Structured Review

USCN Life elisa kit
RF exposure differentially induced cytokine release in microglia and astrocytes. The supernatants were collected and used for an <t>ELISA</t> test after sham and RF exposure. Naive controls (no treatment) and LPS controls (1 µg/mL) were set up at each point. (A, C, E) Releases of IL-1β, <t>TNF-α</t> and IL-6 upon sham, RF and LPS treatments in microglial N9 cells. (B, D, F) Releases of IL-1β, TNF-α and IL-6 upon sham, RF and LPS treatments in astroglial C8-D1A cells. Values are mean ± SEM for three independent experiments. * p
Elisa Kit, supplied by USCN Life, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Differential Pro-Inflammatory Responses of Astrocytes and Microglia Involve STAT3 Activation in Response to 1800 MHz Radiofrequency Fields"

Article Title: Differential Pro-Inflammatory Responses of Astrocytes and Microglia Involve STAT3 Activation in Response to 1800 MHz Radiofrequency Fields

Journal: PLoS ONE

doi: 10.1371/journal.pone.0108318

RF exposure differentially induced cytokine release in microglia and astrocytes. The supernatants were collected and used for an ELISA test after sham and RF exposure. Naive controls (no treatment) and LPS controls (1 µg/mL) were set up at each point. (A, C, E) Releases of IL-1β, TNF-α and IL-6 upon sham, RF and LPS treatments in microglial N9 cells. (B, D, F) Releases of IL-1β, TNF-α and IL-6 upon sham, RF and LPS treatments in astroglial C8-D1A cells. Values are mean ± SEM for three independent experiments. * p
Figure Legend Snippet: RF exposure differentially induced cytokine release in microglia and astrocytes. The supernatants were collected and used for an ELISA test after sham and RF exposure. Naive controls (no treatment) and LPS controls (1 µg/mL) were set up at each point. (A, C, E) Releases of IL-1β, TNF-α and IL-6 upon sham, RF and LPS treatments in microglial N9 cells. (B, D, F) Releases of IL-1β, TNF-α and IL-6 upon sham, RF and LPS treatments in astroglial C8-D1A cells. Values are mean ± SEM for three independent experiments. * p

Techniques Used: Enzyme-linked Immunosorbent Assay

2) Product Images from "Mast cell hyperactivity underpins the development of oxygen-induced retinopathy"

Article Title: Mast cell hyperactivity underpins the development of oxygen-induced retinopathy

Journal: The Journal of Clinical Investigation

doi: 10.1172/JCI89893

mMCP6 triggered retinal neovascularization. ( A ) mMCP6 ELISA was performed using serum from the OIR model and age-matched naive WT pups on P17. n = 8 in each group. * P
Figure Legend Snippet: mMCP6 triggered retinal neovascularization. ( A ) mMCP6 ELISA was performed using serum from the OIR model and age-matched naive WT pups on P17. n = 8 in each group. * P

Techniques Used: Enzyme-linked Immunosorbent Assay

3) Product Images from "Dynamin1 concentration in the prefrontal cortex is associated with cognitive impairment in Lewy body dementia"

Article Title: Dynamin1 concentration in the prefrontal cortex is associated with cognitive impairment in Lewy body dementia

Journal: F1000Research

doi: 10.12688/f1000research.3786.1

Concentration of Dynamin1 in BA9 and BA24 tissues by ELISA from subjects with PDD, DLB, AD and controls. ( A ) BA9, ( B ) BA24, PDD: Parkinson’s Disease Dementia; DLB: Dementia with Lewy Body; AD: Alzheimer’s Disease. Bars represent mean and error bars SEM.
Figure Legend Snippet: Concentration of Dynamin1 in BA9 and BA24 tissues by ELISA from subjects with PDD, DLB, AD and controls. ( A ) BA9, ( B ) BA24, PDD: Parkinson’s Disease Dementia; DLB: Dementia with Lewy Body; AD: Alzheimer’s Disease. Bars represent mean and error bars SEM.

Techniques Used: Concentration Assay, Enzyme-linked Immunosorbent Assay

4) Product Images from "Stroma-Derived Connective Tissue Growth Factor Maintains Cell Cycle Progression and Repopulation Activity of Hematopoietic Stem Cells In Vitro"

Article Title: Stroma-Derived Connective Tissue Growth Factor Maintains Cell Cycle Progression and Repopulation Activity of Hematopoietic Stem Cells In Vitro

Journal: Stem Cell Reports

doi: 10.1016/j.stemcr.2015.09.018

Decreased Stromal CTGF Decreases Repopulating HSC Activity in Culture (A) CTGF mRNA and protein content in UG26-1B6 (gray bars), Ctgf knockdown (shCtgf, black bars), and control (pLKO.1, white bars) cells as measured by qPCR and ELISA. The mean and SD of three experiments (both qPCR and ELISA) is shown. (B) Experimental design: Lin − cells (CD45.1; Figure S2 ) were co-cultured with pLKO.1 or shCtgf cells for 1 week. Each culture was then harvested and transplanted into lethally irradiated (CD45.2) primary recipients (1,000 input Lin − equivalents/recipient) together with competitor BM cells. After 16 weeks, mice were sacrificed and analyzed. Two independent experiments totaling n = 7 (for pLKO.1) and n = 9 (for shCtgf) recipients were performed. In one follow-up experiment, donor LSK cells were sorted out of the BM of 1° recipients and re-transplanted in equal numbers of 1,000 LSK cells per 2° recipient mice (pLKO.1, n = 8; shCtgf, n = 4). (C) Donor engraftment of total cells, myeloid, B and T cells in PB 5, 10, and 16 weeks after transplantation, presented as percentage of total cells. (D) Representative FACS plots displaying donor engraftment in the BM of primary recipient mice receiving co-cultured cells, 16 weeks after transplantation. (E) Engraftment of donor-derived MP and LSK cells in the BM, as percentage of total (donor plus recipient) MP and LSK cells, respectively. (F) Representative FACS plots displaying donor engraftment in the PB of secondary recipients, 16 weeks after transplantation. (G) Level of engraftment of hematopoietic cells in PB, BM, and spleen, 16 weeks after transplantation of secondary mice.
Figure Legend Snippet: Decreased Stromal CTGF Decreases Repopulating HSC Activity in Culture (A) CTGF mRNA and protein content in UG26-1B6 (gray bars), Ctgf knockdown (shCtgf, black bars), and control (pLKO.1, white bars) cells as measured by qPCR and ELISA. The mean and SD of three experiments (both qPCR and ELISA) is shown. (B) Experimental design: Lin − cells (CD45.1; Figure S2 ) were co-cultured with pLKO.1 or shCtgf cells for 1 week. Each culture was then harvested and transplanted into lethally irradiated (CD45.2) primary recipients (1,000 input Lin − equivalents/recipient) together with competitor BM cells. After 16 weeks, mice were sacrificed and analyzed. Two independent experiments totaling n = 7 (for pLKO.1) and n = 9 (for shCtgf) recipients were performed. In one follow-up experiment, donor LSK cells were sorted out of the BM of 1° recipients and re-transplanted in equal numbers of 1,000 LSK cells per 2° recipient mice (pLKO.1, n = 8; shCtgf, n = 4). (C) Donor engraftment of total cells, myeloid, B and T cells in PB 5, 10, and 16 weeks after transplantation, presented as percentage of total cells. (D) Representative FACS plots displaying donor engraftment in the BM of primary recipient mice receiving co-cultured cells, 16 weeks after transplantation. (E) Engraftment of donor-derived MP and LSK cells in the BM, as percentage of total (donor plus recipient) MP and LSK cells, respectively. (F) Representative FACS plots displaying donor engraftment in the PB of secondary recipients, 16 weeks after transplantation. (G) Level of engraftment of hematopoietic cells in PB, BM, and spleen, 16 weeks after transplantation of secondary mice.

Techniques Used: Activity Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Cell Culture, Irradiation, Mouse Assay, Transplantation Assay, FACS, Derivative Assay

Gene Expression Analysis, Gene Prioritization, and Validation of CTGF Upregulation (A) Experimental design: for each microarray hybridization, 2.5 × 10 4 sorted LSK cells were co-cultured on irradiated UG26-1B2 for 1 (d1 cc), 2 (d2 cc), and 3 (d3 cc) days (stroma/LSK ratio of approximately 10:1). Co-cultured cells were separated by the expression of SSC, CD45 and Ly6A/E (SCA-1) and used for three independent experiments including all time points, providing samples for microarray analyses (d0, n = 3; d1 mc, n = 2; d1 cc, n = 3; d2 cc, n = 3; d3 cc, n = 2), qPCR, and ELISA. (B) STEM ( Ernst and Bar-Joseph, 2006 ) analysis of dynamic expression patterns identifies 12 significant patterns, of continuously downregulated genes (four patterns, cluster C1), upregulated genes (three patterns, cluster C2), and variable up- and/or downregulation. (C) ToppFun analysis of the Biological Process GO terms enriched in cluster C1 of downregulated genes in the comparison of d0 and d1 cc UG26-1B6 stromal cells ordered by p value. For more details, see Table S1 . (D) ToppFun analysis of the Biological Process GO terms enriched in cluster C2 of upregulated genes in the comparison of d0 and d1 cc UG26-1B6 stromal cells ordered by p value. More details can be found in Table S2 . (E) Hierarchical clustering of differential gene expression data between controls (mono-cultured UG26-1B6 [d0.1, d0.2, and d0.3] and day 1 medium change [d1 mc.1 and d1 mc.2]) and UG26-1B6 cells co-cultured with LSK cells (d1 cc.1, d1 cc.2, and d1 cc.3). A list of DEGs can be found in Table S3 . (F) CTGF protein content in sorted d0, d1 mc, and d1 cc stromal cells as measured by ELISA of culture supernatants. ∗ p
Figure Legend Snippet: Gene Expression Analysis, Gene Prioritization, and Validation of CTGF Upregulation (A) Experimental design: for each microarray hybridization, 2.5 × 10 4 sorted LSK cells were co-cultured on irradiated UG26-1B2 for 1 (d1 cc), 2 (d2 cc), and 3 (d3 cc) days (stroma/LSK ratio of approximately 10:1). Co-cultured cells were separated by the expression of SSC, CD45 and Ly6A/E (SCA-1) and used for three independent experiments including all time points, providing samples for microarray analyses (d0, n = 3; d1 mc, n = 2; d1 cc, n = 3; d2 cc, n = 3; d3 cc, n = 2), qPCR, and ELISA. (B) STEM ( Ernst and Bar-Joseph, 2006 ) analysis of dynamic expression patterns identifies 12 significant patterns, of continuously downregulated genes (four patterns, cluster C1), upregulated genes (three patterns, cluster C2), and variable up- and/or downregulation. (C) ToppFun analysis of the Biological Process GO terms enriched in cluster C1 of downregulated genes in the comparison of d0 and d1 cc UG26-1B6 stromal cells ordered by p value. For more details, see Table S1 . (D) ToppFun analysis of the Biological Process GO terms enriched in cluster C2 of upregulated genes in the comparison of d0 and d1 cc UG26-1B6 stromal cells ordered by p value. More details can be found in Table S2 . (E) Hierarchical clustering of differential gene expression data between controls (mono-cultured UG26-1B6 [d0.1, d0.2, and d0.3] and day 1 medium change [d1 mc.1 and d1 mc.2]) and UG26-1B6 cells co-cultured with LSK cells (d1 cc.1, d1 cc.2, and d1 cc.3). A list of DEGs can be found in Table S3 . (F) CTGF protein content in sorted d0, d1 mc, and d1 cc stromal cells as measured by ELISA of culture supernatants. ∗ p

Techniques Used: Expressing, Microarray, Hybridization, Cell Culture, Irradiation, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

5) Product Images from "Interleukin (IL)-1β Is a Strong Inducer of IL-36γ Expression in Human Colonic Myofibroblasts"

Article Title: Interleukin (IL)-1β Is a Strong Inducer of IL-36γ Expression in Human Colonic Myofibroblasts

Journal: PLoS ONE

doi: 10.1371/journal.pone.0138423

Effects of IL-1β on IL-36γ mRNA expression in colonic myofibroblasts. (A) Dose-dependent effects of IL-1β on IL-36γ mRNA expression. The cells were incubated for 24 h with increasing concentrations of IL-1β. IL-36γ mRNA expression was expressed relative to β-actin mRNA expression (mean ± SD from 4 different experiments). (B) Time-dependent effects of IL-1β on IL-36γ mRNA expression. The cells were stimulated with IL-1β (10 ng/ml) for the pre-determined times. IL-36γ mRNA expression was expressed relative to β-actin mRNA expression (mean ± SD from 4 different experiments). (C) Dose-dependent effects of IL-1β on IL-36γ secretion. The cells were incubated for 24 h with increasing concentrations of IL-1β. IL-36γ level in supernatant was determined by ELISA (mean ± SD from 4 different experiments). (D) Time-dependent effects of IL-1β on IL-36γ secretion. The cells were stimulated with IL-1β (10 ng/ml) for the pre-determined times. IL-36γ level was determined by ELISA (mean ± SD from 4 different experiments). *P
Figure Legend Snippet: Effects of IL-1β on IL-36γ mRNA expression in colonic myofibroblasts. (A) Dose-dependent effects of IL-1β on IL-36γ mRNA expression. The cells were incubated for 24 h with increasing concentrations of IL-1β. IL-36γ mRNA expression was expressed relative to β-actin mRNA expression (mean ± SD from 4 different experiments). (B) Time-dependent effects of IL-1β on IL-36γ mRNA expression. The cells were stimulated with IL-1β (10 ng/ml) for the pre-determined times. IL-36γ mRNA expression was expressed relative to β-actin mRNA expression (mean ± SD from 4 different experiments). (C) Dose-dependent effects of IL-1β on IL-36γ secretion. The cells were incubated for 24 h with increasing concentrations of IL-1β. IL-36γ level in supernatant was determined by ELISA (mean ± SD from 4 different experiments). (D) Time-dependent effects of IL-1β on IL-36γ secretion. The cells were stimulated with IL-1β (10 ng/ml) for the pre-determined times. IL-36γ level was determined by ELISA (mean ± SD from 4 different experiments). *P

Techniques Used: Expressing, Incubation, Enzyme-linked Immunosorbent Assay

6) Product Images from "Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis"

Article Title: Celecoxib Ameliorates Portal Hypertension of the Cirrhotic Rats through the Dual Inhibitory Effects on the Intrahepatic Fibrosis and Angiogenesis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0069309

Suppression of the integrated signal pathways with celecoxib treatment. Much more positive expressions for COX-2 and p-ERK were visualized in TAA group (Immunohistochemical stain, ×400 magnifications; inserts, ×1000 magnifications). The mRNA and protein levels of hepatic COX-2 in TAA group measured with qRT-PCR and Western blot separately were the highest among three groups. The serum levels of PGE2 were measured by ELISA. The mRNAs for HIF-1α and c-fos were determined by qRT-PCR. # p
Figure Legend Snippet: Suppression of the integrated signal pathways with celecoxib treatment. Much more positive expressions for COX-2 and p-ERK were visualized in TAA group (Immunohistochemical stain, ×400 magnifications; inserts, ×1000 magnifications). The mRNA and protein levels of hepatic COX-2 in TAA group measured with qRT-PCR and Western blot separately were the highest among three groups. The serum levels of PGE2 were measured by ELISA. The mRNAs for HIF-1α and c-fos were determined by qRT-PCR. # p

Techniques Used: Immunohistochemistry, Staining, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Ursolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1/Toll-Like Receptor 4/NFκB Pathway
Article Snippet: Photomicrographs were quantified performed by converting the images to gray scale, inverting their color, and quantifying the staining intensity in each field with ImageJ software. .. The IL-1β, IL-6, and TNF-α levels in the ischemic cortex and the HMGB1 levels in the plasma samples were determined using ELISA kits (USCN Life Science Inc., Wuhan, China) according to the manufacturer’s instructions. .. Cytosolic and nuclear proteins from the ischemic cortex were prepared with the Nuclear/Cytosol Fractionation Kit (BioVision, Mountain View, CA, USA) for western blotting analysis.

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Article Title: Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer
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Article Title: Bu-Shen-Ning-Xin decoction suppresses osteoclastogenesis by modulating RANKL/OPG imbalance in the CD4+ T lymphocytes of ovariectomized mice
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for soluble RANKL (sRANKL; MTR00) were purchased from R & D Systems, Inc. (Minneapolis, MN, USA). .. ELISA kits for OPG (E108Mu) were purchased from USCN Life Sciences, Inc. (Wuhan, China). .. Leukocyte Acid Phosphatase kits were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Article Title: Copeptin level in the early prediction of cardiorenal syndrome in rats
Article Snippet: .. ELISA kits for CPP and BNP [ELISA kit for CPP was purchased from USCN Life Science, Inc., (Wuhan, China), item no. SEA365Ra and batch no. 161101216; ELISA kit for BNP with the item no. CEA541Ra and batch no. 161101217] were used to measure serum and urine CPP together with BNP levels strictly based on the kit instruction. .. Then, conventional hematoxylin and eosin (H & E) staining was performed for cardiac and renal tissues, and the tissue slices were examined under a light microscope to determine pathological changes in cardiac and renal tissues in CRS rats.

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Concentration Assay:

Article Title: Evaluation of serum procathepsin B, cystatin B and cystatin C as possible biomarkers of ovarian cancer
Article Snippet: CA-125 (CA-125-Immynoassay-Best kit, Vector, Koltzovo, Novosibirsk Region, Russia) was measured by Bio-Rad photometer, Model 68. .. Procathepsin B concentration in serum and ascetic fluid was measured by ELISA kits (R & D) for quantitative assay of human procathepsin B; cystatin B concentration was measured using ELISA kits for quantitative assay of human cystatin B level (USCN, Life Science Inc., China); and finally, cystatin C concentration was measured using ELISA kits for quantitative assay of human cystatin C (BioVendor, Czech Republic). .. Human procathepsin B immunoassay, a solid phase of ELISA, was designed to measure the pro-form of cathepsin B in different biological fluids.

Conditioned Place Preference:

Article Title: Copeptin level in the early prediction of cardiorenal syndrome in rats
Article Snippet: .. ELISA kits for CPP and BNP [ELISA kit for CPP was purchased from USCN Life Science, Inc., (Wuhan, China), item no. SEA365Ra and batch no. 161101216; ELISA kit for BNP with the item no. CEA541Ra and batch no. 161101217] were used to measure serum and urine CPP together with BNP levels strictly based on the kit instruction. .. Then, conventional hematoxylin and eosin (H & E) staining was performed for cardiac and renal tissues, and the tissue slices were examined under a light microscope to determine pathological changes in cardiac and renal tissues in CRS rats.

Marker:

Article Title: A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype in Genetically Modified Mice, A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype in Genetically Modified Mice
Article Snippet: .. ELISA The bone resorption marker CTX‐1 level in the serum was quantified in mice using commercially available ELISA kits (Uscn Life Science Inc., Wuhan, China) according to the manufacturer's instructions and the previously published protocol. .. [ ] The determination was performed in duplicate, and the plates were analyzed using an automatic microplate reader (Thermo Scientific, USA).

Mouse Assay:

Article Title: A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype in Genetically Modified Mice, A Loop‐Based and AGO‐Incorporated Virtual Screening Model Targeting AGO‐Mediated miRNA–mRNA Interactions for Drug Discovery to Rescue Bone Phenotype in Genetically Modified Mice
Article Snippet: .. ELISA The bone resorption marker CTX‐1 level in the serum was quantified in mice using commercially available ELISA kits (Uscn Life Science Inc., Wuhan, China) according to the manufacturer's instructions and the previously published protocol. .. [ ] The determination was performed in duplicate, and the plates were analyzed using an automatic microplate reader (Thermo Scientific, USA).

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    USCN Life elisa kits
    Determination of urinary <t>PDGFRB</t> concentrations in NMIBC patients pre- and post-surgery by <t>ELISA.</t> There was no significant difference between the levels of urinary PDGFRB in NMIBC patients pre-surgery and post-surgery (n = 185) (P = 0.067).
    Elisa Kits, supplied by USCN Life, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/USCN Life
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kits - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    USCN Life il 1β elisa kit
    <t>ELISA</t> was used to detect the expression levels of <t>IL-1β</t> in MSCs, EPCs, MSCs + EPCs, EPC-CM-MSCs and MSC-CM-EPCs. IL-1β was expressed in all groups at different levels. * P
    Il 1β Elisa Kit, supplied by USCN Life, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 1β elisa kit/product/USCN Life
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 1β elisa kit - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Determination of urinary PDGFRB concentrations in NMIBC patients pre- and post-surgery by ELISA. There was no significant difference between the levels of urinary PDGFRB in NMIBC patients pre-surgery and post-surgery (n = 185) (P = 0.067).

    Journal: PLoS ONE

    Article Title: Platelet-Derived Growth Factor Receptor Beta: A Novel Urinary Biomarker for Recurrence of Non-Muscle-Invasive Bladder Cancer

    doi: 10.1371/journal.pone.0096671

    Figure Lengend Snippet: Determination of urinary PDGFRB concentrations in NMIBC patients pre- and post-surgery by ELISA. There was no significant difference between the levels of urinary PDGFRB in NMIBC patients pre-surgery and post-surgery (n = 185) (P = 0.067).

    Article Snippet: Human PDGFRB protein was quantified with ELISA kits from Uscn Life Science Inc. (Wuhan, China) according the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay

    Cytokine production by PBMCs, from healthy calves after four days stimulation with PMW, PHA and Con A mitogens, as determined by ELISA. (A) for IL-2, (B) for IL-4, (C) for IL-5, (D) for IL-6, (E) for IL-10, (F) for IFN-γ, (G) for IL-17.

    Journal: Veterinary Research Forum

    Article Title: Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells

    doi:

    Figure Lengend Snippet: Cytokine production by PBMCs, from healthy calves after four days stimulation with PMW, PHA and Con A mitogens, as determined by ELISA. (A) for IL-2, (B) for IL-4, (C) for IL-5, (D) for IL-6, (E) for IL-10, (F) for IFN-γ, (G) for IL-17.

    Article Snippet: Cell culture supernatants were harvested and analyzed for cytokines using bovine IL-2, IL-4, IL-5, IL-6, IL-10, IL-17 and IFN-γ commercially available ELISA kits (all USCN Life Science Inc., Wuhan, China) and the concentration of each cytokine was measured using the ELISA reader at a wavelength of 450 nm .The limits of detection (LOD) for the individual assays were as follow: IL-2, 12.9 pgmL-1 ; IL-4, 6.2 pgmL-1 ; IL-5, 6.2 pgmL-1 ; IL-10, 12.4 pgmL-1 ; IL-17, 14.2 pgmL-1 ; IL-6, 12.6 pgmL-1 ; and IFN-γ, 12.8 pgmL-1 .

    Techniques: Enzyme-linked Immunosorbent Assay

    Levels of serum AAT, trypsin, and leptin by ELISA and liver AAT by western blot. The serum levels of AAT ( a ), trypsin ( b ), and leptin ( c ) in rats were measured by ELISA. Liver AAT were quantified by western blot ( d ).

    Journal: Scientific Reports

    Article Title: Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis

    doi: 10.1038/srep17833

    Figure Lengend Snippet: Levels of serum AAT, trypsin, and leptin by ELISA and liver AAT by western blot. The serum levels of AAT ( a ), trypsin ( b ), and leptin ( c ) in rats were measured by ELISA. Liver AAT were quantified by western blot ( d ).

    Article Snippet: AAT in patient serum samples Serum levels of AAT were measured by ELISA kits (SEB697Ra, USCN Life Science Inc.) according to the manufacturers’ instruction.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

    Patient serum AAT levels quantified by ELISA and western blot. ( a ) Serum AAT levels were measured by ELISA in 60 patients with NHSAP, 60 healthy controls, 60 patients with FLD, and 60 patients with HSAP. *P

    Journal: Scientific Reports

    Article Title: Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis

    doi: 10.1038/srep17833

    Figure Lengend Snippet: Patient serum AAT levels quantified by ELISA and western blot. ( a ) Serum AAT levels were measured by ELISA in 60 patients with NHSAP, 60 healthy controls, 60 patients with FLD, and 60 patients with HSAP. *P

    Article Snippet: AAT in patient serum samples Serum levels of AAT were measured by ELISA kits (SEB697Ra, USCN Life Science Inc.) according to the manufacturers’ instruction.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

    ELISA was used to detect the expression levels of IL-1β in MSCs, EPCs, MSCs + EPCs, EPC-CM-MSCs and MSC-CM-EPCs. IL-1β was expressed in all groups at different levels. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Interleukin-1β induces intercellular adhesion molecule-1 expression, thus enhancing the adhesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway

    doi: 10.3892/ijmm.2018.3424

    Figure Lengend Snippet: ELISA was used to detect the expression levels of IL-1β in MSCs, EPCs, MSCs + EPCs, EPC-CM-MSCs and MSC-CM-EPCs. IL-1β was expressed in all groups at different levels. * P

    Article Snippet: Subsequently, supernatants were collected and centrifuged (4°C; 5,000 × g; 10 min) in order to measure IL-1β expression using an IL-1β ELISA kit (cat. no. SEA563Mu; Uscn Life Sciences, Inc., Wuhan, China) according to the manufacturer's protocol.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing