Structured Review

Enzo Biochem elisa kit
Effects of bv on the <t>LTB4</t> expression on the OVA challenged mice. LTB4 protein in lung homogenate was determined via <t>ELISA</t> kit. Normal control mice treated with PBS alone (NC), OVA-challenged mice treated with PBS (OVA), and OVA-challenged mice treated with an i.t. injection of bvPLA2 (10 µg/kg; OVA+PLA2). The statistical analyses were conducted by one-way ANOVA followed by Newman-Keuls multiple comparison test (* p
Elisa Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice"

Article Title: Protective Effects of Intratracheally-Administered Bee Venom Phospholipase A2 on Ovalbumin-Induced Allergic Asthma in Mice

Journal: Toxins

doi: 10.3390/toxins8100269

Effects of bv on the LTB4 expression on the OVA challenged mice. LTB4 protein in lung homogenate was determined via ELISA kit. Normal control mice treated with PBS alone (NC), OVA-challenged mice treated with PBS (OVA), and OVA-challenged mice treated with an i.t. injection of bvPLA2 (10 µg/kg; OVA+PLA2). The statistical analyses were conducted by one-way ANOVA followed by Newman-Keuls multiple comparison test (* p
Figure Legend Snippet: Effects of bv on the LTB4 expression on the OVA challenged mice. LTB4 protein in lung homogenate was determined via ELISA kit. Normal control mice treated with PBS alone (NC), OVA-challenged mice treated with PBS (OVA), and OVA-challenged mice treated with an i.t. injection of bvPLA2 (10 µg/kg; OVA+PLA2). The statistical analyses were conducted by one-way ANOVA followed by Newman-Keuls multiple comparison test (* p

Techniques Used: Expressing, Mouse Assay, Enzyme-linked Immunosorbent Assay, Injection

2) Product Images from "Nitric oxide enhances Th9 cell differentiation and airway inflammation"

Article Title: Nitric oxide enhances Th9 cell differentiation and airway inflammation

Journal: Nature communications

doi: 10.1038/ncomms5575

NO-induced up-regulation of Th9 polarization is p53-dependent. CD4 + T cells from WT and p53 −/− B6 mice were cultured under Th9 polarizing conditions ± NO. (a) The expression of p53 in WT Th9 cells on day 4 was analyzed by FACS. Concentrations of IL-9 (b) and IL-2 (c) in the culture supernatants of WT and p53 −/− cells on day 5 were determined by ELISA. (d, e) Percent of IL-9 + and pSTAT5 + T cells from WT and p53 −/− mice polarized under Th9 conditions was analyzed by FACS on day 4. Representative (f) and percent (g) of IL-9 + T cells from WT or p53 −/− mice cultured under Th9 conditions in the presence of NO with or without IL-2 are shown. (h) Expression of IRF4 in the Th9 cells from WT and p53 −/− mice polarized under Th9 conditions ± NO ± IL-2 was analyzed by FACS. Data are representative or pool of at least 3 experiments, mean ± SEM, n=3, *P
Figure Legend Snippet: NO-induced up-regulation of Th9 polarization is p53-dependent. CD4 + T cells from WT and p53 −/− B6 mice were cultured under Th9 polarizing conditions ± NO. (a) The expression of p53 in WT Th9 cells on day 4 was analyzed by FACS. Concentrations of IL-9 (b) and IL-2 (c) in the culture supernatants of WT and p53 −/− cells on day 5 were determined by ELISA. (d, e) Percent of IL-9 + and pSTAT5 + T cells from WT and p53 −/− mice polarized under Th9 conditions was analyzed by FACS on day 4. Representative (f) and percent (g) of IL-9 + T cells from WT or p53 −/− mice cultured under Th9 conditions in the presence of NO with or without IL-2 are shown. (h) Expression of IRF4 in the Th9 cells from WT and p53 −/− mice polarized under Th9 conditions ± NO ± IL-2 was analyzed by FACS. Data are representative or pool of at least 3 experiments, mean ± SEM, n=3, *P

Techniques Used: Mouse Assay, Cell Culture, Expressing, FACS, Enzyme-linked Immunosorbent Assay

NO enhances Th9 polarization via nitrosylation of MDM2-p53 complex. (a) BALB/c CD4 + T cells were polarized under Th9 conditions ± 100 μM NOC-18. p53/MDM2 complex in the cell extracts were determined by ELISA. (b) CD4 + T cells were polarized as above in the presence of cysteine (1 mM) or ascorbate (300 μM) for 24 h and the cell extracts analyzed for p53/MDM2 complex. Percentage of IL-9 + p53 + T cells on day 4 (c,), IL-9 + IL-2 + T cells on day 4 (d), IL-9 + pSTAT5 + T cells on day 5 (e), and IL-9 + IRF4 + T cells (f) on day 4 analyzed by FACS. Data are representative or pooled of at least 3 experiments; mean ± SEM, n=3-5, *P
Figure Legend Snippet: NO enhances Th9 polarization via nitrosylation of MDM2-p53 complex. (a) BALB/c CD4 + T cells were polarized under Th9 conditions ± 100 μM NOC-18. p53/MDM2 complex in the cell extracts were determined by ELISA. (b) CD4 + T cells were polarized as above in the presence of cysteine (1 mM) or ascorbate (300 μM) for 24 h and the cell extracts analyzed for p53/MDM2 complex. Percentage of IL-9 + p53 + T cells on day 4 (c,), IL-9 + IL-2 + T cells on day 4 (d), IL-9 + pSTAT5 + T cells on day 5 (e), and IL-9 + IRF4 + T cells (f) on day 4 analyzed by FACS. Data are representative or pooled of at least 3 experiments; mean ± SEM, n=3-5, *P

Techniques Used: Enzyme-linked Immunosorbent Assay, FACS

3) Product Images from "Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese"

Article Title: Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese

Journal: Frontiers in Endocrinology

doi: 10.3389/fendo.2018.00016

Secretion of HSP60 and HSP60 autoantibodies into blood. Circulating levels of (A) HSP60 protein and (B) HSP60 auto-Abs were measured by ELISA using plasma samples from obese people without (ND) and with diabetes (D) before and after a 3-month physical exercise intervention ( n = 43 for each group). The p -value was determined using the Mann–Whitney test for comparisons between the diabetes and non-diabetes groups and using a paired t -test for intragroup comparisons before and after exercise. * denotes p
Figure Legend Snippet: Secretion of HSP60 and HSP60 autoantibodies into blood. Circulating levels of (A) HSP60 protein and (B) HSP60 auto-Abs were measured by ELISA using plasma samples from obese people without (ND) and with diabetes (D) before and after a 3-month physical exercise intervention ( n = 43 for each group). The p -value was determined using the Mann–Whitney test for comparisons between the diabetes and non-diabetes groups and using a paired t -test for intragroup comparisons before and after exercise. * denotes p

Techniques Used: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin-Induced Apoptosis Mediated through the Akt-mTOR Pathway in Soleus Muscle
Article Snippet: .. Finally, stop solution was added, and the samples were analyzed at 450 nm using a plate reader. β -Catenin was analyzed using a commercially available ELISA kit (Enzo Life Sciences, cat. number ADI-900-135) following the manufacturer's instructions. ..

Article Title: Heat stress-induced memory impairment is associated with neuroinflammation in mice
Article Snippet: Materials Rabbit monoclonal anti-glial fibrillary acid protein (GFAP) was purchased from Millipore Bioscience Research (Bedford, MA, USA). .. Rabbit anti-c-fos and cortisol enzyme-linked immunosorbent assay (ELISA) kits were purchased from Enzo Life Sciences (Farmingdale, NY, USA). .. Goat anti-heat shock protein 70 (HSP70), anti-doublecortin (DCX), rabbit anti-nuclear factor (NF)-kB, anti-cyclooxygenase-2 (COX-2) and mouse anti-β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Article Title: Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages
Article Snippet: LPS (Escherichia Coli 055:B5) and berberine (purity > 98%) were from Sigma (St Louis, MO). .. TNF-α, IL-1β, and IL-6 ELISA kits were from Enzo Life Sciences (New York, NY). .. Antibodies against phospho-AMPK-α-Thr172 , AMPK, phospho-JNK (Thr183 /Tyr185 ), and phospho-p38 MAPK (Thr180 /Tyr182 ) were from Cell Signaling Technology (Danvers, MA).

Article Title: Translational Physiology: Analysis of short-term treatment with the phosphodiesterase type 5 inhibitor tadalafil on long bone development in young rats
Article Snippet: Primary chondrocytes were cultured to near confluence and then treated with 100 nM recombinant CNP (cat. no. N-8768, Sigma-Aldrich) in the absence or presence of 50 µM tadalafil (cat. no. SML-1877, Sigma-Aldrich) and 250 µM IBMX (cat. no. I-5879, Sigma-Aldrich). .. The cGMP concentrations in brain or cell culture were quantified by an ELISA immunoassay kit (cat. no. ADI-900-164, Enzo Life Sciences, Farmingdale, NY) following the manufacturer’s instructions. ..

Article Title: Coptidis Rhizoma Prevents Heat Stress-Induced Brain Damage and Cognitive Impairment in Mice
Article Snippet: Then, ether mixture was evaporated using nitrogen, after which protease activity was detected using a microplate reader (VERS Amax, Sunnyvale , CA, USA), with filters set at 570 nm excitation and 590 nm emission. .. The mouse TNF-α, IL-1β (Ray Biotech, Norcross, GA, USA), IL-9, IL-13, and PGE2 ELISA kits (Enzo, New York, NY, USA) were performed according to the manufacturer’s protocol. .. Briefly, the hypothalamic or hippocampal lysates were incubated with reaction buffer.

Article Title: Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese
Article Snippet: Quantification of Circulating Proteins by ELISA Plasma levels of HSP60 were measured by using a sandwich immunoassay EIA kit (ADI-EKS-600, Enzo, PA, USA). .. Plasma levels of anti-HSP60 IgG/A/M were measured using an ELISA kit (ADI-EKS-650, Enzo). ..

Article Title: Altered serum levels of IL-33 in patients with advanced systolic chronic heart failure: correlation with oxidative stress
Article Snippet: .. Concentrations of IL-33 and sST2 in serum were analyzed using commercially available enzyme-linked immunoassay (ELISA) kits (IL-33: Enzo, Farmingdale, New York, USA; sST2: Medical & Biological Laboratories, Naka-Ku, Nagoya, Japan). .. Given the possible role of sST2 in limiting IL-33 activity, we calculated the ratio of IL-33 to sST2 to approximately evaluate the possible bioactivity of circulating IL-33 [ ].

Article Title: GPR101 drives growth hormone hypersecretion and gigantism in mice via constitutive activation of Gs and Gq/11
Article Snippet: For the experiments on CRISPR/Cas9 HEK293 lines, ΔGs/olf , ΔGq/11 , ΔG12/13 , ΔGtot and parental cells were transiently co-transfected with GPR101 (or MOCK) and GloSensor cAMP biosensor and subjected to the same procedure. .. Measurement of cAMP levels by ELISA The Direct cAMP ELISA Kit (Enzo Life Sciences, Cat. No ADI-900-066) was used for the determination of cAMP levels in GH3 cells and mouse tissues. .. GH3 cells were grown in 24-well culture plates and transfected with increasing concentrations (0, 25, 50, 100, and 150 ng) of plasmid (MOCK or GPR101).

Cell Culture:

Article Title: Translational Physiology: Analysis of short-term treatment with the phosphodiesterase type 5 inhibitor tadalafil on long bone development in young rats
Article Snippet: Primary chondrocytes were cultured to near confluence and then treated with 100 nM recombinant CNP (cat. no. N-8768, Sigma-Aldrich) in the absence or presence of 50 µM tadalafil (cat. no. SML-1877, Sigma-Aldrich) and 250 µM IBMX (cat. no. I-5879, Sigma-Aldrich). .. The cGMP concentrations in brain or cell culture were quantified by an ELISA immunoassay kit (cat. no. ADI-900-164, Enzo Life Sciences, Farmingdale, NY) following the manufacturer’s instructions. ..

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  • 93
    Enzo Biochem hek293t cells
    NRH exposure altered NAD(H) and NADP(H) in <t>HEK293T</t> and HepG3 cells. Measurements of (A) NAD + , (B) NADH, (C) NADP + and (D) NADPH in HEK293T cells; (E) NAD + , (F) NADH, (G) NADP + and (H) NADPH in HepG3 cells exposed to media (control) and 100 μM NRH for 1, 4, and 24 h. Results are expressed as the average peak values relative to control ± SEM calculated from three biological replicates. Statistical significance: * P
    Hek293t Cells, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Enzo Biochem elisa kit
    Secretion of <t>HSP60</t> and HSP60 autoantibodies into blood. Circulating levels of (A) HSP60 protein and (B) HSP60 auto-Abs were measured by <t>ELISA</t> using plasma samples from obese people without (ND) and with diabetes (D) before and after a 3-month physical exercise intervention ( n = 43 for each group). The p -value was determined using the Mann–Whitney test for comparisons between the diabetes and non-diabetes groups and using a paired t -test for intragroup comparisons before and after exercise. * denotes p
    Elisa Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit/product/Enzo Biochem
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    95
    Enzo Biochem ho 1 elisa kit
    Validation of serum <t>HO-1</t> <t>ELISA.</t> (a) In a typical standard curve using modified ELISA, the correlation coefficient from 7 standard curves was 0.998 ± 0.002. The lower limit of detection was 0.038 ng/mL. (b) Comparison shows that the average serum HO-1 of 15 healthy samples was 17.2 ± 9.5 ng/mL by modified ELISA (all with measurable concentrations) and 2.1 ± 0.7 ng/mL by commercial ELISA (undetectable concentrations in 4 of 15 samples). ELISA, enzyme-linked immunosorbent assay; HO-1, hemeoxygenase-1.
    Ho 1 Elisa Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Enzo Biochem camp camp complete elisa kit
    Modulation of alanine metabolism in a <t>cAMP/CRP-dependent</t> manner. (A) Activity of ATPase of V. alginolyticus VA (ATCC 33787), Δ nqrA , and Δ nqrF strains in the presence or absence of 10 mM alanine. (B) ATP level in V. alginolyticus VA (ATCC 33787), Δ nqrA , and Δ nqrF in the presence or absence of alanine. (C) qRT-PCR for cyaA expression of VA, VA-R GEN , Δ nqrA , and Δ nqrF in the presence or absence of alanine. (D) <t>ELISA</t> for the cAMP level of VA, Δ nqrA , and Δ nqrF in the presence or absence of alanine. (E) qRT-PCR for crp expression of VA, VA-R GEN , Δ nqrA , and Δ nqrF in the presence or absence of alanine. (F) qRT-PCR for gene expression of alanine metabolism in Δ crp . (G) Integrative analysis of metabolite abundance (data from Fig. 2A ) and gene expression (data from panel F) in l- alanine metabolism of Δ crp . The blue and red colors denote downregulation and upregulation, respectively. Results in panels A to F are displayed as means ± SEM, and significant differences are identified (*, P
    Camp Camp Complete Elisa Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NRH exposure altered NAD(H) and NADP(H) in HEK293T and HepG3 cells. Measurements of (A) NAD + , (B) NADH, (C) NADP + and (D) NADPH in HEK293T cells; (E) NAD + , (F) NADH, (G) NADP + and (H) NADPH in HepG3 cells exposed to media (control) and 100 μM NRH for 1, 4, and 24 h. Results are expressed as the average peak values relative to control ± SEM calculated from three biological replicates. Statistical significance: * P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH exposure altered NAD(H) and NADP(H) in HEK293T and HepG3 cells. Measurements of (A) NAD + , (B) NADH, (C) NADP + and (D) NADPH in HEK293T cells; (E) NAD + , (F) NADH, (G) NADP + and (H) NADPH in HepG3 cells exposed to media (control) and 100 μM NRH for 1, 4, and 24 h. Results are expressed as the average peak values relative to control ± SEM calculated from three biological replicates. Statistical significance: * P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques:

    NRH exposure increased cellular reactive oxygen species (ROS) in HepG3 cells. ROS levels in HEK293T (A) and HepG3 (B) cells were assessed after 1, 4, and 24 h of NRH exposure using CM-H 2 DCFDA. Tert-butyl hydrogen peroxide (TBHP) at 250 μM exposure for 1 h was used as a positive control. Results are expressed as fluorescence intensity of treated samples relative to control of the average of the three biological replicates ± SEM. Statistical significance: * P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH exposure increased cellular reactive oxygen species (ROS) in HepG3 cells. ROS levels in HEK293T (A) and HepG3 (B) cells were assessed after 1, 4, and 24 h of NRH exposure using CM-H 2 DCFDA. Tert-butyl hydrogen peroxide (TBHP) at 250 μM exposure for 1 h was used as a positive control. Results are expressed as fluorescence intensity of treated samples relative to control of the average of the three biological replicates ± SEM. Statistical significance: * P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques: Positive Control, Fluorescence

    NRH exposure compromised mitochondrial function in HepG3 but not in HEK293T cells. The Seahorse Biosciences XF24 extracellular analyzer was used to measure mitochondrial stress test parameters. Representative OCR curves of untreated (white) and 100 μM NRH exposure for 1 h [ 39 ] and 24 h (red) in (A) HEK293T and (C) HepG3 cells. Mitochondrial respiratory function was assessed by adding oligomycin, FCCP and rotenone antimycin A and individual parameters for basal respiration, ATP production, proton leak, maximal respiration, spare respiratory capacity and non-mitochondrial respiration was measured in (B) HEK293T and (D) HepG3 cells. Results are expressed as mean OCR values relative to control ± the SEM of three independent experiments. Statistical significant *P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH exposure compromised mitochondrial function in HepG3 but not in HEK293T cells. The Seahorse Biosciences XF24 extracellular analyzer was used to measure mitochondrial stress test parameters. Representative OCR curves of untreated (white) and 100 μM NRH exposure for 1 h [ 39 ] and 24 h (red) in (A) HEK293T and (C) HepG3 cells. Mitochondrial respiratory function was assessed by adding oligomycin, FCCP and rotenone antimycin A and individual parameters for basal respiration, ATP production, proton leak, maximal respiration, spare respiratory capacity and non-mitochondrial respiration was measured in (B) HEK293T and (D) HepG3 cells. Results are expressed as mean OCR values relative to control ± the SEM of three independent experiments. Statistical significant *P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques:

    NRH increased mitochondria-derived superoxide formation in HepG3 but not in HEK293T cells. Cellular mitochondrial superoxide production was assessed by measuring the fluorescent intensity of MitoSOX Red in untreated and 100 μM NRH exposed (A) HEK293T and (B) HepG3 cells. Results are presented as mean fluorescence intensity relative to the control ± SEM of three independent experiments. Statistical significance: ** P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH increased mitochondria-derived superoxide formation in HepG3 but not in HEK293T cells. Cellular mitochondrial superoxide production was assessed by measuring the fluorescent intensity of MitoSOX Red in untreated and 100 μM NRH exposed (A) HEK293T and (B) HepG3 cells. Results are presented as mean fluorescence intensity relative to the control ± SEM of three independent experiments. Statistical significance: ** P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques: Derivative Assay, Fluorescence

    NRH exposure altered mitochondrial membrane potential (ΔΨ m ) in HepG3 but not in HEK293T cells. Mitochondrial membrane potential was assessed by measuring the mean fluorescent intensity of TMRE (plate assay) in NRH exposed (A) HEK293T and (B) HepG3 cells. FCCP uncouples the membrane potential resulting in loss of fluorescence signal and is thus used as a control. The graph represents the mean fluorescence intensity of three independent experiments normalized for cell number variations (see the Materials and Methods section) ± the SEM. Statistical significance: * P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH exposure altered mitochondrial membrane potential (ΔΨ m ) in HepG3 but not in HEK293T cells. Mitochondrial membrane potential was assessed by measuring the mean fluorescent intensity of TMRE (plate assay) in NRH exposed (A) HEK293T and (B) HepG3 cells. FCCP uncouples the membrane potential resulting in loss of fluorescence signal and is thus used as a control. The graph represents the mean fluorescence intensity of three independent experiments normalized for cell number variations (see the Materials and Methods section) ± the SEM. Statistical significance: * P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques: Fluorescence

    NRH exposure increased NADH/NADPH levels in HEK293T and HepG3. Autofluorescent images of endogenous NAD(P)H in untreated and 100 μM NRH exposed (A) HEK293T and (B) HepG3 cells. Quantification of fluorescent signal for untreated and 100 μM NRH treated (C) HEK293T, and (D) HepG3 cells. Results are expressed as the mean intensity of the fluorescent values relative to the control ± SEM calculated from three biological replicates. Statistically significance: * P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH exposure increased NADH/NADPH levels in HEK293T and HepG3. Autofluorescent images of endogenous NAD(P)H in untreated and 100 μM NRH exposed (A) HEK293T and (B) HepG3 cells. Quantification of fluorescent signal for untreated and 100 μM NRH treated (C) HEK293T, and (D) HepG3 cells. Results are expressed as the mean intensity of the fluorescent values relative to the control ± SEM calculated from three biological replicates. Statistically significance: * P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques:

    NRH exposure altered glutathione redox in HEK293T and HepG3 cells. Measurements of: (A) GSH, and (B) GSSG in HEK293T cells; (C) GSH, and (D) GSSG in HepG3 cells treated with NRH. Results are expressed as the mean luminescence values relative to the control ± SEM calculated from three biological replicates. Statistical significance: * P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH exposure altered glutathione redox in HEK293T and HepG3 cells. Measurements of: (A) GSH, and (B) GSSG in HEK293T cells; (C) GSH, and (D) GSSG in HepG3 cells treated with NRH. Results are expressed as the mean luminescence values relative to the control ± SEM calculated from three biological replicates. Statistical significance: * P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques:

    NRH induced PUMA and BAX-mediated apoptosis in HepG3 cells. Apoptosis in HEK293T and HepG3 cells were assessed using immunoblot after 24, 48, and 72 h of NRH exposure. Non-significant increases in the BAX and PUMA protein expression levels were observed in HEK293T cells (A). However, significantly increased expression of the apoptotic markers, BAX and PUMA were observed in HepG3 (D). The graph shows the protein expression levels relative to controls in HEK293T (B and C) and in HepG3 (E and F) cells. Results are expressed as the average of at least two biological replicates ± SEM. Statistical significance: * P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH induced PUMA and BAX-mediated apoptosis in HepG3 cells. Apoptosis in HEK293T and HepG3 cells were assessed using immunoblot after 24, 48, and 72 h of NRH exposure. Non-significant increases in the BAX and PUMA protein expression levels were observed in HEK293T cells (A). However, significantly increased expression of the apoptotic markers, BAX and PUMA were observed in HepG3 (D). The graph shows the protein expression levels relative to controls in HEK293T (B and C) and in HepG3 (E and F) cells. Results are expressed as the average of at least two biological replicates ± SEM. Statistical significance: * P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques: Expressing

    Mitochondrial DNA integrity of NRH-treated HEK293T and HepG3 cells. Electrophoresis and quantitative results from RT-PCR amplified products of mitochondrial DNA measured by QPCR assay (as described in the Materials and Methods section). Representative image of RT-PCR amplified product of mitochondrial DNA on agarose gel, stained with ethidium bromide in (A) HEK293T and (B) HepG3 cells exposed to 100 μM NRH for the indicated times. Quantification of relative amplification levels of mitochondrial DNA (mtDNA) in (C) HEK293T and (D) HepG3 cells from the agarose gel image using Image Lab software. Results are calculated by comparing the mtDNA amplification levels of the exposed samples with undamaged control ± SEM of three independent experiments. Statistical significance: * P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: Mitochondrial DNA integrity of NRH-treated HEK293T and HepG3 cells. Electrophoresis and quantitative results from RT-PCR amplified products of mitochondrial DNA measured by QPCR assay (as described in the Materials and Methods section). Representative image of RT-PCR amplified product of mitochondrial DNA on agarose gel, stained with ethidium bromide in (A) HEK293T and (B) HepG3 cells exposed to 100 μM NRH for the indicated times. Quantification of relative amplification levels of mitochondrial DNA (mtDNA) in (C) HEK293T and (D) HepG3 cells from the agarose gel image using Image Lab software. Results are calculated by comparing the mtDNA amplification levels of the exposed samples with undamaged control ± SEM of three independent experiments. Statistical significance: * P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques: Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Software

    NRH treatment dose-dependently decreased the number of surviving HepG3 cells. HEK293T (A) and HepG3 (B) cells were exposed to 100, 250, 500, 750 and 1000 μM of NRH for 24 h. After 24 h, NRH was removed and replaced with fresh medium, and cells were allowed to grow for another 48 h before performing CellTiter-Fluor TM Viability assay. Results are expressed as the mean fluorescence intensity to that of the control (% Viability) ± SEM. Statistical significance: * P

    Journal: PLoS ONE

    Article Title: Dihydronicotinamide riboside promotes cell-specific cytotoxicity by tipping the balance between metabolic regulation and oxidative stress

    doi: 10.1371/journal.pone.0242174

    Figure Lengend Snippet: NRH treatment dose-dependently decreased the number of surviving HepG3 cells. HEK293T (A) and HepG3 (B) cells were exposed to 100, 250, 500, 750 and 1000 μM of NRH for 24 h. After 24 h, NRH was removed and replaced with fresh medium, and cells were allowed to grow for another 48 h before performing CellTiter-Fluor TM Viability assay. Results are expressed as the mean fluorescence intensity to that of the control (% Viability) ± SEM. Statistical significance: * P

    Article Snippet: For HEK293T cells, poly D-lysine coated black plate was used to avoid washing off the cells during medium replacement.

    Techniques: Viability Assay, Fluorescence

    Secretion of HSP60 and HSP60 autoantibodies into blood. Circulating levels of (A) HSP60 protein and (B) HSP60 auto-Abs were measured by ELISA using plasma samples from obese people without (ND) and with diabetes (D) before and after a 3-month physical exercise intervention ( n = 43 for each group). The p -value was determined using the Mann–Whitney test for comparisons between the diabetes and non-diabetes groups and using a paired t -test for intragroup comparisons before and after exercise. * denotes p

    Journal: Frontiers in Endocrinology

    Article Title: Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese

    doi: 10.3389/fendo.2018.00016

    Figure Lengend Snippet: Secretion of HSP60 and HSP60 autoantibodies into blood. Circulating levels of (A) HSP60 protein and (B) HSP60 auto-Abs were measured by ELISA using plasma samples from obese people without (ND) and with diabetes (D) before and after a 3-month physical exercise intervention ( n = 43 for each group). The p -value was determined using the Mann–Whitney test for comparisons between the diabetes and non-diabetes groups and using a paired t -test for intragroup comparisons before and after exercise. * denotes p

    Article Snippet: Plasma levels of anti-HSP60 IgG/A/M were measured using an ELISA kit (ADI-EKS-650, Enzo).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Validation of serum HO-1 ELISA. (a) In a typical standard curve using modified ELISA, the correlation coefficient from 7 standard curves was 0.998 ± 0.002. The lower limit of detection was 0.038 ng/mL. (b) Comparison shows that the average serum HO-1 of 15 healthy samples was 17.2 ± 9.5 ng/mL by modified ELISA (all with measurable concentrations) and 2.1 ± 0.7 ng/mL by commercial ELISA (undetectable concentrations in 4 of 15 samples). ELISA, enzyme-linked immunosorbent assay; HO-1, hemeoxygenase-1.

    Journal: Canadian Respiratory Journal

    Article Title: ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome

    doi: 10.1155/2018/9627420

    Figure Lengend Snippet: Validation of serum HO-1 ELISA. (a) In a typical standard curve using modified ELISA, the correlation coefficient from 7 standard curves was 0.998 ± 0.002. The lower limit of detection was 0.038 ng/mL. (b) Comparison shows that the average serum HO-1 of 15 healthy samples was 17.2 ± 9.5 ng/mL by modified ELISA (all with measurable concentrations) and 2.1 ± 0.7 ng/mL by commercial ELISA (undetectable concentrations in 4 of 15 samples). ELISA, enzyme-linked immunosorbent assay; HO-1, hemeoxygenase-1.

    Article Snippet: Serum HO-1 ELISA Serum HO-1 concentrations were measured using the HO-1 ELISA kit (Enzo, Farmingdale, NY, USA) and the ImmunoSet™ HO-1 ELISA development set (Enzo, Farmingdale, NY, USA), according to the manufacturers' instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Modification

    Measurement of serum HO-1 in healthy volunteers using a commercial ELISA kit (HO-1 ELISA kit; Enzo, Farmingdale, NY, USA). Serum HO-1 at 1 : 1 dilution (×1) is plotted against the % recovery, which was calculated by spiking the samples with 3.13 ng/mL of the recombinant human HO-1 protein at a dilution of 1 : 2. ELISA, enzyme-linked immunosorbent assay; HO-1, hemeoxygenase-1.

    Journal: Canadian Respiratory Journal

    Article Title: ELISA Development for Serum Hemeoxygenase-1 and Its Application to Patients with Acute Respiratory Distress Syndrome

    doi: 10.1155/2018/9627420

    Figure Lengend Snippet: Measurement of serum HO-1 in healthy volunteers using a commercial ELISA kit (HO-1 ELISA kit; Enzo, Farmingdale, NY, USA). Serum HO-1 at 1 : 1 dilution (×1) is plotted against the % recovery, which was calculated by spiking the samples with 3.13 ng/mL of the recombinant human HO-1 protein at a dilution of 1 : 2. ELISA, enzyme-linked immunosorbent assay; HO-1, hemeoxygenase-1.

    Article Snippet: Serum HO-1 ELISA Serum HO-1 concentrations were measured using the HO-1 ELISA kit (Enzo, Farmingdale, NY, USA) and the ImmunoSet™ HO-1 ELISA development set (Enzo, Farmingdale, NY, USA), according to the manufacturers' instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

    Modulation of alanine metabolism in a cAMP/CRP-dependent manner. (A) Activity of ATPase of V. alginolyticus VA (ATCC 33787), Δ nqrA , and Δ nqrF strains in the presence or absence of 10 mM alanine. (B) ATP level in V. alginolyticus VA (ATCC 33787), Δ nqrA , and Δ nqrF in the presence or absence of alanine. (C) qRT-PCR for cyaA expression of VA, VA-R GEN , Δ nqrA , and Δ nqrF in the presence or absence of alanine. (D) ELISA for the cAMP level of VA, Δ nqrA , and Δ nqrF in the presence or absence of alanine. (E) qRT-PCR for crp expression of VA, VA-R GEN , Δ nqrA , and Δ nqrF in the presence or absence of alanine. (F) qRT-PCR for gene expression of alanine metabolism in Δ crp . (G) Integrative analysis of metabolite abundance (data from Fig. 2A ) and gene expression (data from panel F) in l- alanine metabolism of Δ crp . The blue and red colors denote downregulation and upregulation, respectively. Results in panels A to F are displayed as means ± SEM, and significant differences are identified (*, P

    Journal: mBio

    Article Title: Na+-NQR Confers Aminoglycoside Resistance via the Regulation of l-Alanine Metabolism

    doi: 10.1128/mBio.02086-20

    Figure Lengend Snippet: Modulation of alanine metabolism in a cAMP/CRP-dependent manner. (A) Activity of ATPase of V. alginolyticus VA (ATCC 33787), Δ nqrA , and Δ nqrF strains in the presence or absence of 10 mM alanine. (B) ATP level in V. alginolyticus VA (ATCC 33787), Δ nqrA , and Δ nqrF in the presence or absence of alanine. (C) qRT-PCR for cyaA expression of VA, VA-R GEN , Δ nqrA , and Δ nqrF in the presence or absence of alanine. (D) ELISA for the cAMP level of VA, Δ nqrA , and Δ nqrF in the presence or absence of alanine. (E) qRT-PCR for crp expression of VA, VA-R GEN , Δ nqrA , and Δ nqrF in the presence or absence of alanine. (F) qRT-PCR for gene expression of alanine metabolism in Δ crp . (G) Integrative analysis of metabolite abundance (data from Fig. 2A ) and gene expression (data from panel F) in l- alanine metabolism of Δ crp . The blue and red colors denote downregulation and upregulation, respectively. Results in panels A to F are displayed as means ± SEM, and significant differences are identified (*, P

    Article Snippet: Measurement of cAMP.cAMP Complete ELISA kit (Enzo Biochem Inc., New York, NY, USA) was used to assess the concentration of cAMP within bacterial cells.

    Techniques: Activity Assay, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay