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enzyme linked immunosorbent assay elisa kits  (Beijing Solarbio Science)
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
Elisa Assay Kits, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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enzyme linked immunosorbent assay elisa kits  (Cusabio)
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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enzyme linked immunosorbent assay elisa kits  (MyBiosource Biotechnology)
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) <t>ELISA</t> of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked <t>immunosorbent</t> assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.
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BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) ELISA of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked immunosorbent assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.

Journal: Neural Regeneration Research

Article Title: Changes in border-associated macrophages after stroke: Single-cell sequencing analysis

doi: 10.4103/NRR.NRR-D-24-01092

Figure Lengend Snippet: BAM depletion protects against acute stroke. (A) Flowchart of the experimental design. (B) Cerebral blood perfusion images of the brain within 60 seconds in the control and treated groups 24 hours after surgery. Less blue color in the right zone of brain in the treated group indicates better blood flow compared with the control group. (C) TTC staining images of brain from the control and treated groups 24 hours after surgery. Less white color was observed in the right brain zone of the treated group compared with the control group. Scale bars: 1 cm. (D) Infarct volume was quantified by TTC staining. (E) Neurological scores were assessed at 24 hours in the control and treated groups. (F) TNF-α, STAT3, and F4/80 immunopositivity in the control and treated groups 24 hours after surgery. (G) ELISA of TNF-α and STAT3 plasma levels 3 days after surgery. Data are expressed as mean ± SD ( n = 4 in both groups). * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed Student’s t -test). BAM: Border-associated macrophage; CBF: cerebral blood flow; ELISA: enzyme linked immunosorbent assay; HE: hematoxylin-eosin staining; IF: immunofluorescence staining; STAT3: signal transducer and activator of transcription 3; TNF: tumor necrosis factor; TTC: 2,3, 5-triphenyltetrazolium chloride.

Article Snippet: The plasma levels of TNF-α and STAT3 in both the control and treated groups were detected using enzyme-linked immunosorbent assay (ELISA) kits (Solarbio) according to the manufacturer’s instructions.

Techniques: Control, Staining, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Two Tailed Test, Immunofluorescence