergistically increased IL-6 secretion. LO2 and mouse primary liver cells were transfected with hepsin and HBx alone or together, and IL-6 secretion was measured by ELISA. Data are presented as mean ±SEM of three independent experiments. ** P
    Figure Legend Snippet: IL-6 is the major activator of C3 production in hepatocytes A. HBx and hepsin in LO2 and mouse primary liver cells synergistically increased IL-6 secretion. LO2 and mouse primary liver cells were transfected with hepsin and HBx alone or together, and IL-6 secretion was measured by ELISA. Data are presented as mean ±SEM of three independent experiments. ** P

    Techniques Used: Transfection, Enzyme-linked Immunosorbent Assay

    11) Product Images from "Adipose Mesenchymal Stem Cell-Derived Exosomal microRNA-1236 Reduces Resistance of Breast Cancer Cells to Cisplatin by Suppressing SLC9A1 and the Wnt/β-Catenin Signaling"

    Article Title: Adipose Mesenchymal Stem Cell-Derived Exosomal microRNA-1236 Reduces Resistance of Breast Cancer Cells to Cisplatin by Suppressing SLC9A1 and the Wnt/β-Catenin Signaling

    Journal: Cancer Management and Research

    doi: 10.2147/CMAR.S270200

    SLC9A1 activates the Wnt/β-catenin axis. ( A and B ) expression of the Wnt/β-catenin-related factors in parental and-drug resistant BC cells determined by ELISA kits. Data were presented as mean ± SD. In all panels ( A and B ), data were compared by two-way ANOVA and Tukey’s multiple comparison test. ** p
    Figure Legend Snippet: SLC9A1 activates the Wnt/β-catenin axis. ( A and B ) expression of the Wnt/β-catenin-related factors in parental and-drug resistant BC cells determined by ELISA kits. Data were presented as mean ± SD. In all panels ( A and B ), data were compared by two-way ANOVA and Tukey’s multiple comparison test. ** p

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

    Related Articles

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    Article Title: Role of the Rho/ROCK signaling pathway in the protective effects of fasudil against acute lung injury in septic rats
    Article Snippet: The protein concentrations in the BALF were detected using a protein quantification kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA), according to the manufacturer's protocol. .. The levels of TNF-α (cat. no. ab208348), IL-1β (cat. no. ab100704) and IL-6 (cat. no. ab100712) in the BALF were determined using ELISA kits (Abcam, Cambridge, UK). ..

    Article Title: Papaverine inhibits lipopolysaccharide-induced microglial activation by suppressing NF-κB signaling pathway
    Article Snippet: The production of IL1β and TNFα was measured by ELISA. .. Mouse IL1β ELISA kit (ab100705) and TNFα ELISA kit (ab100747) were purchased from Abcam Inc. (Cambridge, MA, USA). .. Western blot analysis Total proteins were prepared using RIPA lysis buffer (Sigma-Aldrich) supplemented with 1 mM phenylmethanesulfonylfluoride (PMSF, Sigma-Aldrich).

    Article Title: Hederagenin Supplementation Alleviates the Pro-Inflammatory and Apoptotic Response to Alcohol in Rats
    Article Snippet: Serum triglyceride concentrations were measured enzymatically using the Free Glycerol Determination Kit according to manufacturer’s instructions (Sigma, St. Louis, MO, USA). .. Enzyme-Linked Immunosorbent Assay (ELISA) of TNFα and IL6TNF-α and IL-6 levels in the liver were determined using mouse TNF-α and IL-6 ELISA kits (Abcam, Cambridge, UK) according to the manufacturer’s protocol. .. Reverse Transcription-PCRThe liver tissue was separated from total RNA using Trizol solution (Trizol, Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using the first strand cDNA synthesis kit (18080-051, Invitrogen, Carlsbad, CA, USA).

    Article Title: Hydrostatin-SN10 Ameliorates Pancreatitis-Induced Lung Injury by Affecting IL-6-Induced JAK2/STAT3-Associated Inflammation and Oxidative Stress
    Article Snippet: .. Measurement of Inflammatory Cytokines Mouse IL-6 ELISA kit (ab100712), mouse IL-10 ELISA kit (ab108870), and mouse TNF-α ELISA kit (ab208348) were purchased from Abcam (Boston, MA, USA). ..

    Article Title: Systemic Inflammation in C57BL/6J Mice Receiving Dietary Aluminum Sulfate; Up-Regulation of the Pro-Inflammatory Cytokines IL-6 and TNFα, C-Reactive Protein (CRP) and miRNA-146a in Blood Serum
    Article Snippet: Protein concentrations were determined using a dotMETRIC microassay as previously described (sensitivity 0.3 ng protein/ml; Invitrogen, Carlsbad, CA, USA; Chemicon-Millipore, Billerica, Massachusetts, USA) [ , , ]. .. ELISA analysis for IL-6, TNFalpha (TNFα) and C-reactive protein (CRP) ELISA analysis for the pro-inflammatory cytokines and reactive factors IL-6, TNFα and CRP were performed using a sandwich ELISA system and a mouse IL-6 ELISA Kit (ab222503; Abcam, Cambridge MA, USA; sensitivity 11.3 pg/ml; range 15.6 pg/ml–1000 pg/ml), a mouse TNF alpha ELISA Kit (ab100747, ab208348; colorimetric; sensitivity 9.1 pg/ml; range 46.88–3000 pg/ml; Abcam), and a mouse CRP ELISA Kit (PTX1) (ab157712; colorimetric, sensitivity 0.159 ug/ml; range 0.78 ug/ml–100 ug/ml; Abcam) according to the manufacturer’s instructions. .. Quality control and small non-coding RNA (sncRNA) analysis RNA was isolated using TRIzol reagent (for blood serum; a monophasic solution of phenol and guanidine isothiocyanate; Cat. No. 15596026, Invitrogen, Carlsbad, CA, USA) and samples were enriched for small RNAs using spin columns, QIAzol lysis reagent, and RNase-free reagents, buffers, RNAse inhibitors and miRNeasy Mini kits (Cat. No. 217004, Qiagen, Germantown MD, USA).

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    Article Snippet: PC-12 cells were collected following the induction of LPS for 4 h by centrifugation at 1,000 × g for 10 min at 4°C. .. TNF-α (ab100747), IL-1β (ab100704) and IL-6 (ab100712) content was measured using ELISA kits (Abcam, Cambridge, MA, USA). .. Absorbance was measured at 450 nm using a microplate reader (model 550; Bio-Rad Laboratories, Inc., Hercules, CA, USA).

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    Article Snippet: 2.17 Enzyme‐linked immunosorbent assay (ELISA) Blood was collected from the ocular venous plexus of mice after deep anaesthesia and allowed to stand at 4°C for 1 hour. .. The levels of inflammatory factors IL‐6 and TNF‐α were measured by Simple Step ELISA® Mouse Kits for IL‐6 (ab100712) and TNF‐α (ab208348) form Abcam, and the OD value was read at 450 nm using an EON spectrophotometer (BioTek Instruments). .. 2.18 Statistical analysis All data were processed using SPSS 21.0 statistical software (IBM).

    Article Title: Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies
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    Sandwich ELISA:

    Article Title: Systemic Inflammation in C57BL/6J Mice Receiving Dietary Aluminum Sulfate; Up-Regulation of the Pro-Inflammatory Cytokines IL-6 and TNFα, C-Reactive Protein (CRP) and miRNA-146a in Blood Serum
    Article Snippet: Protein concentrations were determined using a dotMETRIC microassay as previously described (sensitivity 0.3 ng protein/ml; Invitrogen, Carlsbad, CA, USA; Chemicon-Millipore, Billerica, Massachusetts, USA) [ , , ]. .. ELISA analysis for IL-6, TNFalpha (TNFα) and C-reactive protein (CRP) ELISA analysis for the pro-inflammatory cytokines and reactive factors IL-6, TNFα and CRP were performed using a sandwich ELISA system and a mouse IL-6 ELISA Kit (ab222503; Abcam, Cambridge MA, USA; sensitivity 11.3 pg/ml; range 15.6 pg/ml–1000 pg/ml), a mouse TNF alpha ELISA Kit (ab100747, ab208348; colorimetric; sensitivity 9.1 pg/ml; range 46.88–3000 pg/ml; Abcam), and a mouse CRP ELISA Kit (PTX1) (ab157712; colorimetric, sensitivity 0.159 ug/ml; range 0.78 ug/ml–100 ug/ml; Abcam) according to the manufacturer’s instructions. .. Quality control and small non-coding RNA (sncRNA) analysis RNA was isolated using TRIzol reagent (for blood serum; a monophasic solution of phenol and guanidine isothiocyanate; Cat. No. 15596026, Invitrogen, Carlsbad, CA, USA) and samples were enriched for small RNAs using spin columns, QIAzol lysis reagent, and RNase-free reagents, buffers, RNAse inhibitors and miRNeasy Mini kits (Cat. No. 217004, Qiagen, Germantown MD, USA).

    Spectrophotometry:

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  • 95
    Abcam elisa kit
    Detection of secreted albumin and blood urea nitrogen by <t>ELISA.</t> The <t>ALB</t> and BUN concentration of UC-MSCs and BM-MSCs culture supernatants collected on weeks 1, 2, 3, 4 and 5 following hepatic differentiation were measured. The medium of hepatocyte was measured as a control. Each bar represents the mean ± standard deviation (n=3). *P
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    Effect of Hericium erinaceus mycelium crude extract (HE-CE) and erinacine-S (E-S) on spinal nerve ligation (SNL)-induced elevation of plasma <t>IL-6</t> levels of mice. Plasma was collected on day 13 ( n = 5–10). ∗∗∗ p
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    Human fibrotic tissue shows altered patterns of BMP4 and <t>IGFBP4.</t> Human control peritoneum or thickened peritoneum samples from peritoneal dialysis patients were immunostained for HBME1 to identify mesothelium. Control peritoneum showed prominent mesothelial IGFBP4 and BMP4 immunostaining, which was attenuated in peritoneal dialysis samples. In the peritoneal dialysis samples, scattered IGFBP4‐positive cells were noted below the peritoneal surface. Scale bar: 100 μm.
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    Abcam elisa test kits
    Influence of quercetin on <t>STAT6</t> activation in HNEpCs after IL-4 stimulation in vitro . HNEpCs at 1 x 10 5 cells/ml are cultured with 10.0 ng/ml of IL-4 for 12 hours in the presence and absence of various concentrations of quercetin. STAT6 activation is measured using <t>ELISA,</t> and the results are expressed as the mean optical density at 450 nm ± SE of the triplicate cultures. The experiments are performed twice with similar results. Med. alone: medium alone; IL-4: interleukin-4; HNEpCs: human nasal epithelial cells.
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    Image Search Results


    Detection of secreted albumin and blood urea nitrogen by ELISA. The ALB and BUN concentration of UC-MSCs and BM-MSCs culture supernatants collected on weeks 1, 2, 3, 4 and 5 following hepatic differentiation were measured. The medium of hepatocyte was measured as a control. Each bar represents the mean ± standard deviation (n=3). *P

    Journal: Molecular Medicine Reports

    Article Title: Differentiation of umbilical cord mesenchymal stem cells into hepatocytes in comparison with bone marrow mesenchymal stem cells

    doi: 10.3892/mmr.2018.9181

    Figure Lengend Snippet: Detection of secreted albumin and blood urea nitrogen by ELISA. The ALB and BUN concentration of UC-MSCs and BM-MSCs culture supernatants collected on weeks 1, 2, 3, 4 and 5 following hepatic differentiation were measured. The medium of hepatocyte was measured as a control. Each bar represents the mean ± standard deviation (n=3). *P

    Article Snippet: ALB and blood urea nitrogen (BUN) contents were measured using ELISA kit (Human Albumin ELISA kit ab108788 and Bmassay, Human Blood Ureas Nitrogen ELISA kit 27013; Abcam, Cambridge, UK) according to the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

    Effect of Hericium erinaceus mycelium crude extract (HE-CE) and erinacine-S (E-S) on spinal nerve ligation (SNL)-induced elevation of plasma IL-6 levels of mice. Plasma was collected on day 13 ( n = 5–10). ∗∗∗ p

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effects of Hericium erinaceus Mycelium Extracts on the Functional Activity of Purinoceptors and Neuropathic Pain in Mice with L5 Spinal Nerve Ligation

    doi: 10.1155/2020/2890194

    Figure Lengend Snippet: Effect of Hericium erinaceus mycelium crude extract (HE-CE) and erinacine-S (E-S) on spinal nerve ligation (SNL)-induced elevation of plasma IL-6 levels of mice. Plasma was collected on day 13 ( n = 5–10). ∗∗∗ p

    Article Snippet: Cytokine levels were determined using a mouse IL-6 ELISA Kit (Abcam, ab100712).

    Techniques: Ligation, Mouse Assay

    Human fibrotic tissue shows altered patterns of BMP4 and IGFBP4. Human control peritoneum or thickened peritoneum samples from peritoneal dialysis patients were immunostained for HBME1 to identify mesothelium. Control peritoneum showed prominent mesothelial IGFBP4 and BMP4 immunostaining, which was attenuated in peritoneal dialysis samples. In the peritoneal dialysis samples, scattered IGFBP4‐positive cells were noted below the peritoneal surface. Scale bar: 100 μm.

    Journal: The Journal of Pathology

    Article Title: Functional molecules in mesothelial‐to‐mesenchymal transition revealed by transcriptome analyses

    doi: 10.1002/path.5101

    Figure Lengend Snippet: Human fibrotic tissue shows altered patterns of BMP4 and IGFBP4. Human control peritoneum or thickened peritoneum samples from peritoneal dialysis patients were immunostained for HBME1 to identify mesothelium. Control peritoneum showed prominent mesothelial IGFBP4 and BMP4 immunostaining, which was attenuated in peritoneal dialysis samples. In the peritoneal dialysis samples, scattered IGFBP4‐positive cells were noted below the peritoneal surface. Scale bar: 100 μm.

    Article Snippet: In contrast, neither exogenous BMP4 nor IGFBP4 prevented the loss of cell–cell cingulin localization following addition of TGF‐β1 (Figure A).

    Techniques: Immunostaining

    Application of BMP4 or IGFBP4 to TGF‐β1‐exposed rat MCs. (A) Cells were maintained for 48 h in either basal medium alone (control), medium supplemented with 1 ng/ml TGF‐β1, or the latter supplemented with either 50 ng/ml BMP4 (TGF‐β1 + BMP4) or 50 ng/ml IGFBP4 (TGF‐β1 + IGFBP4). Cells were imaged by phase contrast microscopy (top row) or, as shown in subsequent rows, by immunofluorescence for ZO1, cingulin, and αSMA, with all nuclei counterstained with 4′,6‐diamidino‐2‐phenylindole (blue). Note that exposure to either BMP4 or IGFBP4 partially preserved the cobblestone pattern of the monolayer, reduced the cytoplasmic localization of ZO1, and reduced the level of αSMA. In contrast, neither factor rescued the TGF‐β1‐induced disruption of cingulin. Scale bars: 50 μm. (B) Quantification of αSMA by immunofluorescence, showing increased immunostaining in TGF‐β1‐treated versus control cells ( n = 5; mean ±SEM). There was a reduction in αSMA expression in cells treated with TGF‐β1 + BMP4 or TGF‐β1 + IGFBP4. (C) Phase contrast images showing MC migration into a wound over a period of 16 h under different conditions. Scale bars: 200 μm. (D) Quantification of MC migration under different conditions ( n = 6; mean ±SEM). Note that TGF‐β1 exposure was associated with more extensive migration than in controls ( n = 6), and that this effect was abrogated when either BMP4 or IGFBP4 was added with TGF‐β1.

    Journal: The Journal of Pathology

    Article Title: Functional molecules in mesothelial‐to‐mesenchymal transition revealed by transcriptome analyses

    doi: 10.1002/path.5101

    Figure Lengend Snippet: Application of BMP4 or IGFBP4 to TGF‐β1‐exposed rat MCs. (A) Cells were maintained for 48 h in either basal medium alone (control), medium supplemented with 1 ng/ml TGF‐β1, or the latter supplemented with either 50 ng/ml BMP4 (TGF‐β1 + BMP4) or 50 ng/ml IGFBP4 (TGF‐β1 + IGFBP4). Cells were imaged by phase contrast microscopy (top row) or, as shown in subsequent rows, by immunofluorescence for ZO1, cingulin, and αSMA, with all nuclei counterstained with 4′,6‐diamidino‐2‐phenylindole (blue). Note that exposure to either BMP4 or IGFBP4 partially preserved the cobblestone pattern of the monolayer, reduced the cytoplasmic localization of ZO1, and reduced the level of αSMA. In contrast, neither factor rescued the TGF‐β1‐induced disruption of cingulin. Scale bars: 50 μm. (B) Quantification of αSMA by immunofluorescence, showing increased immunostaining in TGF‐β1‐treated versus control cells ( n = 5; mean ±SEM). There was a reduction in αSMA expression in cells treated with TGF‐β1 + BMP4 or TGF‐β1 + IGFBP4. (C) Phase contrast images showing MC migration into a wound over a period of 16 h under different conditions. Scale bars: 200 μm. (D) Quantification of MC migration under different conditions ( n = 6; mean ±SEM). Note that TGF‐β1 exposure was associated with more extensive migration than in controls ( n = 6), and that this effect was abrogated when either BMP4 or IGFBP4 was added with TGF‐β1.

    Article Snippet: In contrast, neither exogenous BMP4 nor IGFBP4 prevented the loss of cell–cell cingulin localization following addition of TGF‐β1 (Figure A).

    Techniques: Microscopy, Immunofluorescence, Immunostaining, Expressing, Migration

    MMT is associated with dysregulation of the BMP4 and IGF pathways. (A) Volcano plot annotated with transcripts encoding molecules implicated in BMP and IGF signalling. (B) Confirmation of decreased levels of Bmp4 and Igfbp4 as assessed by qPCR ( n = 3; mean ±SEM). (C) As determined by ELISA, the levels of BMP4 and IGFBP4 were decreased in medium conditioned by cells exposed to 1 ng/ml TGF‐β1 ( n = 5; mean ±SEM).

    Journal: The Journal of Pathology

    Article Title: Functional molecules in mesothelial‐to‐mesenchymal transition revealed by transcriptome analyses

    doi: 10.1002/path.5101

    Figure Lengend Snippet: MMT is associated with dysregulation of the BMP4 and IGF pathways. (A) Volcano plot annotated with transcripts encoding molecules implicated in BMP and IGF signalling. (B) Confirmation of decreased levels of Bmp4 and Igfbp4 as assessed by qPCR ( n = 3; mean ±SEM). (C) As determined by ELISA, the levels of BMP4 and IGFBP4 were decreased in medium conditioned by cells exposed to 1 ng/ml TGF‐β1 ( n = 5; mean ±SEM).

    Article Snippet: In contrast, neither exogenous BMP4 nor IGFBP4 prevented the loss of cell–cell cingulin localization following addition of TGF‐β1 (Figure A).

    Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Influence of quercetin on STAT6 activation in HNEpCs after IL-4 stimulation in vitro . HNEpCs at 1 x 10 5 cells/ml are cultured with 10.0 ng/ml of IL-4 for 12 hours in the presence and absence of various concentrations of quercetin. STAT6 activation is measured using ELISA, and the results are expressed as the mean optical density at 450 nm ± SE of the triplicate cultures. The experiments are performed twice with similar results. Med. alone: medium alone; IL-4: interleukin-4; HNEpCs: human nasal epithelial cells.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Suppressive Effect of Quercetin on Nitric Oxide Production from Nasal Epithelial Cells In Vitro

    doi: 10.1155/2018/6097625

    Figure Lengend Snippet: Influence of quercetin on STAT6 activation in HNEpCs after IL-4 stimulation in vitro . HNEpCs at 1 x 10 5 cells/ml are cultured with 10.0 ng/ml of IL-4 for 12 hours in the presence and absence of various concentrations of quercetin. STAT6 activation is measured using ELISA, and the results are expressed as the mean optical density at 450 nm ± SE of the triplicate cultures. The experiments are performed twice with similar results. Med. alone: medium alone; IL-4: interleukin-4; HNEpCs: human nasal epithelial cells.

    Article Snippet: Assay for Transcription Factor Activation STAT6 activity in the cultured cells was examined by measuring the phosphorylated STAT6 level using commercially available ELISA test kits (Abcam plc., Cambridge, MA, USA), according to the manufacturer's recommended procedures.

    Techniques: Activation Assay, In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay