Journal: Breast Cancer Research : BCR
Article Title: Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis
Figure Lengend Snippet: S100A4 and vascular endothelial growth factor C (VEGF-C) are transcriptionally activated by nuclear factors of activated T cells 5 (NFAT5). (A) qRT-PCR assays of the indicated transcripts in breast cancer cell lines. Relative levels compared with MCF-10A cells were plotted. (B) Western blotting analysis (left) and ELISA quantification (right) of cellular S100A4 and secreted VEGF-C, respectively, in the indicated cell lines. (C) RT-PCR (left), western blotting (S100A4) and ELISA (secreted VEGF-C) assays for indicated gene expression. (D , E) RT-PCR (D) , western blotting and ELISA (E) assays for gene expression and after transfection of MDA-MB-231 cells with indicated siRNAs. (F) Chromatin immunoprecipitation (ChIP) assay using the lysates of indicated breast cancer cells. All data are representative photographs or represent the mean ± SD of three replicates. * P
Article Snippet: ELISA assay VEGF-C protein in culture medium or patient peripheral blood samples was measured using a human VEGF-C ELISA kit (Boster, Wuhan, China).
Techniques: Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Transfection, Multiple Displacement Amplification, Chromatin Immunoprecipitation