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Agilent technologies electrophoresis device
Electrophoresis Device, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electrophoresis device/product/Agilent technologies
Average 93 stars, based on 1 article reviews
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electrophoresis device - by Bioz Stars, 2020-05
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Electrophoresis:

Article Title: Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells
Article Snippet: .. RNA concentration was measured by NanoDrop ND1000 spectrophotometer (NanoDropTechnologies; Thermo Scientific™, LLC, Wilmington, DE, USA), and RNA integrity was assessed using an automated electrophoresis device (Agilent 2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). ..

Concentration Assay:

Article Title: Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells
Article Snippet: .. RNA concentration was measured by NanoDrop ND1000 spectrophotometer (NanoDropTechnologies; Thermo Scientific™, LLC, Wilmington, DE, USA), and RNA integrity was assessed using an automated electrophoresis device (Agilent 2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). ..

Spectrophotometry:

Article Title: Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells
Article Snippet: .. RNA concentration was measured by NanoDrop ND1000 spectrophotometer (NanoDropTechnologies; Thermo Scientific™, LLC, Wilmington, DE, USA), and RNA integrity was assessed using an automated electrophoresis device (Agilent 2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). ..

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  • 99
    Agilent technologies microarray analysis
    Global transcriptional analysis of JS95 in the presence of SilCR. To identify genes whose transcription is affected by SilCR, we exposed strain JS95 and its Δ silAB mutant to the conditions specified, and performed transcriptome analyses using a “universal” GAS <t>microarray,</t> as detailed in “ Materials and Methods ”. The results represent gene loci whose expression ratio changed by 2-fold or more with a p-value
    Microarray Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 1221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray analysis/product/Agilent technologies
    Average 99 stars, based on 1221 article reviews
    Price from $9.99 to $1999.99
    microarray analysis - by Bioz Stars, 2020-05
    99/100 stars
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    86
    Agilent technologies dna microarray analysis a rat
    Workflow from sampling and grinding of the hippocampus, total RNA extraction, and <t>DNA</t> <t>microarray</t> analysis of the hippocampus. (A) The rats hippocampi were rapidly dissected and ground to a fine powder in liquid nitrogen and stored at –80°C. (B) Total RNA extraction from the finely powdered hippocampus. Total RNA quality was confirmed by both spectrophotometry and agarose‐gel electrophoresis. (C) DNA microarray chip showing the hybridized sample combinations (Sed × WR and Sed × RWR) and dye‐swap (Cy3 vs. Cy5).
    Dna Microarray Analysis A Rat, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray analysis a rat/product/Agilent technologies
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna microarray analysis a rat - by Bioz Stars, 2020-05
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    93
    Agilent technologies dna microarray analysis
    Workflow from sampling and grinding of the brain hemisphere, total RNA extraction, and <t>DNA</t> <t>microarray</t> analysis of the ischemic brain (ipsilateral hemisphere). (A) Mouse whole brain showing the ipsilateral (right; boxed region) and contralateral (left) hemispheres. Brain tissues were ground to a fine powder in liquid nitrogen and stored at −80°C. (B) Total RNA extraction from the finely powdered brain tissues. Total RNA quality was confirmed by both agarose-gel electrophoresis and NanoDrop spectrophotometry. (C) DNA microarray chip showing the hybridized sample combinations (Sham × PMCAO at 6 and 24 hours) and dye-swap (Cy3 vs Cy5).
    Dna Microarray Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna microarray analysis/product/Agilent technologies
    Average 93 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    dna microarray analysis - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Global transcriptional analysis of JS95 in the presence of SilCR. To identify genes whose transcription is affected by SilCR, we exposed strain JS95 and its Δ silAB mutant to the conditions specified, and performed transcriptome analyses using a “universal” GAS microarray, as detailed in “ Materials and Methods ”. The results represent gene loci whose expression ratio changed by 2-fold or more with a p-value

    Journal: PLoS Pathogens

    Article Title: Functional Analysis of the Quorum-Sensing Streptococcal Invasion Locus (sil)

    doi: 10.1371/journal.ppat.1000651

    Figure Lengend Snippet: Global transcriptional analysis of JS95 in the presence of SilCR. To identify genes whose transcription is affected by SilCR, we exposed strain JS95 and its Δ silAB mutant to the conditions specified, and performed transcriptome analyses using a “universal” GAS microarray, as detailed in “ Materials and Methods ”. The results represent gene loci whose expression ratio changed by 2-fold or more with a p-value

    Article Snippet: For microarray analysis the RNA concentration and integrity was verified by capillary gel electrophoresis in the Agilent 2100 Bioanalyzer.

    Techniques: Mutagenesis, Microarray, Expressing

    Analysis of behavior of PLAC8 , LAMP1 , PLA2G7 , and CEACAM4 in the discovery cohort. (A) Heat map representation of the discovery cohort (74 cases, 31 controls). Normalized expression levels of the individual genes comprising the SeptiCyte Lab classifier (color) are plotted versus disease status (dendrogram position) using unsupervised clustering with equally weighted Euclidean distance. The normalization scale (expression level Z -score) for up-regulation (red) or down-regulation (green) is shown in the insert at the left of the heat map. (The Z- score is the number of standard deviations a value lies away from the mean. Higher absolute Z- scores correspond to lower p- values. A Z- score of ±1.96 equates to a p- value of 0.05 in a two-tailed test.) In the cases (sepsis), two genes are predominately up-regulated ( PLAC8 and LAMP1 ), whilst two are predominantly down regulated ( PLA2G7 and CEACAM4 ). (B) Scatterplot representation of microarray expression levels for individual genes in the SeptiCyte Lab classifier, for the discovery cohort. The expression level on log 2 scale ( y- axis) is presented for PLAC8 , CEACAM4 , LAMP1 , and PLA2G7 in individual patients (red for cases, black for controls). Each gene contributes to the ability of the SeptiCyte Lab classifier to separate the cases and controls. S.I., systemic inflammation.

    Journal: PLoS Medicine

    Article Title: A Molecular Host Response Assay to Discriminate Between Sepsis and Infection-Negative Systemic Inflammation in Critically Ill Patients: Discovery and Validation in Independent Cohorts

    doi: 10.1371/journal.pmed.1001916

    Figure Lengend Snippet: Analysis of behavior of PLAC8 , LAMP1 , PLA2G7 , and CEACAM4 in the discovery cohort. (A) Heat map representation of the discovery cohort (74 cases, 31 controls). Normalized expression levels of the individual genes comprising the SeptiCyte Lab classifier (color) are plotted versus disease status (dendrogram position) using unsupervised clustering with equally weighted Euclidean distance. The normalization scale (expression level Z -score) for up-regulation (red) or down-regulation (green) is shown in the insert at the left of the heat map. (The Z- score is the number of standard deviations a value lies away from the mean. Higher absolute Z- scores correspond to lower p- values. A Z- score of ±1.96 equates to a p- value of 0.05 in a two-tailed test.) In the cases (sepsis), two genes are predominately up-regulated ( PLAC8 and LAMP1 ), whilst two are predominantly down regulated ( PLA2G7 and CEACAM4 ). (B) Scatterplot representation of microarray expression levels for individual genes in the SeptiCyte Lab classifier, for the discovery cohort. The expression level on log 2 scale ( y- axis) is presented for PLAC8 , CEACAM4 , LAMP1 , and PLA2G7 in individual patients (red for cases, black for controls). Each gene contributes to the ability of the SeptiCyte Lab classifier to separate the cases and controls. S.I., systemic inflammation.

    Article Snippet: For microarray analysis, additional checks on RNA integrity were performed by microfluidic electrophoresis on (1) an Agilent Bioanalyzer 2100, using the Agilent Bioanalyzer 2100 Nano Assay, or (2) a Caliper LabChip system (Agilent Technologies).

    Techniques: Expressing, Two Tailed Test, Microarray

    Workflow from sampling and grinding of the hippocampus, total RNA extraction, and DNA microarray analysis of the hippocampus. (A) The rats hippocampi were rapidly dissected and ground to a fine powder in liquid nitrogen and stored at –80°C. (B) Total RNA extraction from the finely powdered hippocampus. Total RNA quality was confirmed by both spectrophotometry and agarose‐gel electrophoresis. (C) DNA microarray chip showing the hybridized sample combinations (Sed × WR and Sed × RWR) and dye‐swap (Cy3 vs. Cy5).

    Journal: Physiological Reports

    Article Title: DNA microarray‐based analysis of voluntary resistance wheel running reveals novel transcriptome leading robust hippocampal plasticity

    doi: 10.14814/phy2.12206

    Figure Lengend Snippet: Workflow from sampling and grinding of the hippocampus, total RNA extraction, and DNA microarray analysis of the hippocampus. (A) The rats hippocampi were rapidly dissected and ground to a fine powder in liquid nitrogen and stored at –80°C. (B) Total RNA extraction from the finely powdered hippocampus. Total RNA quality was confirmed by both spectrophotometry and agarose‐gel electrophoresis. (C) DNA microarray chip showing the hybridized sample combinations (Sed × WR and Sed × RWR) and dye‐swap (Cy3 vs. Cy5).

    Article Snippet: DNA microarray analysis A rat 4 × 44K whole genome oligo DNA microarray chip (G4131F, Agilent Technologies, Palo Alto, CA) was used for global gene expression analysis using the hippocampi.

    Techniques: Sampling, RNA Extraction, Microarray, Spectrophotometry, Agarose Gel Electrophoresis, Chromatin Immunoprecipitation

    Workflow from sampling and grinding of the brain hemisphere, total RNA extraction, and DNA microarray analysis of the ischemic brain (ipsilateral hemisphere). (A) Mouse whole brain showing the ipsilateral (right; boxed region) and contralateral (left) hemispheres. Brain tissues were ground to a fine powder in liquid nitrogen and stored at −80°C. (B) Total RNA extraction from the finely powdered brain tissues. Total RNA quality was confirmed by both agarose-gel electrophoresis and NanoDrop spectrophotometry. (C) DNA microarray chip showing the hybridized sample combinations (Sham × PMCAO at 6 and 24 hours) and dye-swap (Cy3 vs Cy5).

    Journal: Disease Models & Mechanisms

    Article Title: Unraveling the ischemic brain transcriptome in a permanent middle cerebral artery occlusion mouse model by DNA microarray analysis

    doi: 10.1242/dmm.008276

    Figure Lengend Snippet: Workflow from sampling and grinding of the brain hemisphere, total RNA extraction, and DNA microarray analysis of the ischemic brain (ipsilateral hemisphere). (A) Mouse whole brain showing the ipsilateral (right; boxed region) and contralateral (left) hemispheres. Brain tissues were ground to a fine powder in liquid nitrogen and stored at −80°C. (B) Total RNA extraction from the finely powdered brain tissues. Total RNA quality was confirmed by both agarose-gel electrophoresis and NanoDrop spectrophotometry. (C) DNA microarray chip showing the hybridized sample combinations (Sham × PMCAO at 6 and 24 hours) and dye-swap (Cy3 vs Cy5).

    Article Snippet: DNA microarray analysis in the ipsilateral (right) hemisphere A mouse 4x44K whole genome oligo DNA microarray chip (G4122F, Agilent Technologies, Palo Alto, CA) was used for global gene expression analysis using the ipsilateral (ischemic) hemisphere.

    Techniques: Sampling, RNA Extraction, Microarray, Agarose Gel Electrophoresis, Spectrophotometry, Chromatin Immunoprecipitation