elisa assay tgf β levels  (Boster Bio)


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    Name:
    Human TGFBR2 Tgf Beta Receptor Ii TGF beta R2 ELISA Kit PicoKine
    Description:

    Catalog Number:
    EK1307
    Price:
    369.0
    Category:
    ELISA Kits
    Reactivity:
    Human
    Applications:
    ELISA
    Immunogen:
    Expression system for standard: NSO; Immunogen sequence: I24-D159
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    Structured Review

    Boster Bio elisa assay tgf β levels
    Human TGFBR2 Tgf Beta Receptor Ii TGF beta R2 ELISA Kit PicoKine

    https://www.bioz.com/result/elisa assay tgf β levels/product/Boster Bio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa assay tgf β levels - by Bioz Stars, 2021-07
    91/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Human Umbilical Cord MSC-Derived Exosomes Suppress the Development of CCl4-Induced Liver Injury through Antioxidant Effect
    Article Snippet: MDA levels were normalized to milligrams of protein. .. ELISA Assay TGF-β levels in fibrotic liver tissues treated with PBS or hucMSC-Ex were determined using an ELISA kit (Boster, Wuhan, China) according to the manufacturer's instructions. .. TUNEL Assay The apoptotic cells in liver slides were measured by using an in situ cell apoptosis kit (Vazyme, Nanjing, China) according to the manufacturer's instructions.

    Article Title: Ganoderma lucidum polysaccharides reduce methotrexate-induced small intestinal damage in mice via induction of epithelial cell proliferation and migration
    Article Snippet: MTX was purchased from Zhejiang Hengrui Pharmaceutical Co, Ltd. Malondialdehyde (MDA) and superoxide dismutase (SOD) analysis kits were purchased from the Nanjing Jiancheng Biology Research Center. .. The TGFβ ELISA kit was purchased from Wuhan Boster Bio-engineering Co, Ltd. Primary, and secondary antibodies for the measurement of IgA were obtained from Sigma Chemical Co. ..

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    Boster Bio tgf β1
    BCG decreased the levels of <t>TGF-β1</t> in BALF and sera in a rat model of asthma as analyzed by ELISA. Values are expressed as mean ± SD (n = 8) Significant at: # P
    Tgf β1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tgf β1/product/Boster Bio
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tgf β1 - by Bioz Stars, 2021-07
    96/100 stars
      Buy from Supplier

    94
    Boster Bio tgf β levels
    Effects of alcohol and high-fat diet on the total amount of blood vessels (a) and hemosiderin deposits (b) and <t>TGF-</t> <t>β</t> levels (c) in skin wounds from Wistar rats. C1 = control 1—water via gavage and standard chow diet; C2 = control 2—water (no gavage) and standard chow diet; AL = alcohol—water (no gavage), alcohol (40%) via gavage and standard chow diet; HF = high-fat—water (no gavage) and high-fat diet (50%); HF + AL = alcohol/high-fat—water (no gavage), alcohol (40%) via gavage and high-fat diet. F0 = intact tissue; F1, F2, and F3 = scar tissue after 7, 14, and 21 days, respectively. ∗ indicates statistical differences versus C1 and C2; # indicates statistical difference versus C1, C2, AL, and HF; indicates statistical differences versus AL and HF + AL.
    Tgf β Levels, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tgf β levels/product/Boster Bio
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tgf β levels - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

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    BCG decreased the levels of TGF-β1 in BALF and sera in a rat model of asthma as analyzed by ELISA. Values are expressed as mean ± SD (n = 8) Significant at: # P

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Bacillus Calmette-Guerin alleviates airway inflammation and remodeling by preventing TGF-β1 induced epithelial–mesenchymal transition

    doi: 10.1080/21645515.2017.1313366

    Figure Lengend Snippet: BCG decreased the levels of TGF-β1 in BALF and sera in a rat model of asthma as analyzed by ELISA. Values are expressed as mean ± SD (n = 8) Significant at: # P

    Article Snippet: Levels of TGF-β1 in sera and BALF were measured by ELISA-kit (Boster Biological Company, Wuhan, China), following the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay

    BCG inhibits TGF-β1- induced EMT in a rat model of asthma. (A-C) Immunohistochemistry images of E-cadherin(A), α-SMA(B), and Fibronectin(C) staining in pulmonary tissue slices of all 7 groups. (D) Quantitative analysis of the western blot results of E-cadherin, α-SMA, and Fibronectin by relative densitometry intensity. Values are expressed as mean ± SD (n = 8) Significant at: # P

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: Bacillus Calmette-Guerin alleviates airway inflammation and remodeling by preventing TGF-β1 induced epithelial–mesenchymal transition

    doi: 10.1080/21645515.2017.1313366

    Figure Lengend Snippet: BCG inhibits TGF-β1- induced EMT in a rat model of asthma. (A-C) Immunohistochemistry images of E-cadherin(A), α-SMA(B), and Fibronectin(C) staining in pulmonary tissue slices of all 7 groups. (D) Quantitative analysis of the western blot results of E-cadherin, α-SMA, and Fibronectin by relative densitometry intensity. Values are expressed as mean ± SD (n = 8) Significant at: # P

    Article Snippet: Levels of TGF-β1 in sera and BALF were measured by ELISA-kit (Boster Biological Company, Wuhan, China), following the manufacturer's instructions.

    Techniques: Immunohistochemistry, Staining, Western Blot

    Irradiation induced TGF-β1 production via NF-κB activation, and MSCCM prevented NF-κB activation in HCF cells. (A) HCF cells were pre-incubated with NF-κB inhibitor MG-132 (5 μM) for 60 min before irradiation, and the level of TGF-β1 in cell lysate of HCF cells was determined using commercially available ELISA kits in control cells (Control), single-irradiated HCF cells (Ir), and irradiation + NF-κB inhibitor–treated HCF cells (Ir+ NF-κB inhibitor). (B) HCF cells were collected, and p65 amounts in nuclear protein extracts were analyzed by ELISA kit in control cells (Control), single-irradiated HCF cells (Ir), and irradiation + MSCCM–treated HCF cells (Ir+MSCCM). Data are expressed as the mean ± SD ( n = 5). ** P

    Journal: Journal of Radiation Research

    Article Title: The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells

    doi: 10.1093/jrr/rry048

    Figure Lengend Snippet: Irradiation induced TGF-β1 production via NF-κB activation, and MSCCM prevented NF-κB activation in HCF cells. (A) HCF cells were pre-incubated with NF-κB inhibitor MG-132 (5 μM) for 60 min before irradiation, and the level of TGF-β1 in cell lysate of HCF cells was determined using commercially available ELISA kits in control cells (Control), single-irradiated HCF cells (Ir), and irradiation + NF-κB inhibitor–treated HCF cells (Ir+ NF-κB inhibitor). (B) HCF cells were collected, and p65 amounts in nuclear protein extracts were analyzed by ELISA kit in control cells (Control), single-irradiated HCF cells (Ir), and irradiation + MSCCM–treated HCF cells (Ir+MSCCM). Data are expressed as the mean ± SD ( n = 5). ** P

    Article Snippet: The levels of transforming growth factor-β1 (TGF-β1), interleukin-6 (IL-6), interleukin-8 (IL-8) and interferon-γ (IFN-γ) in cell lysate of HCF cells were determined using commercially available ELISA kits according to the manufacturer’s protocols (Boster Biological Technology, Wuhan, China).

    Techniques: Irradiation, Activation Assay, Incubation, Enzyme-linked Immunosorbent Assay

    MSCCM improved the inflammation status in HCF cells. HCF cells were collected, and the levels of TGF-β1 (A), IL-6 (C), IL-8 (E) and IFN-γ (G) in cell lysate of HCF cells were determined using commercially available ELISA kits in control cells (Control), single-irradiated HCF cells (Ir), and irradiation + MSCCM–treated HCF cells (Ir+MSCCM). The mRNA levels of TGF-β1, IL-6, IL-8 and IFN-γ were examined by q-PCR. The expression of each mRNA was calculated as 2 −ΔΔct and the mRNA levels of TGF-β1 (B), IL-6 (D), IL-8 (F) and IFN-γ (H) were normalized with the mRNA levels of GAPDH. Data are expressed as the mean ± SD ( n = 5). ** P

    Journal: Journal of Radiation Research

    Article Title: The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells

    doi: 10.1093/jrr/rry048

    Figure Lengend Snippet: MSCCM improved the inflammation status in HCF cells. HCF cells were collected, and the levels of TGF-β1 (A), IL-6 (C), IL-8 (E) and IFN-γ (G) in cell lysate of HCF cells were determined using commercially available ELISA kits in control cells (Control), single-irradiated HCF cells (Ir), and irradiation + MSCCM–treated HCF cells (Ir+MSCCM). The mRNA levels of TGF-β1, IL-6, IL-8 and IFN-γ were examined by q-PCR. The expression of each mRNA was calculated as 2 −ΔΔct and the mRNA levels of TGF-β1 (B), IL-6 (D), IL-8 (F) and IFN-γ (H) were normalized with the mRNA levels of GAPDH. Data are expressed as the mean ± SD ( n = 5). ** P

    Article Snippet: The levels of transforming growth factor-β1 (TGF-β1), interleukin-6 (IL-6), interleukin-8 (IL-8) and interferon-γ (IFN-γ) in cell lysate of HCF cells were determined using commercially available ELISA kits according to the manufacturer’s protocols (Boster Biological Technology, Wuhan, China).

    Techniques: Enzyme-linked Immunosorbent Assay, Irradiation, Polymerase Chain Reaction, Expressing

    Renal expression of AT1 (a) and TGF- β 1 (b) in ZL and ZDF rats, and levels of AT1 (c) and TGF- β 1 (d) mRNAs, and TGF- β protein (e) in rat mesangial cells. In cell culture, SO (SO10: 10; SO20: 20 and SO40: 40 μ g/ml), MA (25 μ M) or telmisartan (Tel. 10 μ M) was added 1 h prior to treatment with angiotensin II (10 −6 M), followed by twenty-four hours incubation. Total RNA was extracted from renal tissues and the mesangial cells using TRIzol, respectively. The relative levels of specific mRNAs were determined by RT–PCR. Results were normalized to β -actin. In a parallel experiment, the medium was collected after mesangial cells were treated with the test agents in combination with angiotensin II. TGF- β protein level was determined by commercial ELISA kit according to the manufacturer's instructions. All values are means ± SEM ( n = 5, each group in vivo ; n = 3, each group in vitro ). * P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Ayurvedic Medicine Salacia oblonga Attenuates Diabetic Renal Fibrosis in Rats: Suppression of Angiotensin II/AT1 Signaling

    doi: 10.1093/ecam/nep095

    Figure Lengend Snippet: Renal expression of AT1 (a) and TGF- β 1 (b) in ZL and ZDF rats, and levels of AT1 (c) and TGF- β 1 (d) mRNAs, and TGF- β protein (e) in rat mesangial cells. In cell culture, SO (SO10: 10; SO20: 20 and SO40: 40 μ g/ml), MA (25 μ M) or telmisartan (Tel. 10 μ M) was added 1 h prior to treatment with angiotensin II (10 −6 M), followed by twenty-four hours incubation. Total RNA was extracted from renal tissues and the mesangial cells using TRIzol, respectively. The relative levels of specific mRNAs were determined by RT–PCR. Results were normalized to β -actin. In a parallel experiment, the medium was collected after mesangial cells were treated with the test agents in combination with angiotensin II. TGF- β protein level was determined by commercial ELISA kit according to the manufacturer's instructions. All values are means ± SEM ( n = 5, each group in vivo ; n = 3, each group in vitro ). * P

    Article Snippet: Twenty-four hours later, the medium was collected for determination of fibronectin and transforming growth factor (TGF-β ) protein levels by ELISA (Boster) according to the manufacturer's instructions.

    Techniques: Expressing, Cell Culture, Incubation, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, In Vivo, In Vitro

    Effects of alcohol and high-fat diet on the total amount of blood vessels (a) and hemosiderin deposits (b) and TGF- β levels (c) in skin wounds from Wistar rats. C1 = control 1—water via gavage and standard chow diet; C2 = control 2—water (no gavage) and standard chow diet; AL = alcohol—water (no gavage), alcohol (40%) via gavage and standard chow diet; HF = high-fat—water (no gavage) and high-fat diet (50%); HF + AL = alcohol/high-fat—water (no gavage), alcohol (40%) via gavage and high-fat diet. F0 = intact tissue; F1, F2, and F3 = scar tissue after 7, 14, and 21 days, respectively. ∗ indicates statistical differences versus C1 and C2; # indicates statistical difference versus C1, C2, AL, and HF; indicates statistical differences versus AL and HF + AL.

    Journal: Mediators of Inflammation

    Article Title: High-Fat Diet and Alcohol Intake Promotes Inflammation and Impairs Skin Wound Healing in Wistar Rats

    doi: 10.1155/2018/4658583

    Figure Lengend Snippet: Effects of alcohol and high-fat diet on the total amount of blood vessels (a) and hemosiderin deposits (b) and TGF- β levels (c) in skin wounds from Wistar rats. C1 = control 1—water via gavage and standard chow diet; C2 = control 2—water (no gavage) and standard chow diet; AL = alcohol—water (no gavage), alcohol (40%) via gavage and standard chow diet; HF = high-fat—water (no gavage) and high-fat diet (50%); HF + AL = alcohol/high-fat—water (no gavage), alcohol (40%) via gavage and high-fat diet. F0 = intact tissue; F1, F2, and F3 = scar tissue after 7, 14, and 21 days, respectively. ∗ indicates statistical differences versus C1 and C2; # indicates statistical difference versus C1, C2, AL, and HF; indicates statistical differences versus AL and HF + AL.

    Article Snippet: Expression of Inflammatory Cytokine TGF-β Scar tissue samples collected on days 7 and 14 were frozen at −80°C and homogenized in PBS buffer (pH 7.4) containing Tween (0.05%) and centrifuged at 3500g for 30 min. TGF-β levels in the supernatant were analyzed using ELISA immunoassay kits (Boster Biological Technology Ltd., China), following the manufacturer's protocol.

    Techniques: