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Shimadzu Corporation electrospray ionization tandem mass spectrometry
Electrospray Ionization Tandem Mass Spectrometry, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 8 article reviews
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electrospray ionization tandem mass spectrometry - by Bioz Stars, 2020-09
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High Performance Liquid Chromatography:

Article Title: Association between inflammation and skeletal muscle proteolysis, skeletal mass and strength in elderly heart failure patients and their prognostic implications
Article Snippet: .. The 3-MH in the urine was measured by a precolumn derivatization using reagent 3-aminopyridyl-N -hydroxysuccinimidyl carbamate (APDSTAG WAKO, FUJIFILM WAKO Inc. Osaka, Japan), followed by reversed-phase high-performance liquid chromatography and electrospray ionization tandem mass spectrometry (LCMS-2020, Shimadzu Corporation, Kyoto, Japan). ..

Article Title: Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose–xylose co-substrate
Article Snippet: .. The metabolites were quantified by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry combined with the method package to measure primary metabolites (LC–MS-8040 triple quadrupole LC/MS/MS spectrometer; Shimadzu, Kyoto, Japan). .. Quantification of transcriptional level The transcriptional expression of maeB, ppsA , ppc , pckA , pgi , zwf , gltA , and arcA in each strain was quantified using real-time PCR.

Article Title: Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration
Article Snippet: .. Sphingolipids were quantified by liquid chromatography, electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a Shimadzu LC-20AD binary pump HPLC system (Shimadzu, Kyoto, Japan) and an Applied Biosystems 4000 QTRAP operating in a triple quadrupole mode, as described previously ( ). .. In some experiments, cell extracts were immunoprecipitated with anti-PHB1 or anti-PHB2 antibody or with control IgG, as described above, and bound sphingolipids were determined by LC-ESI-MS/MS.

Article Title: Trimethylamine N‐Oxide and Mortality Risk in Patients With Peripheral Artery Disease
Article Snippet: .. TMAO, choline, and betaine levels in plasma were determined using stable isotope dilution high‐performance liquid chromatography with online electrospray ionization tandem mass spectrometry on Shimadzu LCMS‐8050 CL Triple Quadrupole mass spectrometer (Shimadzu Corp, Kyoto, Japan) using d9‐(trimethyl)‐labeled internal standards as described previously., ..

Article Title: Anionic metabolite biosynthesis enhanced by potassium under dark, anaerobic conditions in cyanobacteria
Article Snippet: .. The metabolites were quantified by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LCMS-8040 triple quadrupole LC/MS/MS spectrometer; Shimadzu, Kyoto, Japan). .. Gas chromatography mass spectrometry (GC-MS) analysis Cells were incubated under dark, anaerobic conditions as wells as the experiment of organic acid production.

Liquid Chromatography with Mass Spectroscopy:

Article Title: Association between inflammation and skeletal muscle proteolysis, skeletal mass and strength in elderly heart failure patients and their prognostic implications
Article Snippet: .. The 3-MH in the urine was measured by a precolumn derivatization using reagent 3-aminopyridyl-N -hydroxysuccinimidyl carbamate (APDSTAG WAKO, FUJIFILM WAKO Inc. Osaka, Japan), followed by reversed-phase high-performance liquid chromatography and electrospray ionization tandem mass spectrometry (LCMS-2020, Shimadzu Corporation, Kyoto, Japan). ..

Article Title: Low-dose carbon-based nanoparticle-induced effects in A549 lung cells determined by biospectroscopy are associated with increases in genomic methylation
Article Snippet: .. For the mass spectrometric analysis, an electrospray ionization tandem mass spectrometry (LCMS-8030, Shimadzu, Japan) system was used, operating in positive ionization mode and conditioned at a capillary temperature of 400 °C and medium N2 curtain gas. ..

Article Title: Trimethylamine N‐Oxide and Mortality Risk in Patients With Peripheral Artery Disease
Article Snippet: .. TMAO, choline, and betaine levels in plasma were determined using stable isotope dilution high‐performance liquid chromatography with online electrospray ionization tandem mass spectrometry on Shimadzu LCMS‐8050 CL Triple Quadrupole mass spectrometer (Shimadzu Corp, Kyoto, Japan) using d9‐(trimethyl)‐labeled internal standards as described previously., ..

Article Title: Anionic metabolite biosynthesis enhanced by potassium under dark, anaerobic conditions in cyanobacteria
Article Snippet: .. The metabolites were quantified by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LCMS-8040 triple quadrupole LC/MS/MS spectrometer; Shimadzu, Kyoto, Japan). .. Gas chromatography mass spectrometry (GC-MS) analysis Cells were incubated under dark, anaerobic conditions as wells as the experiment of organic acid production.

Isolation:

Article Title: A Serotype 5/3 Adenovirus Expressing MDA-7/IL-24 Infects Renal Carcinoma Cells and Promotes Toxicity of Agents That Increase Ros and Ceramide Levels
Article Snippet: .. Lipids were isolated from the cells, and ceramide isoforms were analyzed by liquid chromatography and electrospray ionization-tandem mass spectrometry with a binary pump system (LC-10; Shimadzu, Columbia, MD) coupled to a PerkinElmer Series 200 autoinjector (PerkinElmer Life and Analytical Sciences, Waltham, MA) and a mass spectrometer (4000 Q TRAP; Applied Biosystems, Foster City, CA) operating in a triple quadrupole mode, as described previously ( ). .. Athymic Nu/Nu mice (8 weeks old, female) were obtained from the NCI.

Isotope Dilution:

Article Title: Trimethylamine N‐Oxide and Mortality Risk in Patients With Peripheral Artery Disease
Article Snippet: .. TMAO, choline, and betaine levels in plasma were determined using stable isotope dilution high‐performance liquid chromatography with online electrospray ionization tandem mass spectrometry on Shimadzu LCMS‐8050 CL Triple Quadrupole mass spectrometer (Shimadzu Corp, Kyoto, Japan) using d9‐(trimethyl)‐labeled internal standards as described previously., ..

Mass Spectrometry:

Article Title: Association between inflammation and skeletal muscle proteolysis, skeletal mass and strength in elderly heart failure patients and their prognostic implications
Article Snippet: .. The 3-MH in the urine was measured by a precolumn derivatization using reagent 3-aminopyridyl-N -hydroxysuccinimidyl carbamate (APDSTAG WAKO, FUJIFILM WAKO Inc. Osaka, Japan), followed by reversed-phase high-performance liquid chromatography and electrospray ionization tandem mass spectrometry (LCMS-2020, Shimadzu Corporation, Kyoto, Japan). ..

Article Title: Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose–xylose co-substrate
Article Snippet: .. The metabolites were quantified by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry combined with the method package to measure primary metabolites (LC–MS-8040 triple quadrupole LC/MS/MS spectrometer; Shimadzu, Kyoto, Japan). .. Quantification of transcriptional level The transcriptional expression of maeB, ppsA , ppc , pckA , pgi , zwf , gltA , and arcA in each strain was quantified using real-time PCR.

Article Title: A Serotype 5/3 Adenovirus Expressing MDA-7/IL-24 Infects Renal Carcinoma Cells and Promotes Toxicity of Agents That Increase Ros and Ceramide Levels
Article Snippet: .. Lipids were isolated from the cells, and ceramide isoforms were analyzed by liquid chromatography and electrospray ionization-tandem mass spectrometry with a binary pump system (LC-10; Shimadzu, Columbia, MD) coupled to a PerkinElmer Series 200 autoinjector (PerkinElmer Life and Analytical Sciences, Waltham, MA) and a mass spectrometer (4000 Q TRAP; Applied Biosystems, Foster City, CA) operating in a triple quadrupole mode, as described previously ( ). .. Athymic Nu/Nu mice (8 weeks old, female) were obtained from the NCI.

Article Title: Sphingosine-1-phosphate phosphohydrolase-1 regulates ER stress-induced autophagy
Article Snippet: .. Sphingolipids were quantified by liquid chromatography, electrospray ionization-tandem mass spectrometry with a Shimadzu (Columbia, MD, USA) LC-10 binary pump system coupled to a PerkinElmer Series 200 autoinjector and an Applied Biosystems 4000 QTRAP operating in a triple quadrupole mode, as described previously. ..

Article Title: Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration
Article Snippet: .. Sphingolipids were quantified by liquid chromatography, electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a Shimadzu LC-20AD binary pump HPLC system (Shimadzu, Kyoto, Japan) and an Applied Biosystems 4000 QTRAP operating in a triple quadrupole mode, as described previously ( ). .. In some experiments, cell extracts were immunoprecipitated with anti-PHB1 or anti-PHB2 antibody or with control IgG, as described above, and bound sphingolipids were determined by LC-ESI-MS/MS.

Article Title: Low-dose carbon-based nanoparticle-induced effects in A549 lung cells determined by biospectroscopy are associated with increases in genomic methylation
Article Snippet: .. For the mass spectrometric analysis, an electrospray ionization tandem mass spectrometry (LCMS-8030, Shimadzu, Japan) system was used, operating in positive ionization mode and conditioned at a capillary temperature of 400 °C and medium N2 curtain gas. ..

Article Title: Trimethylamine N‐Oxide and Mortality Risk in Patients With Peripheral Artery Disease
Article Snippet: .. TMAO, choline, and betaine levels in plasma were determined using stable isotope dilution high‐performance liquid chromatography with online electrospray ionization tandem mass spectrometry on Shimadzu LCMS‐8050 CL Triple Quadrupole mass spectrometer (Shimadzu Corp, Kyoto, Japan) using d9‐(trimethyl)‐labeled internal standards as described previously., ..

Article Title: Anionic metabolite biosynthesis enhanced by potassium under dark, anaerobic conditions in cyanobacteria
Article Snippet: .. The metabolites were quantified by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LCMS-8040 triple quadrupole LC/MS/MS spectrometer; Shimadzu, Kyoto, Japan). .. Gas chromatography mass spectrometry (GC-MS) analysis Cells were incubated under dark, anaerobic conditions as wells as the experiment of organic acid production.

Liquid Chromatography:

Article Title: A Serotype 5/3 Adenovirus Expressing MDA-7/IL-24 Infects Renal Carcinoma Cells and Promotes Toxicity of Agents That Increase Ros and Ceramide Levels
Article Snippet: .. Lipids were isolated from the cells, and ceramide isoforms were analyzed by liquid chromatography and electrospray ionization-tandem mass spectrometry with a binary pump system (LC-10; Shimadzu, Columbia, MD) coupled to a PerkinElmer Series 200 autoinjector (PerkinElmer Life and Analytical Sciences, Waltham, MA) and a mass spectrometer (4000 Q TRAP; Applied Biosystems, Foster City, CA) operating in a triple quadrupole mode, as described previously ( ). .. Athymic Nu/Nu mice (8 weeks old, female) were obtained from the NCI.

Article Title: Sphingosine-1-phosphate phosphohydrolase-1 regulates ER stress-induced autophagy
Article Snippet: .. Sphingolipids were quantified by liquid chromatography, electrospray ionization-tandem mass spectrometry with a Shimadzu (Columbia, MD, USA) LC-10 binary pump system coupled to a PerkinElmer Series 200 autoinjector and an Applied Biosystems 4000 QTRAP operating in a triple quadrupole mode, as described previously. ..

Article Title: Sphingosine-1-phosphate produced by sphingosine kinase 2 in mitochondria interacts with prohibitin 2 to regulate complex IV assembly and respiration
Article Snippet: .. Sphingolipids were quantified by liquid chromatography, electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) with a Shimadzu LC-20AD binary pump HPLC system (Shimadzu, Kyoto, Japan) and an Applied Biosystems 4000 QTRAP operating in a triple quadrupole mode, as described previously ( ). .. In some experiments, cell extracts were immunoprecipitated with anti-PHB1 or anti-PHB2 antibody or with control IgG, as described above, and bound sphingolipids were determined by LC-ESI-MS/MS.

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  • 90
    Shimadzu Corporation esi ms ms analysis
    Activity of recombinant bacterial UDP-apiose synthase (bUAS) by in microbe assay. Analysis of in microbe nucleotide sugars by <t>HILIC-LC-ESI-MS/MS.</t> (A) top panel elution of standard (Std): UDP-GlcA, UDP-Xyl and UDP-arabinopyranose (UDP-Ara p ); Nucleotide sugars were extracted from E . coli cells induced to express genes encoding CeUAS, GrUAS, XpUAS or empty vector as the control (bottom panel). [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed. Park’s nucleotide is a UDP-MurNAc-pentapeptide that is used as an internal standard for nucleotide-sugar detection as it is abundantly made in E . coli . The m/z signal for CeUAS and XpUAS is amplified by a factor of 10. (B) Second stage MS fragmentation data for the peaks at the indicated retention times; Left column 11.0 min and right column 12.0 min. MS/MS ions at m/z 323.0, 211.0, 403.0 are consistent with predicted fragmentation of a UDP-sugar into [UMP-H] - , [Ura-2H] - , and [UDP-H 2 O-H] - , respectively.
    Esi Ms Ms Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/esi ms ms analysis/product/Shimadzu Corporation
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    esi ms ms analysis - by Bioz Stars, 2020-09
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    90
    Shimadzu Corporation uhplc esi ms ms analyses
    Analysis of CGA and CSF samples in the rats treated with water extract of E. ulmoides (EUWE) by <t>UHPLC-ESI-MS/MS</t> chromatogram. ( A ) Positive Scan m / z 100–500 of CGA; ( B ) Negative Scan m / z 100–500 of CGA; ( C ) MS/MS spectrum of m / z 353.10 of CGA in the negative ion mode; ( D ) MRM of channels ESI − 353.10/191.15 for CGA and CSF samples in rat treated with EUWE; ( E ) MRM of channels ESI − 353.10/179.00 for CGA and CSF samples in the rat treated with EUWE. Notes: chromatographic conditions are provided in the Experimental Section. The rats were treated with EUWE at 4.0 g/kg.
    Uhplc Esi Ms Ms Analyses, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uhplc esi ms ms analyses/product/Shimadzu Corporation
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    uhplc esi ms ms analyses - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    88
    Shimadzu Corporation electrospray ionization esi liquid chromatography lc ms ms
    Effects of chronic running exercise on α-oxoaldehydes in Zucker diabetic fatty rats. (A) Urinary methylglyoxal (MG) in sedentary lean Zucker diabetic fatty (ZDF) rats (Sed-ZL), sedentary ZDF rats (Sed-ZDF) and aerobic treadmill exercised ZDF rats (Ex-ZDF) groups was assessed by <t>electrospray</t> ionization <t>(ESI)</t> liquid chromatography (LC)-mass spectrometry (MS) (LC-MS/MS), and expressed as urinary MG:creatinine ratio. (B) MG, (C) 3-deoxy-D-glucosone (3DG) and (D) glyoxal (GO) in the renal cortex of Sed-ZL, Sed-ZDF and Ex-ZDF groups were assessed by LC-MS/MS. Values are means ± SEM ( n = 5/group). * p
    Electrospray Ionization Esi Liquid Chromatography Lc Ms Ms, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrospray ionization esi liquid chromatography lc ms ms/product/Shimadzu Corporation
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    electrospray ionization esi liquid chromatography lc ms ms - by Bioz Stars, 2020-09
    88/100 stars
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    Shimadzu Corporation hplc esi ms ms analysis
    Total ion chromatorgraphies of CS aqueous extract by <t>HPLC-ESI-MS/MS</t> analysis. (a) Positive ion mode. (b) Negative ion mode.
    Hplc Esi Ms Ms Analysis, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc esi ms ms analysis/product/Shimadzu Corporation
    Average 90 stars, based on 3 article reviews
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    Activity of recombinant bacterial UDP-apiose synthase (bUAS) by in microbe assay. Analysis of in microbe nucleotide sugars by HILIC-LC-ESI-MS/MS. (A) top panel elution of standard (Std): UDP-GlcA, UDP-Xyl and UDP-arabinopyranose (UDP-Ara p ); Nucleotide sugars were extracted from E . coli cells induced to express genes encoding CeUAS, GrUAS, XpUAS or empty vector as the control (bottom panel). [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed. Park’s nucleotide is a UDP-MurNAc-pentapeptide that is used as an internal standard for nucleotide-sugar detection as it is abundantly made in E . coli . The m/z signal for CeUAS and XpUAS is amplified by a factor of 10. (B) Second stage MS fragmentation data for the peaks at the indicated retention times; Left column 11.0 min and right column 12.0 min. MS/MS ions at m/z 323.0, 211.0, 403.0 are consistent with predicted fragmentation of a UDP-sugar into [UMP-H] - , [Ura-2H] - , and [UDP-H 2 O-H] - , respectively.

    Journal: PLoS ONE

    Article Title: Synthesis of UDP-apiose in Bacteria: The marine phototroph Geminicoccus roseus and the plant pathogen Xanthomonas pisi

    doi: 10.1371/journal.pone.0184953

    Figure Lengend Snippet: Activity of recombinant bacterial UDP-apiose synthase (bUAS) by in microbe assay. Analysis of in microbe nucleotide sugars by HILIC-LC-ESI-MS/MS. (A) top panel elution of standard (Std): UDP-GlcA, UDP-Xyl and UDP-arabinopyranose (UDP-Ara p ); Nucleotide sugars were extracted from E . coli cells induced to express genes encoding CeUAS, GrUAS, XpUAS or empty vector as the control (bottom panel). [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed. Park’s nucleotide is a UDP-MurNAc-pentapeptide that is used as an internal standard for nucleotide-sugar detection as it is abundantly made in E . coli . The m/z signal for CeUAS and XpUAS is amplified by a factor of 10. (B) Second stage MS fragmentation data for the peaks at the indicated retention times; Left column 11.0 min and right column 12.0 min. MS/MS ions at m/z 323.0, 211.0, 403.0 are consistent with predicted fragmentation of a UDP-sugar into [UMP-H] - , [Ura-2H] - , and [UDP-H 2 O-H] - , respectively.

    Article Snippet: HILIC-LC-ESI-MS/MS ESI-MS/MS analysis was performed on a Shimadzu (Kyoto, Japan) LC-ESI-MS-IT-TOF operating in the negative ion mode.

    Techniques: Activity Assay, Recombinant, Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry, Acetylene Reduction Assay, Plasmid Preparation, Diagnostic Assay, Amplification

    Detection of bUAS transcripts and UDP-apiose. In vivo indication for the functional activity of bUAS genes and enzymes. (A) RT-PCR analyses showing bUAS transcripts of G . roseus and X . pisi . (B) HILIC-LC-ESI-MS/MS analysis of aq-methanolic (MeOH:chloroform:H 2 O; 40:40:20, v/v/v) extracts. Negative mode [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line; amplified by a factor of 10), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed.

    Journal: PLoS ONE

    Article Title: Synthesis of UDP-apiose in Bacteria: The marine phototroph Geminicoccus roseus and the plant pathogen Xanthomonas pisi

    doi: 10.1371/journal.pone.0184953

    Figure Lengend Snippet: Detection of bUAS transcripts and UDP-apiose. In vivo indication for the functional activity of bUAS genes and enzymes. (A) RT-PCR analyses showing bUAS transcripts of G . roseus and X . pisi . (B) HILIC-LC-ESI-MS/MS analysis of aq-methanolic (MeOH:chloroform:H 2 O; 40:40:20, v/v/v) extracts. Negative mode [M-H] - ions diagnostic for UDP-pentose ( m/z 535.0, solid line; amplified by a factor of 10), UDP-hexuronic acid ( m/z 579.0, dashed line) and Park’s nucleotide ( m/z 595.6, dotted line) are displayed.

    Article Snippet: HILIC-LC-ESI-MS/MS ESI-MS/MS analysis was performed on a Shimadzu (Kyoto, Japan) LC-ESI-MS-IT-TOF operating in the negative ion mode.

    Techniques: In Vivo, Functional Assay, Activity Assay, Reverse Transcription Polymerase Chain Reaction, Hydrophilic Interaction Liquid Chromatography, Mass Spectrometry, Diagnostic Assay, Amplification

    Analysis of CGA and CSF samples in the rats treated with water extract of E. ulmoides (EUWE) by UHPLC-ESI-MS/MS chromatogram. ( A ) Positive Scan m / z 100–500 of CGA; ( B ) Negative Scan m / z 100–500 of CGA; ( C ) MS/MS spectrum of m / z 353.10 of CGA in the negative ion mode; ( D ) MRM of channels ESI − 353.10/191.15 for CGA and CSF samples in rat treated with EUWE; ( E ) MRM of channels ESI − 353.10/179.00 for CGA and CSF samples in the rat treated with EUWE. Notes: chromatographic conditions are provided in the Experimental Section. The rats were treated with EUWE at 4.0 g/kg.

    Journal: Molecules

    Article Title: Antidepressant Potential of Chlorogenic Acid-Enriched Extract from Eucommia ulmoides Oliver Bark with Neuron Protection and Promotion of Serotonin Release through Enhancing Synapsin I Expression

    doi: 10.3390/molecules21030260

    Figure Lengend Snippet: Analysis of CGA and CSF samples in the rats treated with water extract of E. ulmoides (EUWE) by UHPLC-ESI-MS/MS chromatogram. ( A ) Positive Scan m / z 100–500 of CGA; ( B ) Negative Scan m / z 100–500 of CGA; ( C ) MS/MS spectrum of m / z 353.10 of CGA in the negative ion mode; ( D ) MRM of channels ESI − 353.10/191.15 for CGA and CSF samples in rat treated with EUWE; ( E ) MRM of channels ESI − 353.10/179.00 for CGA and CSF samples in the rat treated with EUWE. Notes: chromatographic conditions are provided in the Experimental Section. The rats were treated with EUWE at 4.0 g/kg.

    Article Snippet: UHPLC-ESI-MS/MS Analysis The UHPLC-ESI-MS/MS analyses were performed on a Shimadzu LCMS-8040 UHPLC system comprised of two LC-30AD pumps, a SIL-30AC autosampler with a CTO-30AC column oven, a DGU-20A5 degasser, a Shimadzu CBM-20A system controller, a Labsolution LCMS Ver.5.75 workstation, an ESI ion source and a LCMS-8040 mass spectrometer.

    Techniques: Mass Spectrometry

    UHPLC-ESI-MS/MS chromatogram of CGA and toosendanin (TSN). ( A ) Negative Scan m / z 100–500 of CGA; ( B ) MS/MS spectrum of m / z 353.10 in the negative ion mode; ( C ) Negative Scan m / z 100–700 of TSN; ( D ) MS/MS spectrum of m / z 573.10 in the negative ion mode; ( E ) MRM of channels ESI − 353.10/191.15 and 353.10/161.10 for blank CSF sample; ( F ) MRM of channels ESI − 353.10/191.15 and 353.10/161.10 for CSF spiked with CGA; ( G ) MRM of channels ESI − 573.15/531.15 for blank CSF sample; ( H ) MRM of channels ESI − 573.15/531.15 for CSF spiked with TSN. Notes: Chromatographic conditions are provided in the Experimental Section.

    Journal: Molecules

    Article Title: Antidepressant Potential of Chlorogenic Acid-Enriched Extract from Eucommia ulmoides Oliver Bark with Neuron Protection and Promotion of Serotonin Release through Enhancing Synapsin I Expression

    doi: 10.3390/molecules21030260

    Figure Lengend Snippet: UHPLC-ESI-MS/MS chromatogram of CGA and toosendanin (TSN). ( A ) Negative Scan m / z 100–500 of CGA; ( B ) MS/MS spectrum of m / z 353.10 in the negative ion mode; ( C ) Negative Scan m / z 100–700 of TSN; ( D ) MS/MS spectrum of m / z 573.10 in the negative ion mode; ( E ) MRM of channels ESI − 353.10/191.15 and 353.10/161.10 for blank CSF sample; ( F ) MRM of channels ESI − 353.10/191.15 and 353.10/161.10 for CSF spiked with CGA; ( G ) MRM of channels ESI − 573.15/531.15 for blank CSF sample; ( H ) MRM of channels ESI − 573.15/531.15 for CSF spiked with TSN. Notes: Chromatographic conditions are provided in the Experimental Section.

    Article Snippet: UHPLC-ESI-MS/MS Analysis The UHPLC-ESI-MS/MS analyses were performed on a Shimadzu LCMS-8040 UHPLC system comprised of two LC-30AD pumps, a SIL-30AC autosampler with a CTO-30AC column oven, a DGU-20A5 degasser, a Shimadzu CBM-20A system controller, a Labsolution LCMS Ver.5.75 workstation, an ESI ion source and a LCMS-8040 mass spectrometer.

    Techniques: Mass Spectrometry

    Effects of chronic running exercise on α-oxoaldehydes in Zucker diabetic fatty rats. (A) Urinary methylglyoxal (MG) in sedentary lean Zucker diabetic fatty (ZDF) rats (Sed-ZL), sedentary ZDF rats (Sed-ZDF) and aerobic treadmill exercised ZDF rats (Ex-ZDF) groups was assessed by electrospray ionization (ESI) liquid chromatography (LC)-mass spectrometry (MS) (LC-MS/MS), and expressed as urinary MG:creatinine ratio. (B) MG, (C) 3-deoxy-D-glucosone (3DG) and (D) glyoxal (GO) in the renal cortex of Sed-ZL, Sed-ZDF and Ex-ZDF groups were assessed by LC-MS/MS. Values are means ± SEM ( n = 5/group). * p

    Journal: PLoS ONE

    Article Title: Chronic Running Exercise Alleviates Early Progression of Nephropathy with Upregulation of Nitric Oxide Synthases and Suppression of Glycation in Zucker Diabetic Rats

    doi: 10.1371/journal.pone.0138037

    Figure Lengend Snippet: Effects of chronic running exercise on α-oxoaldehydes in Zucker diabetic fatty rats. (A) Urinary methylglyoxal (MG) in sedentary lean Zucker diabetic fatty (ZDF) rats (Sed-ZL), sedentary ZDF rats (Sed-ZDF) and aerobic treadmill exercised ZDF rats (Ex-ZDF) groups was assessed by electrospray ionization (ESI) liquid chromatography (LC)-mass spectrometry (MS) (LC-MS/MS), and expressed as urinary MG:creatinine ratio. (B) MG, (C) 3-deoxy-D-glucosone (3DG) and (D) glyoxal (GO) in the renal cortex of Sed-ZL, Sed-ZDF and Ex-ZDF groups were assessed by LC-MS/MS. Values are means ± SEM ( n = 5/group). * p

    Article Snippet: Quantitative analysis of the derivatized α-oxoaldehydes by electrospray ionization (ESI) liquid chromatography (LC)-MS/MS was performed with a Nexera Quanternary LC Systems (Shimadzu Co., Tokyo, Japan) coupled to a TSQ Quantum Ultra (Thermo Fisher Scientific) and operated in the positive ion mode.

    Techniques: Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

    Total ion chromatorgraphies of CS aqueous extract by HPLC-ESI-MS/MS analysis. (a) Positive ion mode. (b) Negative ion mode.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Aqueous Extract of Clerodendranthus spicatus Exerts Protective Effect on UV-Induced Photoaged Mice Skin

    doi: 10.1155/2016/9623957

    Figure Lengend Snippet: Total ion chromatorgraphies of CS aqueous extract by HPLC-ESI-MS/MS analysis. (a) Positive ion mode. (b) Negative ion mode.

    Article Snippet: HPLC-ESI-MS/MS Analysis of CS Aqueous Extract HPLC analysis was executed on a Shimadzu LC-20A HPLC system consisting of a SPD-M20A PDA detector, a LC-20AD pump, a SIL-20AC automatic sampler, and CTO-20A thermostatic column compartment (Shimadzu Kyoto, Japan).

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry