elisa grade  (Chondrex inc)

Journal: Frontiers in Pharmacology

Article Title: Therapeutic Effects of Tryptanthrin and Tryptanthrin-6-Oxime in Models of Rheumatoid Arthritis

doi: 10.3389/fphar.2020.01145

Figure Lengend Snippet: Effect of TRYP and TRYP-Ox on cytokine production by LN cells. Starting at day 8 after CII challenge, mice were treated i.p. daily with saline (0), 30 mg/kg TRYP, or 30 mg/kg TRYP-Ox until day 42. Mice were euthanized, and their LN mononuclear cells were purified and cultured for 3 days with CII. Cytokine levels were measured in supernatants by ELISA. The results show the mean of one experiment (three to four replicates) ± S.D., which is representative of three independent experiments. a p

Article Snippet: Samples of the diluted sera were added to microtiter plates (Greiner Bio-One, Monroe, NC, USA) coated with 2 μg/ml ELISA grade mouse CII (Chondrex).

Techniques: Mouse Assay, Purification, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Arthritis & rheumatology (Hoboken, N.J.)

Article Title: Modulation of inflammatory arthritis by gut microbiota through mucosal inflammation and autoantibody generation

doi: 10.1002/art.40490

Figure Lengend Snippet: Microbiota alter the ability of serum autoantibodies to activate complement Activation of the classical complement pathway by day 35 serum anti-CII antibodies from mice with CIA or mice with CIA treated with antibiotics was assessed by a modified ELISA described in Methods. C3 activation is shown as the mean U/ml ± SEM. N=6–11 per group. Statistical significance was determined by a two-tailed Student’s t-test. *, p

Article Snippet: 96-well plates (Costar, Corning) were coated overnight at 4°C with 5 μg/mL ELISA grade mouse CII (Chondrex® ).

Techniques: Activation Assay, Mouse Assay, Modification, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Deficiency of STING Promotes Collagen-Specific Antibody Production and B Cell Survival in Collagen-Induced Arthritis

doi: 10.3389/fimmu.2020.01101

Figure Lengend Snippet: The STING-deficient mice developed a higher production of anti-collagen type 2 antibody. The level of anti-collagen type 2 antibodies detecting by the ELISA technique after the 1st immunization for 3 weeks, and the 2nd immunization for 3 weeks are shown on (A–D) . The isotypes of anti-collagen type 2 antibodies in (A) IgM, (B) IgG, (C) IgG 2b , and (D) IgG 2c are shown in concentration as mean ± SEM; N = 5 mice/group in CFA control and N = −11 mice/group in CFA+CII treatment (* p

Article Snippet: Detection of Anti-collagen Type 2 Specific Antibody Chick collagen type 2 ELISA grade (#2011; Chondrex Redmond, WA) was coated on the plate overnight at 4°C.

Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Frontiers in Immunology

Article Title: Deficiency of STING Promotes Collagen-Specific Antibody Production and B Cell Survival in Collagen-Induced Arthritis

doi: 10.3389/fimmu.2020.01101

Figure Lengend Snippet: STING was not necessary for arthritis development in the CIA model. The mice were injected with CFA or CFA plus chicken collagen type 2 on days 0 and 21. The clinical sign of arthritis and pathology were observed. (A) The representative of hind paws from WT and Sting gt / gt mice, and (B) the cumulative incidence of arthritis from WT and Sting gt / gt mice are shown. (C,D) Arthritis scores were blindly graded every other day. Data are shown as the median on (C) line chart and (D) interquartile range (* p

Article Snippet: Detection of Anti-collagen Type 2 Specific Antibody Chick collagen type 2 ELISA grade (#2011; Chondrex Redmond, WA) was coated on the plate overnight at 4°C.

Techniques: Mouse Assay, Injection