tnf α elisa kit  (Danaher Inc)


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    Danaher Inc tnf α elisa kit
    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), <t>and</t> <t>TNF-α</t> (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Tnf α Elisa Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α elisa kit/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf α elisa kit - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance
    Figure Legend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    aspartate aminotransferase ast elisa kit  (Cusabio)


    Bioz Manufacturer Symbol Cusabio manufactures this product  
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    Cusabio aspartate aminotransferase ast elisa kit
    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and <t>AST</t> (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using <t>ELISA.</t> C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Aspartate Aminotransferase Ast Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase ast elisa kit/product/Cusabio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase ast elisa kit - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance
    Figure Legend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy


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    Jiancheng Inc elisa kits
    Elisa Kits, supplied by Jiancheng Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    aspartate aminotransferase ast elisa kit  (Cusabio)


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    Cusabio aspartate aminotransferase ast elisa kit
    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and <t>AST</t> (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using <t>ELISA.</t> C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Aspartate Aminotransferase Ast Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase ast elisa kit/product/Cusabio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase ast elisa kit - by Bioz Stars, 2024-07
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    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance
    Figure Legend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    il 6 elisa kit  (Danaher Inc)


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    Danaher Inc il 6 elisa kit
    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using <t>ELISA.</t> C The release of pro-inflammatory <t>cytokines</t> <t>IL-6</t> (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Il 6 Elisa Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 6 elisa kit/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 6 elisa kit - by Bioz Stars, 2024-07
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    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance
    Figure Legend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    ifn γ elisa kit  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
    Bioz Manufacturer Symbol Danaher Inc manufactures this product  
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    Structured Review

    Danaher Inc ifn γ elisa kit
    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), <t>IFN-γ</t> (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Ifn γ Elisa Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ifn γ elisa kit/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ifn γ elisa kit - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance
    Figure Legend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    diamine oxidase dao elisa kit  (Cusabio)


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    Cusabio diamine oxidase dao elisa kit
    The gut barrier function restored by SP is impaired by Veillonella parvula. A LPS levels (F (DFn, DFd) = 2.609 (5, 42), p = 0.0384) in mouse colon tissues were examined using <t>ELISA.</t> B <t>DAO</t> levels (F (DFn, DFd) = 1.373 (5, 42), p = 0.2537) in mouse serum were examined using ELISA. C Analysis of D-Lactate (F (DFn, DFd) = 2.825 (5, 42), p = 0.0275) in mouse serum by colorimetric method. D-F Immunofluorescence detection of Claudin-1 (F (DFn, DFd) = 1.295 (5, 42), p = 0.2843), Jam-a (F DFn, DFd) = 0.8971 (5, 42), p = 0.4919), and Occludin (F (DFn, DFd) = 0.5617 (5, 42), p = 0.7287) expression in mouse colon tissues. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (B, D, E, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (A, C), and Games-Howell's multiple comparisons test was used for post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Diamine Oxidase Dao Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diamine oxidase dao elisa kit/product/Cusabio
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    diamine oxidase dao elisa kit - by Bioz Stars, 2024-07
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    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    The gut barrier function restored by SP is impaired by Veillonella parvula. A LPS levels (F (DFn, DFd) = 2.609 (5, 42), p = 0.0384) in mouse colon tissues were examined using ELISA. B DAO levels (F (DFn, DFd) = 1.373 (5, 42), p = 0.2537) in mouse serum were examined using ELISA. C Analysis of D-Lactate (F (DFn, DFd) = 2.825 (5, 42), p = 0.0275) in mouse serum by colorimetric method. D-F Immunofluorescence detection of Claudin-1 (F (DFn, DFd) = 1.295 (5, 42), p = 0.2843), Jam-a (F DFn, DFd) = 0.8971 (5, 42), p = 0.4919), and Occludin (F (DFn, DFd) = 0.5617 (5, 42), p = 0.7287) expression in mouse colon tissues. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (B, D, E, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (A, C), and Games-Howell's multiple comparisons test was used for post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: The gut barrier function restored by SP is impaired by Veillonella parvula. A LPS levels (F (DFn, DFd) = 2.609 (5, 42), p = 0.0384) in mouse colon tissues were examined using ELISA. B DAO levels (F (DFn, DFd) = 1.373 (5, 42), p = 0.2537) in mouse serum were examined using ELISA. C Analysis of D-Lactate (F (DFn, DFd) = 2.825 (5, 42), p = 0.0275) in mouse serum by colorimetric method. D-F Immunofluorescence detection of Claudin-1 (F (DFn, DFd) = 1.295 (5, 42), p = 0.2843), Jam-a (F DFn, DFd) = 0.8971 (5, 42), p = 0.4919), and Occludin (F (DFn, DFd) = 0.5617 (5, 42), p = 0.7287) expression in mouse colon tissues. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (B, D, E, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (A, C), and Games-Howell's multiple comparisons test was used for post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunofluorescence, Expressing

    tnf α elisa kit  (Danaher Inc)


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    Danaher Inc tnf α elisa kit
    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), <t>and</t> <t>TNF-α</t> (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Tnf α Elisa Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tnf α elisa kit/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tnf α elisa kit - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance"

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    Journal: Cellular and Molecular Life Sciences: CMLS

    doi: 10.1007/s00018-023-05055-5

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Figure Legend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Techniques Used: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance
    Figure Legend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Techniques Used: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
    Figure Legend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy


    Structured Review

    MyBiosource Biotechnology immunosorbent assay elisa kit
    MiR-502-3p modulates GABRα1 levels. (A) Firefly/Renilla dual luciferase assay of hippocampal neurons (HT22) cells transfected with GABRα1 3’-UTR construct, control construct, miR-502-3p agomiRs and miR-502-3p antagomiRs. The luciferase activity was significantly reduced in the cells co-transfected with GABRα1 3’-UTR construct and miR-502-3p agomiRs relative to other groups. (B) qRT-PCR analysis of miR-502-3p in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. MiR-502-3p expression was significantly increased in the miR-502-3p agomiRs transfected cells, while it was decreased in miR-502-3p antagomiRs transfected cells compared with scramble control. (C) qRT-PCR analysis of GABRα1 mRNA in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 expression was decreased significantly in the miR-502-3p agomiRs transfected cells, while its levels were increased significantly in miR-502-3p antagomiRs transfected cells compared with scramble control. (D) Immunoblot analysis of GABRα1 protein in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 protein levels were decreased in the miR-502-3p agomiRs transfected cells. (E) Densitometry analysis of GABRα1 blot shows a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiRs show an increased level of GABRα1 protein. (F) <t>Enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> showed a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs treated cells compared with scramble control and antagomiRs treated cells. For statistical analysis, one-way analysis of variance was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. GABRα1: Gamma-aminobutyric acid type A receptor subunit alpha1; miR: microRNA; qRT-PCR: quantitative reverse transcription-polymerase chain reaction.
    Immunosorbent Assay Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons"

    Article Title: MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-23-01064

    MiR-502-3p modulates GABRα1 levels. (A) Firefly/Renilla dual luciferase assay of hippocampal neurons (HT22) cells transfected with GABRα1 3’-UTR construct, control construct, miR-502-3p agomiRs and miR-502-3p antagomiRs. The luciferase activity was significantly reduced in the cells co-transfected with GABRα1 3’-UTR construct and miR-502-3p agomiRs relative to other groups. (B) qRT-PCR analysis of miR-502-3p in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. MiR-502-3p expression was significantly increased in the miR-502-3p agomiRs transfected cells, while it was decreased in miR-502-3p antagomiRs transfected cells compared with scramble control. (C) qRT-PCR analysis of GABRα1 mRNA in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 expression was decreased significantly in the miR-502-3p agomiRs transfected cells, while its levels were increased significantly in miR-502-3p antagomiRs transfected cells compared with scramble control. (D) Immunoblot analysis of GABRα1 protein in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 protein levels were decreased in the miR-502-3p agomiRs transfected cells. (E) Densitometry analysis of GABRα1 blot shows a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiRs show an increased level of GABRα1 protein. (F) Enzyme-linked immunosorbent assay showed a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs treated cells compared with scramble control and antagomiRs treated cells. For statistical analysis, one-way analysis of variance was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. GABRα1: Gamma-aminobutyric acid type A receptor subunit alpha1; miR: microRNA; qRT-PCR: quantitative reverse transcription-polymerase chain reaction.
    Figure Legend Snippet: MiR-502-3p modulates GABRα1 levels. (A) Firefly/Renilla dual luciferase assay of hippocampal neurons (HT22) cells transfected with GABRα1 3’-UTR construct, control construct, miR-502-3p agomiRs and miR-502-3p antagomiRs. The luciferase activity was significantly reduced in the cells co-transfected with GABRα1 3’-UTR construct and miR-502-3p agomiRs relative to other groups. (B) qRT-PCR analysis of miR-502-3p in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. MiR-502-3p expression was significantly increased in the miR-502-3p agomiRs transfected cells, while it was decreased in miR-502-3p antagomiRs transfected cells compared with scramble control. (C) qRT-PCR analysis of GABRα1 mRNA in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 expression was decreased significantly in the miR-502-3p agomiRs transfected cells, while its levels were increased significantly in miR-502-3p antagomiRs transfected cells compared with scramble control. (D) Immunoblot analysis of GABRα1 protein in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 protein levels were decreased in the miR-502-3p agomiRs transfected cells. (E) Densitometry analysis of GABRα1 blot shows a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiRs show an increased level of GABRα1 protein. (F) Enzyme-linked immunosorbent assay showed a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs treated cells compared with scramble control and antagomiRs treated cells. For statistical analysis, one-way analysis of variance was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. GABRα1: Gamma-aminobutyric acid type A receptor subunit alpha1; miR: microRNA; qRT-PCR: quantitative reverse transcription-polymerase chain reaction.

    Techniques Used: Luciferase, Transfection, Construct, Activity Assay, Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Polymerase Chain Reaction


    Structured Review

    Bio-Tech Pharmacal Inc elisa kits
    Elisa Kits, supplied by Bio-Tech Pharmacal Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/Bio-Tech Pharmacal Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kits - by Bioz Stars, 2024-07
    86/100 stars

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    Danaher Inc tnf α elisa kit
    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), <t>and</t> <t>TNF-α</t> (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
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    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and <t>AST</t> (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using <t>ELISA.</t> C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
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    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and <t>AST</t> (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using <t>ELISA.</t> C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
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    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using <t>ELISA.</t> C The release of pro-inflammatory <t>cytokines</t> <t>IL-6</t> (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
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    Danaher Inc ifn γ elisa kit
    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), <t>IFN-γ</t> (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance
    Ifn γ Elisa Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cusabio diamine oxidase dao elisa kit
    The gut barrier function restored by SP is impaired by Veillonella parvula. A LPS levels (F (DFn, DFd) = 2.609 (5, 42), p = 0.0384) in mouse colon tissues were examined using <t>ELISA.</t> B <t>DAO</t> levels (F (DFn, DFd) = 1.373 (5, 42), p = 0.2537) in mouse serum were examined using ELISA. C Analysis of D-Lactate (F (DFn, DFd) = 2.825 (5, 42), p = 0.0275) in mouse serum by colorimetric method. D-F Immunofluorescence detection of Claudin-1 (F (DFn, DFd) = 1.295 (5, 42), p = 0.2843), Jam-a (F DFn, DFd) = 0.8971 (5, 42), p = 0.4919), and Occludin (F (DFn, DFd) = 0.5617 (5, 42), p = 0.7287) expression in mouse colon tissues. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (B, D, E, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (A, C), and Games-Howell's multiple comparisons test was used for post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance
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    MyBiosource Biotechnology immunosorbent assay elisa kit
    MiR-502-3p modulates GABRα1 levels. (A) Firefly/Renilla dual luciferase assay of hippocampal neurons (HT22) cells transfected with GABRα1 3’-UTR construct, control construct, miR-502-3p agomiRs and miR-502-3p antagomiRs. The luciferase activity was significantly reduced in the cells co-transfected with GABRα1 3’-UTR construct and miR-502-3p agomiRs relative to other groups. (B) qRT-PCR analysis of miR-502-3p in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. MiR-502-3p expression was significantly increased in the miR-502-3p agomiRs transfected cells, while it was decreased in miR-502-3p antagomiRs transfected cells compared with scramble control. (C) qRT-PCR analysis of GABRα1 mRNA in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 expression was decreased significantly in the miR-502-3p agomiRs transfected cells, while its levels were increased significantly in miR-502-3p antagomiRs transfected cells compared with scramble control. (D) Immunoblot analysis of GABRα1 protein in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 protein levels were decreased in the miR-502-3p agomiRs transfected cells. (E) Densitometry analysis of GABRα1 blot shows a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiRs show an increased level of GABRα1 protein. (F) <t>Enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> showed a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs treated cells compared with scramble control and antagomiRs treated cells. For statistical analysis, one-way analysis of variance was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. GABRα1: Gamma-aminobutyric acid type A receptor subunit alpha1; miR: microRNA; qRT-PCR: quantitative reverse transcription-polymerase chain reaction.
    Immunosorbent Assay Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiR-502-3p modulates GABRα1 levels. (A) Firefly/Renilla dual luciferase assay of hippocampal neurons (HT22) cells transfected with GABRα1 3’-UTR construct, control construct, miR-502-3p agomiRs and miR-502-3p antagomiRs. The luciferase activity was significantly reduced in the cells co-transfected with GABRα1 3’-UTR construct and miR-502-3p agomiRs relative to other groups. (B) qRT-PCR analysis of miR-502-3p in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. MiR-502-3p expression was significantly increased in the miR-502-3p agomiRs transfected cells, while it was decreased in miR-502-3p antagomiRs transfected cells compared with scramble control. (C) qRT-PCR analysis of GABRα1 mRNA in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 expression was decreased significantly in the miR-502-3p agomiRs transfected cells, while its levels were increased significantly in miR-502-3p antagomiRs transfected cells compared with scramble control. (D) Immunoblot analysis of GABRα1 protein in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 protein levels were decreased in the miR-502-3p agomiRs transfected cells. (E) Densitometry analysis of GABRα1 blot shows a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiRs show an increased level of GABRα1 protein. (F) <t>Enzyme-linked</t> <t>immunosorbent</t> <t>assay</t> showed a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs treated cells compared with scramble control and antagomiRs treated cells. For statistical analysis, one-way analysis of variance was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. GABRα1: Gamma-aminobutyric acid type A receptor subunit alpha1; miR: microRNA; qRT-PCR: quantitative reverse transcription-polymerase chain reaction.
    Elisa Kits, supplied by Bio-Tech Pharmacal Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Article Snippet: Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Article Snippet: Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Article Snippet: Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Deletion of Tlr4 suppresses Veillonella-induced liver injury in mice. A Detection of serum ALT (F (DFn, DFd) = 3.089 (2, 21), p = 0.0667), AST(F (DFn, DFd) = 3.367 (2, 21), p = 0.0539), IL-6 (F (DFn, DFd) = 1.747 (2, 21), p = 0.1987), TNF-α (F (DFn, DFd) = 3.430 (2, 21), p = 0.0514), DAO (F (DFn, DFd) = 0.2063 (2, 21), p = 0.8152), D-Lactate (F (DFn, DFd) = 0.7158 (2, 21), p = 0.5003) in mice. B The representative images of the livers. C The liver damage in mice was observed using HE staining. D Detection of fibrosis level (F (DFn, DFd) = 4.374 (2, 21), p = 0.0258) in mouse liver by Sirius red staining. E Immunohistochemical detection of Tlr4 (F (DFn, DFd) = 6.133 (2, 21), p = 0.0080) and Nlrp3 (F (DFn, DFd) = 6.078 (2, 21), p = 0.0083) expression in mouse liver tissues. F Immunofluorescence detection of gut barrier-related markers Claudin-1 (F (DFn, DFd) = 0.2207 (2, 21), p = 0.8038), Jam-a (F (DFn, DFd) = 0.03499 (2, 21), p = 0.9657), and Occludin (F (DFn, DFd) = 1.791, p = 0.1913) in mice. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (A, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsy the ANOVA test (D, E), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Staining, Immunohistochemical staining, Expressing, Immunofluorescence

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: HSC activation and HC pyroptosis are exacerbated by Veillonella parvula. A The effect of Veillonella-CM treatment on HSC viability (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385) was measured using CCK8. B The effect of Veillonella-CM treatment on the survival of HSC was examined using Calcein-AM/PI dual staining (F (DFn, DFd) = 5.124 (2, 2), p = 0.3266). C The effect of Veillonella-CM treatment on the protein expression of Collagen I (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571), Ctgf (F(DFn, DFd) = 4.333 (2, 2), p = 0.3750), α-SMA (F (DFn, DFd) = 10.86 (2, 2), p = 0.1687) in HSC was measured using a western blot. D The effect of Veillonella-CM treatment on the levels of cytokines IL-6 (F (DFn, DFd) = 4.180 (2, 2), p = 0.3861), IL-1β (F (DFn, DFd) = 3876 (2, 2), p = 0.0005), TNF-α (F (DFn, DFd) = 1.739 (2, 2), p = 0.7301) from HSC was examined using ELISA. E The effect of Veillonella-CM treatment on HC viability (F (DFn, DFd) = 7.896 (2, 2), p = 0.2248) was measured using CCK8. F The effect of Veillonella-CM treatment on the survival of HC (F (DFn, DFd) = 18.40 (2, 2), p = 0.1031) was examined using Calcein-AM/PI dual staining. G The effect of Veillonella-CM treatment on ALT (F (DFn, DFd) = 8.062 (2, 2), p = 0.2207) and AST (F (DFn, DFd) = 109.5 (2, 2), p = 0.0181) from HC. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (A, B, C, Da, Dc, E, F, Ga). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Db, Gb). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Activation Assay, Staining, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay

    Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Inhibition of Tlr4 attenuates the deteriorating effects of Veillonella-CM on HSC and HC. A The effect of Resatorvid treatment on the levels of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 3.992 (2, 2), p = 0.4006), IL-1β (F (DFn, DFd) = 2.540 (2, 2), p = 0.5649), TNF-α (F (DFn, DFd) = 77.46 (2, 2), p = 0.0255) released from HSC was examined using ELISA. B The effect of Resatorvid treatment on the survival of HSC (F (DFn, DFd) = 1.959 (2, 2), p = 0.6758) was examined using Calcein-AM/PI dual staining. C The protein expression of Tlr4 (F (DFn, DFd) = 2.714 (2, 2), p = 0.5385), Nlrp3 (F (DFn, DFd) = 3.857 (2, 2), p = 0.4118), Collagen I (F (DFn, DFd) = 7.947 (2, 2), p = 0.2235), Ctgf (F (DFn, DFd) = 1.714 (2, 2), p = 0.7368), α-SMA (F (DFn, DFd) = 3.250 (2, 2), p = 0.4706) in HSC was examined using western blot. D The protein expression of Tlr4 (F (DFn, DFd) = 4.000 (2, 2), p = 0.4000) and Nlrp3 (F (DFn, DFd) = 1.333 (2, 2), p = 0.8571) in HC was examined using western blot. E The effect of Resatorvid treatment on ALT (F (DFn, DFd) = 1.872 (2, 2), p = 0.6964) and AST (F (DFn, DFd) = 1.023 (2, 2), p = 0.9887) release from HC. F The effect of Resatorvid treatment on the survival of HC (F (DFn, DFd) = 1.590 (2, 2), p = 0.7723) was examined using Calcein-AM/PI dual staining. G Observation of morphology of HSC and HC under optical microscope. Data are expressed using dots and boxplots (n = 3). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Aa, Ab, B, C, D, E, F). Data that did not satisfy equal variances were analyzed by Welch’s t-test (Ac). *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing, Western Blot, Microscopy

    SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: SP improves LC in ConA-induced mice. A Experimental flow chart. B The levels of ALT (F (DFn, DFd) = 3.989 (3, 28), p = 0.0175) and AST (F (DFn, DFd) = 2.309 (3, 28), p = 0.0981) in mouse serum using ELISA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 4.631 (3, 28), p = 0.0094), IFN-γ (F (DFn, DFd) = 5.038 (3, 28), p = 0.0065), and TNF-α (F (DFn, DFd) = 1.701 (3, 28), p = 0.1895) in mouse serum using ELISA. D The representative images of the livers. E The liver damage in mice was observed using HE staining. F Detection of fibrosis level in mouse liver by Sirius red staining (F(DFn, DFd) = 5.595 (3, 28), p = 0.0039). G Positivity of Ki67 in liver parenchymal cells detected by immunohistochemistry (F (DFn, DFd), 3.352 (3, 28), p = 0.0330). Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (Bb, Cb), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (Ba, Ca, Cc, F, G), and Games-Howell's multiple comparisons test was used for post hoc testing. ***p < 0.001, ****p < 0.0001 indicates significance

    Article Snippet: Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemistry

    Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: Veillonella parvula antagonizes the ameliorative effect of SP on liver fibrosis. A Experimental flow chart. B Relative abundance of Veillonella (F(DFn, Dfd) = 9.904 (7, 7), p = 0.0072) detected by 16 s rRNA. C The release of pro-inflammatory cytokines IL-6 (F (DFn, DFd) = 1.133 (7, 7), p = 0.8734), IFN-γ (F (DFn, DFd) = 2.155 (7, 7), p = 0.3324), and TNF-α (F (DFn, DFd) = 6.216 (7, 7), p = 0.0278) in mouse serum using ELISA. D The levels of ALT (F(DFn, Dfd) = 1.188 (7, 7), p = 0.8260) and AST (F(DFn, Dfd) = 2.833 (7, 7), p = 0.1928) in mouse serum using ELISA. E The representative images of the livers. F Immunohistochemical detection of α-SMA expression in liver tissues of mice (F(DFn, Dfd) = 1.120 (7, 7), p = 0.8847). G Detection of fibrosis level (F(DFn, Dfd) = 1.021 (7, 7), p = 0.9784) in mouse liver by Sirius red staining. H The liver damage in mice was observed using HE staining. Data are expressed using dots and boxplots (n = 8). F test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by unpaired t-test (Ca, Cc, D, F, G). Data that did not satisfy equal variances were analyzed by Welch’s t-test (B, Cb). ****p < 0.0001 indicates significance

    Article Snippet: Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Expressing, Staining

    The gut barrier function restored by SP is impaired by Veillonella parvula. A LPS levels (F (DFn, DFd) = 2.609 (5, 42), p = 0.0384) in mouse colon tissues were examined using ELISA. B DAO levels (F (DFn, DFd) = 1.373 (5, 42), p = 0.2537) in mouse serum were examined using ELISA. C Analysis of D-Lactate (F (DFn, DFd) = 2.825 (5, 42), p = 0.0275) in mouse serum by colorimetric method. D-F Immunofluorescence detection of Claudin-1 (F (DFn, DFd) = 1.295 (5, 42), p = 0.2843), Jam-a (F DFn, DFd) = 0.8971 (5, 42), p = 0.4919), and Occludin (F (DFn, DFd) = 0.5617 (5, 42), p = 0.7287) expression in mouse colon tissues. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (B, D, E, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (A, C), and Games-Howell's multiple comparisons test was used for post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: Splenectomy ameliorates liver cirrhosis by restoring the gut microbiota balance

    doi: 10.1007/s00018-023-05055-5

    Figure Lengend Snippet: The gut barrier function restored by SP is impaired by Veillonella parvula. A LPS levels (F (DFn, DFd) = 2.609 (5, 42), p = 0.0384) in mouse colon tissues were examined using ELISA. B DAO levels (F (DFn, DFd) = 1.373 (5, 42), p = 0.2537) in mouse serum were examined using ELISA. C Analysis of D-Lactate (F (DFn, DFd) = 2.825 (5, 42), p = 0.0275) in mouse serum by colorimetric method. D-F Immunofluorescence detection of Claudin-1 (F (DFn, DFd) = 1.295 (5, 42), p = 0.2843), Jam-a (F DFn, DFd) = 0.8971 (5, 42), p = 0.4919), and Occludin (F (DFn, DFd) = 0.5617 (5, 42), p = 0.7287) expression in mouse colon tissues. Data are expressed using dots and boxplots (n = 8). Brown-Forsythe test [F (DFn, DFd)] was used for equal variances. Data satisfying equal variances were analyzed by one-way ANOVA test (B, D, E, F), and Tukey's multiple comparisons test was used for post hoc testing. Data that did not satisfy equal variances were analyzed by Brown-Forsythe ANOVA test (A, C), and Games-Howell's multiple comparisons test was used for post hoc testing. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 indicate significance

    Article Snippet: Biochemical testing Following the manufacturer’s protocols, the Alanine Aminotransferase (ALT/GPT) ELISA Kit (CSB-E16539m, Cusabio Biotech, Newark, DE, USA), Aspartate Aminotransferase (AST) ELISA kit (CSB-E12649m, Cusabio Biotech), IL-6 ELISA Kit (ab222503, Abcam, Cambridge, UK), IFN-γ ELISA Kit (ab282874, Abcam), TNF-α ELISA Kit (ab208348, Abcam), IL-1β ELISA Kit (ab197742, Abcam), Lipopolysaccharides (LPS) ELISA Kit (MU30451, Bioswamp, Wuhan, Hubei, China), diamine oxidase (DAO) ELISA Kit (CSB-E10090m, Cusabio Biotech), and D-Lactate Assay Kit (ab83429, Abcam) were applied for testing.

    Techniques: Enzyme-linked Immunosorbent Assay, Immunofluorescence, Expressing

    MiR-502-3p modulates GABRα1 levels. (A) Firefly/Renilla dual luciferase assay of hippocampal neurons (HT22) cells transfected with GABRα1 3’-UTR construct, control construct, miR-502-3p agomiRs and miR-502-3p antagomiRs. The luciferase activity was significantly reduced in the cells co-transfected with GABRα1 3’-UTR construct and miR-502-3p agomiRs relative to other groups. (B) qRT-PCR analysis of miR-502-3p in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. MiR-502-3p expression was significantly increased in the miR-502-3p agomiRs transfected cells, while it was decreased in miR-502-3p antagomiRs transfected cells compared with scramble control. (C) qRT-PCR analysis of GABRα1 mRNA in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 expression was decreased significantly in the miR-502-3p agomiRs transfected cells, while its levels were increased significantly in miR-502-3p antagomiRs transfected cells compared with scramble control. (D) Immunoblot analysis of GABRα1 protein in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 protein levels were decreased in the miR-502-3p agomiRs transfected cells. (E) Densitometry analysis of GABRα1 blot shows a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiRs show an increased level of GABRα1 protein. (F) Enzyme-linked immunosorbent assay showed a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs treated cells compared with scramble control and antagomiRs treated cells. For statistical analysis, one-way analysis of variance was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. GABRα1: Gamma-aminobutyric acid type A receptor subunit alpha1; miR: microRNA; qRT-PCR: quantitative reverse transcription-polymerase chain reaction.

    Journal: Neural Regeneration Research

    Article Title: MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

    doi: 10.4103/NRR.NRR-D-23-01064

    Figure Lengend Snippet: MiR-502-3p modulates GABRα1 levels. (A) Firefly/Renilla dual luciferase assay of hippocampal neurons (HT22) cells transfected with GABRα1 3’-UTR construct, control construct, miR-502-3p agomiRs and miR-502-3p antagomiRs. The luciferase activity was significantly reduced in the cells co-transfected with GABRα1 3’-UTR construct and miR-502-3p agomiRs relative to other groups. (B) qRT-PCR analysis of miR-502-3p in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. MiR-502-3p expression was significantly increased in the miR-502-3p agomiRs transfected cells, while it was decreased in miR-502-3p antagomiRs transfected cells compared with scramble control. (C) qRT-PCR analysis of GABRα1 mRNA in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 expression was decreased significantly in the miR-502-3p agomiRs transfected cells, while its levels were increased significantly in miR-502-3p antagomiRs transfected cells compared with scramble control. (D) Immunoblot analysis of GABRα1 protein in HT22 cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. GABRα1 protein levels were decreased in the miR-502-3p agomiRs transfected cells. (E) Densitometry analysis of GABRα1 blot shows a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiRs show an increased level of GABRα1 protein. (F) Enzyme-linked immunosorbent assay showed a significantly reduced level of GABRα1 protein in miR-502-3p agomiRs treated cells compared with scramble control and antagomiRs treated cells. For statistical analysis, one-way analysis of variance was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. GABRα1: Gamma-aminobutyric acid type A receptor subunit alpha1; miR: microRNA; qRT-PCR: quantitative reverse transcription-polymerase chain reaction.

    Article Snippet: The level of GABRα1 protein was checked in HT22 cell lysate using the mouse-GABRα1 enzyme-linked immunosorbent assay (ELISA) kit (Cat# MBS9339158, Mybiosource Inc., San Diego, CA, USA) as per manufacturer instruction.

    Techniques: Luciferase, Transfection, Construct, Activity Assay, Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Polymerase Chain Reaction