elisa kits of vegf  (Boster Bio)

Journal: Oncology Research

Article Title: ERK Signaling Pathway Is Involved in HPV-16 E6 but not E7 Oncoprotein-Induced HIF-1α Protein Accumulation in NSCLC Cells

doi: 10.3727/096504015X14496932933610

Figure Lengend Snippet: PD98059 inhibited HPV-16 E6 but not E7 oncoprotein-induced VEGF and IL-8 protein secretion. A549 cells, transfected with HPV-16 E6 or HPV-16 E7, were exposed to various concentrations of PD98059 for 24 h. (A and B) VEGF (A) and IL-8 (B) protein concentration in the conditioned media derived from A549 cells overexpressing HPV-16 E6 oncoprotein was determined by ELISA. (C and D) VEGF (C) and IL-8 (D) protein concentration in the conditioned media derived from A549 cells overexpressing HPV-16 E7 oncoprotein was determined by ELISA. * p

Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) The concentration of VEGF and IL-8 in the conditioned media derived from treated and untreated cells was respectively determined by human VEGF ELISA Development kit and human IL-8 ELISA reagent kit (Wuhan Boster Bio-Engineering Limited Company) according to the manufacturer’s instructions.

Techniques: Transfection, Protein Concentration, Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Oncology Letters

Article Title: Cyclooxygenase-2 induces angiogenesis in pancreatic cancer mediated by prostaglandin E2

doi: 10.3892/ol.2018.8786

Figure Lengend Snippet: Celebrex treatment decreases VEGF and PGE 2 concentrations in nude mouse xenografts. (A) ELISA analysis of VEGF revealed that the concentration of VEGF in the Celebrex group (0.65±0.18 ng/mg) was decreased compared with that in the control group (1.11±0.12 ng/mg) (P

Article Snippet: VEGF proteins were assayed using human VEGF ELISA kits (cat no. EK0539; Boster Biological Technology, Pleasanton, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Cell Death & Disease

Article Title: USP12 promotes breast cancer angiogenesis by maintaining midkine stability

doi: 10.1038/s41419-021-04102-y

Figure Lengend Snippet: USP12-induced angiogenesis in breast cancer. A The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells overexpressing USP12 was detected by ELISA. B The relative expression of VEGF-A and VEGF-C in the supernatant of MDA-MB-231 cells with USP12 knockdown was detected by ELISA. C , D Migration of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( C ) and USP12 knockdown ( D ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. E , F . Tube formation assay of HUVECs treated with supernatant from MDA-MB-231 cells with USP12 overexpression ( E ) and USP12 knockdown ( F ). Representative images are shown in the left panel. The quantitative results are shown in the right panel. The experiments were repeated three times. ** p

Article Snippet: Matrigel matrix (356234, Corning), the human VEGF-A ELISA kit (EKO588-96, Boster) and the human VEGF-C ELISA KIT (EKO539-96, Boster) were also used.

Techniques: Expressing, Multiple Displacement Amplification, Enzyme-linked Immunosorbent Assay, Migration, Over Expression, Tube Formation Assay

Journal: Breast Cancer Research : BCR

Article Title: Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis

doi: 10.1186/s13058-014-0454-2

Figure Lengend Snippet: Vascular endothelial growth factor C (VEGF-C) is involved in nuclear factors of activated T cells 5 (NFAT5)-driven progression of metastatic breast cancers. (A) In vitro tube formation of human umbilical vein endothelial cells (HUVECs) after co-culture for indicated time with MDA-MB-231 cells either mocked transfected or transfected with indicated siRNAs or miRNA mimic (×100). (B - D) ELISA using the serum samples for comparison of VEGF-C levels in healthy volunteers, patients with benign lesions, and patients with breast cancers (B) , in patients with breast cancers in various stages (C) , and in patients with non-metastatic and metastatic breast cancers (D) .

Article Snippet: ELISA assay VEGF-C protein in culture medium or patient peripheral blood samples was measured using a human VEGF-C ELISA kit (Boster, Wuhan, China).

Techniques: In Vitro, Co-Culture Assay, Multiple Displacement Amplification, Transfection, Enzyme-linked Immunosorbent Assay

Journal: Breast Cancer Research : BCR

Article Title: Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis

doi: 10.1186/s13058-014-0454-2

Figure Lengend Snippet: S100A4 and vascular endothelial growth factor C (VEGF-C) are transcriptionally activated by nuclear factors of activated T cells 5 (NFAT5). (A) qRT-PCR assays of the indicated transcripts in breast cancer cell lines. Relative levels compared with MCF-10A cells were plotted. (B) Western blotting analysis (left) and ELISA quantification (right) of cellular S100A4 and secreted VEGF-C, respectively, in the indicated cell lines. (C) RT-PCR (left), western blotting (S100A4) and ELISA (secreted VEGF-C) assays for indicated gene expression. (D , E) RT-PCR (D) , western blotting and ELISA (E) assays for gene expression and after transfection of MDA-MB-231 cells with indicated siRNAs. (F) Chromatin immunoprecipitation (ChIP) assay using the lysates of indicated breast cancer cells. All data are representative photographs or represent the mean ± SD of three replicates. * P

Article Snippet: ELISA assay VEGF-C protein in culture medium or patient peripheral blood samples was measured using a human VEGF-C ELISA kit (Boster, Wuhan, China).

Techniques: Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Transfection, Multiple Displacement Amplification, Chromatin Immunoprecipitation