Journal: Frontiers in Pharmacology
Article Title: Therapeutic Effects of Tryptanthrin and Tryptanthrin-6-Oxime in Models of Rheumatoid Arthritis
Figure Lengend Snippet: Effect of TRYP and TRYP-Ox on cytokine production by LN cells. Starting at day 8 after CII challenge, mice were treated i.p. daily with saline (0), 30 mg/kg TRYP, or 30 mg/kg TRYP-Ox until day 42. Mice were euthanized, and their LN mononuclear cells were purified and cultured for 3 days with CII. Cytokine levels were measured in supernatants by ELISA. The results show the mean of one experiment (three to four replicates) ± S.D., which is representative of three independent experiments. a p
Article Snippet: Samples of the diluted sera were added to microtiter plates (Greiner Bio-One, Monroe, NC, USA) coated with 2 μg/ml ELISA grade mouse CII (Chondrex).
Techniques: Mouse Assay, Purification, Cell Culture, Enzyme-linked Immunosorbent Assay