elisa kit  (Immundiagnostik AG)

Journal: bioRxiv

Article Title: Widely used commercial ELISA does not detect preHP-2, but recognizes properdin as a potential second member of the zonulin family

doi: 10.1101/157578

Figure Lengend Snippet: Mean zonulin ELISA values obtained by testing specific candidate target proteins and patients’ serum samples. This ELISA kit did not detect increasing concentrations (range 1-50 μg/ml) of complement, recombinant zonulin (pre-HP2), mature HP1 or mature HP2. Conversely, a strong signal was detected in sera of both celiac disease patients (CD) and healthy controls (non-CD, internal control AF with HP2-2), irrespective of their HP genotype. All samples are technical triplicates, kit controls are shown for comparison.

Article Snippet: To measure zonulin, we used the commercially available ELISA kit (Immundiagnostik, Bensheim, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

Journal: bioRxiv

Article Title: Widely used commercial ELISA does not detect preHP-2, but recognizes properdin as a potential second member of the zonulin family

doi: 10.1101/157578

Figure Lengend Snippet: A) Mean zonulin ELISA values according to glucose tolerance groups B) mean ELISA values according to BMI groups. **: p

Article Snippet: To measure zonulin, we used the commercially available ELISA kit (Immundiagnostik, Bensheim, Germany).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Widely used commercial ELISA does not detect preHP-2, but recognizes properdin as a potential second member of the zonulin family

doi: 10.1101/157578

Figure Lengend Snippet: Distribution of zonulin ELISA values according to haptoglobin genotypes. Data is presented as boxplots with Turkey-Whiskers and outliers.

Article Snippet: To measure zonulin, we used the commercially available ELISA kit (Immundiagnostik, Bensheim, Germany).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Marine Drugs

Article Title: Improvement of Psoriasis by Alteration of the Gut Environment by Oral Administration of Fucoidan from Cladosiphon Okamuranus

doi: 10.3390/md18030154

Figure Lengend Snippet: Quantification of total IgA in cecal contents of fucoidan diet group and normal diet group. Volume concentrations of ( A ) m-Traf3ip2 and ( B ) wild type mouse total IgA were quantified from 63 days and 31 days, respectively, in cecum contents by ELISA. White bar shows normal diet group and black bar shows fucoidan diet group from cecum. The absorbance of reaction products was determined at 450 nm, and the production was quantified. The calculated values for the in vitro experiments are expressed as mean ± SD ( n = 5). Asterisk (*) shows significant difference ( p

Article Snippet: Total IgA was extracted from 50 mg of cecum samples and quantified using a mouse IgA ELISA quantitation kit (Immundiagnostik, Bensheim, Germany) as specified in the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, In Vitro

Journal: Scientific Reports

Article Title: Bacterial DNAemia is associated with serum zonulin levels in older subjects

doi: 10.1038/s41598-021-90476-0

Figure Lengend Snippet: Linear regression and correlation analysis carried out between blood bacterial load and serum zonulin levels. Correlation has been assessed through Pearson’s ( r coefficient), Spearman’s (ρ), and Kendall’s (τ) analyses. Analyses were performed on the first (n = 43, panels A , B,C ) and the second (n = 42, panel D , E,F ) set of blood samples collected from older people. The second set of blood samples was collected approximately four months after the first draw from the same older volunteers. Panels A and D, tables showing correlation and regression indexes and p values. Panels B and E, linear regression plot; vertical dotted lines indicate the quartiles. Panels C and F, Tukey box plots showing levels of bacterial DNAema in blood samples grouped in quartiles based on zonulin level.

Article Snippet: Serum zonulin levels were quantified using IDK® Zonulin ELISA Kit (Immundiagnostik AG, Germany).

Techniques: