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IDEXX elisa kit
Seroconversion and percentage survival of chickens experimentally infected with infectious bronchitis virus <t>(IBV),</t> People’s Republic of China. A) Survival of chickens after inoculation with IBV YN strain. B) Detection of IBV antibodies by <t>ELISA</t> at 21 days postinoculation. Cutoff titer = 396.
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1) Product Images from "Virulent Avian Infectious Bronchitis Virus, People's Republic of China"

Article Title: Virulent Avian Infectious Bronchitis Virus, People's Republic of China

Journal: Emerging Infectious Diseases

doi: 10.3201/eid1812.120552

Seroconversion and percentage survival of chickens experimentally infected with infectious bronchitis virus (IBV), People’s Republic of China. A) Survival of chickens after inoculation with IBV YN strain. B) Detection of IBV antibodies by ELISA at 21 days postinoculation. Cutoff titer = 396.
Figure Legend Snippet: Seroconversion and percentage survival of chickens experimentally infected with infectious bronchitis virus (IBV), People’s Republic of China. A) Survival of chickens after inoculation with IBV YN strain. B) Detection of IBV antibodies by ELISA at 21 days postinoculation. Cutoff titer = 396.

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay

2) Product Images from "Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus"

Article Title: Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus

Journal: Journal of Veterinary Science

doi: 10.4142/jvs.2017.18.3.307

The specific immunogenicity of recombinant Nsp7 tested by enzyme-linked immunosorbent assay. (A) Immunoreaction of the recombinant Nsp7 purified through Ni-chelating affinity chromatogr aphy with positive sera to porcine reproductive and respiratory syndrome virus (PRRSV) strain BJ-4 and HN07-1. (B–D) Immunoreaction of larger aggregate, dimer, and monomer separated by a gel filtration chromatography with positive sera to PRRSV strain BJ-4 and HN07-1.
Figure Legend Snippet: The specific immunogenicity of recombinant Nsp7 tested by enzyme-linked immunosorbent assay. (A) Immunoreaction of the recombinant Nsp7 purified through Ni-chelating affinity chromatogr aphy with positive sera to porcine reproductive and respiratory syndrome virus (PRRSV) strain BJ-4 and HN07-1. (B–D) Immunoreaction of larger aggregate, dimer, and monomer separated by a gel filtration chromatography with positive sera to PRRSV strain BJ-4 and HN07-1.

Techniques Used: Recombinant, Enzyme-linked Immunosorbent Assay, Purification, Filtration, Chromatography

3) Product Images from "Transcriptome of Porcine PBMCs over Two Generations Reveals Key Genes and Pathways Associated with Variable Antibody Responses post PRRSV Vaccination"

Article Title: Transcriptome of Porcine PBMCs over Two Generations Reveals Key Genes and Pathways Associated with Variable Antibody Responses post PRRSV Vaccination

Journal: Scientific Reports

doi: 10.1038/s41598-018-20701-w

Experimental design. Pregnant sows were vaccinated with PRRS-MLV vaccine (day 0). At 21 and 35 days after vaccination, blood samples were collected. Antibody levels in sample serum at days 21 and 35 were determined using enzyme-linked immunosorbent assay (ELISA). PBMCs were isolated from blood samples at 35 days post-vaccination and used for DEG identification. Piglets were initially vaccinated with PRRS-MLV vaccine at 28 days old. Second vaccination of PRRS-MLV was performed at 58 days old. Heparinized blood samples of piglets were collected directly at 21 days after the second vaccination (79 days old). Antibody levels of piglets were determined using ELISA. PBMCs were isolated from blood samples and used for DEG identification. HA: High antibody level; MA: Median antibody level; LA: Low antibody level.
Figure Legend Snippet: Experimental design. Pregnant sows were vaccinated with PRRS-MLV vaccine (day 0). At 21 and 35 days after vaccination, blood samples were collected. Antibody levels in sample serum at days 21 and 35 were determined using enzyme-linked immunosorbent assay (ELISA). PBMCs were isolated from blood samples at 35 days post-vaccination and used for DEG identification. Piglets were initially vaccinated with PRRS-MLV vaccine at 28 days old. Second vaccination of PRRS-MLV was performed at 58 days old. Heparinized blood samples of piglets were collected directly at 21 days after the second vaccination (79 days old). Antibody levels of piglets were determined using ELISA. PBMCs were isolated from blood samples and used for DEG identification. HA: High antibody level; MA: Median antibody level; LA: Low antibody level.

Techniques Used: Enzyme-linked Immunosorbent Assay, Isolation

4) Product Images from "The Attenuation Phenotype of a Ribavirin-Resistant Porcine Reproductive and Respiratory Syndrome Virus Is Maintained during Sequential Passages in Pigs"

Article Title: The Attenuation Phenotype of a Ribavirin-Resistant Porcine Reproductive and Respiratory Syndrome Virus Is Maintained during Sequential Passages in Pigs

Journal: Journal of Virology

doi: 10.1128/JVI.02836-15

Kinetics of the anti-PRRSV antibody (IgG) response in serum measured by ELISA (PRRS X3 Ab test; IDEXX Laboratories) in groups challenged by each virus (RVRp13, RVRp22, and MLV). Serum samples were collected from pigs in each virus group during pig-to-pig
Figure Legend Snippet: Kinetics of the anti-PRRSV antibody (IgG) response in serum measured by ELISA (PRRS X3 Ab test; IDEXX Laboratories) in groups challenged by each virus (RVRp13, RVRp22, and MLV). Serum samples were collected from pigs in each virus group during pig-to-pig

Techniques Used: Enzyme-linked Immunosorbent Assay

5) Product Images from "Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus"

Article Title: Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S218458

Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The sdAb of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect ELISA. PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.
Figure Legend Snippet: Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The sdAb of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect ELISA. PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.

Techniques Used: Binding Assay, Indirect ELISA

Schematic representation of isolating sdAbs from an immunized camel and development of sdAb-based blocking ELISA. Abbreviation: sdAb, single-domain antibody.
Figure Legend Snippet: Schematic representation of isolating sdAbs from an immunized camel and development of sdAb-based blocking ELISA. Abbreviation: sdAb, single-domain antibody.

Techniques Used: Blocking Assay, Enzyme-linked Immunosorbent Assay

Consistency between sdAb-ELISA and commercial ELISA kit. Notes: The 120 swine serum samples were detected using (A) the commercial ELISA kit and (B) sdAb-ELISA, respectively. Abbreviation: sdAb, single-domain antibody.
Figure Legend Snippet: Consistency between sdAb-ELISA and commercial ELISA kit. Notes: The 120 swine serum samples were detected using (A) the commercial ELISA kit and (B) sdAb-ELISA, respectively. Abbreviation: sdAb, single-domain antibody.

Techniques Used: Enzyme-linked Immunosorbent Assay

Evaluation of the discrepancy of testing results between sdAb-ELISA and the commercial ELISA kit by Western blot. Notes: ( A ) Western blot was performed to detect swine serum samples with discrepancy between sdAb-ELISA and the commercial ELISA kit. ( B ) The detection results of three indicated methods. Abbreviation: sdAb, single-domain antibody.
Figure Legend Snippet: Evaluation of the discrepancy of testing results between sdAb-ELISA and the commercial ELISA kit by Western blot. Notes: ( A ) Western blot was performed to detect swine serum samples with discrepancy between sdAb-ELISA and the commercial ELISA kit. ( B ) The detection results of three indicated methods. Abbreviation: sdAb, single-domain antibody.

Techniques Used: Enzyme-linked Immunosorbent Assay, Western Blot

Determination of the optimal conditions of sdAb-ELISA. Notes: ( A ) A checkerboard titration was used to test the optimal concentration range of biotinylated sdAb5 for each dilution of SIV-NP. ( B ) After the optimal concentration of biotinylated sdAb5 for each concentration SIV-NP was determined using another checkboard titration, the optimal dilution of serum was determined using sdAb-ELISA. Abbreviations: sdAb-ELISA, single-domain antibody-based blocking ELISA; SIV, swine influenza virus; NP, nucleoprotein.
Figure Legend Snippet: Determination of the optimal conditions of sdAb-ELISA. Notes: ( A ) A checkerboard titration was used to test the optimal concentration range of biotinylated sdAb5 for each dilution of SIV-NP. ( B ) After the optimal concentration of biotinylated sdAb5 for each concentration SIV-NP was determined using another checkboard titration, the optimal dilution of serum was determined using sdAb-ELISA. Abbreviations: sdAb-ELISA, single-domain antibody-based blocking ELISA; SIV, swine influenza virus; NP, nucleoprotein.

Techniques Used: Enzyme-linked Immunosorbent Assay, Titration, Concentration Assay, Blocking Assay

Specificity and sensitivity of sdAb-ELISA. Notes: ( A ) The sera against SIV, PCV2, PEDV, PRRSV, PRV, and CSFV were used to evaluate specificity of the sdAb-ELISA. ( B ) The detection limit of the commercial ELISA kit (left) and sdAb-ELISA (right) were determined for an anti-SIV reference swine positive serum. ( C ) Serum samples were diluted 1/80 and used to evaluate and compare the sensitivity of the commercial ELISA kit (top) and sdAb-ELISA (bottom). Abbreviations: SIV, swine influenza virus; PCV2, porcine circovirus type 2; PEDV, porcine epidemic diarrhea virus; PRRSV, porcine reproductive and respiratory syndrome virus; PRV, pseudorabies virus; CSFV, classical swine fever virus.
Figure Legend Snippet: Specificity and sensitivity of sdAb-ELISA. Notes: ( A ) The sera against SIV, PCV2, PEDV, PRRSV, PRV, and CSFV were used to evaluate specificity of the sdAb-ELISA. ( B ) The detection limit of the commercial ELISA kit (left) and sdAb-ELISA (right) were determined for an anti-SIV reference swine positive serum. ( C ) Serum samples were diluted 1/80 and used to evaluate and compare the sensitivity of the commercial ELISA kit (top) and sdAb-ELISA (bottom). Abbreviations: SIV, swine influenza virus; PCV2, porcine circovirus type 2; PEDV, porcine epidemic diarrhea virus; PRRSV, porcine reproductive and respiratory syndrome virus; PRV, pseudorabies virus; CSFV, classical swine fever virus.

Techniques Used: Enzyme-linked Immunosorbent Assay

6) Product Images from "Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus"

Article Title: Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S218458

Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The sdAb of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect ELISA. PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.
Figure Legend Snippet: Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The sdAb of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect ELISA. PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.

Techniques Used: Binding Assay, Indirect ELISA

Schematic representation of isolating sdAbs from an immunized camel and development of sdAb-based blocking ELISA. Abbreviation: sdAb, single-domain antibody.
Figure Legend Snippet: Schematic representation of isolating sdAbs from an immunized camel and development of sdAb-based blocking ELISA. Abbreviation: sdAb, single-domain antibody.

Techniques Used: Blocking Assay, Enzyme-linked Immunosorbent Assay

Consistency between sdAb-ELISA and commercial ELISA kit. Notes: The 120 swine serum samples were detected using (A) the commercial ELISA kit and (B) sdAb-ELISA, respectively. Abbreviation: sdAb, single-domain antibody.
Figure Legend Snippet: Consistency between sdAb-ELISA and commercial ELISA kit. Notes: The 120 swine serum samples were detected using (A) the commercial ELISA kit and (B) sdAb-ELISA, respectively. Abbreviation: sdAb, single-domain antibody.

Techniques Used: Enzyme-linked Immunosorbent Assay

Evaluation of the discrepancy of testing results between sdAb-ELISA and the commercial ELISA kit by Western blot. Notes: ( A ) Western blot was performed to detect swine serum samples with discrepancy between sdAb-ELISA and the commercial ELISA kit. ( B ) The detection results of three indicated methods. Abbreviation: sdAb, single-domain antibody.
Figure Legend Snippet: Evaluation of the discrepancy of testing results between sdAb-ELISA and the commercial ELISA kit by Western blot. Notes: ( A ) Western blot was performed to detect swine serum samples with discrepancy between sdAb-ELISA and the commercial ELISA kit. ( B ) The detection results of three indicated methods. Abbreviation: sdAb, single-domain antibody.

Techniques Used: Enzyme-linked Immunosorbent Assay, Western Blot

Determination of the optimal conditions of sdAb-ELISA. Notes: ( A ) A checkerboard titration was used to test the optimal concentration range of biotinylated sdAb5 for each dilution of SIV-NP. ( B ) After the optimal concentration of biotinylated sdAb5 for each concentration SIV-NP was determined using another checkboard titration, the optimal dilution of serum was determined using sdAb-ELISA. Abbreviations: sdAb-ELISA, single-domain antibody-based blocking ELISA; SIV, swine influenza virus; NP, nucleoprotein.
Figure Legend Snippet: Determination of the optimal conditions of sdAb-ELISA. Notes: ( A ) A checkerboard titration was used to test the optimal concentration range of biotinylated sdAb5 for each dilution of SIV-NP. ( B ) After the optimal concentration of biotinylated sdAb5 for each concentration SIV-NP was determined using another checkboard titration, the optimal dilution of serum was determined using sdAb-ELISA. Abbreviations: sdAb-ELISA, single-domain antibody-based blocking ELISA; SIV, swine influenza virus; NP, nucleoprotein.

Techniques Used: Enzyme-linked Immunosorbent Assay, Titration, Concentration Assay, Blocking Assay

Specificity and sensitivity of sdAb-ELISA. Notes: ( A ) The sera against SIV, PCV2, PEDV, PRRSV, PRV, and CSFV were used to evaluate specificity of the sdAb-ELISA. ( B ) The detection limit of the commercial ELISA kit (left) and sdAb-ELISA (right) were determined for an anti-SIV reference swine positive serum. ( C ) Serum samples were diluted 1/80 and used to evaluate and compare the sensitivity of the commercial ELISA kit (top) and sdAb-ELISA (bottom). Abbreviations: SIV, swine influenza virus; PCV2, porcine circovirus type 2; PEDV, porcine epidemic diarrhea virus; PRRSV, porcine reproductive and respiratory syndrome virus; PRV, pseudorabies virus; CSFV, classical swine fever virus.
Figure Legend Snippet: Specificity and sensitivity of sdAb-ELISA. Notes: ( A ) The sera against SIV, PCV2, PEDV, PRRSV, PRV, and CSFV were used to evaluate specificity of the sdAb-ELISA. ( B ) The detection limit of the commercial ELISA kit (left) and sdAb-ELISA (right) were determined for an anti-SIV reference swine positive serum. ( C ) Serum samples were diluted 1/80 and used to evaluate and compare the sensitivity of the commercial ELISA kit (top) and sdAb-ELISA (bottom). Abbreviations: SIV, swine influenza virus; PCV2, porcine circovirus type 2; PEDV, porcine epidemic diarrhea virus; PRRSV, porcine reproductive and respiratory syndrome virus; PRV, pseudorabies virus; CSFV, classical swine fever virus.

Techniques Used: Enzyme-linked Immunosorbent Assay

7) Product Images from "Development and application of nsp5-ELISA for the detection of antibody to infectious bronchitis virus"

Article Title: Development and application of nsp5-ELISA for the detection of antibody to infectious bronchitis virus

Journal: Journal of Virological Methods

doi: 10.1016/j.jviromet.2017.01.026

Frequency plots of the number of IFA positive (n = 595) and negative (n = 65) samples of field sera and the natural log of S/P ratios obtained from the nsp5 ELISA demonstrating two overlapping populations. The number in parentheses is the S/P ratio. The negative–positive cut-off was defined as 0.12 with an OD 450 value of a positive serum control close to 1.5 for the nsp5 ELISA, at which the DSN and DSP of the assay were greater than 90%.
Figure Legend Snippet: Frequency plots of the number of IFA positive (n = 595) and negative (n = 65) samples of field sera and the natural log of S/P ratios obtained from the nsp5 ELISA demonstrating two overlapping populations. The number in parentheses is the S/P ratio. The negative–positive cut-off was defined as 0.12 with an OD 450 value of a positive serum control close to 1.5 for the nsp5 ELISA, at which the DSN and DSP of the assay were greater than 90%.

Techniques Used: Immunofluorescence, Enzyme-linked Immunosorbent Assay

Comparative detection of antibodies to IBV in SC021202 infected (A), M41 inactivated vaccine (B) and H52 attenuated vaccine (C) immunized chickens by nsp5 ELISA and IDEXX IBV ELISA. The blue dotted lines indicate the cut-off of nsp5 ELISA; the black dotted lines indicate the cut-off of IDEXX IBV ELISA. In the IBV SC021202 infected group, 21 days post infection, only one chicken survived; therefore, there is no standard deviation indicated after 21 d.p.i. in A. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Figure Legend Snippet: Comparative detection of antibodies to IBV in SC021202 infected (A), M41 inactivated vaccine (B) and H52 attenuated vaccine (C) immunized chickens by nsp5 ELISA and IDEXX IBV ELISA. The blue dotted lines indicate the cut-off of nsp5 ELISA; the black dotted lines indicate the cut-off of IDEXX IBV ELISA. In the IBV SC021202 infected group, 21 days post infection, only one chicken survived; therefore, there is no standard deviation indicated after 21 d.p.i. in A. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Standard Deviation

8) Product Images from "Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay"

Article Title: Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Journal: Journal of Nanobiotechnology

doi: 10.1186/s12951-019-0468-0

Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)
Figure Legend Snippet: Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)

Techniques Used: Enzyme-linked Immunosorbent Assay

9) Product Images from "Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay"

Article Title: Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Journal: Journal of Nanobiotechnology

doi: 10.1186/s12951-019-0468-0

Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)
Figure Legend Snippet: Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)

Techniques Used: Enzyme-linked Immunosorbent Assay

10) Product Images from "A Peptide-Based Enzyme-Linked Immunosorbent Assay for Detecting Antibodies Against Avian Infectious Bronchitis Virus"

Article Title: A Peptide-Based Enzyme-Linked Immunosorbent Assay for Detecting Antibodies Against Avian Infectious Bronchitis Virus

Journal: Frontiers in Veterinary Science

doi: 10.3389/fvets.2020.619601

Reactivity of pELISA and positive sera against different genotype IBVs. Two colors showed the results of pELISA and commercial ELISA kits. The blue horizontal dotted line indicates the cutoff of pELISA, and the red horizontal dotted line indicates the S/ P -value of the commercial ELISA kit. pELISA had very good reaction with sera to different genotype IBV strains, but the commercial ELISA kit gave a strong reaction with only mass-type positive sera. H52 and H120 vaccine strains belong to mass type; 4/91 vaccine strain belongs to 4/91 type; QX87 vaccine strain belongs to QX-type; CK/CH/2010/JT1 virulent strain belongs to new cluster type.
Figure Legend Snippet: Reactivity of pELISA and positive sera against different genotype IBVs. Two colors showed the results of pELISA and commercial ELISA kits. The blue horizontal dotted line indicates the cutoff of pELISA, and the red horizontal dotted line indicates the S/ P -value of the commercial ELISA kit. pELISA had very good reaction with sera to different genotype IBV strains, but the commercial ELISA kit gave a strong reaction with only mass-type positive sera. H52 and H120 vaccine strains belong to mass type; 4/91 vaccine strain belongs to 4/91 type; QX87 vaccine strain belongs to QX-type; CK/CH/2010/JT1 virulent strain belongs to new cluster type.

Techniques Used: Enzyme-linked Immunosorbent Assay

11) Product Images from "A Peptide-Based Enzyme-Linked Immunosorbent Assay for Detecting Antibodies Against Avian Infectious Bronchitis Virus"

Article Title: A Peptide-Based Enzyme-Linked Immunosorbent Assay for Detecting Antibodies Against Avian Infectious Bronchitis Virus

Journal: Frontiers in Veterinary Science

doi: 10.3389/fvets.2020.619601

Reactivity of pELISA and positive sera against different genotype IBVs. Two colors showed the results of pELISA and commercial ELISA kits. The blue horizontal dotted line indicates the cutoff of pELISA, and the red horizontal dotted line indicates the S/ P -value of the commercial ELISA kit. pELISA had very good reaction with sera to different genotype IBV strains, but the commercial ELISA kit gave a strong reaction with only mass-type positive sera. H52 and H120 vaccine strains belong to mass type; 4/91 vaccine strain belongs to 4/91 type; QX87 vaccine strain belongs to QX-type; CK/CH/2010/JT1 virulent strain belongs to new cluster type.
Figure Legend Snippet: Reactivity of pELISA and positive sera against different genotype IBVs. Two colors showed the results of pELISA and commercial ELISA kits. The blue horizontal dotted line indicates the cutoff of pELISA, and the red horizontal dotted line indicates the S/ P -value of the commercial ELISA kit. pELISA had very good reaction with sera to different genotype IBV strains, but the commercial ELISA kit gave a strong reaction with only mass-type positive sera. H52 and H120 vaccine strains belong to mass type; 4/91 vaccine strain belongs to 4/91 type; QX87 vaccine strain belongs to QX-type; CK/CH/2010/JT1 virulent strain belongs to new cluster type.

Techniques Used: Enzyme-linked Immunosorbent Assay

12) Product Images from "Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay"

Article Title: Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Journal: Journal of Nanobiotechnology

doi: 10.1186/s12951-019-0468-0

Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)
Figure Legend Snippet: Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)

Techniques Used: Enzyme-linked Immunosorbent Assay

13) Product Images from "Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay"

Article Title: Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Journal: Journal of Nanobiotechnology

doi: 10.1186/s12951-019-0468-0

Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)
Figure Legend Snippet: Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)

Techniques Used: Enzyme-linked Immunosorbent Assay

Screening the nanobodies against the NDV-NP protein. a Titres of antibodies against NDV-NP protein in the sera from the camel after the fifth immunisation. b Alignment of amino acid sequence of 9 screened nanobodies. Numbering and CDRs are according to the previous methods [ 4 ]. The residues at positions 37, 44, 45, and 47 are indicated by red arrows. c Specific reactions between the 9 screened nanobodies and NDV-NP protein. d Titration of the 9 screened nanobodies binding with the NDV-NP protein. e Analysis of the 9 screened nanobodies blocking the binding between the chicken sera and NDV-NP protein by blocking ELISA
Figure Legend Snippet: Screening the nanobodies against the NDV-NP protein. a Titres of antibodies against NDV-NP protein in the sera from the camel after the fifth immunisation. b Alignment of amino acid sequence of 9 screened nanobodies. Numbering and CDRs are according to the previous methods [ 4 ]. The residues at positions 37, 44, 45, and 47 are indicated by red arrows. c Specific reactions between the 9 screened nanobodies and NDV-NP protein. d Titration of the 9 screened nanobodies binding with the NDV-NP protein. e Analysis of the 9 screened nanobodies blocking the binding between the chicken sera and NDV-NP protein by blocking ELISA

Techniques Used: Sequencing, Titration, Binding Assay, Blocking Assay, Enzyme-linked Immunosorbent Assay

Identification of NDV-Nb5-HRP fusion protein expression and secretion in the HEK293T cells. a Detection of NDV-Nb5-HRP expressed in the HEK293T cells with the anti-HA mAb as the first antibody by IFA. b Detection of the HRP activity in the NDV-Nb5-HRP fusions secreted into the culture medium of HEK293T cells. c Detection of the NDV-Nb5-HRP reaction with the NDV-NP protein using indirect ELISA
Figure Legend Snippet: Identification of NDV-Nb5-HRP fusion protein expression and secretion in the HEK293T cells. a Detection of NDV-Nb5-HRP expressed in the HEK293T cells with the anti-HA mAb as the first antibody by IFA. b Detection of the HRP activity in the NDV-Nb5-HRP fusions secreted into the culture medium of HEK293T cells. c Detection of the NDV-Nb5-HRP reaction with the NDV-NP protein using indirect ELISA

Techniques Used: Expressing, Immunofluorescence, Activity Assay, Indirect ELISA

14) Product Images from "Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay"

Article Title: Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

Journal: Journal of Nanobiotechnology

doi: 10.1186/s12951-019-0468-0

Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)
Figure Legend Snippet: Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)

Techniques Used: Enzyme-linked Immunosorbent Assay

15) Product Images from "Postnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implications"

Article Title: Postnatal Persistent Infection with Classical Swine Fever Virus and Its Immunological Implications

Journal: PLoS ONE

doi: 10.1371/journal.pone.0125692

Humoral and cellular immune response against CSFV infection. (a) Antibody response to E2 glycoprotein detected by ELISA (IDEXX) after infection (in blocking %). Values greater than 40% blocking were considered positive. (b) Neutralising antibody titres against PR (pigs 1 to 14 and sow PR) and Cat01 (pigs 16 to 28 and sow Cat) CSFV strains at 3 and 4 weeks p.i. (c) Lack of IFN- γ response by ELISPOT assay in CSFV postnatally persistently infected piglets and detection of effective response in immunocompetent pigs from the PR group at 4 weeks p.i. A total of 5x10 5 PBMCs/well were plated in triplicates at 0.1 multiplicity of infection (MOI) of CSFV homologous strains: PR strain (samples from pigs 1 to 14 and sow PR) and Cat01 strain (samples from pigs 17 to 27 and sow Cat). Moreover, the samples were incubated in the presence of Thiverval strain at 0.01 MOI and phytohaemagglutinin (PHA) (10 μg/ml). Asterisk symbol indicates the sample from pig 28 analysed at 19 dpi.
Figure Legend Snippet: Humoral and cellular immune response against CSFV infection. (a) Antibody response to E2 glycoprotein detected by ELISA (IDEXX) after infection (in blocking %). Values greater than 40% blocking were considered positive. (b) Neutralising antibody titres against PR (pigs 1 to 14 and sow PR) and Cat01 (pigs 16 to 28 and sow Cat) CSFV strains at 3 and 4 weeks p.i. (c) Lack of IFN- γ response by ELISPOT assay in CSFV postnatally persistently infected piglets and detection of effective response in immunocompetent pigs from the PR group at 4 weeks p.i. A total of 5x10 5 PBMCs/well were plated in triplicates at 0.1 multiplicity of infection (MOI) of CSFV homologous strains: PR strain (samples from pigs 1 to 14 and sow PR) and Cat01 strain (samples from pigs 17 to 27 and sow Cat). Moreover, the samples were incubated in the presence of Thiverval strain at 0.01 MOI and phytohaemagglutinin (PHA) (10 μg/ml). Asterisk symbol indicates the sample from pig 28 analysed at 19 dpi.

Techniques Used: Infection, Enzyme-linked Immunosorbent Assay, Blocking Assay, Enzyme-linked Immunospot, Incubation

16) Product Images from "Simple Indirect Enzyme-Linked Immunosorbent Assay to Detect Antibodies Against Bovine Viral Diarrhea Virus, Based on Prokaryotically Expressed Recombinant MBP-NS3 Protein"

Article Title: Simple Indirect Enzyme-Linked Immunosorbent Assay to Detect Antibodies Against Bovine Viral Diarrhea Virus, Based on Prokaryotically Expressed Recombinant MBP-NS3 Protein

Journal: Jundishapur Journal of Microbiology

doi: 10.5812/jjm.14311

The Comparison of MBP-NS3-ELISA and Virus Neutralization Test Results The horizontal dashed line represents the cut-off value (0.303) and divides the tested samples into two groups based on the OD values of MBP-NS3-ELISA. The vertical dashed line divides the tested samples into two groups based on the results of VNT.
Figure Legend Snippet: The Comparison of MBP-NS3-ELISA and Virus Neutralization Test Results The horizontal dashed line represents the cut-off value (0.303) and divides the tested samples into two groups based on the OD values of MBP-NS3-ELISA. The vertical dashed line divides the tested samples into two groups based on the results of VNT.

Techniques Used: Enzyme-linked Immunosorbent Assay, Neutralization

The Comparison of MBP-NS3-ELISA and Commercial ELISA Kit Results The horizontal dashed line represents the cut-off value (0.303) and divides the tested samples into two groups based on the OD values of MBP-NS3-ELISA. The vertical dashed line divides the tested samples into two groups based on the results of the commercial ELISA kit.
Figure Legend Snippet: The Comparison of MBP-NS3-ELISA and Commercial ELISA Kit Results The horizontal dashed line represents the cut-off value (0.303) and divides the tested samples into two groups based on the OD values of MBP-NS3-ELISA. The vertical dashed line divides the tested samples into two groups based on the results of the commercial ELISA kit.

Techniques Used: Enzyme-linked Immunosorbent Assay

17) Product Images from "Comparative Genomics of Korean Infectious Bronchitis Viruses (IBVs) and an Animal Model to Evaluate Pathogenicity of IBVs to the Reproductive Organs "

Article Title: Comparative Genomics of Korean Infectious Bronchitis Viruses (IBVs) and an Animal Model to Evaluate Pathogenicity of IBVs to the Reproductive Organs

Journal: Viruses

doi: 10.3390/v4112670

Mean enzyme-linked immunosorbent assay (ELISA) antibody titers before and after challenge in experimental chickens vaccinated with an inactivated IB oil vaccine at 13-week-old and challenged with IBV SNU8067 3 weeks later. The sera were collected at 5 weeks after challenge. The levels of antibody titers between the control and vaccinated groups after vaccination and challenge were significantly different ( P
Figure Legend Snippet: Mean enzyme-linked immunosorbent assay (ELISA) antibody titers before and after challenge in experimental chickens vaccinated with an inactivated IB oil vaccine at 13-week-old and challenged with IBV SNU8067 3 weeks later. The sera were collected at 5 weeks after challenge. The levels of antibody titers between the control and vaccinated groups after vaccination and challenge were significantly different ( P

Techniques Used: Enzyme-linked Immunosorbent Assay

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Article Snippet: The sensitivity of the sdAb-ELISA was determined using the anti-SIV positive reference serum with different dilutions and compared with a commercial ELISA kit. .. The limit of detection of sdAb-ELISA reached 1:160, which is lower than that of commercial ELISA kit (1:20) ( ). .. To further evaluate the sensitivity of the sdAb-ELISA, 78 commercial ELISA-anti-SIV positive serum samples (diluted 1:10) (data not shown) were diluted 1:80 to reduce the antibody level and tested again using the commercial ELISA kit and the sdAb-ELISA.

Article Title: The phosphorylation of the N protein could affect PRRSV virulence in vivo.
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Derivative Assay:

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Article Title: The phosphorylation of the N protein could affect PRRSV virulence in vivo.
Article Snippet: .. All piglets were confirmed to be negative for PRV, CSFV, SIV, PCV2 and PRRSV by commercial IDEXX ELISA kits and RT-PCR. ..

Article Title: The phosphorylation of the N protein could affect PRRSV virulence in vivo
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    IDEXX prrsv antibody enzyme linked immunosorbent assay elisa kit
    Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked <t>immunosorbent</t> assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p
    Prrsv Antibody Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by IDEXX, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prrsv antibody enzyme linked immunosorbent assay elisa kit/product/IDEXX
    Average 86 stars, based on 1 article reviews
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    prrsv antibody enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2021-03
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    99
    IDEXX elisa kit ibv ab test
    Interactive dot diagram based on <t>ELISA-rN</t> outcomes in relation to ELISA kit IDEXX <t>IBV</t> Ab Test. The results of the commercial test ELISA kit IDEXX IBV Ab Test (IDEXX) were compared with that of the ELISA-rN, confirming 90.16% sensitivity and 90.34% specificity
    Elisa Kit Ibv Ab Test, supplied by IDEXX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit ibv ab test/product/IDEXX
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    IDEXX elisa kit
    Correlation between <t>cELISA</t> and the HI test and the commercial <t>ELISA</t> kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)
    Elisa Kit, supplied by IDEXX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p

    Journal: Journal of Veterinary Science

    Article Title: Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages

    doi: 10.4142/jvs.2018.19.3.358

    Figure Lengend Snippet: Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p

    Article Snippet: PRRSV-specific antibodies were detected by using a commercial PRRSV antibody enzyme-linked immunosorbent assay (ELISA) kit (HerdChek PRRS X3; IDEXX Laboratories, USA), according to the manufacturer's protocols, and a serum neutralization test as described previously [ ].

    Techniques: In Vivo, Infection, Enzyme-linked Immunosorbent Assay

    Interactive dot diagram based on ELISA-rN outcomes in relation to ELISA kit IDEXX IBV Ab Test. The results of the commercial test ELISA kit IDEXX IBV Ab Test (IDEXX) were compared with that of the ELISA-rN, confirming 90.16% sensitivity and 90.34% specificity

    Journal: Virology Journal

    Article Title: Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis

    doi: 10.1186/s12985-018-1096-2

    Figure Lengend Snippet: Interactive dot diagram based on ELISA-rN outcomes in relation to ELISA kit IDEXX IBV Ab Test. The results of the commercial test ELISA kit IDEXX IBV Ab Test (IDEXX) were compared with that of the ELISA-rN, confirming 90.16% sensitivity and 90.34% specificity

    Article Snippet: The serum samples were kindly provided by MercoLab Laboratories (Garibaldi, Brazil), a laboratory accredited by the MAPA (Ministry of Agriculture, Livestock and Food Supply) that uses the commercial ELISA kit IBV Ab Test (IDEXX) for characterization.

    Techniques: Enzyme-linked Immunosorbent Assay

    Western blot analyses of the avian serum samples that scored negative for the IDEXX IBV Ab Test and positive in the ELISA-rN. Lane 1: Spectra Multicolor Broad Range Protein Ladder (ThermoFisher Scientific); Lanes 2–15: Serum samples with discrepant ELISA results

    Journal: Virology Journal

    Article Title: Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis

    doi: 10.1186/s12985-018-1096-2

    Figure Lengend Snippet: Western blot analyses of the avian serum samples that scored negative for the IDEXX IBV Ab Test and positive in the ELISA-rN. Lane 1: Spectra Multicolor Broad Range Protein Ladder (ThermoFisher Scientific); Lanes 2–15: Serum samples with discrepant ELISA results

    Article Snippet: The serum samples were kindly provided by MercoLab Laboratories (Garibaldi, Brazil), a laboratory accredited by the MAPA (Ministry of Agriculture, Livestock and Food Supply) that uses the commercial ELISA kit IBV Ab Test (IDEXX) for characterization.

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

    Evaluation of different batches of rN protein production used as antigen in the indirect ELISA development and tested with positive and negative sera for IBV. Sample 1: strongly reactive positive, samples 2–5: moderately reactive positive and samples 6–9: negative serum samples

    Journal: Virology Journal

    Article Title: Combined use of ELISA and Western blot with recombinant N protein is a powerful tool for the immunodiagnosis of avian infectious bronchitis

    doi: 10.1186/s12985-018-1096-2

    Figure Lengend Snippet: Evaluation of different batches of rN protein production used as antigen in the indirect ELISA development and tested with positive and negative sera for IBV. Sample 1: strongly reactive positive, samples 2–5: moderately reactive positive and samples 6–9: negative serum samples

    Article Snippet: The serum samples were kindly provided by MercoLab Laboratories (Garibaldi, Brazil), a laboratory accredited by the MAPA (Ministry of Agriculture, Livestock and Food Supply) that uses the commercial ELISA kit IBV Ab Test (IDEXX) for characterization.

    Techniques: Indirect ELISA

    Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)

    Journal: Journal of Nanobiotechnology

    Article Title: Nanobody-horseradish peroxidase fusion protein as an ultrasensitive probe to detect antibodies against Newcastle disease virus in the immunoassay

    doi: 10.1186/s12951-019-0468-0

    Figure Lengend Snippet: Correlation between cELISA and the HI test and the commercial ELISA kit (IDEXX). a Serum titers for antibodies against NDV detected between the cELISA (PI) and HI test (log2). b Titres of antibodies against NDV in different dilution serum samples detected between the cELISA (PI) and commercial ELISA kit test (S/P)

    Article Snippet: Compared with the HI test and a commercial ELISA kit, the developed cELISA exhibits higher sensitivity, specificity, simplicity, and rapid detection times (only approximately 1 h).

    Techniques: Enzyme-linked Immunosorbent Assay

    The Comparison of MBP-NS3-ELISA and Virus Neutralization Test Results The horizontal dashed line represents the cut-off value (0.303) and divides the tested samples into two groups based on the OD values of MBP-NS3-ELISA. The vertical dashed line divides the tested samples into two groups based on the results of VNT.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Simple Indirect Enzyme-Linked Immunosorbent Assay to Detect Antibodies Against Bovine Viral Diarrhea Virus, Based on Prokaryotically Expressed Recombinant MBP-NS3 Protein

    doi: 10.5812/jjm.14311

    Figure Lengend Snippet: The Comparison of MBP-NS3-ELISA and Virus Neutralization Test Results The horizontal dashed line represents the cut-off value (0.303) and divides the tested samples into two groups based on the OD values of MBP-NS3-ELISA. The vertical dashed line divides the tested samples into two groups based on the results of VNT.

    Article Snippet: The relative sensitivity and specificity of MBP-NS3-ELISA with respect to commercial ELISA kit were 90.72% and 91.15%, respectively; the accuracy of the developed MBP-NS3-ELISA was 91%.

    Techniques: Enzyme-linked Immunosorbent Assay, Neutralization

    The Comparison of MBP-NS3-ELISA and Commercial ELISA Kit Results The horizontal dashed line represents the cut-off value (0.303) and divides the tested samples into two groups based on the OD values of MBP-NS3-ELISA. The vertical dashed line divides the tested samples into two groups based on the results of the commercial ELISA kit.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Simple Indirect Enzyme-Linked Immunosorbent Assay to Detect Antibodies Against Bovine Viral Diarrhea Virus, Based on Prokaryotically Expressed Recombinant MBP-NS3 Protein

    doi: 10.5812/jjm.14311

    Figure Lengend Snippet: The Comparison of MBP-NS3-ELISA and Commercial ELISA Kit Results The horizontal dashed line represents the cut-off value (0.303) and divides the tested samples into two groups based on the OD values of MBP-NS3-ELISA. The vertical dashed line divides the tested samples into two groups based on the results of the commercial ELISA kit.

    Article Snippet: The relative sensitivity and specificity of MBP-NS3-ELISA with respect to commercial ELISA kit were 90.72% and 91.15%, respectively; the accuracy of the developed MBP-NS3-ELISA was 91%.

    Techniques: Enzyme-linked Immunosorbent Assay