elisa kit  (Abcam)

 
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    Corticosterone ELISA kit
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    ab108821
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    Abcam elisa kit
    161-MAP active vaccination reduces <t>EMMPRIN</t> expression in the colon. (A) Representative images of colon sections stained for EMMPRIN and their quantification using the H-score ( n = 5 per group). Scale bar is 25 μm (B) Representative image of fluorescently labeled EMMPRIN (red) and macrophages (green). Scale bar is 100 μm. (C) Determination of EMMPRIN concentrations in colon lysates ( n = 9–10 per group), and in serum samples ( n = 8–9 per group) by <t>ELISA.</t>

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    1) Product Images from "Active Vaccination With EMMPRIN-Derived Multiple Antigenic Peptide (161-MAP) Reduces Angiogenesis in a Dextran Sodium Sulfate (DSS)-Induced Colitis Model"

    Article Title: Active Vaccination With EMMPRIN-Derived Multiple Antigenic Peptide (161-MAP) Reduces Angiogenesis in a Dextran Sodium Sulfate (DSS)-Induced Colitis Model

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.02919

    161-MAP active vaccination reduces EMMPRIN expression in the colon. (A) Representative images of colon sections stained for EMMPRIN and their quantification using the H-score ( n = 5 per group). Scale bar is 25 μm (B) Representative image of fluorescently labeled EMMPRIN (red) and macrophages (green). Scale bar is 100 μm. (C) Determination of EMMPRIN concentrations in colon lysates ( n = 9–10 per group), and in serum samples ( n = 8–9 per group) by ELISA.
    Figure Legend Snippet: 161-MAP active vaccination reduces EMMPRIN expression in the colon. (A) Representative images of colon sections stained for EMMPRIN and their quantification using the H-score ( n = 5 per group). Scale bar is 25 μm (B) Representative image of fluorescently labeled EMMPRIN (red) and macrophages (green). Scale bar is 100 μm. (C) Determination of EMMPRIN concentrations in colon lysates ( n = 9–10 per group), and in serum samples ( n = 8–9 per group) by ELISA.

    Techniques Used: Expressing, Staining, Labeling, Enzyme-linked Immunosorbent Assay

    161-MAP active vaccination reduces angiogenesis. (A) Colon sections were stained for CD31 and the vessel surface area was calculated ( n = 4 per group). Scale bar is 100 μm. (B) Concentrations of VEGF and MMP-9 were determined in serum sample by ELISA, and in the colon lysates, normalized to the total protein amounts ( n = 9 per group). (C) Wound scratch assay: colon lysates (25 μg of total protein) were diluted (1:4) and applied onto a confluent layer of the mouse bEND3 endothelial cells (10 5 cells/ 96-plate well) that was scratched with a toothpick. Images were acquired at the beginning of the experiment (T0) and at the end after 24h (T24). The migration area was calculated by subtracting the area of the wound at T24, after endothelial cell migrated and partially closed the wound, from the area of the wound at T0. An EMMPRIN specific blocking antibody (161-pAb) was added to some of the wells as indicated. ( n = 9–10 for the male mice, n = 8 for the female mice). Magnification is x4.
    Figure Legend Snippet: 161-MAP active vaccination reduces angiogenesis. (A) Colon sections were stained for CD31 and the vessel surface area was calculated ( n = 4 per group). Scale bar is 100 μm. (B) Concentrations of VEGF and MMP-9 were determined in serum sample by ELISA, and in the colon lysates, normalized to the total protein amounts ( n = 9 per group). (C) Wound scratch assay: colon lysates (25 μg of total protein) were diluted (1:4) and applied onto a confluent layer of the mouse bEND3 endothelial cells (10 5 cells/ 96-plate well) that was scratched with a toothpick. Images were acquired at the beginning of the experiment (T0) and at the end after 24h (T24). The migration area was calculated by subtracting the area of the wound at T24, after endothelial cell migrated and partially closed the wound, from the area of the wound at T0. An EMMPRIN specific blocking antibody (161-pAb) was added to some of the wells as indicated. ( n = 9–10 for the male mice, n = 8 for the female mice). Magnification is x4.

    Techniques Used: Staining, Enzyme-linked Immunosorbent Assay, Wound Healing Assay, Migration, Blocking Assay, Mouse Assay

    2) Product Images from "Tumor Necrosis Factor-? and Muc2 Mucin Play Major Roles in Disease Onset and Progression in Dextran Sodium Sulphate-Induced Colitis"

    Article Title: Tumor Necrosis Factor-? and Muc2 Mucin Play Major Roles in Disease Onset and Progression in Dextran Sodium Sulphate-Induced Colitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0025058

    The effect of TNF-α neutralizing antibody treatment on disease onset and progression in DSS induced colitis. A : Comparison of disease activity index (DAI) on day 2, 5 and 9 of DSS treatment in animals that received 5% DSS in drinking water alone (black circles) or with TNF-α neutralizing antibody (grey diamonds). B : Body weight change plotted for control (asterisks), DSS only (black circles) or DSS + TNF-α neutralizing antibody treatment (grey diamonds). Data represents the means ± SEM from 6 animals per day. C : TNF-α protein secretion measured by ELISA on days 2, 5 and 9 of DSS treatment in animals that received 5% DSS in drinking water alone (black circles) or with TNF-α neutralizing antibody (grey diamonds). Data shown are the means ± SEM of 6-animals/day. * P
    Figure Legend Snippet: The effect of TNF-α neutralizing antibody treatment on disease onset and progression in DSS induced colitis. A : Comparison of disease activity index (DAI) on day 2, 5 and 9 of DSS treatment in animals that received 5% DSS in drinking water alone (black circles) or with TNF-α neutralizing antibody (grey diamonds). B : Body weight change plotted for control (asterisks), DSS only (black circles) or DSS + TNF-α neutralizing antibody treatment (grey diamonds). Data represents the means ± SEM from 6 animals per day. C : TNF-α protein secretion measured by ELISA on days 2, 5 and 9 of DSS treatment in animals that received 5% DSS in drinking water alone (black circles) or with TNF-α neutralizing antibody (grey diamonds). Data shown are the means ± SEM of 6-animals/day. * P

    Techniques Used: Activity Assay, Enzyme-linked Immunosorbent Assay

    3) Product Images from "Biosimilar Gene Therapy: Investigational Assessment of Secukinumab Gene Therapy"

    Article Title: Biosimilar Gene Therapy: Investigational Assessment of Secukinumab Gene Therapy

    Journal: Cell Journal (Yakhteh)

    doi: 10.22074/cellj.2020.6309

    Cell viability MTT assay and secukinumab bioassay. A. MTT assay results for CMSCs before and after transduction with secukinumab transfer vector. As shown in the figure, transduction with an MOI of 1,5, or10 has no significant effect on CMSC viability and B. ELISA test results of IL-6 on human primary dermal fibroblast in increasing concentrations of secukinumab in the presence of recombinant human Interleukin-17A (15 ng/ml). After 72 hours reduced IL-6 secretions from fibroblasts confirmed the inhibitory activity of secukinumab on human IL-17. CMSCs; Human chorionic derived mesenchymal stem cells, MOI; Multiplicity of infection, and OD; Optical density.
    Figure Legend Snippet: Cell viability MTT assay and secukinumab bioassay. A. MTT assay results for CMSCs before and after transduction with secukinumab transfer vector. As shown in the figure, transduction with an MOI of 1,5, or10 has no significant effect on CMSC viability and B. ELISA test results of IL-6 on human primary dermal fibroblast in increasing concentrations of secukinumab in the presence of recombinant human Interleukin-17A (15 ng/ml). After 72 hours reduced IL-6 secretions from fibroblasts confirmed the inhibitory activity of secukinumab on human IL-17. CMSCs; Human chorionic derived mesenchymal stem cells, MOI; Multiplicity of infection, and OD; Optical density.

    Techniques Used: MTT Assay, Transduction, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Recombinant, Activity Assay, Derivative Assay, Infection

    Secukinumab in vitro and in vivo ELISA. A. In vitro ELISA tests of secukinumab production from transducted CHO cells and CMSCs with the secukinumab transfer vector. Stem cells showed slightly higher mAbs production in comparison with CHO cells. Sampling was done 4 times a month and B. Rat serum ELISA results with five blood samples taken after treatment during the two-month duration. Secukinumab concentration resulting from in vivo lentivirus (orange) gene therapy is higher than CMSC-mediated ex vivo (blue) gene therapy. ELISA; Enzyme-linked immunosorbent assay, CHO; Chinese hamster ovary, and CMSCs; Human chorionic derived mesenchymal stem cells.
    Figure Legend Snippet: Secukinumab in vitro and in vivo ELISA. A. In vitro ELISA tests of secukinumab production from transducted CHO cells and CMSCs with the secukinumab transfer vector. Stem cells showed slightly higher mAbs production in comparison with CHO cells. Sampling was done 4 times a month and B. Rat serum ELISA results with five blood samples taken after treatment during the two-month duration. Secukinumab concentration resulting from in vivo lentivirus (orange) gene therapy is higher than CMSC-mediated ex vivo (blue) gene therapy. ELISA; Enzyme-linked immunosorbent assay, CHO; Chinese hamster ovary, and CMSCs; Human chorionic derived mesenchymal stem cells.

    Techniques Used: In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Sampling, Concentration Assay, Ex Vivo, Derivative Assay

    4) Product Images from "Overexpression of Toll-like Receptor 4-linked Mitogen-activated Protein Kinase Signaling Contributes to Internalization of Escherichia coli in Sheep"

    Article Title: Overexpression of Toll-like Receptor 4-linked Mitogen-activated Protein Kinase Signaling Contributes to Internalization of Escherichia coli in Sheep

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.25275

    qRT-PCR and ELISA testing of expression in sheep monocytes with LPS stimulation. A, B and C show the expression of TLR4 , Myd88 and NF-κB , at 0min, 5 min, 30 min and 60 min after LPS (100 ng/mL) stimulation, respectively. D, E and F show the phosphorylation levels of p38 , JNK , and ERK signaling at 0min, 5 min, 30 min and 60 min after LPS (100 ng/mL) stimulation, respectively. Tg: transgenic sheep; NTg: non-transgenic sheep. All data are presented as the mean ± SEM from three experiments, *P
    Figure Legend Snippet: qRT-PCR and ELISA testing of expression in sheep monocytes with LPS stimulation. A, B and C show the expression of TLR4 , Myd88 and NF-κB , at 0min, 5 min, 30 min and 60 min after LPS (100 ng/mL) stimulation, respectively. D, E and F show the phosphorylation levels of p38 , JNK , and ERK signaling at 0min, 5 min, 30 min and 60 min after LPS (100 ng/mL) stimulation, respectively. Tg: transgenic sheep; NTg: non-transgenic sheep. All data are presented as the mean ± SEM from three experiments, *P

    Techniques Used: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing, Transgenic Assay

    5) Product Images from "NLRP3 inflammasome activation contributes to Listeria monocytogenes-induced animal pregnancy failure"

    Article Title: NLRP3 inflammasome activation contributes to Listeria monocytogenes-induced animal pregnancy failure

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-016-0655-2

    LM activates NLRP3 inflammasome by LLO in mouse B6 macrophages ( a ) IL-1β levels in the culture medium of mouse B6 cells and its derived gene knock-out cells infected with wild-type or Δ hly LM was measured by ELISA. b IL-1β levels in the culture medium of wild-type and NLRP3 −/− B6 cells infected with wild-type LM and Δ hly LM, respectively. c and ( d ) Western blot for detection of activated caspase-1 (P10) in wild-type and NLRP3 −/− B6 cells infected with LM or Δ hly LM. LPS + ATP as a positive control of NLRP3 inflammasome activation. LPS + dA:dT (1.5 μg/10 6 cells) as a positive control of AIM2 inflammasome activation. The data in ( a ) and ( b ) are shown as means ± standard deviation from three independent experiments. * p
    Figure Legend Snippet: LM activates NLRP3 inflammasome by LLO in mouse B6 macrophages ( a ) IL-1β levels in the culture medium of mouse B6 cells and its derived gene knock-out cells infected with wild-type or Δ hly LM was measured by ELISA. b IL-1β levels in the culture medium of wild-type and NLRP3 −/− B6 cells infected with wild-type LM and Δ hly LM, respectively. c and ( d ) Western blot for detection of activated caspase-1 (P10) in wild-type and NLRP3 −/− B6 cells infected with LM or Δ hly LM. LPS + ATP as a positive control of NLRP3 inflammasome activation. LPS + dA:dT (1.5 μg/10 6 cells) as a positive control of AIM2 inflammasome activation. The data in ( a ) and ( b ) are shown as means ± standard deviation from three independent experiments. * p

    Techniques Used: Derivative Assay, Knock-Out, Infection, Enzyme-linked Immunosorbent Assay, Western Blot, Positive Control, Activation Assay, Standard Deviation

    6) Product Images from "Pomegranate prevents binge alcohol-induced gut leakiness and hepatic inflammation by suppressing oxidative and nitrative stress"

    Article Title: Pomegranate prevents binge alcohol-induced gut leakiness and hepatic inflammation by suppressing oxidative and nitrative stress

    Journal: Redox Biology

    doi: 10.1016/j.redox.2018.07.012

    POM prevented increased hepatic inflammation marker proteins in binge alcohol exposed rats. (A and B) ELISA results for TNF-α and MCP-1 in the liver lysates from control (CON), pomegranate (POM), ethanol (EtOH), or POM+EtOH-exposed rats are presented. (C) The levels of hepatic TLR4 and inflammation-associated proteins (TNF-α, IL-1β, and MCP-1) in the indicated groups are presented. Significant difference between various treatments was determined by one-way ANOVA. Labeled characters without a common letter represent significant differences from the other group(s).
    Figure Legend Snippet: POM prevented increased hepatic inflammation marker proteins in binge alcohol exposed rats. (A and B) ELISA results for TNF-α and MCP-1 in the liver lysates from control (CON), pomegranate (POM), ethanol (EtOH), or POM+EtOH-exposed rats are presented. (C) The levels of hepatic TLR4 and inflammation-associated proteins (TNF-α, IL-1β, and MCP-1) in the indicated groups are presented. Significant difference between various treatments was determined by one-way ANOVA. Labeled characters without a common letter represent significant differences from the other group(s).

    Techniques Used: Marker, Enzyme-linked Immunosorbent Assay, Labeling

    7) Product Images from "A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage"

    Article Title: A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage

    Journal: JHEP Reports

    doi: 10.1016/j.jhepr.2019.100064

    CM-101 suppressed CCL24-induced activation of HSCs in vitro . (A) Representative images and quantification of HSC (LX-2 cell line) scratch assay. Images of scratched wells were taken at time 0 h, 24 h and 48 h. Percentage closure of scratched area was evaluated for control group or cells treated with CCL24 (25 ng/ml) with or without CM-101 (5 μg/ml). (B) α-SMA expression measured by FACS following treatment with CCL24, with or without CM-101 (C) Pro-collagen I alpha secretion was quantified with a commercial ELISA kit, following activation of HSCs with CCL24 (100 ng/ml, 48 h incubation) with or without CM-101. Student's t test; * p ≤0.01, ** p ≤0.005, *** p ≤0.001, each graph represents an average of 3 different experiments. HSCs, hepatic stellate cells; MFI, median fluorescence intensity.
    Figure Legend Snippet: CM-101 suppressed CCL24-induced activation of HSCs in vitro . (A) Representative images and quantification of HSC (LX-2 cell line) scratch assay. Images of scratched wells were taken at time 0 h, 24 h and 48 h. Percentage closure of scratched area was evaluated for control group or cells treated with CCL24 (25 ng/ml) with or without CM-101 (5 μg/ml). (B) α-SMA expression measured by FACS following treatment with CCL24, with or without CM-101 (C) Pro-collagen I alpha secretion was quantified with a commercial ELISA kit, following activation of HSCs with CCL24 (100 ng/ml, 48 h incubation) with or without CM-101. Student's t test; * p ≤0.01, ** p ≤0.005, *** p ≤0.001, each graph represents an average of 3 different experiments. HSCs, hepatic stellate cells; MFI, median fluorescence intensity.

    Techniques Used: Activation Assay, In Vitro, Wound Healing Assay, Expressing, FACS, Enzyme-linked Immunosorbent Assay, Incubation, Fluorescence

    Anti-CCL24 antibody (CM-101 (D8)) reduced NASH-related pathologies in the MCD-diet mouse model (treatment mode). (A) Representative H E stained histological liver samples. Healthy liver from chow diet-fed mice (upper panels), MCD-fed WT mice (middle panels) and MCD + 5 mg/kg CM-101 (D8) treated group (bottom panels). (B) Histological scoring, comparisons of the 2 groups fed with the MCD diet (MCD diet and MCD diet + 5 mg/kg CM-101 (D8), n = 8). (C-E) AST, ALT and bilirubin levels in the MCD-induced NASH model compared to MCD + 5 mg/kg CM-101 (D8) treated group. (F) CCL24 levels in the liver measured by ELISA using total protein liver lysates from chow diet-fed mice, MCD-fed WT mice and MCD + 5 mg/kg CM-101 (D8) treated mice. Results are presented as average ±SE; Student's t test; * p ≤0.05, ** p ≤0.01, *** p ≤0.001. ALT, alanine aminotransferase; AST, aspartate aminotransferase; MCD, methionine-choline deficient; NAS, non-alcoholic fatty liver disease activity score; WT, wild-type.
    Figure Legend Snippet: Anti-CCL24 antibody (CM-101 (D8)) reduced NASH-related pathologies in the MCD-diet mouse model (treatment mode). (A) Representative H E stained histological liver samples. Healthy liver from chow diet-fed mice (upper panels), MCD-fed WT mice (middle panels) and MCD + 5 mg/kg CM-101 (D8) treated group (bottom panels). (B) Histological scoring, comparisons of the 2 groups fed with the MCD diet (MCD diet and MCD diet + 5 mg/kg CM-101 (D8), n = 8). (C-E) AST, ALT and bilirubin levels in the MCD-induced NASH model compared to MCD + 5 mg/kg CM-101 (D8) treated group. (F) CCL24 levels in the liver measured by ELISA using total protein liver lysates from chow diet-fed mice, MCD-fed WT mice and MCD + 5 mg/kg CM-101 (D8) treated mice. Results are presented as average ±SE; Student's t test; * p ≤0.05, ** p ≤0.01, *** p ≤0.001. ALT, alanine aminotransferase; AST, aspartate aminotransferase; MCD, methionine-choline deficient; NAS, non-alcoholic fatty liver disease activity score; WT, wild-type.

    Techniques Used: Staining, Mouse Assay, AST Assay, Enzyme-linked Immunosorbent Assay, Activity Assay

    8) Product Images from "Neuroprotective Effects of Ginsenoside-Rg1 Against Depression-Like Behaviors via Suppressing Glial Activation, Synaptic Deficits, and Neuronal Apoptosis in Rats"

    Article Title: Neuroprotective Effects of Ginsenoside-Rg1 Against Depression-Like Behaviors via Suppressing Glial Activation, Synaptic Deficits, and Neuronal Apoptosis in Rats

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.02889

    Ginsenoside-Rg1 attenuated microglial activation and inflammatory cytokine expressions induced by CUMS-exposure. (A) CUMS exposure significantly increased the number of Iba1 positive microglial cells within the vmPFC. Microglial cells retracted their processes and assumed a rounded morphology indicative of activated microglia. All of these CUMS-induced effects were significantly attenuated by ginsenoside-Rg1 pretreatment. (B) Real-time quantitative PCR assays showed that CUMS exposure increased IL-1β, IFN-γ, and TNF-α mRNA expression within the vmPFC, effects which were suppressed by ginsenoside-Rg1. (C) ELISA assays showed that CUMS exposure increased IL-1β, IFN-γ, and TNF-α protein expressions within the vmPFC, effects which were ameliorated by ginsenoside-Rg1. Data were presented as the means ± SEM ( N = 6). *** P
    Figure Legend Snippet: Ginsenoside-Rg1 attenuated microglial activation and inflammatory cytokine expressions induced by CUMS-exposure. (A) CUMS exposure significantly increased the number of Iba1 positive microglial cells within the vmPFC. Microglial cells retracted their processes and assumed a rounded morphology indicative of activated microglia. All of these CUMS-induced effects were significantly attenuated by ginsenoside-Rg1 pretreatment. (B) Real-time quantitative PCR assays showed that CUMS exposure increased IL-1β, IFN-γ, and TNF-α mRNA expression within the vmPFC, effects which were suppressed by ginsenoside-Rg1. (C) ELISA assays showed that CUMS exposure increased IL-1β, IFN-γ, and TNF-α protein expressions within the vmPFC, effects which were ameliorated by ginsenoside-Rg1. Data were presented as the means ± SEM ( N = 6). *** P

    Techniques Used: Activation Assay, Real-time Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay

    9) Product Images from "Differentiation of umbilical cord mesenchymal stem cells into hepatocytes in comparison with bone marrow mesenchymal stem cells"

    Article Title: Differentiation of umbilical cord mesenchymal stem cells into hepatocytes in comparison with bone marrow mesenchymal stem cells

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2018.9181

    Detection of secreted albumin and blood urea nitrogen by ELISA. The ALB and BUN concentration of UC-MSCs and BM-MSCs culture supernatants collected on weeks 1, 2, 3, 4 and 5 following hepatic differentiation were measured. The medium of hepatocyte was measured as a control. Each bar represents the mean ± standard deviation (n=3). *P
    Figure Legend Snippet: Detection of secreted albumin and blood urea nitrogen by ELISA. The ALB and BUN concentration of UC-MSCs and BM-MSCs culture supernatants collected on weeks 1, 2, 3, 4 and 5 following hepatic differentiation were measured. The medium of hepatocyte was measured as a control. Each bar represents the mean ± standard deviation (n=3). *P

    Techniques Used: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Sex-Specific Differences in Rodents Following a Single Primary Blast Exposure: Focus on the Monoamine and Galanin Systems
    Article Snippet: Progesterone (PROG) and estradiol (E2) levels were measured using Rat PROG ELISA Kit and Rat E2 ELISA Kit (both from CUSABIO, Nordic BioSite, Stockholm, Sweden) at 0.2 ng/ml and 40 pg/ml sensitivity, respectively. .. Corticosterone (CORT) levels were measured using Abcam's Corticosterone ELISA Kit (BioNordika, Stockholm, Sweden) at a sensitivity of 0.3 ng/ml. .. Statistical Analysis All statistical analyses were performed using GraphPad Prism version 6 (GraphPad Software, CA).

    Article Title: Social support rescues acute stress-induced cognitive impairments by modulating ERK1/2 phosphorylation in adolescent mice
    Article Snippet: .. An Enzyme-Linked Immunosorbent Assay (ELISA) was performed to measure the level of corticosterone following the Corticosterone ELISA kit (CAT #. ab108821) from Abcam (Cambridge, United Kingdom). ..

    Article Title: Artificial Light at Night of Different Spectral Compositions Differentially Affects Tumor Growth in Mice: Interaction With Melatonin and Epigenetic Pathways
    Article Snippet: Concentrations (ng/mL) of 6-SMT were spectrophotometrically determined by ELISA microplate reader at 450 nm with reference wavelength of 650 nm (PowerWave HT; Biotek, Winooski, Vermont) and analyzed by Gen5 Data Analysis Software (version 2; Biotek). .. Urinary corticosterone concentration was determined using an ELISA kit (ab108821; Abcam, Cambridge, United Kingdom) with 96% recovery for corticosterone, 0.3 ng/mL sensitivity, and 5.0% CV intra-assay variation. ..

    Article Title: Examining the central effects of chronic stressful social isolation on rats
    Article Snippet: After serum was collected in Eppendorf tubes, samples were stored at -80˚C. .. Serum corticosterone levels were analyzed using an ELISA kit in accordance with the manufacturer's protocol (Abcam; cat. no. ab108821). .. The absorbance of the standards and samples was measured using a BioTek® Synergy™ HT microplate reader (Bio-Tek Instruments, Inc.) at a wavelength of 450 nm.

    Article Title: Neonatal repetitive pain in rats leads to impaired spatial learning and dysregulated hypothalamic-pituitary-adrenal axis function in later life
    Article Snippet: .. Serum corticosterone was measured using an enzyme-linked immunosorbent assay (ELISA) kit (ab108821, Abcam, Cambridge, UK), for which the sensitivity limit was 0.3 ng/ml, the intra-assay coefficient of variation was 5.0%, and the inter-assay coefficient was 7.2%. .. Serum ACTH was analyzed by electrochemiluminescence immunoassay (ECLIA) using a Roche Cobas® 6000 Analyzer (Roche Diagnostics GmbH, Mannheim, Germany).

    Article Title: Benefits of A Three-Day Bamboo Forest Therapy Session on the Psychophysiology and Immune System Responses of Male College Students
    Article Snippet: Anti-CD3, anti-CD16, and anti-CD56 were purchased from Biolegend (San Diego, CA, USA). .. The Human CORT ELISA KIT (XL-Eh0551), Human GNLY ELISA KIT (XL-Eh1850), Human Gzms-A ELISA KIT (XL-Eh1375), Human Gzms-B ELISA KIT (XL-Eh1374), and Human PF T ELISA KIT (XL-Eh0770) were purchased from Abcam (Cambridge, MA, USA). ..

    Article Title: Decreased Gluconeogenesis in the Absence of Cystathionine Gamma-Lyase and the Underlying Mechanisms
    Article Snippet: .. Plasma glucagon was measured via the Glucagon (human/mouse/rat) EIA kit (Biovision) and corticosterone by Corticosterone ELISA (Abcam, Toronto, ON, Canada). ..

    Article Title: Social interaction reward in rats has anti‐stress effects, et al. Social interaction reward in rats has anti‐stress effects
    Article Snippet: .. 2.4 Urine corticosterone measurementsTwenty‐four hours after the CPP test, urine from rats was collected and quantitative corticosterone/creatinine measurements were performed using the corticosterone ELISA Kit (Abcam) according to the protocol provided with the kit. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Social support rescues acute stress-induced cognitive impairments by modulating ERK1/2 phosphorylation in adolescent mice
    Article Snippet: .. An Enzyme-Linked Immunosorbent Assay (ELISA) was performed to measure the level of corticosterone following the Corticosterone ELISA kit (CAT #. ab108821) from Abcam (Cambridge, United Kingdom). ..

    Concentration Assay:

    Article Title: Artificial Light at Night of Different Spectral Compositions Differentially Affects Tumor Growth in Mice: Interaction With Melatonin and Epigenetic Pathways
    Article Snippet: Concentrations (ng/mL) of 6-SMT were spectrophotometrically determined by ELISA microplate reader at 450 nm with reference wavelength of 650 nm (PowerWave HT; Biotek, Winooski, Vermont) and analyzed by Gen5 Data Analysis Software (version 2; Biotek). .. Urinary corticosterone concentration was determined using an ELISA kit (ab108821; Abcam, Cambridge, United Kingdom) with 96% recovery for corticosterone, 0.3 ng/mL sensitivity, and 5.0% CV intra-assay variation. ..

    Intra Assay:

    Article Title: Artificial Light at Night of Different Spectral Compositions Differentially Affects Tumor Growth in Mice: Interaction With Melatonin and Epigenetic Pathways
    Article Snippet: Concentrations (ng/mL) of 6-SMT were spectrophotometrically determined by ELISA microplate reader at 450 nm with reference wavelength of 650 nm (PowerWave HT; Biotek, Winooski, Vermont) and analyzed by Gen5 Data Analysis Software (version 2; Biotek). .. Urinary corticosterone concentration was determined using an ELISA kit (ab108821; Abcam, Cambridge, United Kingdom) with 96% recovery for corticosterone, 0.3 ng/mL sensitivity, and 5.0% CV intra-assay variation. ..

    Article Title: Neonatal repetitive pain in rats leads to impaired spatial learning and dysregulated hypothalamic-pituitary-adrenal axis function in later life
    Article Snippet: .. Serum corticosterone was measured using an enzyme-linked immunosorbent assay (ELISA) kit (ab108821, Abcam, Cambridge, UK), for which the sensitivity limit was 0.3 ng/ml, the intra-assay coefficient of variation was 5.0%, and the inter-assay coefficient was 7.2%. .. Serum ACTH was analyzed by electrochemiluminescence immunoassay (ECLIA) using a Roche Cobas® 6000 Analyzer (Roche Diagnostics GmbH, Mannheim, Germany).

    Inter Assay:

    Article Title: Neonatal repetitive pain in rats leads to impaired spatial learning and dysregulated hypothalamic-pituitary-adrenal axis function in later life
    Article Snippet: .. Serum corticosterone was measured using an enzyme-linked immunosorbent assay (ELISA) kit (ab108821, Abcam, Cambridge, UK), for which the sensitivity limit was 0.3 ng/ml, the intra-assay coefficient of variation was 5.0%, and the inter-assay coefficient was 7.2%. .. Serum ACTH was analyzed by electrochemiluminescence immunoassay (ECLIA) using a Roche Cobas® 6000 Analyzer (Roche Diagnostics GmbH, Mannheim, Germany).

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    Abcam elisa kit
    Detection of secreted albumin and blood urea nitrogen by <t>ELISA.</t> The <t>ALB</t> and BUN concentration of UC-MSCs and BM-MSCs culture supernatants collected on weeks 1, 2, 3, 4 and 5 following hepatic differentiation were measured. The medium of hepatocyte was measured as a control. Each bar represents the mean ± standard deviation (n=3). *P
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    93
    Abcam igfbp4
    Human fibrotic tissue shows altered patterns of BMP4 and <t>IGFBP4.</t> Human control peritoneum or thickened peritoneum samples from peritoneal dialysis patients were immunostained for HBME1 to identify mesothelium. Control peritoneum showed prominent mesothelial IGFBP4 and BMP4 immunostaining, which was attenuated in peritoneal dialysis samples. In the peritoneal dialysis samples, scattered IGFBP4‐positive cells were noted below the peritoneal surface. Scale bar: 100 μm.
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    Abcam elisa test kits
    Influence of quercetin on <t>STAT6</t> activation in HNEpCs after IL-4 stimulation in vitro . HNEpCs at 1 x 10 5 cells/ml are cultured with 10.0 ng/ml of IL-4 for 12 hours in the presence and absence of various concentrations of quercetin. STAT6 activation is measured using <t>ELISA,</t> and the results are expressed as the mean optical density at 450 nm ± SE of the triplicate cultures. The experiments are performed twice with similar results. Med. alone: medium alone; IL-4: interleukin-4; HNEpCs: human nasal epithelial cells.
    Elisa Test Kits, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Detection of secreted albumin and blood urea nitrogen by ELISA. The ALB and BUN concentration of UC-MSCs and BM-MSCs culture supernatants collected on weeks 1, 2, 3, 4 and 5 following hepatic differentiation were measured. The medium of hepatocyte was measured as a control. Each bar represents the mean ± standard deviation (n=3). *P

    Journal: Molecular Medicine Reports

    Article Title: Differentiation of umbilical cord mesenchymal stem cells into hepatocytes in comparison with bone marrow mesenchymal stem cells

    doi: 10.3892/mmr.2018.9181

    Figure Lengend Snippet: Detection of secreted albumin and blood urea nitrogen by ELISA. The ALB and BUN concentration of UC-MSCs and BM-MSCs culture supernatants collected on weeks 1, 2, 3, 4 and 5 following hepatic differentiation were measured. The medium of hepatocyte was measured as a control. Each bar represents the mean ± standard deviation (n=3). *P

    Article Snippet: ALB and blood urea nitrogen (BUN) contents were measured using ELISA kit (Human Albumin ELISA kit ab108788 and Bmassay, Human Blood Ureas Nitrogen ELISA kit 27013; Abcam, Cambridge, UK) according to the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

    Effect of Hericium erinaceus mycelium crude extract (HE-CE) and erinacine-S (E-S) on spinal nerve ligation (SNL)-induced elevation of plasma IL-6 levels of mice. Plasma was collected on day 13 ( n = 5–10). ∗∗∗ p

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Effects of Hericium erinaceus Mycelium Extracts on the Functional Activity of Purinoceptors and Neuropathic Pain in Mice with L5 Spinal Nerve Ligation

    doi: 10.1155/2020/2890194

    Figure Lengend Snippet: Effect of Hericium erinaceus mycelium crude extract (HE-CE) and erinacine-S (E-S) on spinal nerve ligation (SNL)-induced elevation of plasma IL-6 levels of mice. Plasma was collected on day 13 ( n = 5–10). ∗∗∗ p

    Article Snippet: Cytokine levels were determined using a mouse IL-6 ELISA Kit (Abcam, ab100712).

    Techniques: Ligation, Mouse Assay

    Human fibrotic tissue shows altered patterns of BMP4 and IGFBP4. Human control peritoneum or thickened peritoneum samples from peritoneal dialysis patients were immunostained for HBME1 to identify mesothelium. Control peritoneum showed prominent mesothelial IGFBP4 and BMP4 immunostaining, which was attenuated in peritoneal dialysis samples. In the peritoneal dialysis samples, scattered IGFBP4‐positive cells were noted below the peritoneal surface. Scale bar: 100 μm.

    Journal: The Journal of Pathology

    Article Title: Functional molecules in mesothelial‐to‐mesenchymal transition revealed by transcriptome analyses

    doi: 10.1002/path.5101

    Figure Lengend Snippet: Human fibrotic tissue shows altered patterns of BMP4 and IGFBP4. Human control peritoneum or thickened peritoneum samples from peritoneal dialysis patients were immunostained for HBME1 to identify mesothelium. Control peritoneum showed prominent mesothelial IGFBP4 and BMP4 immunostaining, which was attenuated in peritoneal dialysis samples. In the peritoneal dialysis samples, scattered IGFBP4‐positive cells were noted below the peritoneal surface. Scale bar: 100 μm.

    Article Snippet: In contrast, neither exogenous BMP4 nor IGFBP4 prevented the loss of cell–cell cingulin localization following addition of TGF‐β1 (Figure A).

    Techniques: Immunostaining

    Application of BMP4 or IGFBP4 to TGF‐β1‐exposed rat MCs. (A) Cells were maintained for 48 h in either basal medium alone (control), medium supplemented with 1 ng/ml TGF‐β1, or the latter supplemented with either 50 ng/ml BMP4 (TGF‐β1 + BMP4) or 50 ng/ml IGFBP4 (TGF‐β1 + IGFBP4). Cells were imaged by phase contrast microscopy (top row) or, as shown in subsequent rows, by immunofluorescence for ZO1, cingulin, and αSMA, with all nuclei counterstained with 4′,6‐diamidino‐2‐phenylindole (blue). Note that exposure to either BMP4 or IGFBP4 partially preserved the cobblestone pattern of the monolayer, reduced the cytoplasmic localization of ZO1, and reduced the level of αSMA. In contrast, neither factor rescued the TGF‐β1‐induced disruption of cingulin. Scale bars: 50 μm. (B) Quantification of αSMA by immunofluorescence, showing increased immunostaining in TGF‐β1‐treated versus control cells ( n = 5; mean ±SEM). There was a reduction in αSMA expression in cells treated with TGF‐β1 + BMP4 or TGF‐β1 + IGFBP4. (C) Phase contrast images showing MC migration into a wound over a period of 16 h under different conditions. Scale bars: 200 μm. (D) Quantification of MC migration under different conditions ( n = 6; mean ±SEM). Note that TGF‐β1 exposure was associated with more extensive migration than in controls ( n = 6), and that this effect was abrogated when either BMP4 or IGFBP4 was added with TGF‐β1.

    Journal: The Journal of Pathology

    Article Title: Functional molecules in mesothelial‐to‐mesenchymal transition revealed by transcriptome analyses

    doi: 10.1002/path.5101

    Figure Lengend Snippet: Application of BMP4 or IGFBP4 to TGF‐β1‐exposed rat MCs. (A) Cells were maintained for 48 h in either basal medium alone (control), medium supplemented with 1 ng/ml TGF‐β1, or the latter supplemented with either 50 ng/ml BMP4 (TGF‐β1 + BMP4) or 50 ng/ml IGFBP4 (TGF‐β1 + IGFBP4). Cells were imaged by phase contrast microscopy (top row) or, as shown in subsequent rows, by immunofluorescence for ZO1, cingulin, and αSMA, with all nuclei counterstained with 4′,6‐diamidino‐2‐phenylindole (blue). Note that exposure to either BMP4 or IGFBP4 partially preserved the cobblestone pattern of the monolayer, reduced the cytoplasmic localization of ZO1, and reduced the level of αSMA. In contrast, neither factor rescued the TGF‐β1‐induced disruption of cingulin. Scale bars: 50 μm. (B) Quantification of αSMA by immunofluorescence, showing increased immunostaining in TGF‐β1‐treated versus control cells ( n = 5; mean ±SEM). There was a reduction in αSMA expression in cells treated with TGF‐β1 + BMP4 or TGF‐β1 + IGFBP4. (C) Phase contrast images showing MC migration into a wound over a period of 16 h under different conditions. Scale bars: 200 μm. (D) Quantification of MC migration under different conditions ( n = 6; mean ±SEM). Note that TGF‐β1 exposure was associated with more extensive migration than in controls ( n = 6), and that this effect was abrogated when either BMP4 or IGFBP4 was added with TGF‐β1.

    Article Snippet: In contrast, neither exogenous BMP4 nor IGFBP4 prevented the loss of cell–cell cingulin localization following addition of TGF‐β1 (Figure A).

    Techniques: Microscopy, Immunofluorescence, Immunostaining, Expressing, Migration

    MMT is associated with dysregulation of the BMP4 and IGF pathways. (A) Volcano plot annotated with transcripts encoding molecules implicated in BMP and IGF signalling. (B) Confirmation of decreased levels of Bmp4 and Igfbp4 as assessed by qPCR ( n = 3; mean ±SEM). (C) As determined by ELISA, the levels of BMP4 and IGFBP4 were decreased in medium conditioned by cells exposed to 1 ng/ml TGF‐β1 ( n = 5; mean ±SEM).

    Journal: The Journal of Pathology

    Article Title: Functional molecules in mesothelial‐to‐mesenchymal transition revealed by transcriptome analyses

    doi: 10.1002/path.5101

    Figure Lengend Snippet: MMT is associated with dysregulation of the BMP4 and IGF pathways. (A) Volcano plot annotated with transcripts encoding molecules implicated in BMP and IGF signalling. (B) Confirmation of decreased levels of Bmp4 and Igfbp4 as assessed by qPCR ( n = 3; mean ±SEM). (C) As determined by ELISA, the levels of BMP4 and IGFBP4 were decreased in medium conditioned by cells exposed to 1 ng/ml TGF‐β1 ( n = 5; mean ±SEM).

    Article Snippet: In contrast, neither exogenous BMP4 nor IGFBP4 prevented the loss of cell–cell cingulin localization following addition of TGF‐β1 (Figure A).

    Techniques: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Influence of quercetin on STAT6 activation in HNEpCs after IL-4 stimulation in vitro . HNEpCs at 1 x 10 5 cells/ml are cultured with 10.0 ng/ml of IL-4 for 12 hours in the presence and absence of various concentrations of quercetin. STAT6 activation is measured using ELISA, and the results are expressed as the mean optical density at 450 nm ± SE of the triplicate cultures. The experiments are performed twice with similar results. Med. alone: medium alone; IL-4: interleukin-4; HNEpCs: human nasal epithelial cells.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Suppressive Effect of Quercetin on Nitric Oxide Production from Nasal Epithelial Cells In Vitro

    doi: 10.1155/2018/6097625

    Figure Lengend Snippet: Influence of quercetin on STAT6 activation in HNEpCs after IL-4 stimulation in vitro . HNEpCs at 1 x 10 5 cells/ml are cultured with 10.0 ng/ml of IL-4 for 12 hours in the presence and absence of various concentrations of quercetin. STAT6 activation is measured using ELISA, and the results are expressed as the mean optical density at 450 nm ± SE of the triplicate cultures. The experiments are performed twice with similar results. Med. alone: medium alone; IL-4: interleukin-4; HNEpCs: human nasal epithelial cells.

    Article Snippet: Assay for Transcription Factor Activation STAT6 activity in the cultured cells was examined by measuring the phosphorylated STAT6 level using commercially available ELISA test kits (Abcam plc., Cambridge, MA, USA), according to the manufacturer's recommended procedures.

    Techniques: Activation Assay, In Vitro, Cell Culture, Enzyme-linked Immunosorbent Assay